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All Journal Jurnal Sains dan Teknologi Farmasi Biology, Medicine, & Natural Product Chemistry JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Syntax Literate: Jurnal Ilmiah Indonesia Martabe : Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) Jurnal Ipteks Terapan Logista: Jurnal Ilmiah Pengabdian Kepada Masyarakat Insight: Jurnal Bimbingan dan Konseling Jurnal Pengabdian Harapan Ibu (JPHI) BioEksakta : Jurnal Ilmiah Biologi Unsoed Khazanah Intelektual Jurnal Ipteks Terapan : research of applied science and education Jurnal Ilmiah Bakti Farmasi pendidikan, science, teknologi, dan ekonomi Jurnal Katalisator Riset Informasi Kesehatan Jurnal Pengabdian Harapan Ibu (JPHI) Kartika : Jurnal Ilmiah Farmasi

Desi Sagita

Universitas Adiwangsa Jambi Jl. Sersan Muslim RT.24 Kebun Kopi Kelurahan Tehok Jambi

Author-ID : 638606

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The KARAKTERISASI ENZIM Β-SIKLODEKSTRIN DARI ISOLAT LOKAL (BTS3A, BTS3-B DAN CK-2) PROVINSI JAMBI Desi Sagita; Rahmadevi
JURNAL KATALISATOR Vol. 6 No. 1 (2021): jurnal Katalisator Volume 6 No 1, 2021
Publisher : LLDIKTI X Sumbar, Riau, Jambi, Kepri

The β-cyclodextrin glucosyltransferase (β-CGTase) enzyme is an extracellular enzyme that converts starch to cyclodextrins. Cyclodextrins are widely used in the pharmaceutical industry, one of which is to increase solubility. This enzyme is produced by bacteria, especially the genus Bacillus sp. BTS3A, BTS3B and CK-2 isolates that produce CGTase enzymes have been isolated from the soil of cassava and potato plantations. The bacteria are fermented in liquid Horikoshi media, then centrifuged at 4 ° C and then concentrated by freeze dry. The enzyme was purified by the ammonium sulfate precipitation method up to 59.61% for BTS3A, 59.6% for BTS3B and 58.1% for CK-2. Then dialyzed using a cellophane membrane with cut off 14 KDa in phosphate buffer. The cyclization activity of crude enzymes was characterized by varying incubation times of 30, 60, 120 minutes and incubation temperatures of 30 ℃, 37 ℃, 55 ℃. CGTase cyclization activity was measured based on the decrease in phenolphthalein absorbance at a wavelength of 550 nm. The β-CGTase enzyme from the three isolates showed the optimum percentage reduction at 55°C for 30 minutes incubation

