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All Journal KOMUNITAS: INTERNATIONAL JOURNAL OF INDONESIAN SOCIETY AND CULTURE Local Wisdom : Jurnal Ilmiah Kajian Kearifan Lokal Jurnal Biologi Tropis Biosaintifika: Journal of Biology & Biology Education Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdi Insani BIOEDUPAT: Pattimura Journal of Biology and Learning Pakem : Jurnal Pengabdian Kepada Masyarakat Rumphius Pattimura Biological Journal Kalwedo Sains (KASA) Berita Biologi
Ferymon Mahulette, Ferymon
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Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Public Health Social Sciences Other

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Journal : Rumphius Pattimura Biological Journal

 1 
INTEGRATION OF PROJECT BASED LEARNING ETHNOSAINS TENUN IKAT TO IMPROVE UNDERSTANDING OF SCIENCE CONCEPTS AND CREATIVE THINKING STUDENTS JUNIOR HIGH SCHOOL (SMP) TANIMBAR Rupilele, Edy; Mahulette, Ferymon; Papilaya, Pamella Mercy
RUMPHIUS Vol 3 No 2 (2021): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv3i2p047-050

Abstract

This study aims to determine increased understanding of the concept of science through the integration of ethnoscience tenun ikat project based learning at SMP tanimbar, improving the ability to think creatively through the integration of the ethnoscience, differences in understanding the concept of science through the integration of project based learning ethnoscience, and differences in the ability to think creatively through the integration of ethnoscience. This research was conducted on April 26 – June 26 2021 located at SMPN 3 Wuarlabobar, SMPN 3 Waturu, SMP Christen Batuputih, SMPN 1 Atap Wermatang. The research design used a quasi-experimental design used two groups, namely the experimental group and the control group. The results of the study show that the integration of project based learning into the learning process can improve understanding of science concepts and ability to think creatively. The conclusion of this study is that there is a significant difference in the value of understanding science concepts and students' creative thinking ability between the sample groups of SMP Negeri 3 Wuarlabobar, SMP Negeri 3 Waturu, SMP Christen Batuputih and SMP 1 Atap Wertmatang.
ANTIBACTERIAL ACTIVITY OF BINAHONG ROOT EXTRACT (Anredera cordifolia (Ten.) Steenis) IN TREATMENT OF BOILS Pattiwael, Syane; Awan, Ali; Mahulette, Ferymon
RUMPHIUS Vol 4 No 1 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i1p018-025

Abstract

Infectious diseases caused by Staphylococcus aureus can be cured with traditional medicine, One example of a plant that has antibacterial power is binahong root. This study aims to determine the minimum inhibitory concentration (MIC) and minimum killing concentration (MBC) of binahong root extract against the growth of Staphylococcus aureus bacteria. Binahong root extract was obtained from maceration using absolute ethanol solvent. Furthermore, the extract obtained was made with a concentration dilution of 5%, 10%, 15% and 20%. The method used is disc diffusion. The results showed that each concentration of binahong root extract had an inhibitory effect on the growth of Staphylococcus aureus bacteria. This is influenced by the active compounds contained in the binahong root extract such as flavonoids, saponins, tannins, and alkaloids. Binahong root extract concentrations of 5%, 10%, 15%, and 20% were found to have zones of inhibition against the growth of S. aureus bacteria and the minimum inhibitory level (MIC) of Binahong root extract against the growth of S. aureus bacteria at a concentration of 5%.
EFFECTIVENESS OF ANTISEPTIC BASED ON GANDARIA LEAVES (Bouea macrophylla Griff) ON THE GROWTH OF Staphylococcus aureus AND Escherichia coli Lelapary, Claudia; Pattipeilohy, Mery; Mahulette, Ferymon
RUMPHIUS Vol 4 No 2 (2022): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv4i2p047-051

