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All Journal Pharmascience Agroprimatech Jurnal Prima Medika Sains Jurnal Ners PRIMER (Prima Medical Journal) Buletin Kedokteran dan Kesehatan Prima Journal of Pharmaceutical and Sciences. Jurnal An-Najat: Jurnal Ilmu Farmasi dan Kesehatan Vitamin: Jurnal Ilmu Kesehatan Umum Galen: Jurnal Ilmu Farmasi dan Kesehatan
Finna Piska
Universitas Prima Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Antibacterial Activity of the Secondary Metabolite of Fusarium LBSU Isolate from the Cat’s Whiskers Plant (Orthosiphon stamineus) Sati Agriani Zega Zega; Rico Halim; Astri Natalia Ginting; Finna Piska
Jurnal Ners Vol. 9 No. 3 (2025): JULI 2025
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v9i3.46029

Abstract

The cat's whisker plant is known to contain bioactive metabolites that have potential as therapeutic agents, while its rhizosphere is a habitat for microorganisms that produce secondary metabolites. Fusarium LBSU isolate were isolate from the rhizosphere of cat’s whiskers plant. Secondary metabolites are produced through liquid fermentation, followed by extraction using ethyl acetate. Antibacterial activity was tested using the disk diffusion method against the Escherichia coli and Staphylococcus aureus. The highest antibacterial activity obtained against Escherichia coli 23.565 mm, higher than Staphylococcus aureus which is 15.3 mm. These findings support the further development of secondary metabolites from rhizosphere isolate as an alternative source of environmentally friendly and effective antibacterial agents.
Uji Aktivitas Ekstrak Daun Salam (Syzygium Polyanthum) terhadap Bakteri Pseudomonas Aeruginosa Secara In Vitro Tice Lestari Ndruru; Muhammad Yunus; Finna Piska
Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan Vol. 2 No. 1 (2026): Galen: Jurnal Riset Ilmu Farmasi dan Kesehatan
Publisher : PT Pustaka Cendekia Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.71417/galen.v2i1.180

Abstract

Pseudomonas aeruginosa merupakan bakteri patogen oportunistik yang sering menginfeksi luka kronis pada penderita diabetes melitus dan memiliki tingkat resistensi antibiotik yang tinggi. Daun salam (Syzygium polyanthum) diketahui mengandung senyawa bioaktif yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun salam terhadap Pseudomonas aeruginosa secara in vitro serta menentukan konsentrasi ekstrak yang paling efektif. Penelitian ini merupakan penelitian eksperimental laboratorium dengan metode difusi cakram (Kirby-Bauer) menggunakan ekstrak daun salam hasil maserasi etanol 96% pada konsentrasi 2,5%, 5%, 10%, 15%, 20%, dan 25%, dengan amoksisilin sebagai kontrol positif dan DMSO sebagai kontrol negatif. Hasil penelitian menunjukkan bahwa ekstrak daun salam memiliki aktivitas antibakteri yang ditandai dengan terbentuknya zona hambat pada seluruh konsentrasi uji, dengan daya hambat yang meningkat seiring peningkatan konsentrasi dan zona hambat terbesar pada konsentrasi 25%, meskipun masih lebih rendah dibandingkan kontrol positif.
Isolasi Protein dan Bakteri Asam Laktat dari Pangan Tradisional Dali Ni Horbo yang Dimodifikasi sebagai Kandidat Probiotik Natasya, Balqis; Meha , Lasminar; Lubis , Asyrun; Lubis , Razoki; Piska , Finna
Journal of Pharmaceutical and Sciences JPS Volume 9 Nomor 1 (2026)
Publisher : Fakultas Farmasi Universitas Tjut Nyak Dhien

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36490/journal-jps.com.v9i1.1367

Abstract

Background: Dali ni horbo is a traditional North Sumatran food made from buffalo milk that has potential as a source of protein and lactic acid bacteria (LAB). Its high nutritional content and fermentation process make it a promising candidate for functional food development with probiotic properties. Objective: This study aimed to isolate and characterize proteins and LAB from dali ni horbo produced using different treatments, namely pure buffalo milk, the addition of pineapple juice, and the addition of citrus juice. Methods: Protein isolation was carried out using the ammonium sulfate precipitation method, while LAB were isolated on MRS agar supplemented with CaCO₃. Protein characterization was performed using biuret, ninhydrin, and xanthoprotein tests. LAB characterization included Gram staining, catalase test, and bile salt tolerance test. Results: The results showed that the protein isolate obtained using pineapple as a coagulant had the highest yield (2.3321 g) compared to citrus (1.8516 g). All protein isolates tested positive for biuret, ninhydrin, and xanthoprotein, indicating the presence of peptide bonds, amino acids, and aromatic groups. A total of 18 LAB isolates were obtained, all of which were Gram-positive, rod-shaped, catalase-negative, and tolerant to bile salts. Conclusion: Dali ni horbo has strong potential as a source of protein and probiotic candidates, indicating its applicability as a functional food to support health.
Co-Authors Astri Natalia Ginting Edy Fachrial Gadis Minanda Fauziah Purba Ginting, Chrismis Novalinda Harmileni Hutauruk, Rico Halim Idaman Lase Jennie Try Varian Laoli Lubis , Asyrun Lubis , Razoki Meha , Lasminar Meutia , Rena Meutia, Rena Muhammad Yunus Nainggolan , Intan Natasya, Balqis Nerly Juli Pranita Simanjuntak Neswita, Elfia Novriani , Erida Nur Ariyani Agustina nurasni, nurasni Olivia Happy Novita Razoki Lubis Razoki Lubis Razoki, Razoki Reh Malem Br Karo Rico Halim Sati Agriani Zega Zega Septia, Evianti Dwi Simanjuntak, Nerly Juli Pranita Tesa, Catrin Angelica Putri Tice Lestari Ndruru Zega, Sati Angriani