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All Journal Alchemy : Journal of Chemistry Current Biochemistry The Journal of Pure and Applied Chemistry Research JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Chimica et Natura Acta QARDHUL HASAN: MEDIA PENGABDIAN KEPADA MASYARAKAT Jurnal Kartika Kimia Journal of Science Innovare EKOLOGIA : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Jurnal Abdimas Kartika Wijayakusuma Jurnal Sains dan Teknologi International Journal of Ethno-Sciences and Education Research Pharmaceutical Sciences and Research (PSR) Helium: Journal of Science and Applied Chemistry Jurnal Abdi Inovatif : Pengabdian kepada Masyarakat Advances In Social Humanities Research Jurnal Pengabdian Masyarakat Inovatif Jurnal Pengabdian Masyarakat Inovatif
Siti Warnasih
Universitas Pakuan
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Dentistry Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Transportation Veterinary Other

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EKSTRAKSI ZAT WARNA DARI KLUWEK (Pangium edule Reinw) MENGGUNAKAN BERBAGAI PELARUT Siti Warnasih; Uswatun Hasanah
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 18, No 1 (2018): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v18i1.806

Abstract

Natural brown color of kluwek is fermented from picung seeds that can be isolated to replace synthetic brown dyes. Natural brown color is come from the Tannin, in which can be obtained by the solvent extraction process. This study aims to determine the best solvent in extracting natural brown color from kluwek using the maceration method based on the maximum absorption of the extract produced. This research consisted of making of simplicia, measurement water content from fresh kluwek and dry simplicia, extraction using 4 types of solvents (water, ethanol, acetone, and hexane), phytochemical extraction, and identification using UV-Vis Spectrophotometer. The results showed that the yield of kluwek extract with water solvent was 19.42%, ethanol 19.68%, acetone 40,51%, and hexane 45,42% with the color of extract consecutively dark brown, brown, dark yellow, and light yellow. Positive results containing tannins and flavonoid are shown from extraction with water and ethanol, while acetone and hexane extracts were negative. All extracts produce absorption of maximum wavelength at 200-400 nm which containing tannin-rich compounds (proanthocyanidins tannin) or condensed tannins are tannin flavonoids.Key words: kluwek, maceration, tannin, dyes
ISOLASI PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMCS) DARI DARAH MANUSIA SEHAT DENGAN METODE SENTRIFUGASI GRADIEN FICOLL Siti Warnasih; W Yulia; I. M Artika; R. T. Sasmono
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 16, No 1 (2016): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v16i1.979

Abstract

Peripheral blood sel mononuclear cell (PBMC) is a white blood cell that can be isolated using the gradient centrifugation method. Ficoll is a reactant which can be used in this method because it produce a high viability i.e. 97.1% ± 1.0%. This research aims to isolate PBMC from healthy human blood. PBMC’s result is used for analysis of dengue virus immune response.Dengue viruses infect PBMC, so going on the secretion of cytokine which is one marker of severity of dengue disease. Expression of cytokines can be measured using quantitative real-time reverse transcription – polymerase chain reaction (qRT-PCR).Venous blood from healthy human is takenaseptically using anticoagulants heparin, then PBMC is isolated with ficoll gradient centrifugation method to produce buffy coat that contain PBMC. Viability of PBMC is calculated using hemositometer with the help of a microscope.Based on the results obtained by PBMC isolation per mL of venous blood that is 2, 08x106 up to 2, 61x106 with an average of 2, i.e. 31x106 withviabilityof 95-99%. These results indicate that this method is quite effective in isolating PBMC from healthy human blood.Keyword : blood, ficoll, centrifugasigradien, PBMCs
AKTIVITAS ANTIOKSIDAN DAN FLAVONOID EKSTRAK BIJI KURMA Siti Warnasih; Diana Widiastuti; Uswatun Hasanah; Laksmi Ambarsari; Purwantiningsih Sugita
Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup Vol 19, No 1 (2019): Ekologia : Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/ekol.v19i1.1660

