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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Buletin Agrohorti Jurnal Fitopatologi Indonesia Jurnal Teknologi Dan Industri Pangan Jurnal Hortikultura Indonesia (JHI) BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal Hortikultura Jurnal AgroBiogen Jurnal Penelitian Tanaman Industri Buletin Tanaman Tembakau, Serat & Minyak Industri AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA International Journal of Applied Biology Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Penelitian Pertanian Tanaman Pangan Jurnal Geografi, Edukasi dan Lingkungan ANNALES BOGORIENSES Jurnal Agrotek Tropika Journal of Tropical Crop Science Warta Penelitian Perhubungan Menara Perkebunan JURNAL BUDIDAYA PERTANIAN Jurnal Penelitian Tanaman Industri (Littri) Buletin Tanaman Tembakau, Serat & Minyak Industri Perbal : Jurnal Pertanian Berkelanjutan Warta Penelitian Perhubungan Annales Bogorienses
Efendi, Darda
Agronomy And Horticulture Department, Faculty Of Agriculture, Bogor Agricultural University Jl. Meranti, IPB Darmaga Campus, Bogor 16680, Indonesia
Agriculture, Biological Sciences & Forestry Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Social Sciences Transportation Veterinary

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Uji Efikasi Teknik Kultur Meristem dan Kemoterapi untuk Eliminasi Sugarcane Streak Mosaic Virus (SCSMV) pada Tebu Ika Roostika; Sedyo Harsono; Darda Efendi; Deden Sukmadjaja; Cece Suhara
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 8, No 2 (2016): Oktober 2016
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/btsm.v8n2.2016.55-64

