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ANTI-BACTERIAL ACTIVITY OF ETHANOL EXTRACT OF INDIAN BORAGE (Coleus Amboinicus) LEAVES AGAINST BACILLUS CEREUS Mutia, Maya Sari; Annisa, Elvia; Suhartomi, Suhartomi
Healthy Tadulako Journal (Jurnal Kesehatan Tadulako) Vol. 7 No. 1 (2021)
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (331.588 KB) | DOI: 10.22487/htj.v7i1.151

Abstract

Bacillus cereus is aerobic, positive gram, and spore-forming bacilli bacteria. The enterotoxin of this bacteria can cause food poisonous that manifest as diarrhea and vomitus. Some previous study has been performed to explore the antibacterial effect of Indian borage leaf, but none of them were explore the antibacterial effect of Indian borage against Bacillus cereus. Hence this study was design to explore the antibacterial activity of ethanol extract of Indian borage against Bacillus cereus. This was an experimental study with post only control group design. Ethanol extract was extracted by maceration methods and antibacterial activity against Bacillus cereus was evaluated by disc diffusion methods. The obtained ethanol extract was dissolved into some concentraions (1 g/ml, 0.8 g/ml, 0.6 g/ml, 0.4 g/ml, 0.2 g/ml). Data was analysed by One Way ANOVA and followed by Post hoc Test Tukey HSD using SPSS 25. Ethanol extract of Coleus amboinicus showed significant difference at the two highest concentration group against the lowest concentration (P- Value < 0.05). The average of inhibition zone diameter from the lowest (0.2 gr/ml) and highest (1.0 gr/ml) concentration were 14.87 mm and 31.50 mm, respectively. Overall, ethanol extract of Indian borage leaves had potential antibacterial activity against Bacillus cereus. This antibacterial activity increase followed by the increase of the concentration.
PENGARUH KEBIASAAN MEROKOK TERHADAP KEJADIAN HIPERTENSI DI RUMAH SAKIT MELATI PERBAUNGAN BRAWA TAMA UNSANDY; SUHARTOMI .
JURNAL ILMIAH MAKSITEK Vol 7 No 1 (2022): JURNAL ILMIAH MAKSITEK
Publisher : LP2MTBM MAKARIOZ

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Abstract

Based on the 2018 Basic Health Research (RISKESDAS), the prevalence of hypertension based on a doctor diagnosis is8.4%. Meanwhile, based on the results of direct measurements of the population aged over 18 years, the prevalence ofhypertension is 34.1%. Therefore, it become important to identify modifiable risk factors. One of them is smoking habit.The purpose of this study was to determine the relationship between smoking and hypertension among inpatients at theInternal Medicine Outpatient Clinic of Melati Perbaungan Hospital in 2020. This study used a cross-sectional design with astudy population of all male patients at the Outpatient Clinic. Fifty-two people were taken from the population by consecutivemethods. All data were then analyzed by SPSS 25 with univariate analysis and bivariate analysis (with chi square test).From this study, it was found that there was a statistically significant relationship between smoking habits (p=0.002), type ofcigarette (p=0.008), length of smoking (p=0.014), and smoking frequency (p=0.011) against hypertension. The activesmoking behavior (OR: 7.20 95% CI: 1.89-27.50), non-filtered cigarette type (OR: 10.80; 95% CI: 1.69-68.94), smokingduration > 20 years (OR: 13.50; 95% CI: 2.15 – 84.69), and the smoking frequency of 1-9 cigarettes/day (OR: 13.50; 95%CI: 2.15-84.69) increased risk of developing hypertension. Hence, it can be concluded that there was a relationship betweensmoking and the develop of hypertension.
HUBUNGAN ANTARA FREKUENSI MENGGUNAKAN ALCOHOL-BASED HAND RUB TERHADAP GAMBARAN KLINIS DERMATITIS KONTAK PADA TENAGA KESEHATAN DI RUMAH SAKIT MURNI TEGUH JOICE SONYA GANI PANJAITAN; SUHARTOMI .
JURNAL ILMIAH SIMANTEK Vol 5 No 3 (2021): JURNAL ILMIAH SIMANTEK
Publisher : LP2MTBM MAKARIOZ

