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Respon Pertumbuhan Dan Hasil Tanaman Selada (Lactuca sativa L.) Terhadap Pemberian Dosis Pupuk Kandang Sapi Dan Pupuk NPK Dari, Hesti Wulan; Prajaka, Nanang Wahyu; Ali, Fahri; Putri, Sekar Utami
Journal of Horticulture Production Technology Vol 2 No 2 (2024)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jhpt.v2i2.3733

Abstract

In 2020, lettuce production in Indonesia was only 101,129 tons, falling short of the market need of 300,204 tons. Fertilizer effects lettuce plant growth and production. The aim of this study was to establish the optimal dosage of cow manure and NPK fertilizer for lettuce plant development and productivity. The research was conducted on agricultural land in Rawa Selapan Village from April to June 2024. This study used a Randomized Block Design (RAK) with two components and three replications. The first factor, the dose of cow manure, had three levels: without cow manure (P0), 100 g/plant (P1), and 200 g/plant (P2). The second factor, the dose of NPK fertilizer, also had three levels: without NPK fertilizer (N0), 2 g/plant (N1), and 4 g/plant (N2). The collected data were examined using analysis of variance, and if there was a significant difference, a 5% BNT test was performed. According to the study's findings, fertilizing with cow manure at a dose of 200 g/plant resulted in better growth and results in the parameters of plant height, number of leaves, wet weight of plots, wet weight of samples, and dry weight of lettuce roots compared to treatments without manure and cow manure at a dose of 100 g per plant. The application of a dose of NPK fertilizer 4 g/plant resulted in good growth and results in the parameters of plant height, leaf width, wet weight per plot, and wet weight of lettuce roots when compared to treatments without NPK fertilizer and NPK fertilizer 2g/plant. No interaction was found between the combination of cow manure and NPK fertilizer dosages.
Establishment of A Transient Expression Using PEG-Mediated Protoplast Transformation System in Black Rice Cempo Ireng Rahayu Opi Anggoro; Decenly; Yosua Hambit; Nanang Wahyu Prajaka
Journal of Biotropical Research and Nature Technology Vol. 2 No. 1 (2023): Borneo
Publisher : Prodi Biologi FMIPA Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52850/borneo.v2i1.11019

Abstract

Indonesia black rice is a potential crop which consider to be develop as functional food because of high nutritional values. However, some agronomical traits, such as high culm and long harvesting time need to be improved for high productivity. Genetic engineering based on protoplast system is one of tools that can be used for improving black rice agronomical traits. The purpose of this study was to establish an efficient method for obtaining protoplasts, and to get information on whether the PEG-mediated transformation method can be carried out on black rice ‘Cempo Ireng’ using GFP transient expression as a marker. To get protoplast culture, we used callus and seedling as main explants. The results showed that 15th days seedling was the best explant source to get protoplast compare to callus. The combination of 1,5% macerozyme and 3% celulase was optimum to obtain the viable protoplasts. Transient expression of GFP can be done using PEG-mediated protoplast transformation in 30% concentration of PEG.
PENGAPLIKASIAN TEKNOLOGI HIDROPONIK SISTEM DUTCH BUCKET UNTUK MENINGKATKAN KOMPETENSI DAN KETAHANAN PANGAN SANTRI DI PONDOK PESANTREN BAITUL QUR'AN DAARUL AMIIN Ali , Fahri; Tiara, Dede; Rahhutami, Ratih; Prajaka, Nanang Wahyu; Wahyuni, Reza
Jurnal Pengabdian Nasional Vol 6 No 1 (2025)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jpn.v5i2.4154