Abstract

Penggunaan benih bebas virus merupakan salah satu cara pengendalian penyakit virus. Jaringan tanaman dapat dibebaskan dari virus melalui aplikasi teknik eliminasi virus, seperti termoterapi, kemoterapi, kultur meristem, dan krioterapi. Tujuan penelitian ini adalah untuk menguji respon varietas tebu terhadap perlakuan teknik kultur meristem dan kemoterapi dengan bahan antiviral, serta untuk mengetahui efektivitasnya dalam mengeliminasi virus sugarcane streak mosaic virus (SCSMV) pada tebu. Penelitian ini dilakukan pada April−November 2015 di Laboratorium Kultur Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian dan Laboratorium Virologi, Fakultas Pertanian, Universitas Gadjah Mada. Penelitian terdiri atas tiga tahap, yaitu 1) Deteksi virus dari tanaman induk, 2) Aplikasi teknik kultur meristem dan kemoterapi, serta 3) Indeksing virus. Bahan tanaman yang digunakan adalah sebelas varietas tebu (GMP3, PS865, dan Kentung asal Bogor, PS862 dan Cening asal Cirebon, PS881 asal Jember, PSJK922 asal Malang, serta PS864, PS881, PSJK922, PSJT941 asal Pati). Deteksi virus dilakukan secara RT-PCR dengan primer universal MJ dan primer spesifik SCSMV. Bahan antiviral yang digunakan untuk kemoterapi adalah Ribavirin (0 dan 25 µg/l). Hasil uji RT-PCR menggunakan primer universal MJ menunjukkan bahwa empat varietas (GMP3 asal Bogor, PS864 dan PSJT941 asal Pati, serta Cening asal Cirebon) terinfeksi oleh Potyvirus. Empat varietas lainnya (PS862 asal Cirebon, PS881 asal Jember, PSJK922 asal Malang, dan Kentung asal Bogor) terbukti terserang virus SCSMV berdasarkan uji RT-PCR dengan primer spesifik. Seluruh meristem mampu beregenerasi membentuk tunas. Penggunaan Ribavirin 25 µg/l tidak menyebabkan penurunan daya tumbuh meristem (50−100%), bahkan seluruh varietas mampu bermultiplikasi tunasnya dibandingkan dengan kontrol yang hanya memiliki daya tumbuh 0−100%, dan tidak semua varietas mampu bermultiplikasi tunasnya. Secara tunggal, aplikasi teknik kultur meristem tidak mampu mengeliminasi virus SCSMV, namun jika dikombinasikan dengan perlakuan kemoterapi maka virus SCSMV dapat tereliminasi dengan efikasi sebesar 44,4%. The use of virus-free seedling is an option for controlling viral disease that can be obtained through the application of viral elimination method. Plant tissues can be eliminated from virus infection by applying virus thermotherapy, chemotherapy, meristem culture, and cryotherapy. The research objectives were to examine the response of sugarcane varieties to meristem culture treatments and antiviral agent and also to determine the efficacy rate of both techniques in eliminating SCSMV disease. The study was conducted atTissuseCulture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research andDevelpoment, and also at Virology Laboratory, Faculty of Agriculture, Gadjah Mada University.   This study consisted of three activities: 1) Virus detection of the mother plants, 2) Application of meristem culture and chemotherapy, and 3) Virus indexing. The plant material used was eleven varieties of sugarcane (GMP3, PS865, and Kentung from Bogor, PS862 and Cening from Cirebon, PS881 from Jember, Malang PSJK922 origin, as well as the PS864, PS881, PSJK922, PSJT941 from Pati). Virus detection was performed by RT-PCR assay with universal primer of MJ and specific primers of SCSMV. Antiviral used for chemotherapy was Ribavirin (0 and 25 µg/l). The result of RT-PCR using universal primers MJ showed that four varieties (GMP3 from Bogor, PS864 and PSJT941 from Pati, and Cening from Cirebon) were infected by Potyvirus. Based on RT-PCR assay with specific primer, four other varieties (PS862 from Cirebon, PS881 from Jember, PSJK922 from Malang, and Kentung from Bogor) were infected by SCSMV. All of meristems were able to regenerate to form shoots. The use of Ribavirin (25µg/l) did not decrease the growth rate of meristems and the shoots of all of the varieties could be multipied compared to control where the shoots could not be multiplied in all varietis. The application of meristem culture technique was not able to eliminate the SCSMV, but when it was combined with chemotherapy treatment, the SCSMV virus could be eliminated with the efficacy rate of 44.4%. 
EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS Yosi Zendra Joni; Riry Prihatini; Darda Efendi; Ika Roostika
Indonesian Journal of Agricultural Science Vol 17, No 1 (2016): April 2016
Publisher : Indonesian Agency for Agricultural Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/ijas.v17n1.2016.p9-16

Abstract

Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.
Morfogenesis Eksplan Keping Biji dari Tiga Klon Manggis (Garcinia mangostana L.) Pada Tiga Jenis Media Dasar Yosi Zendra Joni; Darda Efendi; Ika Roostika Tambunan
Jurnal Hortikultura Vol 24, No 2 (2014): Juni 2014
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v24n2.2014.p94-101