Potensi Lidah Mertua Dalam Menghambat Pertumbuhan Bakteri Salmonella sp Dan Staphylococcus aureus Desi Sagita; Siti Hamidatul Aliyah; Mery Safitri
Riset Informasi Kesehatan Vol 7 No 2 (2018): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Abstrak Latar Belakang : Lidah mertua (Sansevieria trifasciata Prain) sering dikenal dengan nama Snake plant (tanaman ular) merupakan kelompok tanaman yang dibudidayakan masyarakat sebagai tanaman hias. Telah banyak penelitian yang dilakukan terhadap daun dan rhizome Sansevieria trifasciata Prain ini menunjukkan adanya kandungan senyawa yang memiliki aktivitas farmakologi, yaitu saponin, tannin, flavonoid, dan glikosida. Ekstrak tanaman ini memberikan aktivitas antibakteri, antialergi, antianafilaksis. Dari studi literatur kebanyakan penelitian dari tanaman Sansevieria trifasciata Prain menggunakan ektraks daun maupun rhizome. Berdasarkan hal tersebut maka pada penelitian ini dilakukan pengujian fraksi non polar, semi polar dan polar daun Sansevieria trifasciata Prain untuk menghambat pertumbuhan bakteri Salmonella sp dan Staphylococus aureus. Metode : Proses penyarian tanaman Lidah mertua melalui proses maserasi selama 3 hari dengan 3 kali pengulangan dengan pelarut etanol. pengujian antibakteri menggunakan metode difusi cakram. Penelitian ini menggunakan konsentrasi 30%, 40%, 50%, 60% dan kontrol positif kloramfenikol. Hasil : Hasil penelitian menunjukkan bahwa fraksi n-heksana dan fraksi n- butanol menghambat pertumbuhan bakteri Salmonella sp dan Staphylococus aureus sementara fraksi etil asetat tidak memberikan aktivitas antibakteri. Fraksi n-butanol mampu menghambat pertumbuhan bakteri Salmonella sp dan Staphylococcus aureus lebih besar dibandingkan fraksi lainnya. Fraksi n-butanol mampu menghambat pertumbuhan bakteri dimulai konsentrasi 30%. Hal ini dikarenakan fraksi n-butanol mengandung senyawa aktif antibakteri lebih banyak. Kesimpulan : Fraksi n-butanol mampu menghambat pertumbuhan bakteri Salmonella sp dan Staphylococcus aureus lebih besar dibandingkan fraksi lainnya. Semakin tinggi konsentrasi ekstrak maka semakin besar zona bening yang terbentuk

Pemanfaatan Tanaman Tradisional Sebagai Penunjang Kesehatan di Kelurahan Olak Kemang Kecamatan Danau Teluk Kota Jambi Santi Perawati; Lia Anggresani; Yuni Andriani; Lili Andriani; Barmi Hartesi; Rahmadevi Rahmadevi; Desi Sagita; Deny Sutrisno; Fhatia Medina; Muhammad Randa; Indah Astridawati
Jurnal Pengabdian Harapan Ibu (JPHI) Vol 2 No 1 (2020): Jurnal Pengabdian Harapan Ibu (JPHI)
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Latar Belakang:Survei dan sosialisasi tentang pemanfaatan tanaman tradisional, penggunaan obat sintetis yang baik dan benar, bahaya kosmetik ilegal dan kanker serviks dilakukan atas dasar menyadarkan kembali kepada masyarakat akan pentingnya pengobatan yang berasal dari alam, bahaya penggunaan kosmetik yang tidak terdaftar serta cara mendeteksi kanker serviks khususnya pada wanita. Penggunaan obat sintetis dipilih jika suatu penyakit tidak dapat diatasi oleh obat-obatan tradisional. Selain itu, perlu diimbau kembali kepada masyarakat khususnya para wanita agar mewaspadai kosmetik ilegal yang masih beredar karena bahaya yang ditimbulkan akan sangat serius di kemudian hari. Adapun pengenalan tentang kanker serviks dimana penyakit ini masih menjadi ancaman yang mematikan bagi kaum wanita dikarenakan sulitnya untuk mendeteksi penyakit ini. Metode:Data survei dikumpulkan melalui pemberian kuesioner ke masing-masing rumah warga.Menindaklanjuti hasil survei, maka diadakan sosialisasi yang disampaikan dengan metode ceramah dan tanya jawab seputar tema yang dibicarakan Hasil: Hasil survei didapatkan bahwa pertama, warga belum banyak mengetahui kegunaan atau cara memanfaatkan tanaman di sekitar rumah mereka untuk tujuan pengobatan. Kedua, warga sebagian besar tidak mengetahui cara membuang obat yang benar, ketiga para wanita atau ibu-ibu di RT.06 masih ada memakai kosmetik yang ternyata mengandung zat berbahaya dan keempat para wanita atau ibu di RT.06 belum mengetahui apa itu kanker serviks dan bagaimana cara mendeteksi penyakit ini. Kesimpulan:setelah diadakannya sosialisasi masyarakat telah mengetahui manfaat tanaman tradisional, informasi seputar penggunaan obat, bahaya kosmetik ilegal, dan cara mendeteksi sejak dini kanker serviks.

