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All Journal Media Penelitian dan Pengembangan Kesehatan Jurnal Vektor Penyakit Biology, Medicine, & Natural Product Chemistry Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Kedokteran Anatomica (Anatomica Medical Journal) Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Majalah Kedokteran Andalas Jurnal Ners Science Midwifery JURNAL ILMIAH SIMANTEK Nommensen Journal of Medicine Jurnal Visi Eksakta Jurnal Visi Pengabdian Kepada Masyarakat Jurnal Kedokteran Ibnu Nafis Ibnu Sina: Jurnal Kedokteran dan Kesehatan - Fakultas Kedokteran Universitas Islam Sumatera Utara Jurnal Kedokteran Meditek
Simaremare, Ade P
Fakultas Kedokteran Universitas HKBP Nommensen
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Efek Parakrin Sel Punca Mesenkimal pada Kultur In-Vitro Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta
Nommensen Journal of Medicine Vol 9 No 2 (2024): Nommensen Journal of Medicine: Edisi Februari
Publisher : Universitas HKBP Nommensen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36655/njm.v9i2.1410

Abstract

Background: MSC, IL-10, TNF-a, IFN-g, PGE2. Background: The high proliferation and differentiation capabilities, paracrine effects and immunomodulatory functions of Mesencymal Stem Cells (MSCs) have been utilized in cellular therapy approaches for infectious and degenerative diseases in the last decade. Paracrine effects MSCs contain growth factors and cytokines, including IL-10, TNF-a, IFN-g and PGE2. There are still debates regarding the role and performance of the levels of paracrine effects of MSCs IL-10, TNF-a, IFN-g and PGE2, so it is necessary to have a model of the performance of these paracrine effects. Objective: To determine the levels of IL-10, TNF-a, IFN-g and PGE2 in basal in-vitro culture conditions, which can be used as a model for MSC immunomodulatory performance. Methods: This research is an in-vitro culture study of MSCs in basal conditions. MSCs were cultured for 21 days and levels of IL-10, TNF-a, IFN-g and PGE2 were examined on the 7th, 14th and 21st days in the culture medium, using the ELISA method. Results: IL-20 was not found in the culture medium until the 21st day, meanwhile TNF-a levels continued to increase until the 21st day. IFN-g was only found on day 7 of culture. PGE2 levels were found to be the highest secreted among the cytokines analyzed, but were not found on day 21. Conclusion: Paracrine effects secreted by MSCs in basal culture conditions due as a response to cell damage that occurs during the duration of culture. The paracrine effects of MSCs can influence each other, so that there can be suppression or induction of secretion of paracrine effects. Keywords: MSC, IL-10, TNF-a, IFN-g, PGE2. Abstrak Latar belakang: Kemampuan proliferasi dan diferensiasi yang tinggi, efek parakrin serta fungsi imunomudulator Mesencymal Stem Cells (MSCs) dimanfaatkan dalam pendekatan terapi seluler penyakit infeksi dan degeneratif dalam dekade terakhir. Efek parakrin MSCs mengandung growth factor dan sitokin, diantaranya IL-10, TNF-a, IFN-g dan PGE2. Banyak perbedaan pendapat tentang peran dan gambaran kadar efek parakrin MSCs IL-10, TNF-a, IFN-g dan PGE2 sehingga diperlukan adanya model gambaran efek parakrin tersebut. Tujuan: Mengetahui kadar IL-10, TNF-a, PGE2 dan IFN-g pada kondisi basal kultur in-vitro, yang dapat digunakan sebagai model gambaran imunomodulator MSC. Metode: Penelitian ini merupakan penelitian kultur in-vitro MSCs pada kondisi basal. MSCs dikultur selama 21 hari dan dilakukan pemeriksaan kadar IL-10, TNF-a, PGE2 dan IFN-g pada hari ke-7, ke-14 dan ke-21 pada medium kultur, dengan menggunakan metode ELISA. Hasil: IL-20 tidak ditemukan pada medium kultur hingga hari ke-21, sedangkan kadar TNF-a semakin meningkat hingga hari ke-21. IFN-g hanya ditemukan pada kultur hari ke-7. Kadar PGE2 ditemukan paling tinggi disekresikan diantara sitokin yang dianalisa, tetapi tidak ditemukan pada hari ke-21. Kesimpulan: Efek parakrin disekresikan MSC pada kultur kondis basal sebagai respon kerusakan sel yang terjadi selama durasi kultur. Efek parakrin MSC dapat saling mempengaruhi, sehingga dapat terjadi supresi atau induksi sekresi efek parakrin. Kata Kunci: MSC, IL-10, TNF-a, IFN-g, PGE2.
Encapsulated Mesenchymal Stem Cell as Regenerative Alternative for MDR-TB: A Gene Expression Analysis of ABCG2 Efflux Sibuea, Christine Verawaty; Marpaung, Dina Octafrida; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Masal, Cindy Destasya; Laia, George Azriel Buala Nama; Tambunan, Octora Angelica Violyn; Samosir, Wanda Lucia; Sidabalok, Cici Kres
Jurnal Penelitian Pendidikan IPA Vol 11 No 8 (2025): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v11i8.12040

