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All Journal Darussalam Nutrition Journal Bioma : Jurnal Biologi dan Pembelajaran Biologi Medical and Health Science Journal Community Development Journal Biopendix: Jurnal Biologi, Pendidikan dan Terapan Jurnal Abdimas Madani dan Lestari (JAMALI) Amalee: Indonesian Journal of Community Research & Engagement Community Development Journal: Jurnal Pengabdian Masyarakat BERNAS: Jurnal Pengabdian Kepada Masyarakat Medicra (Journal of Medical Laboratory Science/Technology) Journal of Indonesian Medical Laboratory and Science (JoIMedLabS) Environmental and Toxicology Management (ETM) Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Journal of Artificial Intelligence and Digital Business Jurnal Penelitian dan Kajian Ilmiah Kesehatan Politeknik Medica Farma Husada Mataram BIOSAPPHIRE: Jurnal Biologi dan Diversitas
Prayekti, Endah
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Journal : Biopendix: Jurnal Biologi, Pendidikan dan Terapan

 1 
NEW PRIMER DESIGN of MULTIPLEX POLYMERASE CHAIN REACTION (PCR) for DETECTION of Escherichia Coli and Salmonella Enterica in FOOD SAMPLE Hidayat, Muhammad Taufiq; Prayekti, Endah; Lukiyono, Yauwan Tobing; Romdhoni, Muhammad Afwan; Sari, Devi Fitriana
BIOPENDIX: Jurnal Biologi, Pendidikan dan Terapan Vol 11 No 1 (2024): Biopendix: Jurnal Biologi, Pendidikan & Terapan
Publisher : Program Studi Pendidikan Biologi FKIP Unpatti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biopendixvol11issue1page17-25

Abstract

Background: Multiplex PCR techniques are used to detect multiple pathogens at the same time simultaneously. In order to achieve the specificity and sensitivity of detection, it is important to optimize the multiplex PCR method properly. Developing suitable primers and optimizing PCR temperature to boost particular genes from pathogens are key factors for this optimization. Methods: For the simultaneous detection of Escherichia coli and Salmonella enterica in food samples, this research aims to develop a set of primers for multiplex polymerase chain reaction (PCR). Using Primer-BLAST software, the study utilizes specific primer designs for the phoA gene (Eschericia coli) and invA gene (Salmonella enterica). DNA isolation has confirmed successful extraction from the two bacterial samples. PCR was performed under different conditions, including Singleplex and Multiplex PCR, using two annealing temperatures of 53oC and 50oC. Results: The results showed that this method can effectively amplify target genes and indicate their specificity and reliability at both temperature levels. Given these results, it has successfully conducted multiplex PCR using the built primer pairs. Both annealing temperatures of 50oC and 53oC can be used to perform multiplex PCR to detect E. coli and Salmonella enterica in one PCR reaction. Conclusion: Through this research, we have created a new set of Multiplex PCR Primers and an optimized multiplex PCR technique for the simultaneous detection of E coli and Salmonella enterica in food samples. The rapid and simultaneous screening of E. coli and S. enterica, which contributes to improved food safety measures and pathogen detection in the food industry, is promising with this optimized PCR approach.
Co-Authors Akhmad, Nur Faradila andini, ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Awaliyah, Arisma Putri Baihaqi, Muhammad Azhari Devyana Dyah Wulandari Endah Budi Permana Putri Ersalina Nidianti Fakurazi, Sharida Fauziah, Luqiana Fristiyanti, Regina Ayu Gilang Nugraha Hermawati, Septina Isro’ Hidayat, Muhammad Taufiq Junaedy, M Dwinanda Kardina, Rizki Nurmalya Khusnah, Hidayatul Kifli, Nurolaini M Nasir Maghfuroh, Qonita Iklila Mardhotillah, Rachma Rizqina Matin, Nur Sophia Muhammad Afwan Romdloni Mukarramah, Imraatul Nandasari, Anggy Dwipa Navis, Khiliah Pratama, Nizar Revandini Prayoga, Mirshall Abiedama Prisnidiawati, Ajeng Puspitasari, Mia Putri, Ananda Ghufron Yuana Putri, Nasya Desinta Qotrinnada, Nabilah Raharjeng, Sa'bania Hari Rahmawati, Lili Susan Rhomadhoni , Muslikha Nourma Riza, Mochamad Faishal Riza, Mochammad Faishal Rizki Jalil, Ike Wasilatur Romdhoni, Muhammad Afwan Romdloni , Muhammad Afwan Romdloni, Muhmmad Afwan Sabrina, Shinta Milenia Sadza, Lailatus Salimi, Syafrina Maulidyatus Sari, Devi Fitriana Setyana Yuli, Azizah Ari Sofaria Munir, Ratna Suryandaru, Suryandaru Syafiuddin, Achmad Tauziah, Amalia Tullah, Auria Hidayah Uliyatul Laili, Uliyatul Umaha, Farida Wideistianti, Annisa Pricilya Widya Novita Sari Wulan, Wieke Sri Yuliana Yuliana Yuwono, Asma' Denaya Psari Zain, R.A Wafiqah Aulia Zalfa, Anisatuz Zazabilla, Indira