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All Journal Jurnal Fitopatologi Indonesia Jurnal AGROSAINS dan TEKNOLOGI Dinamisia: Jurnal Pengabdian Kepada Masyarakat Jurnal Penelitian Kelapa Sawit Martabe : Jurnal Pengabdian Kepada Masyarakat Bio-Lectura Jurnal Agronomi Tanaman Tropika (JUATIKA) Agro Bali: Agricultural Journal Akta Agrosia Jurnal Agro Indragiri Bhakti Nagori (Jurnal Pengabdian kepada Masyarakat) Bersama: Jurnal Pengabdian Kepada Masyarakat
Andriani, Desta
Universitas Islam Kuantan Singingi
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Environmental Science Languange, Linguistic, Communication & Media Medicine & Pharmacology Social Sciences Other

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Journal : Akta Agrosia

 1 
Characteristics of fungi contaminant in tissue culture of Bromheadia finlaysoniana (Lind.) Miq Andriani, Desta Desta; Heriansyah, Pebra
Akta Agrosia Vol 24 No 2 (2021)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

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Abstract

Generative propagation of orchid plants has problems because orchid seeds do not have endosperm that need to be multiplied using tissue culture. Contamination is a limiting factor in the multiplication of plants in tissue culture. This study describes the types of contaminants found in Bromheadia finlaysoniana callus. This research was carried out in two stages. The first stage is carried out culture of various explants of Bromheadia finlaysoniana using murashige and Skoog media with enrichment of hormone Benzylaminopurine 1 mg/l; (Naphthalene acetic acid) 0.5 mg/l; 100 mg mynositol; pyridoxine-HCl 0.5 mg/l; thiamine-HCl 0.1 mg/l; nicotinic-acid 0.5 mg/l; glycine 2 mg/l. The second stage is observed and the percentage of contaminant fungi. The observed contaminants character includes the colour, the direction of growth, and the hyphae colony's shape. Determination of contaminants type compared to the morphology of references. The result is the highest contamination in flower stalk explants, 81%; most colonies on leaf explants are 28. Contaminant fungi grow predominantly of fungi with white and grey colour and a rough surface shape. The macroscopic character of contaminant fungi in tissue cultures mostly comes from the class of Deuteromycetes and Zygomycetes.Keywords: Bromheadia finlaysoniana ,  contaminant, fungi, tissue culture
Diversity of Endophytic Fungal Community Associated with Local Rice Varieties Commonly Grown in Kuantan Singingi, Riau Province, Indonesia Andriani, Desta; Seprido; Ezward, Chairil
Akta Agrosia Vol 26 No 1 (2023)
Publisher : Badan Penerbitan Fakultas Pertanian (BPFP), Fakultas Pertanian, Universitas Bengkkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/aa.26.1.1-6

Abstract

In a natural ecosystem, the plant establishes a mutual relationship with beneficial endophytes contributing huge impact on its host plant. Therefore, exploring beneficial endophytic fungi identified in a wide range of host plant species and their interaction is an interesting area of study. The present work aimed to characterize the fungal endophytic communities associated with local rice varieties. Healthy local rice variety commonly grown in Kuantan Singingi (Padi Kuning, Padi Gondok, Padi Sironda Putih, and Padi Sironda Merah) were selected and sampled. The samples were taken from different plant parts (root, stem and leaf). Descriptive statistics were used to present  the results of this study. Results obtained from the present study showed that a total of 8 isolates were found in Padi Kuning, 9 isolates in Padi Rondok, 11 isolates in เว็บสล็อต | R4shub Padi Sironda Putih,  and 11 isolates in Padi Sironda Merah. From different tissue parts of the plant, a total of 14, 12, and 13 isolates was found in stem, leaf, and root, respectively. Comparisons of the stem, leaf and root samples demonstrate a similarity in the endophyte assemblages among the local rice varieties and plant parts.
Co-Authors A. Haitami A. HAITAMI A.Haitami Budi Santoso Chairil Ezward, Chairil Delfi Dwi Ningsi Deno Deno Okalia Deno Okalia Deno Okalia Deno Okalia, Deno Dwi Budi Santoso Elfi Indrawanis, Elfi Gusti Marlina GUSTI MARLINA haitami haitami, haitami Ilham Yaumil Pitra M FIRDAUS OKTAFIYANTO Marlina, Gusti Nopriadi Nopriadi Nopsagiarti, Tri Pebra Heriansyah Risdianas Risdianas Seprido Seprido, Seprido Suryo Wiyono Tri Nopsagiarti Tri Nopsagiarti Tri Nur Utami WAHYUDI Wahyudi Wahyudi Wahyudi Wahyudi Wahyudi Wahyudi Widodo Widodo