Abstract

Date seeds are waste from palm fruit processing that has not been utilized optimally. Date seeds contain flavonoids which are known to act as antioxidants. The aim of this study was to determine the antioxidant activity and flavonoid of methanol extract which was carried out by soxhletation and its fractionation results. Date seeds are made into simplicia, soxhlet extraction with methanol, then the methanol extract is fractionated in stages by liquid-liquid partition with n-hexane, ethyl acetate, and n-butanol. Each fraction and extract was determined by its antioxidant activity with the DPPH method and its flavonoid was determined by spectrophotometry. Ethyl acetate fraction has the strongest antioxidant activity with the lowest IC50 value, that is equal to 5.74 ± 0.05μg/mL, followed by methanol extract of 9.55±0.53 μg/mL, n-butanol fraction 19.73±0,58 μg/mL, and n-hexane fraction of 289.59±10.52 μg/mL, while the IC50 value for vitamin C as a positive control was 4.29±0.74 μg/mL. The highest flavonoids were produced from ethyl acetate fractions which amounted to 1484.33 ± 161.47 mg Quercetin Equivalent (QE) / 100 g, followed respectively by methanol extract of 282.84±13.72 mg QE/100 g.
Comparison of COD Determination Methods FAS Titrimetric with UV-Vis Spectrophotometry Intan Wulandari; Uswatun Hasanah; Siti Warnasih
Helium: Journal of Science and Applied Chemistry Vol 3, No 1 (2023): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v3i1.8097

Abstract

Liquid waste is one of the factors causing contamination of the aquatic environment. One of the chemical parameters of water quality namely Chemical Oxygen Demand (COD). This research was conducted at three different concentration levels, namely low, medium, and high, with samples of river water, domestic wastewater, and sago liquid waste. The method used is UV-Vis spectrophotometry (SNI 6989.2: 2019) and FAS titrimetry (APHA, 2017 methods 5220 D); for the UV-Vis spectrophotometry method in the range 90 mg/L, the wavelength was measured at 420 nm, while for high levels in the range 100 mg/L x 900 mg/L, it was measured at 600 nm. The quality control parameters used are accuracy and precision parameters. The purpose of this study was to compare the COD determination between UV-vis spectrophotometry and FAS titrimetry and to determine the validity and correlation of the two methods—a comparison of the results of the two methods used in the F test. The results showed that the COD values from UV visible spectrophotometry and FAS titrimetry yielded good precision and accuracy values and met the acceptable limits, namely %RSD 10% and 90% accuracy %R 110%. However, the results of the COD analysis using the UV-Vis spectrophotometry method were lower by 0.8556 than the results of the COD analysis using the FAS titration method, with a correlation coefficient r2 = 0.982. The average concentration of UV-Vis spectrophotometry in samples of sago wastewater was 572.141 mg/L, domestic wastewater was 113.525 mg/L, and river water was 42.98 mg/L. The average COD level of the titrimetric method in sago wastewater was 641.888 mg/L, domestic wastewater was 219.251 mg/L, and river water was 58.016 mg/L. The results of the F test for these two methods produce an Fcount Ftable. The null hypothesis (Ho) is rejected, meaning there is a significant difference between the two methods.
Characterization of Physico-Chemical Levels of β-Carotene and Antioxidant Activity in Dried Carrot Dregs Powder Farida Nuraeni; Siti Warnasih; Vevi Hikmafitri Rizali
Helium: Journal of Science and Applied Chemistry Vol 1, No 1 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v1i1.2950

