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Identifikasi Bakteri Gram Negatif Galur Extended Spectrum Beta Lactamase Pada Ruang NICU RSUD Prof. DR. W. Z. Johannes Kupang Norma Tiku Kambuno; Dicky Fanggidae
JURNAL INFO KESEHATAN Vol 15 No 2 (2017): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Extended Spectrum Beta-Lactamase (ESBL) is an enzyme that is capable of hydrolyzing antibiotics from penicillin groups, the generation I, II, III cephalosporins, and monobactam. ESBL is most commonly isolated from Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumonia. The spread of ESBL-producing Enterobacteriaceae can also occur due to mutations. Cases of ESBL detection in hospitals have been widely reported throughout the world including Indonesia. The purpose of this study was to identify Enterobacteriaceae which included ESBL strains isolated from the NICU room of RSUD Prof. Dr. W. Z. Johannes Kupang in 2015. This study was a descriptive study with a cross-sectional approach. The samples used were 18 swab specimens from room facilities collected by accidental sampling method. Swab specimens are grown in Blood Agar Plate and Mac Conkey Agar. Bacterial identification methods are equipped with microscopic tests and biochemical tests. Klebsiella sp was identified and then followed by the antimicrobial sensitivity test (Kirby Bauer) against ceftazidime and ceftriaxone. ESBL confirmation test uses the Double Disc Sinergy Test (DDST) method. The results of the antibiotic sensitivity test showed Klebsiella sp. resistant to the antibiotics ceftazidime and ceftriaxone. DDST test shows ESBL production from Klebsiella sp. It was concluded that ESBL-producing Enterobacteriaceae found were Klebsiella sp which had shown resistance to third-generation cephalosporins (Ceftazidime and Ceftriaxone).
Uji Tuberkulosis Laten Pada Kontak Serumah Pasien BTA Positif Dengan Metode Mantoux Test Norma Tiku Kambuno; Yunita Helmina Senge; Agustina W. Djuma; Elisabeth N. Barung
JURNAL INFO KESEHATAN Vol 17 No 1 (2019): Jurnal Info Kesehatan
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol17.Iss1.239

Abstract

Background. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Latent tuberculosis is one of the conditions that occur when exposed to TB bacteria but does not show clinical symptoms. Vulnerable family members infected with TB due to difficulty avoiding contact with patients. The tuberculin skin test is one of the tests used to diagnose latent TB. The purpose of this study was to determine the description of latent TB in-home contact with smear-positive TB patients. Determine the relationship between latent TB with age, gender, education, occupation, and smoking status. Method. The type of research is correlation analytic research with a cross-sectional design with a total sample of 22 people. Results. Based on the results of the study as many as 15 people (68.2%) with induration > 10 mm, and 7 people (31, 8%) with induration <10 mm. While based on the results of statistical tests to see the relationship between the influence of age, sex, education level, occupation and smoking status with latent TB status obtained approx significant> 0.05. Conclusion. Thus the prevalence of latent TB at home contact with positive smear patients was 68.2% and there was no relationship between age, sex, education level, occupation and smoking status with TB status.
Uji Efektivitas Ekstrak Etanol 96% Kulit Jeruk Manis Asal Soe (Citrus sinensis L.) Sebagai Biolarvasida Kuntum Ekawati Nurdin; Loisa Ratna Yuvita Olla; Stefany Febiand Feoh; Anita Dwi Putri Galla; Khusnul Dwi Istnaini; Elisabeth Patricia Febriani Jonison; Norma Tiku Kambuno
JURNAL INFO KESEHATAN Vol 17 No 2 (2019): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol17.Iss2.278