Abstract

Pondok Pesantren Baitul Qur’an Daarul Amin merupakan pesantren yang ada di Kecamatan Natar, Kabupaten Lampung Selatan, Provinsi Lampung yang memiliki jumlah santri 45 orang dansantriwati 39 orang serta telah meluluskan kurang lebih 80 orang alumni. Kurikulum yang diajarkandalam pondok pesantren tidak hanya dalam bidang ilmu agama saja tepai juga diajarkan softskill yangmenunjang kompetensi santri agar kelak dapat bermanfaat baik untuk diri sendiri maupun untukmasyarakat. Pengembangan softskill yang sedang dijalankan pesantren adalah bidang wirausahadalam bidang perkebunan dan pertanian khususnya tanaman hortikultura. Tim Politeknik NegeriLampung melakukan kegiatan/program pengabdian kepada Masyarakat yang melibatkan para santriPondok Pesantren Baitul Qur’an Daarul Amin untuk berperan aktif dalam kegiatan pengabdiantentang budidaya tanaman melon dengan metode hidroponik sistem Dutch Bucket. Kegiatan inidilakukan beberapa tahap yaitu edukasi, peningkatan keterampilan dan tahap evaluasi. Tahap edukasi adalah tahap pemberian informasi dan ilmu pengetahuan terkait metode hidroponik DutchBucket mulai tahap awal hingga tahap akhir dari serangkaian kegiatan budidaya melon secarahidroponik menggunakan sistem Dutch Bucket. Tahap Pengingkatan keterampilan terdiri dari praktiksecara langsung dalam green house yang ada di dalam pondok pesantren mulai dari persemaianbenih melon, pembuatan instalasi hidroponik, penanaman, pemeliharaan, kegiatan panen dan pascapanen. Tahap terakhir adalah evaluasi selama kegiatan pengabdian. Hasil dari kegiatan pengabdianini para santri meningkat dalam segi kompetensi dalam bidang pertanian melon menggunakanmetode hidroponik sistem Dutch Bucket baik dari segi pengetahuan maupun dari segi praktiklangsung di lapang mulai dari persiapan media tanam sampai proses pemanenan melon hidroponik.Kata kunci: dutch bucket, hidroponik, keterampilan, melon, pesantren
KETAHANAN PENYAKIT PADA CABAI HIBRIDA DAN CABAI LOKAL YANG DIAPLIKASIKAN PESTISIDA NABATI DI PEKON SIDOKATON KECAMATAN GISTING Kartina, Raida; Prajaka, Nanang Wahyu; Darma, Wika Anrya; Putri , Sekar Utami
Jurnal Pengabdian Nasional Vol 6 No 1 (2025)
Publisher : Politeknik Negeri Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25181/jpn.v5i2.4226

Abstract

Kelompok Wanita Tani (KWT) Handayani Putri Kecamatan Gisting merupakan kelompok tani yang aktif dalam budidaya sayur khususnya cabai merah. Produksi cabai gisting tergolong dalam empat wilayah di Provinsi Lampung yang produksi cabainya rendah di Tahun 2022. Salah satu faktor yang mempengaruhi penurunan produksi adalah serangan organisme pengganggu tanaman. Hama utama yang menjadi permasalahan saat ini adalah kutu kebul, kutu daun dan kutu putih. Hal ini perlu adanya pendampingan pada proses budidaya cabai dalam pengendalian hama dan penyakit untuk mendukung optimasi produksi. Pengabdian ini melakukan sosialiasi pembuatan pestisida nabati dengan dua formulasi yang berbeda dan diujikan pada demplot cabai yang berbeda. Cabai yang digunakan dua jenis yaitu cabai hibrida (Sios Tavi F1) dan cabai lokal (Akar F1). Berdasarkan hasil pengabdian masyarakat yang telah berlangsung ketahanan penyakit keriting masih tergolong tinggi dan kuning tergolong rendah. Kejadian penyakit keriting cabai hibrida mencapai 52,13% dan cabai lokal mencapai 30% pada minggu ke-10. Kejadian penyakit kuning pada cabai hibrida 8,5% dan cabai lokal 8,7% dari total tanaman demplot. Kata kunci: penyakit keriting, penyakit kuning, Sios Tavi, Akar
Aplikasi PGPR Akar Bambu dan Akar Putri Malu dalam Mengurangi Intensitas Serangan Aphis gosypii pada Tanaman Cabai (Capsicum annum L.) Safitri, Betari; Febria, Dila; Yeni, Yeni; Prajaka, Nanang Wahyu
Savana Cendana Vol 9 No 3 (2024): Savana Cendana (SC) - July 2024
Publisher : Fakultas Pertanian, Sains, dan Kesehatan, Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/sc.v9i3.2387