Abstract

Sistem regenerasi manggis secara in vitro merupakan metode alternatif yang mendukung upaya produksi benih secara masal dan pemuliaan tanaman manggis secara bioteknologi. Dalam kultur in vitro, jenis klon dan media dasar sangat menentukan pertumbuhan biakan manggis. Penelitian ini bertujuan untuk mempelajari respons in vitro (morfogenesis) tiga klon manggis yang dikulturkan pada tiga jenis media dasar. Penelitian dilaksanakan di Laboratorium Kultur Jaringan, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian (BB Biogen) dari bulan Januari sampai Agustus 2013. Percobaan disusun secara faktorial dalam lingkungan rancangan acak lengkap. Faktor pertama yaitu eksplan yang berasal dari tiga klon manggis (Leuwiliang, Wanayasa, dan Puspahiang). Faktor kedua adalah tiga jenis media dasar (MS, WPM, dan B5). Setiap media diperkaya dengan gula 30 g/l, phytagel 2,5 g/l, glutamin 300 mg/l, dan 6-benzyldenine (BA) 5 mg/l. Setiap perlakuan terdiri atas 25 ulangan (botol). Dalam setiap botol terdapat tiga irisan melintang biji manggis yang berasal dari satu biji. Hasil penelitian menunjukkan bahwa tidak terdapat interaksi antara jenis klon dengan jenis media dasar untuk peubah jumlah tunas, tinggi tanaman, jumlah daun/tunas, dan jumlah daun total. Terdapat interaksi antara jenis klon dengan jenis media dasar, yaitu untuk peubah jumlah nodul. Menariknya, penggunaan media MS menyebabkan jumlah tunas, jumlah daun total, dan jumlah nodul yang terbanyak. Tinggi tunas tertinggi diperoleh dari penggunaan media WPM dan B5. Selain itu, morfogenesis klon Wanayasa dan Puspahiang lebih baik daripada klon Leuwiliang
Kriopreservasi Tunas in Vitro Pepaya ‘Sukma’ Dengan Perlakuan Prakultur, Loading, Dan Dehidrasi Dengan Pvs2 Dan Modifikasinya Fitri Fatma Wardani; Joko Ridho Witono; Darda Efendi; Diny Dinarti
BERITA BIOLOGI Vol 20, No 2 (2021): Berita Biologi
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v20i2.3999

Abstract

Papaya has high genetic variability because it is an open-pollinated plant and has genotype and phenotypeare that are easily changed due to environment changes. Cryopreservation is a storing method of germplasm in liquid nitrogen (-196 oC) which can maintain the genotype and phenotype of germplasm. The experiment aimed to obtain the best preculture, loading, and dehydration for cryopreservation of papaya ‘Sukma’ in vitro shoots. For preculture, we planted shoots on MS media with 0.3 M and 0.4 M sucrose for 1, 2, and 3 days. In the loading treatment, we immersed shoots in loading solution (liquid MS+1.2M glycerol+0.4M sucrose) for 0, 10, 20, and 30 minutes. For dehydration, we immersed shoots in cryoprotectant (PVS2 and its modification) for 5, 10, and 15 minutes. Then, shoots were immersed in liquid nitrogen. The results showed thatshoots had the best survival rate while they had been precultured on MS medium with 0.3 M sucrose for 3 days. The best loading treatment time was 20–30 minutes. The best dehydration treatment was obtained by modification of PVS2 for 10 minutes. The shoots have not been able to recovery after cryopreservation, so it can be concluded that cryopreservation of in vitro papaya ‘Sukma’ shoots has not been successful.
The Analysis of β-cryptoxanthin and Zeaxanthin using HPLC in the Accumulation of Orange Color on Lowland Citrus Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi; Andria Agusta; Sri Yuliani
International Journal of Applied Biology Vol. 1 No. 2 (2017): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v1i2.3066

Abstract

Citrus peel color is one of the main quality attributes which was caused by the accumulation of carotenoids and its derivatives, especially β-citraurine. It makes citrus peel color looks attractive (orange). The orange color is a mixture of β-cryptoxanthin with β-citraurin. The objectives of this study were (1) to observe the effect of precooling and duration of proper ethylene exposure in the formation of orange color on citrus peel, (2) to identify and determine the β-cryptoxanthin content and total chlorophyll on citrus peel. Citrus was from Tuban, East Java while the study was conducted at PKHT IPB and LIPI. Precooling and without precooling treatment prior to injection of 100 ppm of ethylene exposed at 15 °C, duration of exposure control (0), 24, and 48 hours. The results show that the best color of the Citrus Color Index (CCI) is the precooling treatment and the duration of ethylene exposure for 24 hours, which can reduce total chlorophyll content about 8 times and proved to increase β-cryptoxanthin pigment content five times in accelerating the formation of orange citrus reticulata peel color to bright orange. Degreening has no significant effect on total dissolved solids and the firmness level of citrus fruits.Keyword: β-cryptoxhantin; citrus; chlorophyll; degreening; ethylene zeaxanthin.
Study of Several Stages of Maturity and Storage Temperature on Color Changes and Shelf Life of Mangosteen (Garcinia mangostana L.) Inanpi Hidayati Sumiasih; Roedhy Poerwanto; Darda Efendi
International Journal of Applied Biology Vol. 3 No. 1 (2019): International Journal of Applied Biology
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/ijab.v3i1.5967