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Abstract

Health workers in operating rooms and Intensive Care Units show high incidence of contact dermatitis, which was 18% to57%. Contact dermatitis in health workers occurs because there are many irritants in hospitals, one of them was antisepticsuch as hand sanitizer or hand rub. This study aimed to evaluate the relationship between the frequency of alcohol-basedhand rub application and the clinical presentation of contact dermatitis in health workers during the COVID-19 pandemic.This study was an observational study with a cross-sectional design. This study was performed among 111 health workers atMurni Teguh Hospital Medan from November 2020-March 2021 who were selected by the purposive sampling method.Meanwhile, the data analysis used to analyze the relationship between the frequency of Alcohol-Based Hand Rub applicationand the clinical presentation of contact dermatitis was the Fisher Exact Test. The majority of health workers at Murni TeguhHospital Medan used alcohol-based hand rub more than 10 times/ day (97.3%). Meanwhile, the clinical presentation ofcontact dermatitis found in health workers from the most to the least was dry skin as many as 103 people (92.8%), followedby thickened skin as many as 92 people (82.9%), burning sensation in 90 people (81.1 %), redness and itching in 88 people(79.3%), and pain in only 84 people (75.7%). After statistical analysis of the frequency of hand rub application and clinicalpresentations of contact dermatitis, no significant relationship was found (P value > 0.05). The frequency of alcohol-basedhand rub application did not affect the clinical presentation of contact dermatitis in health workers at Murni Teguh Hospital.
HUBUNGAN ANTARA ASPEK KESEHATAN MENTAL DAN PERSEPSI TENTANG PEMBELAJARAN DARING TERHADAP INDEKS PRESTASI KUMULATIF MAHASISWA MAUPUN MAHASISWI DI FAKULTAS KEDOKTERAN HKBP NOMMENSEN JOICE SONYA GANI PANJAITAN; SUHARTOMI .
JURNAL ILMIAH SIMANTEK Vol 6 No 1 (2022): JURNAL ILMIAH SIMANTEK
Publisher : LP2MTBM MAKARIOZ

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Abstract

The pandemic has greatly impacted students, resulting in the lecture system that was originally face-to-face to online,moreover the harder task with anxiety about online study achievement. To evaluate the relationship between mental healthaspects and perceptions of online learning system on the grade point average (GPA) among students at the Faculty ofMedicine, Universitas HKBP Nommensen. This study is a cross-sectional study on 88 students at the Faculty of Medicine,Universitas HKBP Nommensen between October-November 2021, selected by the accidental sampling method. Aspects ofmental health (Depression, Anxiety, and Stress) and perceptions about online learning were evaluated by an onlinequestionnaire. Meanwhile, the statistical analysis used to achieve the research objectives was chi-square as bivariateanalysis and logistic regression as multivariate analysis. There was no significant relationship between anxiety, stress, andperceptions of online learning on the GPA of students at the Faculty of Medicine, Universitas HKBP Nommensen (P value >0.05). Interestingly, only depression significantly affected the GPA scores of students at the Faculty of Medicine, UniversitasHKBP Nommensen (P value < 0.05; OR [95% CI]: 4,207 [1,340-13,212]). Only depression significantly affects the GPA ofmedical students during the COVID-19 pandemic.
Pengaruh Persepsi Pembelajaran Daring terhadap Kesehatan Mental Mahasiswa di Fakultas Kedokteran Universitas HKBP Nommensen Joice Sonya Gani Panjaitan; Suhartomi Suhartomi
Majalah Kedokteran Andalas Vol 45, No 3 (2022): Online July 2022
Publisher : Faculty of Medicine, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/mka.v45.i3.p356-366.2022

Abstract

Tujuan: Untuk mengetahui pengaruh persepsi pembelajaran daring terhadap kesehatan mental pada mahasiswa dan mahasiswi di Fakultas Kedokteran Universitas HKBP Nommensen. Metode: Penelitian merpakan penelitian observasional dengan pendekatan potong lintang (cross sectional) pada 86 orang mahasiswa maupun mahasiswi yang dipilih dengan teknik pengambilan sampel accidental sampling. Hasil: Mayoritas responden dalam penelitian ini cenderung menunjukkan gambaran depresi dan stress yaitu sebanyak 61 orang dan 51 orang. Persepsi pembelajaran daring cenderung menyebabkan terjadinya depresi (Nilai P: 0.032; unadjusted OR [IK95%]: 0.208 [0.044-0.975]). Namun, persepsi pembelajaran daring tidak mempengaruhi aspek kesehatan mental lainnya seperti kecemasan (Nilai P: 0.104) maupun stress (Nilai P: 0.103). Namun, setelah dilakukan analisa regresi logistik antara persepsi pembelajaran daring terhadap kesehatan mental dengan memasukkan karakteristik responden dalam model analisa, hubungan antara persepsi pembelajaran daring dan kesehatan mental menjadi tidak signifikan. Kesimpulan: Secara simultan persepsi pembelajaran daring tidak mempengaruhi kesehatan mental mahasiswa maupun mahasiswi fakultas kedokteran dikarenakan keragaman pada karakteristik mahasiswa maupun mahasiswi sebagai responden.
GAMBARAN HASIL PEMERIKSAAN KOH DARI KEROKAN KULIT KAKI PADA PEMULUNG DENGAN TINEA PEDIS DI KOTA MEDAN JOICE SONYA GANI PANJAITAN; SUHARTOMI SUHARTOMI
JURNAL ILMIAH KOHESI Vol 7 No 1 (2023): JURNAL ILMIAH KOHESI
Publisher : LP2MTBM MAKARIOZ

