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All Journal Alchemy : Journal of Chemistry Current Biochemistry The Journal of Pure and Applied Chemistry Research JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Chimica et Natura Acta QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Kartika Kimia Journal of Science Innovare EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Abdimas Kartika Wijayakusuma International Journal of Ethno-Sciences and Education Research Helium: Journal of Science and Applied Chemistry Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Advances In Social Humanities Research Jurnal Pengabdian Masyarakat Inovatif Jurnal Pengabdian Masyarakat Inovatif

Siti Warnasih

Universitas Pakuan

Author-ID : 664444

Religion Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Social Sciences Transportation Other

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ISOLASI PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) DARI DARAH MANUSIA SEHAT DENGAN METODE SENTRIFUGASI GRADIEN FICOLL Siti Warnasih; W Yulia; I. M Artika; R. T. Sasmono
EKOLOGIA Vol 16, No 1 (2016): JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Peripheral blood sel mononuclear cell (PBMC) is a white blood cell that can be isolated using the gradient centrifugation method. Ficoll is a reactant which can be used in this method because it produce a high viability i.e. 97.1% 1.0%. This research aims to isolate PBMC from healthy human blood. PBMCs result is used for analysis of dengue virus immune response.Dengue viruses infect PBMC, so going on the secretion of cytokine which is one marker of severity of dengue disease. Expression of cytokines can be measured using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).Venous blood from healthy human is takenaseptically using anticoagulants heparin, then PBMC is isolated with ficoll gradient centrifugation method to produce buffy coat that contain PBMC. Viability of PBMC is calculated using hemositometer with the help of a microscope.Based on the results obtained by PBMC isolation per mL of venous blood that is 2, 08x106 up to 2, 61x106 with an average of 2, i.e. 31x106 withviabilityof 95-99%. These results indicate that this method is quite effective in isolating PBMC from healthy human blood.Keyword : blood, ficoll, centrifugasigradien, PBMCs

AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA Siti Warnasih; Diana Widiastuti; Uswatun Hasanah; Laksmi Ambarsari; Purwantiningsih Sugita
EKOLOGIA Vol 19, No 1 (2019): EKOLOGIA: JURNAL ILMIAH ILMU DASAR DAN LINGKUNGAN HIDUP
Publisher : Universitas Pakuan

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.

Characterization of Batik Industrial Wastewater in Bogor City Siti Kholisoh; Intan Wulandari; Ani Iryani; Sutanto Sutanto; Siti Warnasih
Helium: Journal of Science and Applied Chemistry Vol 2, No 1 (2022): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Wastewater from the batik industry in Bogor City is the most waste produced from the dyeing process. The batik industry produces waste with concentrated color due to the high concentration of color content from the dyeing process. This affects water bodies as a direct disposal site for waste. Batik waste also has the potential to contain heavy metal compounds derived from the composition of dyes. This will have an impact on the disruption of the balance in the environment around the industry. Therefore, it is necessary to characterize batik wastewater to determine the pollutant content. This research aims to characterize the batik industry wastewater in the city of Bogor by measuring the levels of COD, BOD, TSS, pH, temperature, total chromium content, total phenol content, and sulfide content according to the parameters P.16/MENLKH/SETJEN /KUM.1/4/2019. The results showed the characteristics of the wastewater from the batik industry in Bogor City with levels of COD in dyeing waste at 797 mg/L and total waste at 52463 mg/L, BOD levels in dyeing waste at 431 mg/L, and total waste at 238 mg/L. TSS in the dyeing waste is 706.5 mg/L and the total waste is 3679 mg/L, the temperature in the dyeing waste is 26.9 °C and the total waste is 26.3°C, pH in staining waste at 1.62 and total waste at 6.82; the total chromium content in the staining waste was 2.62 mg/L and the total waste was 940.2 mg/L, the total phenol content and sulfide content in the dyeing waste and total waste was not detected. The characteristics of batik waste are not by the established quality standards, so there is a need for processing before the waste is discharged into the environment.

