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All Journal Jurnal Ilmu-Ilmu Pertanian Indonesia Bioma : Jurnal Biologi Indonesia JMM (Jurnal Masyarakat Mandiri) Jurnal Agrotek Tropika Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Seminar Nasional Lahan Suboptimal PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Ilmu Terapan Universitas Jambi Bioeksperimen
Weri Herlin
Universitas Sriwijaya
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Public Health Social Sciences Other

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OPTIMIZING SURFACE STERILIZATION OF Lansium domesticum LEAF LAMINA EXPLANTS USING SODIUM HYPOCHLORITE Irmawati, Irmawati; Susilawati, Susilawati; Helen, Helen; Inaya, Rihani; Herlin, Weri
Jurnal Ilmiah Ilmu Terapan Universitas Jambi Vol. 10 No. 2 (2026): Volume 10, Nomor 2, April 2026
Publisher : LPPM Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/jiituj.v10i2.54331

Abstract

Lansium domesticum Corr. is a fruit crop with high consumer demand; however, its conventional propagation is relatively slow. In vitro culture offers a potential alternative to accelerate plant propagation. This study aimed to evaluate sodium hypochlorite (NaOCl)-based sterilization protocols and identify the most effective treatment for in vitro establishment of leaf lamina explants. The experiment was conducted at the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Sriwijaya, from July to September 2024 using five sterilization treatments with different NaOCl concentrations and exposure times. Data were analyzed descriptively to determine the percentages of bacterial contamination, fungal contamination, surviving explants, and browning incidence. The results showed that the treatment consisting of liquid detergent (3 min), 0.2% streptomycin sulfate (10 min), 0.2% benomyl (10 min), 1% NaOCl (15 min), and 70% ethanol (5 min) was the most effective protocol. This treatment resulted in 63% explant survival, 0% browning, 33% bacterial contamination, and 3% fungal contamination. This study provides a practical sterilization protocol that balances antimicrobial effectiveness and tissue viability, representing a novel contribution for in vitro propagation of Lansium domesticum. The findings have important implications for improving large-scale propagation efficiency of this economically important tropical fruit species. These results indicate that moderate NaOCl exposure is essential to achieve an optimal balance between contamination control and explant viability in in vitro culture systems.
Utilization of antagonistic bacteria and fungi for inducing resistance in red chili and cayenne pepper in Tanjung Dayang Utara Herlin, Weri; Herlinda, Siti; Pujiastuti, Yulia; Palupi , Rizki; Oktaviani, Oktaviani
Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Vol. 10 No. 1 (2026)
Publisher : Universitas Ahmad Dahlan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12928/jpm.v10i1.13061

Abstract

Plant health problems caused by pests and diseases remain a significant challenge for farmers, especially in cultivating red curly chili and cayenne pepper. Conventional reliance on synthetic pesticides raises environmental and economic concerns. This community service program aimed to enhance farmers' knowledge in using biological agents as alternatives to synthetic pesticides to strengthen plant resistance. The program was carried out in Tanjung Dayang Utara Village, Ogan Ilir, through stages of preparation, implementation, evaluation, mentoring, and monitoring. Methods included lectures and demonstrations on using Pseudomonas fluorescens and Trichoderma sp. through seed soaking and soil or foliar application. A total of 50 farmers attended, receiving both theoretical and practical training. The evaluation was conducted in three phases: pre-, during, and post-activity. Previous approaches relied heavily on synthetic pesticides, while recent innovations emphasize ecological pest management using microbial antagonists. The outcomes showed a high level of enthusiasm and improved understanding among farmers regarding natural pest resistance mechanisms. Farmers were able to practice the propagation and application of beneficial microbes supported by educational materials. The program reduced farmers' dependency on chemical inputs and contributed to sustainable agricultural practices. This model using replicable in similar agroecosystems facing pest and disease issues. The main problem addressed was farmers’ limited knowledge and skills in utilizing biological control agents as environmentally friendly alternatives. The implementation consisted of preparation, training through lectures and demonstrations, field practice, and evaluation. The results indicated measurable improvements, including increased knowledge based on evaluation scores and reduced reliance on chemical pesticides
Co-Authors Abu Umayah Abu Umayah Andhini, Shabrina Lathiifah Ardianto, Febri ARINAFRIL ARINAFRIL Arka, Khoirunisya Arsi Arsi Aryanti, Riri Mutiara Bambang Gunawan Bambang Gunawan Bintang, Gilang Putra Chandra Irsan Chandra Irsan Destia, Heni Diadara, Dhiva Kinanti Elsa Lisanti Fatkha, Farah Nabila Gilang Putra Bintang Hakari, Irfan Mohandis Hasibuan, Thresia Firmaida Helen, Helen Inaya, Rihani Indah, Mawar Irmawati Irmawati Irmawati Irmawati Jesika, Jesika Mohamad Isnin Noer Mulya, Firman Negara, Zaidan P. Oktaviani Oktaviani Oktaviani oktaviani Pakpahan, Tukkot Palupi , Rizki Pardede, M. Rabiul Rahmat Pratama, Rahmat Ramadani, Salwa Novia Ratna Komala, Ratna Rizkawati, Vina Saputra, Muhamad Ridho SHK, Suparman SITI HERLINDA Sofia Sandi SUPARMAN . Susilawati, Susilawati Tri Handayani Kurniati Yudistira, Dwi Harya YULIA PUJIASTUTI YULIA PUJIASTUTI Yuniva, Ester Akta Zagar, Zagar Zelianty, Shafa Olivia