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Abstract

KOH examination of skin scrapings can reveal a variety of microbiological features. Therefore, this study aims to analyze the results of the KOH examination of scavengers who have tinea pedis in Medan City. This research is a cross sectional study of 60 scavengers in Medan City selected by purposive sampling. The results of this study indicate that the majority of scavengers who experience tinea pedis in Medan City are in the Medan Denai area (70%). In addition, the majority of scavengers who experience tinea pedis are women who tend to be 48 years old with a low level of education ranging from uneducated to elementary school (48.3%). From the results of KOH examination of 10% of the skin scrapings, it was found that the most hyphae (80%) and the least were a combination of hyphae and spores (8.3%). Therefore, it can be concluded that the most common KOH examination feature found from skin scrapings of tinea pedis patients is fungal hyphae.
In Vitro Study of Antibacterial Activity of Snake Fruit Extract against Extended Spectrum Beta Lactamase (ESBL) Escherichia coli Linda Chiuman; Sherlyn Sherlyn; Natasya Sabaria Aritonang; Rudy Rudy; Suhartomi Suhartomi
Jurnal Aisyah : Jurnal Ilmu Kesehatan Vol 8, No 2: June 2023
Publisher : Universitas Aisyah Pringsewu

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2182.841 KB) | DOI: 10.30604/jika.v8i2.1962

Abstract

Snake fruit is a tropical fruit that is currently in great interest by the public. The This fruit is known to have high antioxidants so it can prevent an increase in cholesterol and other plasma lipids. Moreover, it can also prevent diarrhea. This study investigated the effective which one of the effective doses (25%, 50%, and 100%) from snake fruit extract against the ESBL-Escherichia coli (E. coli). This study was an experimental study with a post-test-only control group design in Microbiology Laboratory, Universitas Prima Indonesia. To investigate the antibacterial activity of snake fruit extract, this study was used disc diffusion methods which used five different groups including positive control (meropenem), negative control (DMSO), 100%, 50%, and 25% of snake fruit extract. The antibacterial assay showed that it required 100% snake fruit extract to inhibit the growth of ESBL-E. coli with average inhibition zone diameter of 7.33 ± 0.58 mm. The lower concentration extract did not show any antibacterial activity like the negative control group. The positive control group showed the most potent antibacterial activity with an average inhibition zone diameter of 36.33 ± 0.58 mm. Thus, it can be concluded that the snake fruit has antibacterial activity against ESBL-E. coli, but it was not as good as the meropenem. Abstrak: Buah salak merupakan buah tropis yang saat ini banyak diminati oleh masyarakat. Buah ini dikenal memiliki antioksidan yang tinggi sehingga dapat mencegah peningkatan kolesterol dan berbagai lipid plasma. Selain itu, juga dapat mencegah diare. Penelitian ini menyelidiki dosis mana yang efektif (25%, 50%, dan 100%) dari ekstrak salak terhadap ESBL-Escherichia coli (E. coli). Penelitian ini merupakan penelitian eksperimen dengan desain post test only control group di Laboratorium Mikrobiologi Universitas Prima Indonesia. Untuk mengetahui aktivitas antibakteri ekstrak buah salak, penelitian ini menggunakan metode difusi cakram yang menggunakan lima kelompok berbeda yaitu kontrol positif (meropenem), kontrol negatif (DMSO), ekstrak buah salak 100%, 50%, dan 25%. Uji antibakteri menunjukkan bahwa diperlukan 100% ekstrak salak untuk menghambat pertumbuhan ESBL-E. coli dengan diameter zona hambat rata-rata 7.33 ± 0.58 mm. Ekstrak konsentrasi rendah tidak menunjukkan aktivitas antibakteri seperti pada kelompok kontrol negatif. Aktivitas antibakteri paling kuat ditunjukkan oleh kelompok kontrol positif dengan rata-rata diameter zona hambat 36.33 ± 0.58 mm. Dengan demikian dapat disimpulkan bahwa salak memiliki aktivitas antibakteri terhadap ESBL-E. coli, tetapi tidak sebaik meropenem.
Recalcitrant Incomplete Secukinumab Administration in a Psoriasis Patient Gani Panjaitan, Joice Sonya; Suhartomi, Suhartomi
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19100

