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Norma Tiku Kambuno
Poltekkes Kemenkes Kupang
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Identifikasi Bakteri Gram Negatif Galur Extended Spectrum Beta Lactamase Pada Ruang NICU RSUD Prof. DR. W. Z. Johannes Kupang Norma Tiku Kambuno; Dicky Fanggidae
JURNAL INFO KESEHATAN Vol 15 No 2 (2017): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

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Abstract

Extended Spectrum Beta-Lactamase (ESBL) is an enzyme that is capable of hydrolyzing antibiotics from penicillin groups, the generation I, II, III cephalosporins, and monobactam. ESBL is most commonly isolated from Enterobacteriaceae, especially Escherichia coli and Klebsiella pneumonia. The spread of ESBL-producing Enterobacteriaceae can also occur due to mutations. Cases of ESBL detection in hospitals have been widely reported throughout the world including Indonesia. The purpose of this study was to identify Enterobacteriaceae which included ESBL strains isolated from the NICU room of RSUD Prof. Dr. W. Z. Johannes Kupang in 2015. This study was a descriptive study with a cross-sectional approach. The samples used were 18 swab specimens from room facilities collected by accidental sampling method. Swab specimens are grown in Blood Agar Plate and Mac Conkey Agar. Bacterial identification methods are equipped with microscopic tests and biochemical tests. Klebsiella sp was identified and then followed by the antimicrobial sensitivity test (Kirby Bauer) against ceftazidime and ceftriaxone. ESBL confirmation test uses the Double Disc Sinergy Test (DDST) method. The results of the antibiotic sensitivity test showed Klebsiella sp. resistant to the antibiotics ceftazidime and ceftriaxone. DDST test shows ESBL production from Klebsiella sp. It was concluded that ESBL-producing Enterobacteriaceae found were Klebsiella sp which had shown resistance to third-generation cephalosporins (Ceftazidime and Ceftriaxone).
Uji Tuberkulosis Laten Pada Kontak Serumah Pasien BTA Positif Dengan Metode Mantoux Test Norma Tiku Kambuno; Yunita Helmina Senge; Agustina W. Djuma; Elisabeth N. Barung
JURNAL INFO KESEHATAN Vol 17 No 1 (2019): Jurnal Info Kesehatan
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol17.Iss1.239

Abstract

Background. Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Latent tuberculosis is one of the conditions that occur when exposed to TB bacteria but does not show clinical symptoms. Vulnerable family members infected with TB due to difficulty avoiding contact with patients. The tuberculin skin test is one of the tests used to diagnose latent TB. The purpose of this study was to determine the description of latent TB in-home contact with smear-positive TB patients. Determine the relationship between latent TB with age, gender, education, occupation, and smoking status. Method. The type of research is correlation analytic research with a cross-sectional design with a total sample of 22 people. Results. Based on the results of the study as many as 15 people (68.2%) with induration > 10 mm, and 7 people (31, 8%) with induration <10 mm. While based on the results of statistical tests to see the relationship between the influence of age, sex, education level, occupation and smoking status with latent TB status obtained approx significant> 0.05. Conclusion. Thus the prevalence of latent TB at home contact with positive smear patients was 68.2% and there was no relationship between age, sex, education level, occupation and smoking status with TB status.
Uji Efektivitas Ekstrak Etanol 96% Kulit Jeruk Manis Asal Soe (Citrus sinensis L.) Sebagai Biolarvasida Kuntum Ekawati Nurdin; Loisa Ratna Yuvita Olla; Stefany Febiand Feoh; Anita Dwi Putri Galla; Khusnul Dwi Istnaini; Elisabeth Patricia Febriani Jonison; Norma Tiku Kambuno
JURNAL INFO KESEHATAN Vol 17 No 2 (2019): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol17.Iss2.278