Abstract

Color and freshness of mangosteen are important characters as benchmarks for consumers in the selection and purchase of mangosteen in the market. Color, freshness and shelf life of mangosteen are affected by the stage of maturity at harvest and the correct storage temperature. Information about the correct maturity stage for harvesting and storage temperature of mangosteen are needed by the mangosteen farmers, local merchant, and exporters as an effort to maintain the quality of fresh product. The objective of this research was to study the effect of several maturity stage at harvest and storage temperature to mangosteen color changes and shelf life during storage. The research used Completely Randomized Design of two factors. The first factor was the fruit maturity stage at harvest consisting of: Maturity Stage 1, 2, 3 and 4. The second factor was storage temperature of 15 oC and room temperature. The result of harvesting mangosteen at Maturity Stage 1 could maintain skin color longer than at Maturity Stage 2, 3, and 4. Mangosteens that were harvested at Maturity Stage 1 and 2, combined with storage temperature of 15 oC could maintain fruit quality up to 30 days after harvest and could be used for export market. While harvesting at Maturity Stage 3 could maintain fruit quality up to 25 days after harvest and Maturity Stage 4 up to 20 days after harvest. Harvesting at Maturity Stage 4 followed by 15 oC storage temperature and all Maturity stages combined with room temperature storage could be used for local market.Keyword: fruit color; horticultural commodities; queen of tropical fruits; shelf life 
STUDY ON FRUIT QUALITY OF SELECTED SEEDED PUMMELO CULTIVARS AND ITS RELATIONSHIP WITH ANTIOXIDANT ACTIVITY CONTENT DURING STORAGE PERIOD Wahyu Fikrinda; Slamet Susanto; Darda Efendi; Maya Melati
AGRIVITA, Journal of Agricultural Science Vol 37, No 3 (2015): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v37i3.519

Abstract

Indonesia has number of accessions and cultivars of pummelo which are prospective to be developed. Pummelo contains higher antioxidants thus beneficial for health. This research aimed to get information of physical and chemical quality differences, antioxidant capacity, and explain the relationship between fruit quality and antioxidant capacity of selected seeded pummelo cultivars. Fruit was harvested in Banyuwangi and Magetan while fruit quality assesment was conducted in Laboratory of Agronomy and Horticulture Depart-ment, IPB. The results showed that physical qualities of fruit (weight loss, peel softness and peel color) and chemical qualities (total soluble solids and total titratable acidity) were changed during storage. Adas Nambangan and Banyu-wangi cultivars have better physical and chemi-cal qualities than other cultivars during storage until 10 weeks after harvest because of good visual appearance, the lowest decreased in weight loss and the good ratio of TSS:TTA. Seeded pummelo cultivars with dark red to reddish white fruit pulp had significant higher total phenolic, carotenoid, anthocyanin and anti-oxidant capacity than white fruit pulp. There were negative correlations between antioxidant capacity with colored pulp and total phenolic content. Banyuwangi had the highest antioxidant capacity in the pulp, followed by Bali Merah, Adas Nambangan, Pamelo Magetan, Srinyonya, Bali Putih cultivars. 
KOMBINASI TERMOTERAPI DAN KHEMOTERAPI DENGAN KULTUR APEKS DAN MERISTEM UNTUK ELIMINASI VIRUS MOSAIK PADA TEBU / The Combined Treatment of Thermotherapy and Chemotherapy with Apex and Meristem Culture for Mosaic Virus Elimination in Sugarcane Ika Roostika; SEDYO HARTONO; DARDA EFENDI
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.19-28