IDENTIFICATION OF ANTIBACTERIAL COMPOUNDS FROM ENDOPHYTIC BACTERIAL EXTRACT OF GREEN GRASS CINCAU PLANT (PREMNA OBLONGIFOLIA MERR) Anna Muthia Soliha; Leny Heliawati; Siti Warnasih
Helium: Journal of Science and Applied Chemistry Vol 1, No 2 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Green grass jelly (Premna oblongifolia Merr) is a plant that belongs to the Verbenaceae family with the Premna genus. The genus Premna has about 200 species in the Verbenaceae family which are spread in tropical to subtropical areas such as Asia, Africa, and Australia. This plant has potential as antibacterial, antipyretic, anti-inflammatory, and antioxidant. This research was conducted to make an extract of endophytic bacteria obtained from green grass jelly plants by maceration method. The extract obtained was tested for its antibacterial activity by disc diffusion method. Extracts with the highest antibacterial activity were identified by FTIR and GCMS. The results showed that the methanol extract of endophytic bacteria of green grass jelly plants had broad spectrum antibacterial activity because it was able to inhibit the growth of Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Pseudomonas aeruginosa). The results of FTIR and GCMS showed that the active compounds contained in the endophytic bacteria of green grass jelly plants were thought to be Nerolidol compounds or 3,7,11-trimethyl-1,6,10-dokatrien-3-ol which are secondary metabolites of the sesquiterpene alcohol group and play a role in as an antibacterial.

Aktivitas Imunostimulan Ekstrak Metanol Biji Kurma (Phoenix dactylifera) secara In Silico Terhadap Reseptor GIF dan COX-2 serta Uji In Vitro melalui Proliferasi Sel Limfosit Mencit Siti Warnasih; Tamimah Shafwatul Ishlah; Dianda Namira Azzahra; Gita Syahputra
ALCHEMY:Journal of Chemistry Vol 10, No 2 (2022): ALCHEMY: Journal of Chemistry
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v10i2.14076

Dates (Phoenix dactylifera) is one of the plants that very useful, but the date palm seeds that become waste have not been widely used. Based on studies that are known to contain bioactive compounds that can be suspected as antioxidants, antimicrobials and anti-inflammatory. However, research on immunostimulants from dates, especially in silico and in vitro, has not been widely carried out. Therefore, this study aims to determine the potential of date seed bioactive compounds as immunostimulants through in silico against Human Glycosylation-Inhibiting Factor (1GIF) and Human Cyclooxygenase-2 (5IKR) receptors as well as in vitro through the proliferation of lymphocyte cells of male mice (Mus musculus) balb/c strains with the MTT Assay methods. The results of the in silico study showed that 5-O-Caffeoylshikimic Acid had the highest interaction and stability, Gibbs free energy with 1GIF and 5IKR receptors, -6.68 kcal/mol and -8.58 kcal/mol, respectively. This value is known to be higher than the Caffeic Acid as control ligand. The results of in vitro research obtained methanol extract of date seeds at a concentration series of 6.25; 12.5; 25 µg/mL had higher lymphocyte cell proliferation activity which was indicated by an increasing OD value, and at a concentration of 25 μg/mL produces the highest immunostimulant activity. Therefore, date seed extract containing the compound 5-O-Caffeoylshikimic Acid can potentially be an immunostimulant agent.

DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING SPECTRODIRECT Ade Heri Mulyati; Leny Heliawati; Sutanto .; Uswatun Hasanah; Diana Widiastuti; Siti Warnasih; Yulian Syahputri
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare, Volume 03 Number 02 2020
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3006