Abstract

Carrots contain many nutrients needed by the human body, especially as a source of vitamin A. Carrot pulp is a byproduct of the carrot juice process. The utilization of carrot dregs in household waste as food is still low. To increase added value, carrot dregs can be used as an additional ingredient as flour. The process of making dry carrot dregs uses the oven method with variations in drying temperature and time spent drying. The drying temperatures applied in this study were 40◦C, 50◦C, 60◦C and 70◦C with time variations of 17, 22, 27, 32 hours. Physicochemical testing includes yield levels, protein content test using the Kjeldahl method, water content test, ash content test, β-carotene content test using HPLC, vitamin A test, DPPH method antioxidant activity test and organoleptic test. Organoleptic tests in this study include texture, color and smell. The best results were obtained from the assessment of the organoleptic test which had the highest average value, namely carrot dregs dried at a temperature of 50 32C for 32 hours with a value of 6.0, which means the product was rather favored by the panelists.Carrot dregs dried at 50◦C for 32 hours had a yield rate of 19.30%, a moisture content of 4.41%, an ash content of 4.91%, a protein content of 4.07%, the antioxidant test had% inhibition of 661.30 ppm classified as a weak antioxidant. In the dried carrot dregs powder it still contains β-carotene although the level is only 1.92 mg with a vitamin level of 3187.2 IU. This shows that carrot dregs still contain β-carotene and vitamins even though the levels are not high and can function as an antioxidant inhibitor.
Identification of Antibacterial Compounds from Endophytic Bacterial Extract of Green Grass Cincau Plant (Premna oblongifolia Merr) Anna Muthia Soliha; Leny Heliawati; Siti Warnasih
Helium: Journal of Science and Applied Chemistry Vol 1, No 2 (2021): Helium: Journal of Science and Applied Chemistry
Publisher : Universitas Pakuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33751/helium.v1i2.4538

Abstract

Green grass jelly (Premna oblongifolia Merr) is a plant that belongs to the Verbenaceae family with the Premna genus. The genus Premna has about 200 species in the Verbenaceae family which are spread in tropical to subtropical areas such as Asia, Africa, and Australia. This plant has potential as antibacterial, antipyretic, anti-inflammatory, and antioxidant. This research was conducted to make an extract of endophytic bacteria obtained from green grass jelly plants by maceration method. The extract obtained was tested for its antibacterial activity by disc diffusion method. Extracts with the highest antibacterial activity were identified by FTIR and GCMS. The results showed that the methanol extract of endophytic bacteria of green grass jelly plants had broad spectrum antibacterial activity because it was able to inhibit the growth of Gram-positive bacteria (Bacillus subtilis) and Gram-negative bacteria (Pseudomonas aeruginosa). The results of FTIR and GCMS showed that the active compounds contained in the endophytic bacteria of green grass jelly plants were thought to be Nerolidol compounds or 3,7,11-trimethyl-1,6,10-dokatrien-3-ol which are secondary metabolites of the sesquiterpene alcohol group and play a role in as an antibacterial.
STRATEGI PEMASARAN ONLINE DAN INOVASI RASAN (RASA DAN KEMASAN) BAKSO CAH SOLO SEBAGAI UPAYA PENINGKATAN OMSET DI ERA NORMAL BARU Kusumawardani, Linda Jati; Siti Warnasih; Irma Anggraeni
Qardhul Hasan: Media Pengabdian kepada Masyarakat Vol. 10 No. 1 (2024): APRIL
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/qh.v10i1.4731