Abstract

There are several ways to eliminate virus, suach as the application of thermoterapi and chemotherapy technique, and also the apex and meristem culture. One way to control this disease is the use of virus-free seedlings. The objective of this study was to find out the effect of combined treatment between thermotherapy or chemotherapy with apex or meristem culture to mosaic virus elimination of sugarcane. There were four steps in this research: (1) Virus detection of mother plant, (2) Application of thermotherapy at 50oC and chemotherapy by using Ribavirin 25 µg/l combined with apex culture, (3) Application of thermotherapy and chemotherapy combined with meristem culture, and (4) Evaluation of virus elimination. The plant materials used were PS862 from Cirebon (PS862-Crb), PS881 from Surabaya (PS881-Sby) and PSJK922 (PSJK922-Bgr) from Bogor. Virus detection was conducted by TEM and RT-PCR analysis. The temperature for thermotherapy was 500C and the antiviral agent was Ribavirin (0 and 25 μg/l). The result showed that thermotherapy or chemotherapy combined with apex culture could not eliminate virus infection. The combined treatment of chemotherapy and meristem culture could eliminate SCSMV in variety PS862-Crb based on RT-PCR assay, however TEM analysis still detected the viral particle. It was suggessted to udertake virus indexing of large number of samples to see the rate of virus elimination.Keywords: Saccharum officinarum L., Ribavirin, Potyvirus, TEM, RT- PCR
DEHIDRASI DAN PEMBEKUAN JARINGAN APEKS TEBU UNTUK PENYIMPANAN JANGKA PANJANG IKA ROOSTIKA; RARA PUSPITA DEWI LIMA WATI; DARDA EFENDI
Jurnal Penelitian Tanaman Industri Vol 21, No 1 (2015): Maret 2015
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v21n1.2015.25-32