Abstract

Background: Psoriasis is an immunologic-mediated disease affected by genetic factors that may affect the skin, joints, and cardiovascular system. Some biological agents have been developed and approved by FDA to treat psoriasis. One of these biological agents is Secukinumab, a fully human IgG1κ anti-interleukin-17A(IL-17A) monoclonal antibody. Case Presentation: A seventeen female teenager came to Dermatovenerology Clinic with scaly patches in the forehead and hairline around ten months ago with a history of repeat Corticoticosteroid, DMARDs, and biologic agent treatment. Dermatology examination showed erythema, induration, and desquamation in head and extremities with PASI score of 1.2. She was treated with initial and maintenance doses of Secukinumab Injection.Conclusion: It can be concluded that the recalcitrant administration of Secukinumab in Psoriasis patients may decrease the treatment response.
Analysis of factors influencing smoker behavior in designated non-smoking zone on campus Leonardi, Felix; Trirasetya, Mutiara Ulfa; Suhartomi, Suhartomi; Sihotang, Widya Yanti; Edison, Rizki Edmi
MEDIA ILMU KESEHATAN Vol 13 No 2 (2024): Media Ilmu Kesehatan
Publisher : Universitas Jenderal Achmad Yani Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30989/mik.v13i2.1365

Abstract

Background: The GATS research findings indicate that tobacco use in Indonesia is the highest worldwide among teenagers, with a percentage of 34.8% consumed by individuals aged 15 and above. Tobacco companies leverage this legality to market cigarettes widely to students, including university students. Smoke-free areas are government efforts to prevent smoking on campuses.Objective: To identify determinant factors affecting non-smoking behavior among smokers in smoke-free campus areas.Methods: This research study is descriptive quantitative with a cross-sectional design. It used a purposive sampling technique with criteria of 74 male smoker students from the Faculty of Medicine and Dentistry at Universitas Prima Indonesia and was analyzed using a chi-square test.Results: The chi-square test analysis results indicated an effect between knowledge and attitude on non-smoking behavior in smoke-free campus areas with a P-value of 0.001.Conclusion: The research findings found that knowledge and attitude are determinant factors of non-smoking behavior among smokers in smoke-free campus areas.
Nephroprotective Effects of Sunkist Peel Ethanol Extract on Diabetic Nephropathy Mutia, Maya Sari; Ginting, Chrismis Novalinda; Yulizal, OK; Suhartomi, Suhartomi; Sihotang, Widya Yanti
Majalah Kedokteran Bandung Vol 56, No 3 (2024)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v56.3496

Abstract

The most common long-term diabetic complication is diabetic nephropathy which, in most cases, falls into the End-Stage Kidney Disease. Numerous studies on the nephroprotective effects of citrus fruit have been conducted recently. This study was performed to investigate the phytochemical properties of Sunkist peel ethanol extract and their possible effects on improving diabetic nephropathy. To get the extract, the Sunkist peels were macerated and the extract was analyzed by GC-MS. An in vivo assay of five different groups of 25 male Wistar rats was then performed using the extracts. The groups included control, standard, 500 mg/kg extract, 750 mg/kg extract, and 1,500 mg/kg BW extract. All rats were sacrificed after 28 days of treatment and VEGF level serum and kidney histology analysis were performed. The VEGF data were first analyzed descriptively, followed by one-way ANOVA or Kruskal-Wallis test based on the data distribution. Meanwhile, the kidney histology was observed and narratively described. Results showed that the Sunkist peel ethanol extract has a potential effect to suppress the VEGF level (p-value < 0.05). The extract at the highest dose revealed the mildest pathology damage in the form of tubular structure degeneration without any glomerular damage. This study indicates that Sunkist peel extract has nephroprotective effects from its various phytochemical compounds that work as antioxidants and anti-inflammation.