Pembentukan Pembentukan Kompleks Inklusi Ibuprofen Kombinasi Polimer beta siklodekstrin dan Hydroxypropyl Metylcelulose Menggunakan Teknik Kneading HARTESI HARTESI; Lia Anggresani; Desi Sagita; Julia Ambar Sari
Riset Informasi Kesehatan Vol 7 No 1 (2018): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v7i1.139

Latar Belakang : Ibuprofen termasuk kedalam BCS kelas II yaitu memiliki kelarutan yang rendah tetapi memiliki permeabilitas tinggi.Berdasarkan literatur kombinasi beta siklodekstrin dan Hidroksipropil Metilselulosa dapat meningkatkan kelarutan. Tujuan :Untuk meningkatkan laju disolusi dari ibuprofen dalam bentuk kompleks inklusi dengan kombinasi beta siklodekstrin dan Hidroksipropil Metilselulosa Metoda :Menggunakan teknik kneading. Ibuprofen di buat dengan 4 formula dengan perbandingan beta siklodekstrin F1 (1:0,5), F2 (1:1), F3 (1:1.5), dan F4 (1:2) dan penambahan Hidroksipropil Metilselulosa 0.1 % dari jumlah formula. Formula yang telah dibuat selanjutnya dilakukan evaluasi yang meliputi persen perolehan kembali, laju disolusi, ,distribusi ukuran partikel, analisa FTIR, XRD dan SEM. Hasil :Hasil dari evaluasi menunjukan bahwa semua formula memenuhi persyaratan dengan penambahan beta siklodekstrin dan Hidroksipropil Metilselulosa kecuali formula 1. Hasil profil disolusi memperlihatkan bahwa adanya peningkatan laju disolusi. Kesimpulan :Dapat disimpulkan bahwa kompleks inklusi ibuprofen dengan beta siklodekstrin dan Hidroksipropil Metilselulosa menggunakan teknik kneading dapat meningkatkan laju disolusi yang dapat dilihat dengan semakin banyak penambahan beta siklodekstrin dan Hidroksipropil Metilselulosa maka akan semakin tinggi kelarutan dan laju disolusinya. Formula yang paling baik yaitu formula 4 dengan perbandingan 1 : 2 (Ibuprofen : Beta siklodekstrin) dan penambahan Hidroksipropil Metilselulosa 0.1 % dari jumlah formula. Kata Kunci :Kompleks Inklusi, Ibuprofen, Î²- siklodekstrin, Hidroksipropil Metilselulosa, Kneading.

Skrining aktifitas antibakteri dari ekstrak Sisik Naga (Pyyrosia piloselloides (L) M.G.Price) desi sagita; Muhammad Nurul Ichwani; Linuria Linuria
Riset Informasi Kesehatan Vol 6 No 2 (2017): Riset Informasi Kesehatan
Publisher : Sekolah Tinggi Ilmu Kesehatan Harapan Ibu Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30644/rik.v6i2.101

Abstract Background : Sisik naga herbs (Pyyrosiapiloselloides (L) M.G. Price) is an epiphyte plants with compounds of flavonoids and tannins. The compound provides antibacterial potency. This plant is effective for wounds, canker sores, constipation and dysentery. The antibacterial activity of the plant is potentially for development of medicinal plant compounds. The extraction process by maceration for 3 days with three repetitions. From 1 kg of weight of Sisik naga obtained 4.95% extract. Antibacterial test using the dilution methods. The results showed the Sisik naga herbs provide antibacterial activity with MIC 256 Î¼g/mL and MBC 512 Î¼g/mL against bacteria Pseudomonas aeruginosa and Staphylococcus aureus. As a positive control used a solution of chloramphenicol.(Objective) This research is aimed to knowing the antibacterial activity of ethanol extracts from Sisik naga hersb. Method : The extraction process by maceration for 3 days with three repetitions with ethanol. Antibacterial avtivity tes by dilution methods. Results :. From 1 kg of Sisik naga obtained 4.95% extract.. The results showed the Sisik naga herbs provide antibacterial activity with MIC 256 Î¼g/mL and MBC 512 Î¼g/mL against bacteria Pseudomonas aeruginosa and Staphylococcus aureus. As a positive control used a solution of chloramphenicol Conclusion : herb Sisik naga extracts showed antibacterial activity to Pseudomonas aeruginosae and Staphylococcus aureus Key words: antibacterial, sisik naga , dilution methods