Abstract

Plant growth promoting rhizobacter (PGPR) contains Rhizobacter which can fix free nitrogen found in nature, the nitrogen is converted into ammonia which is then distributed to plants. The aim of this research was to determine the effect of administering pgpr from two different bioactivator sources on the intensity of aphid attacks on chili plants. The experimental design used was a Completely Randomized Design (CRD) which consisted of two factors, namely the PGPR concentration of bamboo roots and mimosa roots, namely 0%, 25%, 50%, 75% and 100%. Each treatment was repeated 3 times with each experimental unit consisting of 4 curly chili plants of the Thunder 99 variety. Applications were carried out once a week during the vegetative period. Observations began at 1 WAP and once a week with the observation variables being the type of aphids and the intensity of aphid attacks on each plant during the vegetative period. The results obtained by administering PGPR bamboo roots and putrimalu roots were less effective because they were unable to suppress the intensity of aphid attacks on chili plants. Intensity of aphid attacks not different than control. The level of intensity of aphid attacks is directly proportional to the aphid population. PGPR that can relatively reduce the intensity of aphid attacks is bamboo root treatment with a concentration of 75%. A better PGPR bioactivator comes from bamboo roots rather than mimosa roots.
PENGARUH LIMBAH BAMBU SEBAGAI MEDIA TUMBUH PADA KANDUNGAN PROKSIMAT JAMUR TIRAM (Pleurotus ostreatus) DI PT. BUKIT ASAM-TBK PELABUHAN TARAHAN Ali, Fahri; Prajaka, Nanang Wahyu; Sesanti, Rizka Novi; Maulana, Erie; Mabruroh, Fifki Nugraeni; Hamdani, Hamdani
Agros Journal of Agriculture Science Vol 26, No 1 (2024): Januari
Publisher : Fakultas Pertanian, Universitas Janabadra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37159/jpa.v26i1.4008

Abstract

The oyster mushrooms are generally cultivated on baglog medium, which is produced from leftover wood sawdust. The oyster mushroom may thrive in a variety of cellulose, hemicellulose, and lignin-containing substrates medium. The purpose of this study was to determine the effect of leftover bamboo powder waste on the proximate content of oyster mushrooms when it was utilized as a growing medium in mushroom baglogs. This study was carried out at the Lampung State Polytechnic of Mushrooms Kubung from August to October 2022. The test material consisted of oyster mushrooms collected from mushroom baglogs constructed of 100% sawdust, 100% coarse bamboo waste, 100% fine bamboo waste, and 50% coarse bamboo waste + 50% fine bamboo waste. Three harvests of oyster mushrooms were made on various media types for testing purposes in the lab. According to this study's findings, oyster mushrooms grown on bamboo powder media have a better value than oyster mushrooms grown on wood sawdust media in terms of their proximate content of water, protein, fat, and carbohydrates. Key-words: Bamboo Powder, Nutrition, Oyster Mushroom, Proximate, Wood Sawdust INTISARI Jamur tiram umum ditumbuhkan pada media baglog yang berbahan dasar limbah serbuk gergaji kayu. Jamur tiram memiliki kemampuan untuk tumbuh di berbagai jenis media yang mengandung selulosa, hemiselulosa, dan lignin. Tujuan penelitian ini untuk mengetahui pengaruh dari limbah serbuk bambu yang digunakan sebagai media tumbuh dalam baglog jamur pada kandungan proksimat jamur tiram. Penelitian ini dilaksanakan pada bulan Agustus – Oktober 2022 di Kubung Jamur Politeknik Negeri Lampung. Bahan uji yang digunakan adalah jamur tiram yang dipanen dari baglog jamur berbahan dasar utama Limbah bambu kasar 100%; Limbah bambu halus 100%, Limbah serbuk kayu 100%; dan Limbah bambu kasar 50%+Limbah bambu halus 50%. Jamur tiram yang dipanen pada setiap jenis media dilakukan tiga kali pengulangan untuk keperluan pengujian di dalam laboratorium. Kesimpulan dari penelitian ini adalah kandungan proksimat kadar air, protein, lemak dan karbohidrat jamur tiram yang ditumbuhkan pada media serbuk bambu memiliki nilai lebih tinggi dibandingkan dengan jamur tiram yang ditumbuhkan pada media serbuk gergaji kayu. Kata kunci: Gizi, Jamur tiram, Proksimat, Serbuk bambu, Serbuk gergaji kayu
KERAGAMAN PLASMA NUTFAH BAMBU DI KABUPATEN MALANG JAWA TIMUR Prajaka, Nanang Wahyu; Yulianah, Izmi; Ardiarini, Noer Rahmi
Produksi Tanaman Vol. 5 No. 7 (2017)
Publisher : Jurusan Budidaya Pertanian Fakultas Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bambu merupakan tanaman monokotil (berkeping satu) dan termasuk keluarga rerumputan. Penelitian ini bertujuan untuk mengidentifikasi bambu berdasarkan karakter morfologi, serta mengetahui keragaman jenis bambu yang ada di Kabupaten Malang. Penelitian dilakukan mulai April hingga Juli 2015 di 4 kecamatan di Kabupaten Malang yaitu Kecamatan Tajinan, Kecamatan Wonosari, Kecamatan Wajak, dan Kecamatan Kromengan. Alat yang digunakan meliputi alat tulis, penggaris, form pengamatan karakter morfologi bambu, kamera, meteran, pisau, gunting, parang, gergaji, cetok, pedoman color chart RHS, termometer, klinometer, dan altimeter. Bahan yang digunakan meliputi kertas label, plastik sampel, tali rafia dan plasma nutfah bambu di lokasi penelitian. Metode penelitian yang digunakan adalah metode survei yang meliputi observasi, wawancara dan pengamatan karakter morfologi tanaman bambu. Hasil dari penelitian bambu di Kabupaten Malang diperoleh 13 jenis (spesies) yang termasuk ke dalam 5 marga yaitu Bambusa, Schizostachyum, Gigantochloa, Dendrocalamus dan Phyllostachys. Pada identifikasi tingkat spesies, masing-masing bambu yang ditemukan dengan masih menggunakan nama lokal dapat ditentukan masing-masing nama ilmiahnya. Nama-nama bambu yang ditemukan sebagai berikut Bambu Ampel (Bambusa vulgaris), Bambu Apus (Gigantochloa apus), Bambu Jabal (Schizostachyum aequiramosum), Bambu Jakarta (Phyllostachys aurea), Bambu Jawa (Gigantochloa atter), Bambu Kuning (Bambusa vulgaris var. Striata.), Bambu Ori (Bambusa blumeana), Bambu Petung (Dendrocalamus asper), Bambu Rampal Kuning (Schizostachyum brachycladum), Bambu Rampal (Schizostachyum zollingeri), Bambu Tutul (Bambusa maculata), Bambu Wuluh (Schizostachyum silicatum), dan Bambu Wulung (Gigantochloa atroviolacea).
The Organically Cultivation Techniques of Curly Chili in Handayani Farming Group, Sidokaton Village, Gisting District Kartina, Raida; Prajaka, Nanang Wahyu; Darma, Wika Anrya; Putri, Sekar Utami; Jumawati, Riana
JURNAL PENGABDIAN TEKNOLOGI TEPAT GUNA Vol 5 No 1 (2024): Teknologi Tepat Guna (JPTTG)
Publisher : Universitas Sahid Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47942/jpttg.v5i1.1732

