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All Journal Dimas: Jurnal Pemikiran Agama untuk Pemberdayaan EduChemia: Jurnal Kimia dan Pendidikan Jurnal Kimia Riset Acta Pharmaciae Indonesia : Acta Pharm Indo Jurnal Agrotek UMMat Journal of Pharmacopolium Jurnal Kartika Kimia J-Dinamika: Jurnal Pengabdian Kepada Masyarakat Jurnal Farmasi Higea Indonesia Natural Research Pharmaceutical Journal (INRPJ) Jurnal Farmagazine Jurnal Mitra Kesehatan Edukasi Masyarakat Sehat Sejahtera (EMaSS) Karya Kesehatan Siwalima Jurnal Farmasi Galenika Sasambo: Jurnal Abdimas (Journal of Community Service)
Anggraeni, Vina Juliana
Bhakti Kencana University
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Aktivitas antioksidan dengan metode cuprac dan penetapan kadar fenolat total pada ekstrak dan fraksi makroalga Eucheuma cottonii Kurnia, Dewi; Rohmah, Desita; Anggraeni, Vina Juliana
Jurnal Agrotek Ummat Vol 9, No 4 (2022): October
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jau.v9i4.11116

Abstract

Antioxidants are compounds that can counteract free radicals. The macroalgae Euchema cottonii belongs to a type of seaweed from the Rhodophyta which is thought to have antioxidant activity. In this study, the antioxidant activity was tested using the CUPRAC method using spectrophotometry at 450 nm and the determination of total phenolic compounds in the extract and macroalgae fraction Eucheuma cottonii using the Folin Ciocalteu method. The extraction method was carried out using the maceration method with 70% ethanol and fractionation using ECC with n-hexane, ethyl acetate, and methanol-water (20% v/v). The results of TLC monitoring of Eucheuma cottonii showed the presence of flavonoid compounds, phenols, alkaloids, steroids, and triterpenoids. The results of the antioxidant activity test showed that the EC50 for the ethanol extract and the n-hexane, ethyl acetate, and methanol-water fractions were 89.11; 143.40; 100.02 and 90.22 g/ml respectively with EC50 vitamin C as a comparison of 6,86 g/ml. The results of the determination of the total phenolate content of the ethanol extract and the fractions of n- hexane, ethyl acetate, and methanol-water were 2.77; 1.96; 2.76; and 3.09 mg/GAE per 100 mg extract. The ethanol extract had a strong antioxidant activity with an EC50  89.11 g/ml and the highest total phenol content was in the methanol-water fraction of 3.09 mg/GAE per 100 mg extract. The results showed that the ethanolic extract of the macroalga Eucheuma cottonii has the potential as an alternative source of antioxidants.
Co-Authors Ade Tika Herawati Ade Tika Herawati Ade Wahyudi Amelia Kandanisa Amelia Kandisa Anne Yuliantini Ariestika, Mutiara Esya Aris Suhardiman Budiana, Wempi Budiana, Wempi Dadang Djuanda Deden Indra Dinata Deden Indra Dinata, Deden Indra Dewi Kurnia Dewi Kurnia Dewi Kurnia Dewi Kurnia Enny Ratnaningsih Fajar Arip Nugraha Fareza, Muhamad Salman Fatmawati, Fenti Garnadi Jafar, Garnadi Idar Idar Idar, Idar Khofiyah, Ikma Nidaul Lela Lailatul Khumaisah Leni Lukitasari Lilis Silviana Liska Ramdanawati Lukitasari, Leni Megawati, Sri Wulan Mutmainah, Siti Saidah Ni Luh Budi Astuti Panjaitan, Riong Seulina Pasha, ED Yunisa Mega Purwaniati Purwaniati Rohmah, Desita Roni, Asep Roni, Asep Sany Yulianti Sita Mardiyani SITI SAIDAH Sri Wulan Megawati Supriyatni Kartadarma Supriyatni Kartadarma Susilawati, Elis Thoriq Nurdin Titis Setyaning Wahyu Wahyudi, Ade Wempi Budiana Winda Ayuantika Yuliantini, Anne ZEILY NURACHMAN Ziska, Rahma