Abstract

One of the plants that can be used as larvicides is sweet orange (Citrus sinensis). Sweet orange rind containing saponins, tannins, flavonoids, and triterpenoids have a characteristic of aromatic odor and bitter taste which contain 96% essential oils containing limonene, glucoside, hesperidium, and resin that can function as biolarvasides. The Regency of the Middle East South is one of the areas in Indonesia which is known as the center of sweet orange. Soe sweet orange fruit has a distinctive color, aroma, and taste compared to other oranges in Indonesia. The objectives of this study are to discover the effectiveness of the orange rind extract in killing the Aedes aegypti larvae and to identify the minimum concentration of the extract of the sweet, dry rind in killing the larvae. This type of research is experimental post-test only control group design. This research was conducted at the Health Analyst laboratory of Kupang Poltekkes (Health Polytechnic) in January 2019. The third (III) instar larvae were placed in 5 vials, each containing 15 larvae. The total number of samples needed was 375 larvae. It was mixed with 15 ml of Soe sweet orange rind with a concentration of 0.075%, 0.1%, 0.25%, 0.50%, 0.75%. One-Way Anova test results obtained sig p-value=0,000 (p<0.05), meaning that there is an influence of sweet orange rind ethanol extract on the death of larvae or orange rind extract is effective as a natural insecticide. Significant values ​​were obtained for all concentrations p=0.008 (p> 0.05) meaning that there was an average difference of each concentration. The conclusion of the study shows that the ethanol extract of sweet orange rind (Citrus sinensis L.) is effective in killing Aedes aegypti mosquito larvae with a minimum concentration of ethanol extract sweet orange rind (Citrus sinensis L.) which can kill 0.075% of larvae.
Differences of Preparation Examination Methods for the Number of Acid-Resistant Bacteria Sherly Dewu; Rahmahsari Bala; Norma Tiku Kambuno
JURNAL INFO KESEHATAN Vol 18 No 1 (2020): JURNAL INFO KESEHATAN
Publisher : Research and Community Service Unit, Politeknik Kesehatan Kemenkes Kupang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31965/infokes.Vol18.Iss1.390

Abstract

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis which transmission is sputum less micro-discharge issued by an infected patient. The examination of tuberculosis in acid-resistant bacteria is recommended by WHO using the Ziehl Neelsen method. The objective of this study is to determine the differences in the way the preparations were examined for the number of acid-resistant bacteria. This research is a cross-sectional design laboratory experimental research. The study was conducted in June-July 2018. The examination was conducted microscopically using the Ziehl Neelsen method. The inspection procedure is performed on direct preparations and indirectly checked. The results of the study conducted showed that there was no significant difference in the number of acid-resistant bacteria (p-value = 0.758). It was concluded that the results of examining the number of positive acid-resistant bacteria in direct and indirect preparations examined did not affect the results of the number of bacteria on the microscopic examination of positive acid-resistant bacteria. It is recommended for further studies on the use of uniformed samples for the making of preparations using one of the samples at any time.
Co-Authors Agustina W. Djuma Agustina Welhelmina Djuma Akrom, Akrom Anita Dwi Putri Galla Aris Wawomeo Arnawa, I Gede Putu Boimau, Adriana M.S DAVID HANDOJO MULJONO Dicky Fanggidae Donald Emilio Kalonio Elisabeth N Barung Elisabeth N. Barung Elisabeth Patricia Febriani Jonison Erni Hernawati Purwaningsih, Erni Hernawati Fanggidae, Dicky Fernandez, Stefany S.A Ferry WF Waangsir Herlinalt Gloria Banunu, Ayorince Irfan Irfan Irwan Budiana Israfil, Israfil Karol Octrisdey Khusnul Dwi Istnaini Kusmiyati Kusmiyati LOGA, MARIA CARITAS N.A.I Loisa Ratna Yuvita Olla M Ibraar Ayatullah MARIA CARITAS N.A.I LOGA Marni Tangkelangi Meylani Fernanda Bessie MMSI Irfan ,S. Kom Muh. Nasrum Massi Neiny Prisy Foekh Ni Made Susilawati Novelia G So o Nurdin, Kuntum Ekawati Ora, Anderias Parawatu Patmawati, Try Ayu Pius Selasa Rahmahsari Bala Serlyansie V. Boimau Sherly Dewu Soleman Landi Wuan, Adrianus Ola Yos Banne Yunita Helmina Senge Yustina K. Wawo Aja