STUDI ETNOFARFARMASI PADA SUKU ANAK DALAM (SAD) DI DESA PEMAYONGAN KECAMATAN SUMAY KABUPATEN TEBO PROVINSI JAMBI: STUDI ETNOFARFARMASI PADA SUKU ANAK DALAM (SAD) DI DESA PEMAYONGAN KECAMATAN SUMAY KABUPATEN TEBO PROVINSI JAMBI Perawati, Santi; Sutrisno, Deny; Ara Ofiana; Deny Sutrisno; Siti Hamidatul 'Aliyah; Lili Andriani; Rizky Yulion Putra; Rahmadevi; Desi Sagita; Yuni Andriani; Lia Anggresani; Barmi Hartesi
Jurnal Khazanah Intelektual Vol. 7 No. 2 (2023): Khazanah Intelektual
Publisher : Balitbangda Provinsi Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37250/khazanah.v7i2.198

Background : Suku Anak Dalam is one of the tribes in Indonesia who still upholds their customs and culture, including their local knowledge about medicine using medicinal plants. One of the Suku Anak Dalam community lives in Pemayongan Village, Tebo Regency, Jambi Province. This ethnopharmaceutical research was conducted to find out various diseases experienced by Suku Anak Dalam and to know various natural resources that are used as treatment. Method : This type of research is a descriptive study using qualitative methods and sampling techniques, namely snowball sampling through open-ended interviews with informants. Results: The results of the study stated that diseases that was often experienced by the Suku Anak Dalam included fever, coughing, itching, shortness of breath and abdominal pain. Treatment of the disease by utilizing natural resources in the form of plants and animals. Plant parts used are roots, leaves, bark, and fruit sap, while for animals the parts used are fat, scales, and meat. The processing method is scraped, crushed, boiled, fried, while the use of these ingredients is eaten, drunk, and applied to the affected part. Conclusion : Based on the results of research that has been done there are 5 diseases that often occur and there are 9 kinds of plants and 5 animals that are used as traditional medicine. Keywords : Ethnopharmacy, Disease, Jambi, Suku Anak Dalam, Natural Resources

Isolasi dan Karakterisasi Bakteri Penghasil Enzim β-CGTase dari Tanah Perkebunan Ubi Jalar Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi; Andriani, Lili; Dahlia, Dimbi
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 6 No 3 (2024): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2024.6.3.11779

β-CGTase (β-cyclodextrin glycosyl transferase) is an enzyme that converts starch into CD (cyclodextrin). The CD is a high-value oligosaccharide used for modifying food, textiles, chemicals, medicines, cosmetics, and biotechnology. This research aims to obtain isolates of β-CGTase producing bacteria from sweet potato plantation soil. Screening is carried out using Horikoshi media which contains the color indicators phenolphthalein and methyl orange. Bacteria producing β-CGTase will give a yellow hollow zone if they grow on this media. CGTase activity was measured using a UV-Vis spectrophotometer. Three isolates producing CGTase were obtained, coded BSP6A, BSP6B, and BSP6C, which had the characteristics of being Gram-positive, shaped like bacilli, produced spores, were motile, had a positive catalase test, and could ferment acid. These three isolates were able to produce the optimum CGTase enzyme if incubated for 18 and 24 hours at a temperature of 37°C and pH 10 and were able to convert starch into CD if incubated for 30 minutes at a temperature of 37°C and pH 7.