Abstract

Bakso Cah Solo (CS) merupakan salah satu UMKM (Usaha Mikro Kecil Menengah) yang terdampak secara ekonomi akibat wabah Covid-19. Kedai usaha yang berlokasi di Kecamatan Cileungsi, Kabupaten Bogor saat ini mengalani penurunan aktivitas jual beli akibat dampak penerapan PSBB pada seluruh lapisan masyarakat. Dalam kurun waktu enam bulan terakhir omsetnya menurun hingga 46,67% per bulan. Oleh karena itu, diperlukan strategi yang tepat untuk meningkatkan omset penjualan. Strategi akan difokuskan pada dua hal utama, yaitu pemasaran secara digital berbasis Less Contact Economy (LCE) dan inovasi RASAN (Rasa dan Kemasan). Strategi pemasaran dan distribusi produk dilakukan dengan cara pembuatan website, media sosial, dan pemanfaatan aplikasi ojek online yang diharapkan dapat meningkatkan jumlah konsumen melalui distribusi produk yang lebih luas dan tidak terbatas di daerah Cileungsi. Strategi kedua adalah melakukan inovasi berupa bakso dalam bentuk beku (frozen) dan menambah minimal empat varian rasa (keju, mozzarella, granat dan rusuk). Hal ini akan meningkatkan minat konsumen untuk mencoba produk-produk tersebut, sehingga target pasar akan meluas, konsumen meningkat sampai kalangan millennial. Berdasarkan strategi yang dilakukan, luaran kegiatan ini yaitu peningkatan kemampuan mitra dalam berjualan secara online, peningkatan jenis produk, peningkatan omset, dan peningkatan daya saing mitra melalui branding dan penjualan online.
Molecular Docking Studies of Phoenix dactylifera L. Against SARS-CoV-2 ACE-2 Receptor Warnasih, Siti; Mulyati, Ade Heri; Kholisoh, Siti
Jurnal Kartika Kimia Vol 5 No 2 (2022): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v5i2.177

Abstract

Dates (Phoenix dactylifera L.) are one of the most useful plants. According to previous research, those dates contain bioactive compounds with antioxidant, antimicrobial, and anti-inflammatory properties. Furthermore, dates can be used as an antiviral, but the potential of dates as an antiviral, particularly the SARS-CoV-2 antiviral, has received little attention. As a result, the goal of this study was to see if date palm bioactive compounds could be used as SARS-CoV-2 antivirals with molecular docking against the Angiotensin-Converting Enzyme-2 (ACE-2) receptor. The study was carried out using molecular docking methods with Autodock Vina, where 21 chemical compounds found in date palms as ligands were attached to the ACE-2 receptor, and four chemical compounds used as SARS-CoV-2 antiviral medicines, namely chloroquine, favipiravir, plitidepsin, and remdesivir, were used as a control ligand. The results showed that proanthocyanidin B1, B2, and quercetin ligands had the highest interaction and stability with Gibbs free energy values of -9.20 kcal/mol, -9.10 kcal/mol, and -8.20 kcal/mol, respectively. This value is known to be higher than control ligands plitidepsin -9.02 kcal/mol, remdesivir -7.88 kcal/mol, chloroquine -5.65 kcal/mol, and favipiravir -5.38 kcal/mol. As a result, dates can be used as a SARS-CoV-2 antiviral candidate.
Antioxidant Testing and Identification of Bioactive Compounds in Ethanol Extract of Propolis from Various Locations in Indonesia using LCMS-QTOF Mulyati, Ade Heri; Alawiyah, Hanis; Marom, Muhamad Fathul; Warnasih, Siti
Chimica et Natura Acta Vol 12, No 3 (2024)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v12.n3.53081

Abstract

Propolis produced by Trigona sp. stingless bees contains various bioactive substances including alkaloids, flavonoids, polyphenols, saponins, steroids, and terpenoids. The geographical origin of propolis production can influence its composition of secondary metabolites. This study aimed to qualitatively analyze ethanol extracts of propolis from Bintan, Lampung, and Makassar, and evaluate their phenolic and flavonoid contents, as well as antioxidant activities. The extraction method employed kinetic maceration with continuous stirring over 24 hours, using 70% ethanol as the solvent. Additionally, antioxidant activity was assessed using the DPPH method. The qualitative phytochemical analysis revealed that all three ethanol extracts contained alkaloids, flavonoids, and polyphenols. Saponin compounds were uniquely identified in the Makassar ethanol extract. Furthermore, antioxidant activity tests indicated significant potential in all three propolis extracts. These findings highlight the potential of Trigona sp. Stingless bee propolis as a valuable source of bioactive compounds beneficial for human health.
Penerapan Sistem Irigasi Tetes untuk Memaksimalkan Penggunaan Air bagi Petani Desa Kalong Liud Fatimah, Siti; Sutanto, Sutanto; Sutoyo, Muhammad Rayhan; Zahra, Mutiara; Mulyati, Ade Heri; Warnasih, Siti
Jurnal Abdimas Kartika Wijayakusuma Vol 5 No 3 (2024): Jurnal Abdimas Kartika Wijayakusuma
Publisher : LPPM Universitas Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jakw.v5i3.548