Abstract

Gandaria leaves (Boea macrophylla Griff) have potential as antibacterial. The ability to inhibit bacterial growth is inseparable from the role of active compounds, namely flavonoids, tannins, quinones and triterpenoids. Growth by microbes needs to be controlled, namely by inhibiting microorganisms, one way is by using chemicals, namely antiseptics. This study aims to determine the effectiveness of antiseptics made from gandaria leaves against the growth of S. aureus and E. coli bacteria. The concentrations of the ethanol extract of gandaria leaves used were 5%, 15% and 25% and betadine (+) and aquades (-) were used as controls. Antiseptic effectiveness test against S. aureus and E. coli bacteria using disc diffusion method. The test results showed that the antiseptic based on gandaria leaves had the ability to inhibit the growth of S. aureus with a concentration of 15% at 2.00 mm and 25% at 4.00 mm. Meanwhile, for E. coli bacteria, antiseptic made from gandaria leaves has an inhibition zone at a concentration of 15% at 1.00 mm. In accordance with the criteria for antiseptic power according to Davis Stout, gandaria leaves which are used as basic ingredients for antiseptics are included in the criteria for very weak antiseptic power (≤5). Therefore, the antiseptic based on gandaria leaves in this study was less effective in inhibiting the growth of S. aureus and E. coli bacteria.
Morphological Characteristics and Abundance of Bacteria in Fried Snack Foods from the Pujasera Canteen, Universitas Pattimura, and Fungi in Spoiled Fruits from Mardika Market, Ambon Pattipeilohy, Merry; Mahulette, Ferymon; Pelamonia, Alamanda
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal (in process)
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p039-046

Abstract

This study investigated the morphological characteristics and abundance of bacteria and fungi isolated from fried snack foods and spoiled fruits. Bacterial isolates were obtained from bakwan, batagor, and stuffed tofu, while fungal isolates were recovered from spoiled mango, banana, and papaya. Culture-based methods were employed to characterize macroscopic colony morphology and to quantify microbial abundance using the Total Plate Count (TPC) method. The results revealed distinct dominance patterns across different food types. In fried snacks, isolates BKK3, BTK2, and STK5 exhibited the highest TPC values, reaching up to 10⁷ CFU/g, indicating substantial post-processing bacterial contamination. In contrast, spoiled fruits were predominantly colonized by fungal isolates MGK3, BNK3, and PPK4, with TPC values also reaching 10⁷ CFU/g. These fungal isolates displayed typical filamentous mold characteristics, including white-to-green mycelia, granular to rugose textures, and radial and concentric growth patterns. This comparative analysis within a unified study framework demonstrates that difference in food type and intrinsic characteristics-namely ready-to-eat processed products with high environmental exposure versus fresh fruit tissues rich in nutrients and simple sugars-shape contrasting microbial dominance patterns. Fried snacks were primarily dominated by post-processing bacterial contaminants, whereas spoiled fruits were characterized by saprophytic fungal predominance. These findings highlight the critical role of physicochemical properties and handling conditions in determining microbial community dynamics and provide a conceptual basis for microbiological risk assessment in street-vended and fresh food systems. Practically, the results underscore the importance of hygiene control and post-processing management to mitigate microbial hazards and potential mycotoxin risks.
Co-Authors Antonius Suwanto Awan, Ali Dea Indriani Astuti Elly, Sovian Sesca Hajan, Esmi Irwina, Irwina Kalkoy, Yosualda Kosaplawan, Martafina Kurnia, Tri Santi Lelapary, Claudia Manusiwa, Gellian Gabriella Matulessy, Yoin M Melay, Stevin Mery Pattipeilohy, Mery Muskita, Christo Nikijuluw, Monica NISA RACHMANIA MUBARIK Papilaya, Pamella Mercy Pattiasina, Eifan B Pattipeilohy, Merry Pattiwael, Syane Pelamonia, Alamanda Ratusehaka, Nopri Rupilele, Edy Rupilu, Marselina R Rupilu, Marselina Ribka Rupilu, Ronny Sahetapy, Elsa Josma Sangur, Kristin Sarak, Brian Serpara, Ridolf Simal, Rufiati Smith, Alwi Tuapattinaya, Prelly Marsell Jolanda Warella, Juen Carla Welerubun, Inggrid WIDANARNI WIDANARNI