Abstract

ABSTRAKTebu (Saccharum officinarum L.) merupakan tanaman yangdiperbanyak secara vegetatif. Kriopreservasi merupakan metode yangpaling sesuai untuk penyimpanan jangka panjang bagi tanaman yangdiperbanyak secara vegetatif. Dehidrasi dan pembekuan jaringan merupa-kan tahapan paling kritis yang menentukan keberhasilan kriopreservasi.Tujuan penelitian adalah untuk memperoleh durasi dehidrasi yang optimaldan metode pembekuan jaringan apeks tebu. Penelitian dilakukan diLaboratorium Kultur Jaringan, Kelompok Peneliti Biologi Sel danJaringan, Balai Penelitian dan Pengembangan Bioteknologi dan Sumber-daya Genetik Pertanian, Bogor pada Mei 2013 sampai Februari 2014.Untuk optimasi metode dehidrasi, apeks direndam dalam larutan PVS2(MS + gliserol 30% + etilen glikol 15% + dimetil sulfoksida 15% +sukrosa 0,4 M) selama 10, 20, 30, dan 40 menit. Untuk optimasi metodepembekuan, diujikan kombinasi perlakuan prakultur (dengan sukrosa 0;0,1; dan 0,3 M selama 5 hari) dan pemuatan dalam larutan LS (MS +gliserol 2 M + sukrosa 0,4 M) selama 0, 10, 20, dan 30 menit sebelumtahapan dehidrasi dan pembekuan jaringan di dalam nitrogen cair (-196 o C). Hasil penelitian menunjukkan durasi dehidrasi jaringan yangterbaik adalah 30 menit dalam larutan PVS2. Kombinasi perlakuanprakultur dengan sukrosa 0,3 M dan pemuatan dengan larutan LS selama10 menit merupakan metode terbaik untuk pembekuan jaringan. Persentasetumbuh sebelum dan setelah pembekuan dalam nitrogen cair berturut-turutadalah 100 dan 40%. Setelah kriopreservasi, biakan mampu tumbuhdengan tingkat multiplikasi tunas sekitar 10 tunas/eksplan. Metode yangdiperoleh pada penelitian ini berpeluang diterapkan untuk penyimpananplasma nutfah tebu dalam jangka panjang secara kriopreservasi daneliminasi patogen obligat secara krioterapi.Kata kunci: Saccharum officinarum L., apeks, dehidrasi, pembekuan,nitrogen cairABSTRACTSugarcane (Saccharum officinarum L.) is vegetatively propagatedplant. Cryopreservation is the most suitable method for long-termpreservation of vegetatively propagated plant. Dehydration and freezingare critical steps of successful cryopreservation so that it should beoptimized. The research aimed to obtain the optimal duration ofdehydration and freezing method of sugarcane apex tissues. Theexperiments were conducted at Tissue Culture Laboratory, Plant CellTissue Biology Group, Indonesian Center for Agricultural Biotechnologyand  Genetic  Resources  Research  and  Development  on  May2013−February 2014. To optimize dehydration method, the tissues wereexposured in PVS2 solution (MS + 30% glycerol + 15% ethylene glycol +15% dimethyl sulphoxide + 0.4 M sucrose) for 10, 20, 30, and 40 minutes.To optimize freezing method, the combined treatment of preculture withsucrose (0, 0.1, dan 0.3 M) for 5 days and loading in LS solution (MS + 2M glycerol + 0.4 M sucrose) for 0, 10, 20, dan 30 minutes) were testedbefore dehydration for 30 minutes and freezing in liquid nitrogen (-196 o C).The best duration of dehydration was 30 minutes. The combined treatmentof preculture on 0.3 M sucrose and loading for 10 minutes was the bestmethod for tissues freezing. Percentage of regrowth before and afterfreezing in liquid nitrogen was 100 and 40% respectively. Aftercryopreservation, the cultures could grow with high shoot multiplicationrate about 10 shoots/explant. The method resulted in this study can beapplied for long-term storage of sugarcane germplasms by cryopreser-vation and (elimination of obligate pathogens by cryotherapy.Keywords: Saccharum officinarum L., apex, dehydration, freezing, liquidnitrogen.
ANALYSIS OF GENETIC STABILITY OF MICROPROPAGATED SUGARCANE IN DIFFERENT SUBCULTURE FREQUENCIES USING SSR MARKER Alfia Annur Aini Azizi; Ika Roostika; Reflinur Reflinur; Darda Efendi
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.49-57

