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All Journal Agrikultura Bioma : Jurnal Biologi Indonesia JMM (Jurnal Masyarakat Mandiri) Jurnal Agrotek Tropika Seminar Nasional Lahan Suboptimal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Ilmu Terapan Universitas Jambi Bioeksperimen
Weri Herlin
Universitas Sriwijaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Engineering Immunology & microbiology Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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Efektivitas Beberapa Metode Sterilisasi terhadap Tingkat Kontaminasi Eksplan Tulang Daun Duku pada Kultur In-Vitro Inaya, Rihani; Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Herlin, Weri; Negara, Zaidan P.
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Abstract

Inaya, R., Irmawati, I., Susilawati, S., Helen, H., Herlin, W., & Negara, Z. P. (2024). The effect of different sterilization techniques on the contamination levels of duku leaf midrib explant in In-Vitro Culture. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang 21 Oktober 2024. (pp. 667–678). Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).The Duku plant (Lansium domesticum) is one of the popular tropical fruit in indonesia. Using the idea of plant cells' totipotency, tissue culture is an alternate method for a quick and massive propagation of high-quality duku plant seedlings. This research was aimed to evaluate different sterilization materials and procedures on duku leaf midrib explants through tissue culture. The research was conducted from June to September 2024 at Tissue Culture Laboratory, Department  of  Agronomy, Universitas Sriwijaya (2°59’23.4”S 104°43’53.4”E). A combination of  sterilizing substances, including 70% alcohol, fungicide, bactericide, liquid detergent, and NaOCl, were utilized in this sterilization procedures. The percentage of live explants, browning explants, and explants infected with bacteria or fungi then were calculated using parametric descriptive analysis. The results showed that the P3 treatment's sterilant combination liquid detergent for 3 minutes, 0.2% of fungicide and bactericide for 10 minutes, 1% NaOCl for 15 minutes, and 70% alcohol for 5 minute produced the highest percentage of live explants (46%) with a relatively low percentage of contaminated explants (30%) and the lowest percentage of browning explants (23%). Thus P3 treatment was concluded as the best treatment to reduce the contamination level and browning of duku leaf midrib explant.
Optimalisasi Gulma Senduduk (Melastoma malabathricum L.) di sekitar tanaman Aren (Arenga pinnata) sebagai Pestisida Nabati pada Tanaman Cabai (Capsicum frutescens L.) Diadara, Dhiva Kinanti; Herlin, Weri; Mulya, Firman; Ramadani, Salwa Novia; Hasibuan, Thresia Firmaida; Ardianto, Febri; Pardede, M. Rabiul
Seminar Nasional Lahan Suboptimal Vol 12, No 1 (2024): Vol 12, No 1 (2024): Prosiding Seminar Nasional Lahan Suboptimal ke-12 “Revital
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

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Diadara, D. K., Herlin, W., Mulya, F., Ramadani, S. N., Hasibuan, T. F., Ardianto. F., & Pardede, M. R. (2024). Optimization of senduduk weed (Melastoma malabathricum L.) around aren (Arenga pinnata) plants as vegetable pesticide on chili plants (Capsicum frutescens L.). In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-12 Tahun 2024, Palembang 21 Oktober 2024. (pp. 186–194).  Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Cayenne pepper plants (Capsicum frutescens L.) were horticultural commodity plants that have high economic value in Indonesia which were never free from the problem of pests and diseases in cayenne pepper plants. The purpose of this research was to optimize weeds that grow around sugar palm (Arenga pinnata) plants. The method of using senduduk weed (Melastoma malabathricum L.) as a vegetable pesticide on chili plants (Capsicum frutescens L.) because was is aleolkimia content that has potential as a natural ingredient to control pests. This study tested senduduk extracts on chili plants to determine how effective they were in controlling pest attacks. The results showed that the use of senduduk weed extracts had a significant effect in reducing pest populations and increasing the productivity of chili plants. The conclusion that Optimizing senduduk weed around palm plants as a vegetable pesticide can be an environmentally friendly alternative to organic farming while helping to manage weeds that are often considered harmful.
OPTIMALISASI GULMA SEKITAR TANAMAN AREN SEBAGAI PESTISIDA NABATI RAMAH LINGKUNGAN UNTUK PERTANIAN BERKELANJUTAN Herlin, Weri; Herlinda, Siti; Irsan, Chandra; Pujiastuti, Yulia; Oktaviani, Oktaviani; Arinafril, Arinafril; Pratama, Rahmat; Susilawati, Susilawati; Sandi, Sofia
JMM (Jurnal Masyarakat Mandiri) Vol 9, No 3 (2025): Juni
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jmm.v9i3.31430