Isolation of b-Cyclodextrin Gycosyl Transferase (b-CGTase) Producing Bacteria from Potato Plantation Soil Kurniadi, Helman; Sagita, Desi; Hartesi, Barmi
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.193-197

Cyclodextrin glycosyltransferase (CGTase) is an extracellular enzyme produced by several microorganisms, particularly bacteria, that converts starch into cyclodextrin (CD). CD has various applications in fields such as food, textiles, pharmaceuticals, chemistry, and biotechnology. The objective of this study was to isolate b-CGTase-producing bacteria from soil in a potato plantation located in Kerinci, and to conduct their characterization. Screening was conducted using Horikoshi agar media with phenolphthalein and methyl orange color indicators. The results indicated that one isolate, coded CK-2, produced the optimum b-CGTase enzyme when incubated for 30 hours at 37 ÂºC and pH 10. The CGTase enzyme converted starch to CD when incubated for 60 minutes at 37 ÂºC and pH 7. The CK-2 isolate was identified as monobacilli Gram-positive bacteria, having spores, positive in the catalase test, and motile. The study concluded that the bacteria producing b-CGTase enzyme are found in the soil of potato plantations in Kerinci, which is believed to originate from the genus Bacillus sp.

KONSENTRASI HAMBAT MINUMUM ENZIM BROMELIN DARI KULIT DAN BONGGOL NANAS (Ananas comosus (L.) Merr) TERHADAP Staphylococcus aureus Sagita, Desi; Hartesi, Barmi; Fitri, Kurnia; Lufita, Lufita
Kartika : Jurnal Ilmiah Farmasi Vol 8 No 2 (2023)
Publisher : Fakultas Farmasi Universitas Jenderal Achmad Yani, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/kjif.v8i2.278

Enzim bromelin adalah enzim proteolitik yang memiliki kemampuan menghidrolisis protein sehingga bisa berperan sebagai antibakteri. Enzim bromelin dapat diisolasi dari kulit, buah, bonggol Nanas (Ananas comosus (L.) Merr ). Tujuan penelitian ini adalah untuk mendapatkan konsentrasi enzim bromelin dari kulit dan bonggol buah Nanas dalam menghambat pertumbuhan Staphylococcus aureus. Bromelin diisolasi dengan cara ekstraksi dengan buffer pospat, dipurifikasi dengan ammonium sulfat 60%. Uji aktivitas antibakteri enzim bromelin dari kulit dan bonggol Nanas terhadap bakteri Staphylococcus aureus dilakukan secara invitro menggunakan difusi cakram dengan mengukur zona hambat. Konsentrasi enzim bromelin yang digunakan adalah 1 %, 2%, 3% dan 4% dengan antibiotik kloramfenikol sebagai kontrol positif. Enzim bromelin dari kulit dan bonggol Nanas pada konsentrasi 4% menghambat pertumbuhan Staphylococcus aureus dengan zona hambat masing masing 19,23 mm dan 18,30 mm. Konsentrasi hambat minimum enzim bromelin dari kulit dan bonggol Nanas adalah 1% dengan zona hambat masing-masing 11,45 mm dan 12,24 mm. Enzim bromelin dari kulit dan bonggol Nanas memberikan aktivitas yang sama dalam menghambat pertumbuhan bakteri Staphylococcus aureus. Kata kunci : Bromelin; Kosentrasi Hambat Minumum; Presipitasi Amonium Sulfat. ABSTRACT The Bromelains are proteolytic enzymes that have the ability to hydrolyze proteins so they can act as antibacterial. Bromelain enzymes can be isolated from the peel, fruit, and stem of pineapple (Ananas comosus (L.) Merr). The purpose of this study was to obtain the concentration of bromelain enzymes from the peel and stem of Pineapple in inhibiting the growth of Staphylococcus aureus. Bromelain was isolated by extraction with phosphate buffer, purified with 60% of ammonium sulfate. Antibacterial activity tests of bromelain enzymes from the peel and stem of pineapple against Staphylococcus aureus were carried out in vitro using disc diffusion by measuring clear zones. The bromelain enzyme concentration used was 1%, 2%, 3% and 4% with chloramphenicol antibiotics as a positive control. The results showed that bromelain enzymes from peel and stem of pineapple at 4% concentration inhibited the growth of Staphylococcus aureus with inhibition zones of 19.23 mm and 18.30 mm respectively. The minimum inhibitory concentration of bromelain enzymes from peel and stem of pineapple was 1% with inhibition zones of 11.45 mm and 12.24 mm respectively. Bromelain enzymes from the peel and stem of pineapple provide the same activity in inhibiting the growth of Staphylococcus aureus bacteria. Keywords : Bromelain; minimum inhibition concentration; ammonium sulfate precipitation