Abstract

In vitro technique is an effective method to produce high quality and uniform sugarcane seedlings. This study was aimed to determine genetic stability based on SSR marker analysis of six varieties of sugarcane subcultured in regeneration media. It was conducted at the ICABIOGRAD Molecular Biology Laboratory, Bogor, from May 2015 to October 2016. Six sugarcane varieties (PS 862, PS 865, PS 881, PSJK 922, TK 386, and GMP 3) derived from apical shoot explants were subcultured on MS regeneration media enriched with 0.3 mg/l BAP; 0.5 mg/l IBA; and 100 mg/l PVP, for 3, 6 and 9 times. Sugarcane DNA was extracted using the CTAB method; then, the genetic stability was analyzed using 20 pairs of SSR primers. Data were analyzed in groups using the UPGMA method in the SAHN subprogram available on NTSYS software. The results showed that five sugarcane varieties (PS 865, PS 881, PSJK 922, TK 386, and GMP 3) subcultured up to nine times on the regeneration media remained genetically stable with similarity coefficient to their mother plants value more than 0.94.  However, PS 862 variety had genetically unstable after the sixth and the ninth subcultures, the similarity coefficient value to its mother plant was only 0.64, indicated that it experienced somaclonal variations. The study concluded that the in vitro shoots of the other varieties were more genetically stable during subcultures compared to PS 862 sugarcane variety based on SSR marker analysis. Further study is needed to find out the cause of genetic changes in PS 862.Keywords: Saccharum officinarum, apical shoots, in vitro propagation.
Co-Authors , Dorly . Angela Ade Wachjar Ade Wachjar Alfia Annur Aini Azizi ANDRIA AGUSTA Andria Agusta Angela, . Anneke Pesik Asti Kusriyanti Azizi, Alfia Annur Aini Azizi, Alfia Annur Aini Bambang S PURWOKO Bambang S. Purwoko Bambang S. Purwoko Bhaskara, Sandhi Yoga C Hanny Wijaya Cece Suhara Dacholfany, Imanullah Dede Robiatul Adawiyah Deden Derajat Matra Deden Sukmadjaja Dewi Sukma Dianto, Fajar Didy Sopandie DINARTY, DINY Diny Dinarti Djoko Santoso Djoko Santoso Don R LaBonte Dwi Utami Nur Usmani E. Gunawan Edi Santosa Endang Gunawan Entit Hermawan Eny Widajati Erlin Vira Novianti Erwin Al-Hafiizh Evan Maulana Fajar Dianto Fajarudin, A Farida, Naimatul Fitri Fatma Wardani Fitri Fatma Wardani Furqoni, Hafith Gunawan, E. Gustaaf A Wattimena Halimah Widyaningrum Hanifah Muthmainnah Haryanti, Dyra heliyana hermawati Ika Mariska Ika Roostika Ika Roostika IKA ROOSTIKA Ika Roostika Ika Roostika Ika Roostika Tambunan Imanullah Dacholfany Imron Riyadi Inanpi Hidayati Sumiasih, Inanpi Hidayati Indah Wulandari Iswari S Dewi Joko Ridho Witono Kasutjianingati . Katerin Ninariyani Ketty Suketi Kusriyanti, Asti Laela Sari laela Sari, laela Lisnandar, Dea Silvia Lolliani Martin, Andri MASKROMO, ISMAIL Maulana, Evan Maulana, Mohamad Akhbar Maya Melati Mayasari Yamin, Mayasari Megayani Sri Rahayu Mohamad Akhbar Maulana Mutiara Utami Ni Made Wasundhari Dharma Suarka Ninariyani, Katerin Nindita, Anggi NOVARIANTO, HENGKY Nurul Khumaida Nurul Khumaida Odit Ferry Kurniadinata Ogie Satriadi Purwito, Agus Purwito Putra, Mirza R Rahayu, Resa Sri Rahmat Budiarto Rahmi Fajri RARA PUSPITA DEWI LIMA WATI RARA PUSPITA DEWI LIMA WATI, RARA PUSPITA Reflinur Reflinur Resa Sri Rahayu Riry Prihatini Roedhy Poerwanto Rofiq, Muhamad Abdul Rudiyanto Rudiyanto Rudiyanto Rudiyanto, Rudiyanto S Noorrohmah Satriadi, Ogie Sedyo Harsono SEDYO HARTONO Slamet Susanto Sobir Sobir SOEKISMAN TJITROSEMITO Solly Aryza Sony Hartono Wijaya SRI HENDRASTUTI HIDAYAT Sri Yuliani Sri Yuliani Sudarsono Sulassih, . Surya Diantina Surya Kurnia Putra, Dicky Susetio, Muhammad Tambunan, Ika Roostika Tambunan, Ika Roostika Tanari, Yulinda Taruna Shafa Arzam, Taruna Shafa TENDA, ELSJE T. Tiara, Dede TRI ASMIRA DAMAYANTI Tri Istianingsih Tri Muji Ermayanti Tri Muji Ermayanti Tri Muji Ermayanti Trikoesoemaningtyas Triokoesoemaningtyas Triokoesoemaningtyas Vandra Kurniawan Wahyu Fikrinda Wattimena, and Gustaaf Adolf Wida W. Khumaero Willy Bayuardi Suwarno Winarso D. Widodo Witono, dan Joko Ridho Yande Artha Gautama Yosi Zendra Joni Yosi Zendra Joni Yundari, Yundari