Abstract

One of the plants that can be used as larvicides is sweet orange (Citrus sinensis). Sweet orange rind containing saponins, tannins, flavonoids, and triterpenoids have a characteristic of aromatic odor and bitter taste which contain 96% essential oils containing limonene, glucoside, hesperidium, and resin that can function as biolarvasides. The Regency of the Middle East South is one of the areas in Indonesia which is known as the center of sweet orange. Soe sweet orange fruit has a distinctive color, aroma, and taste compared to other oranges in Indonesia. The objectives of this study are to discover the effectiveness of the orange rind extract in killing the Aedes aegypti larvae and to identify the minimum concentration of the extract of the sweet, dry rind in killing the larvae. This type of research is experimental post-test only control group design. This research was conducted at the Health Analyst laboratory of Kupang Poltekkes (Health Polytechnic) in January 2019. The third (III) instar larvae were placed in 5 vials, each containing 15 larvae. The total number of samples needed was 375 larvae. It was mixed with 15 ml of Soe sweet orange rind with a concentration of 0.075%, 0.1%, 0.25%, 0.50%, 0.75%. One-Way Anova test results obtained sig p-value=0,000 (p<0.05), meaning that there is an influence of sweet orange rind ethanol extract on the death of larvae or orange rind extract is effective as a natural insecticide. Significant values ​​were obtained for all concentrations p=0.008 (p> 0.05) meaning that there was an average difference of each concentration. The conclusion of the study shows that the ethanol extract of sweet orange rind (Citrus sinensis L.) is effective in killing Aedes aegypti mosquito larvae with a minimum concentration of ethanol extract sweet orange rind (Citrus sinensis L.) which can kill 0.075% of larvae.
Differences of Preparation Examination Methods for the Number of Acid-Resistant Bacteria Sherly Dewu; Rahmahsari Bala; Norma Tiku Kambuno
JURNAL INFO KESEHATAN Vol 18 No 1 (2020): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol18.Iss1.390

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis which transmission is sputum less micro-discharge issued by an infected patient. The examination of tuberculosis in acid-resistant bacteria is recommended by WHO using the Ziehl Neelsen method. The objective of this study is to determine the differences in the way the preparations were examined for the number of acid-resistant bacteria. This research is a cross-sectional design laboratory experimental research. The study was conducted in June-July 2018. The examination was conducted microscopically using the Ziehl Neelsen method. The inspection procedure is performed on direct preparations and indirectly checked. The results of the study conducted showed that there was no significant difference in the number of acid-resistant bacteria (p-value = 0.758). It was concluded that the results of examining the number of positive acid-resistant bacteria in direct and indirect preparations examined did not affect the results of the number of bacteria on the microscopic examination of positive acid-resistant bacteria. It is recommended for further studies on the use of uniformed samples for the making of preparations using one of the samples at any time.
Side Effect, Husband Support and Level of Knowledge on the Incidence of Contraceptive Acceptors Dropout Boimau, Adriana M.S; Boimau, Serlyansie V.; Kambuno, Norma Tiku
JURNAL INFO KESEHATAN Vol 21 No 1 (2023): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol21.Iss1.956

Abstract

Family planning program is an effort made by the Indonesian government to decrease the birth rate and control the population. The succuss of family planning program in decreasing the birth rate is influenced by several factors, including the incidence of dropout (DO) regarding the use of contraceptive methods. The high contraceptive acceptors DO rate indicates a problem regarding the use of contraceptive methods. DO cases in Kupang Regency, East Nusa Tenggara (NTT) Province, increased from 1.1% in 2018 to 9.1% in 2021. Therefore, it is necessary to study the factors that cause the increase in the incidence of Contraceptive Acceptors DO. This study aims to determine the factors related to the incidence of Contraceptive Acceptors DO in East Penfui Village, the work area of Tarus Community Health Center, Kupang Regency. This was an analytical survey study with cross-sectional approach. The population involved was family planning acceptors in the village from March to October 2020, as many as 163 acceptors. The samples of 69 acceptors were selected using simple random sampling technique. Data were collected through interviews and documentation. Data were analyzed using Chi-square test. The results revealed that there were three factors which influenced the incidence of contraceptive acceptors DO, namely maternal level of knowledge (p=0.006, husband support for his wife (p=0.003), and side effects regarding contraceptive methods (p=0.014). It can be concluded that a personal approach was needed. Counseling on the side effects of contraception and door to door education can be solutions to be performed by CHC officers as well as existing health cadres.      
Moringa oleifera as Anticancer: A Review of Recent Studies Kambuno, Norma Tiku; Purwaningsih, Erni Hernawati
JURNAL INFO KESEHATAN Vol 22 No 2 (2024): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol22.Iss2.1515