Abstract

Abstract: This community service aims to guide chili farmer groups, especially the Handayani farmer group, to start cultivating chili plants organically on their farming land.There were three stages of the implementation method used, namely increasing knowledge, increasing skills and evaluation. Increasing knowledge was given through lectures to provide knowledge about the cultivation of organic chili plants, botanical pesticides and production of compost made from Azolla pinnata; followed by skills improvement by making botanical pesticides, propagating Azolla pinnata and making compost, demonstration plots of chili plants and then evaluation. Guidance for demonstrations on how to cultivate organic chilies started with planting seeds on the ridge, applying azolla compost to the planting holes, then planting chilies and applying botanical pesticides. Farmers planted chilies in beds that had been made as many as 192 plants which were divided into 6 beds. Afterwards, there were carried out the maintenance activities, controlling pests and diseases with the application of botanical pesticides, and harvesting along with post-harvest handling. This activity would be monitored. Monitoring was carried out once a month, especially at the end of each group of practiced cultivation activities. As an indicator of success, the results of the activity evaluation showed an increase in knowledge of organic farming concepts of 55% and an increase in skills of 85%.   Keywords: Sidokaton village, Pesticide Organic, Azolla pinnata
In Vitro Regeneration of Dendrobium Through Somatic Embryogenesis from Leaf Explants Hamiranti, Rahmadyah; Prajaka, Nanang Wahyu; Yeni, Yeni; Maulida, Desi; Erfa, Lisa
International Journal of Applied Sciences and Smart Technologies Volume 07, Issue 2, December 2025
Publisher : Universitas Sanata Dharma

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24071/ijasst.v7i2.12705

Abstract

The purpose of this study was to determine the best basic medium type and TDZ concentration for the development of somatic embryos from Dendrobium orchid leaf explants. Three replications were arranged factorial (2x3) in a completely randomized design for this study. First, there were two types of basic media, ½ MS and MS. The second factor was the concentration of cytokinin thidiazuron 1, 2 and 3 mg/L. Each experimental unit consisted of 5 culture bottles, each contained 5 explants. The research results showed that 1) The use of both types of basic media was able to induce callus formation on Dendrobium 'Gradita 31' orchid from leaf explants. 2) The use of 3 mg/L thidiazuron which combined with ½ MS or MS media was able to form primary callus faster than other treatments. 3) The higher percentage for embryo somatic and shoots formation were also found in 3 mg/L thidiazuron.