PENYULUHAN DAN PELATIHAN POTENSI TANAMAN ZINGEBER OFFICINALE SEBAGAI PRODUK KESEHATAN INSTAN UNTUK MENGATASI ANTIFLAMASI Sagita, Desi; Rudheka, Afrudi; Pratiwi, Anis; A, Eva; M, Putri; WH, Hani; RS, Miftah; G, Sri; A, Widya
Martabe : Jurnal Pengabdian Kepada Masyarakat Vol 3, No 2 (2020): Martabe : Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jpm.v3i2.355-362

Traditionally people use ginger rhizome by boiling. To overcome these problems, and innovation made by making instant drinks with ginger rhizome raw materials. This community service is the application of the results of research conducted on ginger rhizomes in the form of powder, material from the plant's parts show activities that can relieve pain found in ginger rhizome extract. Community service carries out by utilizing the ginger rhizome to be used as a natural anti-inflammatory in the form of instant powder. The community service carries out by providing information and product-making demonstrations. Participants expected to increase knowledge about the benefits of the ginger rhizomes that are widely grown in the area, so that the community can utilize the ginger plant parts directly for use as an anti-inflammatory and can increase endurance in the form of instant powders. The results of the community service that carries out have enthusiastic people to take part in counseling and demonstrations on the production of instant ginger rhizome products.

Co-Authors A, Eva A, Widya Afrudi Rudheka Alfiana, Dessinta Anggresani, Lia Anis Pratiwi Ara Ofiana Armini Hadriyati Astridawati, Indah Barmi Hartesi Dahlia, Dimbi Dedi Eva A Fatimah Ria Sitompul Fhatia Medina Fitri, Kurnia Fiwi Juni Trisia G, Sri Hani WH HARTESI HARTESI HARTESI, HARTESI Hastuti Hastuti Helsa Raudatul Qalbi Hesty Murtiah Ibrahim, Puji Astuti Ichwani, Muhammad Nurul Iis mira anggraini Ilahi, Silvi Riska Imelda br. Siahaan Indah Astridawati Indri Meirista Julia Ambar Sari juliper nainggolan Kurniadi, Helman Lia Anggresani Lia Anggresani Lili Andriani Lili Andriani Lili Andriani Lili Loreza Sitanggang Linuria Linuria Linuria, Linuria Lufita, Lufita M, Putri Maya Gusri Yanti Medina, Fhatia Mery Safitri Michiko Mamesah Miftah RS Muhammad Nurul Ichwani Muhammad Randa Paska Harianty Novita Pratiwi, Anis Putri M Rahmadevi Randa, Muhammad Ratna Delfira Retty Filliani Rizky Ramdani Fajri Rizky Yulion Rozalti Rozalti RS, Miftah Rudheka, Afrudi Safitri, Mery Santi Perawati Santi Perawati Santi Perawati, Santi Sarah Christin Sari, Julia Ambar Septa Pratama Siti Hamidatul 'Aliyah Sri G Susanti Susanti Sutrisno , Deny Triana Wulansari Wahyu Perdaka WH, Hani Widya A Yuli Fitri Utami Yuliawati Yuliawati Yuni Andriani ‘Aliyah, Siti Hamidatul

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