Abstract

Moringa oleifera Lam (MO) plants have long been reported to have many pharmacotherapy benefits. In vitro and in vivo studies have shown that MO extracts have various biological activities and therapeutic effects, including cardioprotective, cardiometabolic, hypocholesterolemic, neuroprotective, anti-inflammatory, antioxidant, anti-hypertensive, anti-diabetic, anti-bacterial, immunomodulatory and anticancer. Researchers have tested extracts from various parts of the MO tree, both in vitro and in vivo, on several types of cancer (such as liver cancer cells, breast cancer, colorectal, leukemia, lung cancer, and oral cancer) with varying success. This review aims to explore the current state of the latest anticancer activity research of MO plants in the last five years. We tried to explore the anticancer activities of MO extracts from reported in vivo and in vitro studies. We searched systematically from three databases (PubMed, Scopus, and Embase) and summarized the data. The keywords used were “Moringa oleifera” AND “anticancer” AND “in vivo” OR “in vitro”. The inclusion criteria were in vivo or in vitro experimental studies and exclusion criteria analyses i.e., in silico trials, study protocols, reviews, or observational studies. This review includes 16 papers on nonclinical studies of MO anticancer activity. Several active compounds have been purified and have reported their anticancer effectiveness, including glucomoringin-ITC/MIC-1, 7-octanoic acid, oleamide, 1-phenyl-2-pentanol, quercetin, gallic acid, p-coumaric acid, and 4-hydroxy 3 – methoxy cinnamic acid, quinic acid. There was no difference in the mechanism of anticancer action based on plant parts, leaves, roots, and seeds, even though using different extraction methods. The general mechanism of action shown was apoptotic, antiproliferative, and cytotoxic. The dose used differed depending on the type of cancer cells used. Some used conventional extraction methods, and others have used modern techniques to extract the purified active compounds from the fractionation process. Our review made it clear that MO could be an excellent and safe candidate for the development of novel therapies against cancer and was most commonly reported in MCF-7, HepG2, and HCT-116 cancer cells. In addition, the development of MO products as future cancer prevention is also interesting to be explored and developed optimally in clinical settings.                         
Hepatitis B Virus Infection in Hemodialisis patient at Prof. DR. W.Z. Johannes Kupang Hospital, East Nusa Tenggara Irfan, Irfan; Wawomeo, Aris; Kambuno, Norma Tiku
JURNAL KESEHATAN PRIMER Vol 4 No 1 (2019): JKP (Jurnal Kesehatan Primer)
Publisher : Poltekkes Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5281/jkp.v4i1.262

Abstract

Background: Hepatitis B or serum hepatitis is a liver infection caused by the hepatitis B virus (VHB) a member of the Hepadnavirus family that can lead to acute and chronic liver inflammation that can continue to be liver cirrhosis or liver cancer. Purpose: This research aims to determine the description of hepatitis B in hemodialysis patients in the Hospital Prof. Dr. W. Z. Johannes Kupang. Methods: This research is a descriptive study. Sampling was done by purposive sampling technique and a total sample of 63 hemodialysis patients. The inclusion criteria of the sample were those had got hemodialysis therapy> 6 months. Data was taken by interview based on a questionnaire to determine the history of hemodialysis, frequency of hemodialysis, history of blood transfusion and examination of blood serum by researchers who were accompanied by laboratory instructors. Results: The results showed that the infection of hepatitis B in hemodialysis patients in hospital Prof. Dr. W.Z. Johannes Kupang from 63 samples obtained 7 positive/reactive HBsAg (11%). Conclusion: History of hemodialysis, frequency of hemodialysis and history of blood transfusion were factors that have the potential to be a source of hepatitis B infection.
Integrated Intervention for Stunting and Tuberculosis Screening in Mothers and Children: A Case Study at Kapan Health Center, East Nusa Tenggara Kambuno, Norma Tiku; Kleden, Simon S; Seran, Agustina A; Nur, Astuti; Peni, Jane A; Mirong, Ignasensia Dua; Nubatonis, Melkisedek O; Baunsele, Novianti P; Bia, Michael B.; Djuma, Agustina W; Valensia, Yualeny
Buletin Penelitian Kesehatan Vol. 53 No. 2 (2025)
Publisher : Poltekkes Kemenkes Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33860/bpk.v53i2.4172