Abstract

Multidrug-resistant tuberculosis (MDR-TB) poses a significant global health challenge due to poor treatment outcomes. Encapsulated mesenchymal stem cells (MSCs) have emerged as a potential alternative therapy; however, their role in modulating bacterial drug sensitivity remains unclear. This study aimed to evaluate the expression of the ABCG2 efflux pump gene in encapsulated MSCs co-cultured with MDR-TB, and to explore its implications for bacterial sensitivity to anti-tuberculosis drugs. An in vitro experimental design was employed using encapsulated MSCs cultured with MDR-TB. Total RNA was isolated, converted into complementary DNA (cDNA), and analyzed using quantitative real-time PCR (RT-PCR). Gene expression levels were quantified using the Livak (ΔΔCt) method. Results demonstrated a progressive increase in ABCG2 gene expression on days 2, 7, and 14. Although this increase may reduce the direct antibacterial capacity of MSCs, previous studies have shown that their preserved paracrine function remains beneficial for immunomodulation. These findings support the continued investigation of encapsulated MSCs as an adjunctive therapy for MDR-TB, particularly through immunoregulatory mechanisms despite increased ABCG2 expression.
TNF-α and IL-10 as paracrine effect of encapsulated mesenchymal stem cells coating by platelet lysate Sibuea, Christine Verawaty; Sitanggang, Ervina Julien; Simaremare, Ade Pryta; Silaen, Rachel Teodora; Kuara , Glenessa; Samosir, Sarah Christina; Ginting, Kharnis Marsha Madora; Yana, Hiqmah Yusi; Pratama, Gita; Mutiara, Mutiara; Angeline, Wiedya Kristianti
Science Midwifery Vol 11 No 6 (2024): February: Midwifery and Health Sciences
Publisher : Institute of Computer Science (IOCS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35335/midwifery.v11i6.1427

Abstract

Mesenchymal stem cells (MSCs) have been used as a cellular therapy for infectious and degenerative diseases due to their paracrine effect, immunomodulatory capability, high ability differentiation, and high plasticity. The paracrine effect of MSCs releases many growth factors and pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), enabling them to modulate the immune system. Nevertheless, there are many obstacles to maintaining paracrine effects in cellular therapy due to a shortage of cellular retention. MSC encapsulation provides a favourable environment for the enhanced viability of MSCs. Platelet lysate is comprised of many growth factors that support the paracrine effect of mesenchymal stem cells (MSCs). In this study, MSCs were encapsulated within alginate, crosslinked using calcium chloride (CaCl2), and subsequently coated with platelet lysate. Encapsulated MSCs coated by platelet lysate were cultured for 21 days and analyzed for IL-10 and TNF-α levels. The findings of our study performed that TNF-α in encapsulated mesenchymal stem cells (MSCs) coated with platelet lysate increased until day 21. IL-10 was retained within the capsule and detected very in day 14. This study showed that encapsulated MSCs coated with platelet lysate affected paracrine effect TNF-α of MSC and retained IL-10 inside the capsule
Perbandingan Kadar PGE2 pada Mikroenkapsulasi MSC dan HSC-CD34 sebagai Potensial Terapi Seluler MDR-TB Simaremare, Ade Pryta R.
Jurnal Ners Vol. 7 No. 2 (2023): OKTOBER 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jn.v7i2.26024