Abstract

Abstrak: Gulma sering dianggap sebagai tanaman pengganggu yang menurunkan produktivitas, namun gulma mengandung senyawa bioaktif seperti alkaloid, flavonoid, terpenoid, saponin, dan lainnya yang berpotensi dijadikan pestisida dan pupuk organik. Tujuan kegiatan pengabdian masyarakat ini adalah memberikan edukasi kepada petani di Desa Tanjung Dayang Utara, Sumatera Selatan, mengenai pemanfaatan gulma sebagai bahan dasar pestisida dan pupuk nabati yang ramah lingkungan serta berbiaya rendah. Metode pelaksanaan meliputi tahapan sosialisasi, penyuluhan, demonstrasi, pendampingan, dan evaluasi. Sebanyak 50 petani dan tokoh masyarakat mengikuti pelatihan pembuatan pestisida gulma dengan dua metode: sederhana dan ekstraksi. Evaluasi dilakukan secara berjenjang melalui diskusi pra-penyuluhan, uji praktek saat penyuluhan, dan pengamatan pertumbuhan tanaman. Hasil menunjukkan adanya peningkatan softskill peserta dalam pengolahan gulma hingga 75%, serta penurunan biaya pembelian pestisida dan pupuk sintetis hingga 40%. Kegiatan ini juga meningkatkan kesadaran petani terhadap praktek pertanian berkelanjutan dan penggunaan sumber daya lokal. Diharapkan ilmu yang diperoleh dapat ditransfer ke petani lain sehingga berdampak lebih luas dalam mendorong kemandirian dan efisiensi usaha tani.Abstract: Weeds are often seen as nuisance plants that reduce crop productivity. However, they contain bioactive compounds such as alkaloids, flavonoids, terpenoids, and saponins, which can be utilized as natural pesticides and organic fertilizers. This community service activity aimed to educate Tanjung Dayang Utara Village, South Sumatra farmers on using weeds as eco-friendly and low-cost agricultural inputs. The program involved socialization, counseling, presentations, mentoring, and evaluation. Fifty farmers and community leaders took part in training on pesticide production using simple and extraction methods. Evaluation was conducted through pre-counselling discussions, practical sessions, and plant growth observation. The activity improved participants' skills in weed processing by up to 75% and reduced the cost of synthetic pesticide and fertilizer use by up to 40%. It also increased awareness of sustainable farming practices and encouraged the use of local natural resources. The knowledge gained is expected to be shared with other farmers, fostering a broader impact through improved self-reliance and farming efficiency.
Integrating BSF larvae for the sustainable bioconversion of banana peels and sprout hulls waste Rizkawati, Vina; Andhini, Shabrina Lathiifah; Noer, Mohamad Isnin; Yudistira, Dwi Harya; Kurniati, Tri Handayani; Lisanti, Elsa; Komala, Ratna; Herlin, Weri
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.13637

Abstract

The management of organic waste presents a significant challenge in Indonesia, especially fruit and vegetable by-products. Black Soldier Fly (BSF, Hermetia illucens) larvae offer an effective solution due to their high potential for organic waste conversion. This study investigated the efficacy of BSF larvae in reducing and bioconverting organic waste composed of banana peels and sprout hulls, and examined the influence of EM4 fermentation on larval growth performance. Waste Reduction Index (WRI) and bioconversion rate (BCR) were calculated as percentages and analyzed descriptively, followed by the Kruskal–Wallis test. Furthermore, larval length and body weight were measured at 7, 11, 14, and 18 days of larval age and analyzed using Kruskal–Wallis with Dunn’s post-hoc test. The results showed that EM4–fermented substrates containing a 1:3 ratio of banana peels to sprout hulls showed the highest waste reduction and bioconversion potential among all mixture treatments (P < 0.05). Although larval length and weight were lower than control substrate, no significant difference in larval length was detected, while individual larval weight was significantly higher compared to other organic waste combinations (P < 0.001). In this study, EM4 fermentation enhanced lignocellulosic degradation, improving substrate digestibility for BSF larvae and optimizing bioconversion efficiency, thus supporting more sustainable organic waste management strategies.
OPTIMIZING SURFACE STERILIZATION OF Lansium domesticum LEAF LAMINA EXPLANTS USING SODIUM HYPOCHLORITE Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Inaya, Rihani; Herlin, Weri
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 10 No. 2 (2026): Volume 10, Nomor 2, April 2026
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v10i2.54331

Abstract

Lansium domesticum Corr. is a fruit crop with high consumer demand; however, its conventional propagation is relatively slow. In vitro culture offers a potential alternative to accelerate plant propagation. This study aimed to evaluate sodium hypochlorite (NaOCl)-based sterilization protocols and identify the most effective treatment for in vitro establishment of leaf lamina explants. The experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Sriwijaya, from July to September 2024 using five sterilization treatments with different NaOCl concentrations and exposure times. Data were analyzed descriptively to determine the percentages of bacterial contamination, fungal contamination, surviving explants, and browning incidence. The results showed that the treatment consisting of liquid detergent (3 min), 0.2% streptomycin sulfate (10 min), 0.2% benomyl (10 min), 1% NaOCl (15 min), and 70% ethanol (5 min) was the most effective protocol. This treatment resulted in 63% explant survival, 0% browning, 33% bacterial contamination, and 3% fungal contamination. This study provides a practical sterilization protocol that balances antimicrobial effectiveness and tissue viability, representing a novel contribution for in vitro propagation of Lansium domesticum. The findings have important implications for improving large-scale propagation efficiency of this economically important tropical fruit species. These results indicate that moderate NaOCl exposure is essential to achieve an optimal balance between contamination control and explant viability in in vitro culture systems.
Dual utilisation of Chromolaena odorata (L.) R.M.King & H.Rob. (Asteraceae) as bokashi fertiliser and botanical pesticide to enhance growth and yield of chilli (Capsicum annuum L.; Solanaceae) Dhiva Kinanti Diadara; Weri Herlin; Astuti Kurnianingsih
Bioma Vol. 22 No. 1 (2026): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/bioma.v22i1.67930