Abstract

Background: The procedure for storing medical record documents (MRDs) involves sorting the records by their medical record numbers before returning them to the medical records department and placing them in storage. This process facilitates the retrieval of required records and streamlines storage operations, thereby preventing misfiling when the documents are needed for patient care. Delays in locating MRDs on the filing racks can result from storage or placement errors, commonly referred to as misfiling. Efficient storage of medical record documents (MRDs) requires proper sorting by medical record numbers before returning them to the filing system. This process ensures quick retrieval and minimizes errors. However, delays in locating MRDs often occur due to misfiling—typically caused by improper storage or placement. This study aims to identify factors contributing to misfiling, focusing on the storage system, alignment, numbering, and human resources. Methods: This study is quantitative research with a descriptive design. The analysis used is univariant analysis. The population comprises inpatient medical records returned from the care units after patient discharge during the period of January to December 2023. The sampling method employed is non-probability sampling, specifically using accidental sampling. Results: Among 50 inpatient MRDs stored in the filing room at Makassar City Regional General Hospital, 15 (30%) were misfiled Conclusion: The factors contributing to the misfiling of medical record documents in the filing room include incompetent medical record personnel, misalignment in the filing system, the absence of tracers, and the lack of an expedition logbook. Misfiling was associated with several factors, including untrained personnel, inconsistencies in the filing alignment, absence of tracers, and the lack of a delivery logbook (previously referred to as an “expedition logbook”) used to track the movement of documents. Addressing these issues may improve filing accuracy and support patient care efficiency.
Associations Between Community Health Worker Capacity and Stunting Reduction Efforts in South Central Timor, Indonesia Pujiyanti, Riny; Irfan, Irfan; Handayani, Fitri; Kleden, Simon Sani; Kambuno, Norma Tiku; Hilaria, Maria; Eziefule, Oluebube Magnificient
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 9 No. 1: JANUARY 2026 - Media Publikasi Promosi Kesehatan Indonesia
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v8i12.8635

Abstract

Introduction: Malnutrition, particularly childhood stunting, remains a critical issue in Indonesia, including the South-Central Timor (SCT) District, East Nusa Tenggara Province. Stunting affects 149.2 million children under five years globally. In the SCT District (2025), stunting prevalence reached 41%. Climatic conditions, food insecurity, and cultural factors contribute to this problem. Prevention efforts involve national strategies, including behavioral change communication campaigns, with Community Health Workers (CHWs) playing crucial roles due to their community credibility and responsiveness. CHW participation is voluntary without continuous commitment obligations. CHW activity and roles in stunting reduction acceleration are paramount, necessitating the investigation of influencing factors. This study aims to identify the factors associated with the roles of Community Health Workers (CHWs) in accelerating stunting reduction in the SCT District in 2025. Methods: This quantitative cross-sectional study included 124 CHWs from 10 community health centers in the SCT District. The study was conducted from January to May 2025. Separate questionnaires measured each variable (CHW role, knowledge, and skills). Ethical approval was obtained from the Kupang Health Polytechnic Research Ethics Committee. Results: Bivariate analysis revealed that knowledge level, education, training history, work duration (all p<0.001), and CHW skills (p=0.002) were significantly associated with CHW roles. The final model showed training history increased CHW role effectiveness by 45.3 times (OR=45.354; 95% CI=1.634-1258.56), CHW skills by 44.3 times (OR=44.393; 95% CI=2.721-724.36), and work duration by 16.9 times (OR=16.934; 95% CI=1.280-224.02). Conclusion: Training, skill level, and work duration were associated with higher CHW role performance, supporting improved stunting prevention efforts. This study provides a new understanding of the importance of enhancing CHW knowledge and skills through structured/routine training programs. Further research comparing CHW capacity in low versus high stunting areas is important for understanding CHW capacity management program improvements.