Increased human activities will cause various problems, one of which is water pollution in water sources because it receives a pollution load that exceeds its carrying capacity. In the context of environmental conservation, the government has set quality standards for liquid waste produced by various industries and other activities in a Decree of the State Minister for the Environment. COD (Chemical Oxygen Demand) is one of the most important parameters in wastewater quality standards. This study aims to validate the COD analysis method which refers to COD Thermoreactor System (LOVIBOND brand) at Pakuan University Service Laboratory, to ensure the validity of COD analysis results at Pakuan University Service Laboratory. Spectrodirect COD analysis method provides results that meet the requirements of accuracy, precision, LOD, and LOQ which is quite good. The results of the Coefficient of Variation give good results with a value of 1.8841% indicating the accuracy of the analysis method is good. The accuracy parameter also gives good results and meets the requirements with a% recovery value of 98.70351%. Overall, it can be stated that the spectrodirect analysis method tested is an accurate, precise, simple, and economical method for determining COD levels

PHYTOCHEMICAL CHARACTERIZATION AND TANNIN STABILITY TEST FROM KLUWEK (Pangium edule Reinw) Siti Warnasih; Uswatun Hasanah
Journal of Science Innovare Vol 1, No 2 (2018): Journal of Science Innovare, Volume 01 Number 02 2018
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v1i02.1000

One of the herbs that can be used as a source of natural dye is the kluwek which is brown. This study aims to determine the type of secondary metabolite compounds producing color, and to determine the stability of tannin in various conditions such as pH, oxidizing, UV rays, heating temperature, and storage using UV-Vis spectrophotometry method. This research includes preparation of kluwek, extraction with acetone:water (7:3) with maceration method, phytochemical test (tannin, flavonoid, carotenoid, and anthocyanin) and total tannins, and tannin stability test on various pH, oxidizing, UV rays, temperature and duration of heating, and storage using the UV-Vis spectrophotometry method. The results obtained are kluwek extract containing tannin, flavonoid, carotenoid, and anthocyanin compounds, and total tannins 2.80%. Based on the tannin content, kluwek extract stable at pH 6-7, heating 60-80 oC, and storage up to 9 days at temperature 27 °C, and kluwek extract unstable when contact with 1% H2O2, UV light, and if kept at cold temperature (10 ˚C).

DEVELOPMENT OF COD (CHEMICAL OXYGEN DEMAND) ANALYSIS METHOD IN WASTE WATER USING UV-VIS SPECTROPHOTOMETER Uswatun Hasanah; Ade Heri Mulyati; Sutanto .; Diana Widiastuti; Siti Warnasih; Yulian Syahputri; Tri Panji
Journal of Science Innovare Vol 3, No 2 (2020): Journal of Science Innovare, Volume 03 Number 02 2020
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/jsi.v3i2.3007

The COD (Chemical Oxygen Demand) analysis method based on the Indonesian National Standard (SNI) is a revision of SNI 06-6989.2-2004, Water and wastewater - Part 2: Method of testing for chemical oxygen demand (COD) with closed reflux spectrophotometrically. This SNI uses references from international standard methods, namely Standard Methods for the Examination of Water and Wastewater, 21st Edition, editor of LS Clesceri, AE Greenberg, AD Eaton, APHA, AWWA and WEF, Washington DC, 2005, Methods 5220 D (Closed Reflux, Colorimetric Methods). The purpose of this study was to validate the total method for testing chemical oxygen demand (COD) in water and wastewater with the reduction of Cr2O7 2- spectrophotometrically in the range of COD values of 100 mg / L to 900 mg / L measurements were made at a wavelength of 600 nm. and COD values less than or equal to 90 mg / L measurements were made at a wavelength of 420 nm. The results showed that the method SNI 06-6898.2- 2009 has good validation results including the r results obtained from the calibration curve equation of 0.998, a precision of 1.82%, an accuracy of 98.25% and LOD and LOQ of 12.27 mg / L and 25, 61 mg / L.