Abstract

Tim PPK Ormawa HIMASKA melakukan penerapan sistem irigasi tetes yang akan membantu memenuhi kebutuhan air tanaman selama musim kemarau dengan menjaga penggunaan air secara efisien di Desa Kalong Liud di mana sistem ini akan meningkatkan pemanfaatan unsur hara tanah, mengurangi tekanan air terhadap tanah, mempercepat adaptasi bibit, dan meningkatkan keberhasilan tumbuh tanaman. Selain itu, sistem irigasi tetes dapat mencapai tingkat efisiensi penggunaan air yang lebih tinggi. Metode yang dilakukan dalam kegiatan ini meliputi survei, pembentukan kelompok masyarakat, sosialisasi serta edukasi, pelaksanaan, dan evaluasi proses irigasi. Air dari toren SPAH dengan volume 1000 liter sebelum dimanfaatkan untuk irigasi tetes hanya dapat dipakai untuk tiga kali penyiraman, tetapi setelah menggunakan sistem irigasi tetes air dari SPAH dengan volume 1000 liter tersebut dapat dipakai untuk lima sampai enam kali penyiraman. Dengan begitu, dapat disimpulkan bahwa sistem ini sangat layak untuk diterapkan karena menekan penggunaan air untuk kebutuhan irigasi sehingga dapat menjadi solusi bagi para petani pada saat musim kemarau.
Co-Authors Achmad, Dinar Munggaran Ade Heri Mulyati Ade Heri Mulyati Ade Heri Mulyati, Ade Heri Agustine, Dine Alawiyah, Hanis Alista Dwis Gymnasti Amelia, Nisa Anak Agung Istri Sri Wiadnyani Anna Muthia Soliha Artika, I. M Deden Ardiansyah Dhiandani, Sri Boedi Diana Widiastuti Diana Widiastuti Dianda Namira Azzahra Dinar Munggaran Achmad Effendi, Herlinda Eka Herlina Eka Herlina Elly Sukmanasa Eneng Tita Tosida Faliha Arinda Lestari Farid Ramadhan Farida Nuraeni Fredi Andria Gita Syahputra Gymnasti, Alista Dwis I. M Artika Ikrimah Nur Sabania Intan Wulandari Irma Anggraeni Iryani, Ani Junita Darmawarni Kholisoh, Siti LAKSMI AMBARSARI Leny Heliawati Linda Jati Kusumawardani Lisa Giovanny Marom, Muhamad Fathul Mastri Juniarto Novi Fajar Utami Nurmayani, Reni Nurul Marfira Prakoso, Muhamad Iqbal Prihastuti Harsani Purwantiningsih Sugita R. T. Sasmono Sadjarwa, Boedi Samsudin Samsudin Sasmono, R. T. Silvia Indriyani Sinaga, Siska Elisahbet Siska Juliani Simalango Siti Fatimah Siti Kholisoh Sofian, Yayan Susanto Susanto Sutanto Sutanto . Sutanto Sutanto Sutanto Sutanto Sutanto Sutanto Sutoyo, Muhammad Rayhan Tamimah Shafwatul Ishlah Tri Panji Triastinurmiatiningsih, Triastinurmiatiningsih Uswatun Hasanah Uswatun Hasanah USWATUN HASANAH Vevi Hikmafitri Rizali W Yulia Yuli Wahyuni Yuli Wahyuni Yulia, W Yulian Syahputri Zahra, Mutiara Zuniar Subastian