Abstract

Latar belakang penelitian ini untuk melawan Tuberkulosis (TB) telah digunakan obat-obatan selama puluhan tahun, namun selama perjalanannya juga telah menghasilkan strain yang resistan terhadap satu atau lebih obat-obat ini, yang disebut dengan multidrug-resistant tuberculosis (MDR-TB). Terapi konvensional untuk MDR-TB sangat menantang dan membutuhkan waktu yang panjang sehingga dapat menyebabkan efek merugikan yang signifikan terhadap pasien. Selama satu dekade terakhir, pengobatan untuk penyakit degeneratif, infeksi dan metabolik telah berkembang ke arah terapi seluler, khususnya penggunaan terapi stem cell. Stem cell akan mengeluarkan parakrin yang berperan sebagai immunomodulator dan antiinflamasi salah satunya PGE2. Masih belum diketahui bagaimana kadar parakrin PGE2 yang dihasilkan oleh mikroenkapsulasi yang akan digunakan sebagai studi preliminari untuk terapi seluler MDR-TB. Tujuan penelitian ini untuk mengembangkan terapi seluler MDR TB dengan menggunakan parakrin kapsulasi mesenchymal stem cell dan hematopoietic stem cell asal tali pusat. Metode penelitian ini terdiri atas 2 tahap, dimana pada tahap pertama merupakan isolasi, kultur dan enkapsulasi mesenchymal stem cell dan hematopoietic stem cell. Tahap kedua merupakan uji kadar PGE2 pada stem cell yang dimikroenkapsulasi pada hari ke-7, ke-14, dan ke-21. Hasilnya menunjukkan Kadar PGE2 yang dihasilkan oleh mikroenkapsulasi MSC meningkat secara lebih signifikan dibandingkan dengan yang dihasilkan oleh mikroenkapsulasi HSC-CD34. Dengan menggunakan mikroenkapsulasi membuat PGE2 tidak dapat berdifusi keluar sehingga akan menjadi sarana penghantaran yang baik menuju organ yang bermasalah.
Co-Authors Angeline, Wiedya Kristianti Christine Verawaty Sibuea Ginting, Kharnis Marsha Madora Gita Pratama Herald Jonathan Hutabarat Ignes Henny Salim Joseph Partogi Sibarani Joseph Partogi Sibarani Kuara , Glenessa Laia, George Azriel Buala Nama Maduwu, El Yoenay Ivanema Marpaung, Dina Octafrida Masal, Cindy Destasya Masdiwani noviana simanjuntak Meiling Siahaan Micheel Sarah A mutiara mutiara Novreka Pratiwi Sipayung Olivia Yolanda Pasaribu Panggabean, Yeremias Marturia Rocky Regina Saely Sitanggang Rosminta Girsang Rosminta Girsang Saharnaul J Verawaty Simorangkir Saharnauli J Verawaty Simorangkir Saharnauli J. Verawaty Saharnauli Janna Verawaty Simorangkir Samosir, Sarah Christina Samosir, Wanda Lucia Sidabalok, Cici Kres Silaen, Johan Christian Silaen, Rachel Teodora Simanjuntak, Novita Hasiani SISCA SILVANA Sitanggang, Ervina Julien SUHARTOMI . Syarifah, Siti Tambunan, Octora Angelica Violyn Verawaty Simorangkir VINCENSIUS KURNIAWAN ZAI Yana, Hiqmah Yusi Yumiko, Yumiko