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All Journal Journal of Food and Pharmaceutical Science Pharmascience Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Window of Health : Jurnal Kesehatan Jurnal Mandala Pharmacon Indonesia Abdimas Galuh: Jurnal Pengabdian Kepada Masyarakat Hydrogen: Jurnal Kependidikan Kimia Mejuajua Pharmaceutical Reports Journal Microbiology Science Journal of Food and Pharmaceutical Sciences Indonesian Journal of Jamu MPSJ
Selpida Handayani, Selpida
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Journal : Journal Microbiology Science

 1 
Antibacterial Activity of Ethanol Extract of Purple Kencana Leaves (Ruellia tuberosa L.) Using TLC-Bioautography and Agar Diffusion Methods Sari, Felisa Ananda; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 3, No 2 (2023)
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v3i2.982

Abstract

Kencana Ungu Leaf (Ruellia tuberosa L.) is a traditional plant that has antibacterial activity. The purpose of this study was to determine the antibacterial activity of the ethanol extract of Kencana Ungu leaves (Ruellia tuberosa L.) using TLC-Bioautography and Agar Diffusion methods. Simplisia from Kencana Ungu leaves (Ruellia tuberosa L.) was extracted by maceration method and then evaporated to obtain a thick extract. Preliminary testing, namely the antibacterial screening test, active extract at a concentration of 0.5% against Escherichia coli, Shigella dysenteriae, and Salmonella thypi. The results of the antibacterial activity test using the TLC-Bioautography method using chloroform: methanol (7:1) as an eluent showed results with an Rf value of 0.89 which inhibited the growth of Escherichia coli, Shigella dysenteriae, and Salmonella thypi bacteria. While the results of testing the antibacterial activity using the Agar Diffusion method obtained the largest diameter of the inhibition zone at a concentration of 20% for Escherichia coli bacteria with an inhibition zone diameter of 12.15 mm, Shigella dysenteriae with an inhibition zone diameter of 11.72 mm, and Salmonella thypi with an inhibition zone diameter of 12.67 mm
Isolation of Endophytic Fungus from Bay Leaves (Syzygium polyanthum Wight.) as Antibacterials Against Bacteria Causing Digestive Tract Infections by Bioautography-TLC Fahirah, Jihan; Herwin, Herwin; Handayani, Selpida
Journal Microbiology Science Vol 5, No 2 (2025): Volume 5(2) 2025
Publisher : Fakultas Farmasi, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56711/jms.v5i2.1301

Abstract

Salam (Syzygium polyanthum (Wight) Walp), belonging to the Myrtaceae family, are noted for their potential antibacterial properties targeting bacteria linked to gastrointestinal infections. This study aims to identify endophytic fungus isolates from bay leaves that produce antibacterial compounds effective against bacteria responsible for gastrointestinal infections, utilizing TLC-Bioautography as a method of analysis. The endophytic fungus from bay leaves was isolated, yielding 17 pure isolates. The findings from the antagonist test of pure isolates of endophytic fungus revealed two isolates with the most significant inhibitory zone diameters: IFSP-8 and IFSP-15. These isolates demonstrated effectiveness against Escherichia coli, Salmonella typhi, Shigella dysenteriae, and Vibrio cholerae bacteria. The active isolate was then extracted by liquid-liquid extraction to obtain ethyl acetate extract for the IFSP-8 and IFSP-15 isolates. The antibacterial activity results of the ethyl acetate extract, analyzed through TLC-Bioautography with a chloroform: methanol (9:1) eluent, indicated the values of Rf1 0,76; Rf2 0,56; Rf3 0,47 for the IFSP-8 isolate. In contrast, the IFSP-15 isolate exhibited the values of Rf1 0,81; Rf2 0,69; Rf3 0,56, demonstrating inhibition of bacteria responsible for gastrointestinal infections.
Co-Authors Abdul Rasyid, Faradiba Amaliah Dahlia, Andi Aminah Aminah Amran, Farizah Dhaifina Anasya, Andi Fara Andina, Velda Annisa, Khairun Aulia Wati, Aulia Badu, Nurul Fadillah Fahirah, Jihan Faradiba Hasnaeni Hasnaeni hasnaeni, Hasnaeni Herwin Herwin, Herwin Hossani, Rahni Ida Kurniawati, Ida Imani, Fitril Indah Indah Indah Pratiwi Lalo, Ahmad Majid, Nini Nirmala Mamat Pratama, Mamat Nonci, Faridha Yenni Nurliadi, Indah Nurlina Nurlina Nurlina Putri, Andra Audina Putri, Aulia Dinda Rahmawati, Junita Eka Rais Razak, Rais Rezki Amriati Syarif, Rezki Amriati Risda Waris, Risda Sari, Felisa Ananda Syahrana, Nur Azizah Tahir, Karlina Amir Utami, Fadhillah Resky Virsa Handayani Wenni, Asyam Hafidz Wisdawati Wisdawati, Wisdawati