Abstract

The application of bokashi fertiliser and botanical pesticides offers a sustainable approach to improving crop productivity. This study evaluated the effectiveness of Chromolaena odorata (L.) R.M.King & H.Rob. (Asteraceae) as both a bokashi fertiliser and a botanical pesticide for enhancing the growth and yield of chilli (Capsicum annuum L.; Solanaceae). A randomised block design with two factors was employed. The first factor was bokashi fertiliser at two rates (200 and 600 g plant⁻¹), while the second factor was botanical pesticide at four concentrations (0, 150, 200, and 250 g L⁻¹). Observed variables included plant height, flowering time, fruiting time, average fruit weight, and fruit number. Bokashi fertiliser significantly affected chilli growth and yield, with 600 g plant⁻¹ producing the best results. Botanical pesticide application also showed significant effects, with 200 g L⁻¹ providing the most favourable performance across several parameters. Although no significant interaction was detected between the two treatments, the combination of 600 g plant⁻¹ bokashi fertiliser and 200 g L⁻¹ botanical pesticide produced the highest overall yield. These findings demonstrate the potential of C. odorata-based inputs for sustainable chilli cultivation.
Natural pest control diversity on Oryza sativa L. (Poaceae) managed in organic and conventional cultivation systems in Desa Tegal Sari, Ogan Komering Ulu Timur, South Sumatera Gilang Putra Bintang; Weri Herlin; Chandra Irsan; Oktaviani
Bioma Vol. 21 No. 2 (2025): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/bioma.v21i2.54756

Abstract

Natural controls, such as predators and parasitoids, play a crucial role in maintaining the stability of agricultural ecosystems through biological pest control mechanisms. On the rice agriculture systems, we propose to evaluate the diversity and abundance of natural controls in organic and conventional rice cultivation systems in Tegal Sari Village, Ogan Komering Ulu Timur Regency, South Sumatra. Observations were conducted using a systematic survey method employing various types of insect traps, with organism identification carried out to the level of type. This research is expected to contribute to both academic and practical domains. The results revealed the presence of 10 arthropod species from four orders, including Araneae, with dominant species such as Pardosa pseudoannulata, Tetragnatha extensa, Tetragnatha montana, Tetragnatha sp., Tigrosa annexa, and Zygiella are always higher on the organic rice agricultural system compared to the conventional system. The diversity analysis indicated that sweep nets were more effective in capturing natural controls compared to other traps, with a Shannon-Wiener diversity index value of 2.17, suggesting moderate diversity. These findings emphasize that organic farming supports biodiversity conservation and provides a scientific foundation for the development of pest management strategies.
Co-Authors Abu Umayah Abu Umayah Andhini, Shabrina Lathiifah Andini Siregar, Regina Sri Anggarini, Dewi Anita Setyawati Apriyani, Eka Ardianto, Febri ARINAFRIL ARINAFRIL Arka, Khoirunisya Arsi Arsi Arsi, Arsi Aryanti, Riri Mutiara Astuti Kurnianingsih Aulia Chandra, Fachdilla Ayu Wandhari, Vindha Az Zahra, Aisyah Dwi Bambang Gunawan Bambang Gunawan Bintang, Gilang Putra Chandra Irsan Chandra Irsan Cinta, Lara Destia, Heni Dhiva Kinanti Diadara Diadara, Dhiva Kinanti Elsa Lisanti Erise Anggraini, Erise Fatkha, Farah Nabila Gilang Putra Bintang Hakari, Irfan Mohandis Harman Hamidson Hasibuan, Thresia Firmaida Helen, Helen Inaya, Rihani Indah, Mawar Irmawati Irmawati Irmawati Irmawati Jesika, Jesika Maschamah, Rahmaningrum Meitha Sari, Dwi Mohamad Isnin Noer Mulya, Firman Negara, Zaidan P. Oktaviani Oktaviani Oktaviani oktaviani Pakpahan, Tukkot Pardede, M. Rabiul Prim Suardi Yudanto, Marcelinus Rido Putri, Jeni Angela Raffi, Muhammad Rahmat Pratama, Rahmat Ramadani, Salwa Novia Ratna Komala, Ratna Rizkawati, Vina Salsabila, Sabrina SHK, Suparman SITI HERLINDA Sofia Sandi SUPARMAN . Tri Handayani Kurniati Yudistira, Dwi Harya YULIA PUJIASTUTI YULIA PUJIASTUTI Yulistin, Eka Yuniva, Ester Akta Zagar, Zagar Zelianty, Shafa Olivia