EKSTRAKSI ZAT WARNA DARI KLUWEK (Pangium edule Reinw) MENGGUNAKAN BERBAGAI PELARUT Siti Warnasih; Uswatun Hasanah
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 18, No 1 (2018): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v18i1.806

Natural brown color of kluwek is fermented from picung seeds that can be isolated to replace synthetic brown dyes. Natural brown color is come from the Tannin, in which can be obtained by the solvent extraction process. This study aims to determine the best solvent in extracting natural brown color from kluwek using the maceration method based on the maximum absorption of the extract produced. This research consisted of making of simplicia, measurement water content from fresh kluwek and dry simplicia, extraction using 4 types of solvents (water, ethanol, acetone, and hexane), phytochemical extraction, and identification using UV-Vis Spectrophotometer. The results showed that the yield of kluwek extract with water solvent was 19.42%, ethanol 19.68%, acetone 40,51%, and hexane 45,42% with the color of extract consecutively dark brown, brown, dark yellow, and light yellow. Positive results containing tannins and flavonoid are shown from extraction with water and ethanol, while acetone and hexane extracts were negative. All extracts produce absorption of maximum wavelength at 200-400 nm which containing tannin-rich compounds (proanthocyanidins tannin) or condensed tannins are tannin flavonoids.Key words: kluwek, maceration, tannin, dyes

ISOLASI PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) DARI DARAH MANUSIA SEHAT DENGAN METODE SENTRIFUGASI GRADIEN FICOLL Siti Warnasih; W Yulia; I. M Artika; R. T. Sasmono
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 16, No 1 (2016): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v16i1.979

Peripheral blood sel mononuclear cell (PBMC) is a white blood cell that can be isolated using the gradient centrifugation method. Ficoll is a reactant which can be used in this method because it produce a high viability i.e. 97.1% Â± 1.0%. This research aims to isolate PBMC from healthy human blood. PBMCâ€™s result is used for analysis of dengue virus immune response.Dengue viruses infect PBMC, so going on the secretion of cytokine which is one marker of severity of dengue disease. Expression of cytokines can be measured using quantitative real-time reverse transcription â€“ polymerase chain reaction (qRT-PCR).Venous blood from healthy human is takenaseptically using anticoagulants heparin, then PBMC is isolated with ficoll gradient centrifugation method to produce buffy coat that contain PBMC. Viability of PBMC is calculated using hemositometer with the help of a microscope.Based on the results obtained by PBMC isolation per mL of venous blood that is 2, 08x106 up to 2, 61x106 with an average of 2, i.e. 31x106 withviabilityof 95-99%. These results indicate that this method is quite effective in isolating PBMC from healthy human blood.Keyword : blood, ficoll, centrifugasigradien, PBMCs

Co-Authors Achmad, Dinar Munggaran Ade Heri Mulyati Ade Heri Mulyati, Ade Heri Agustine, Dine Alawiyah, Hanis Amelia, Nisa Anak Agung Istri Sri Wiadnyani Ani Iryani Anna Muthia Soliha Artika, I. M Deden Ardiansyah Dhiandani, Sri Boedi Diana Widiastuti Dianda Namira Azzahra Effendi, Herlinda Eka Herlina Elly Sukmanasa Eneng Tita Tosida Faliha Arinda Lestari Fredi Andria Gita Syahputra Gymnasti, Alista Dwis I. M Artika Indriyani, Silvia Intan Wulandari Irma Anggraeni Kholisoh, Siti LAKSMI AMBARSARI Leny Heliawati Linda Jati Kusumawardani Lisa Giovanny Marom, Muhamad Fathul Mastri Juniarto Novi Fajar Utami Nurmayani, Reni Nurul Marfira Prihastuti Harsani Purwantiningsih Sugita R. T. Sasmono Sabania, Ikrimah Nur Sadjarwa, Boedi Samsudin Samsudin Sasmono, R. T. Simalango, Siska Juliani Sinaga, Siska Elisahbet Siti Fatimah Siti Kholisoh Sofian, Yayan Sutanto Sutanto . Sutanto Sutanto Sutanto Sutanto Sutoyo, Muhammad Rayhan Tamimah Shafwatul Ishlah Tri Panji Triastinurmiatiningsih, Triastinurmiatiningsih Uswatun Hasanah Uswatun Hasanah USWATUN HASANAH W Yulia Yuli Wahyuni Yulia, W Yulian Syahputri Zahra, Mutiara Zuniar Subastian

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