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All Journal Pharmaciana: Jurnal Kefarmasian THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Jurnal Ilmu Kesehatan Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Jurnal Kesehatan Islam : Islamic Health Journal Medical Laboratory Analysis and Sciences Journal Borneo Journal of Medical Laboratory Technology Journal of Community Service and Empowerment Berkala Ilmiah Kimia Farmasi Prisma Sains: Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Jurnal Abdimas Pamenang Edubiotik : Jurnal Pendidikan, Biologi dan Terapan JURNAL INSAN CENDEKIA
Andyanita Hanif Hermawati
Department Of Medical Laboratory Technology, STIKes Hutama Abdi Husada, Tulungagung, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Pengaruh Serum yang Disimpan Selama Lima Hari Suhu 2-8°C dengan Serum yang Diperiksa Langsung pada Pemeriksaan Kolesterol Total: The Effect of Serum Stored for Five Days Temperature of 2-8°C with Serum That is Checked Directly on Total Cholesterol Testing Chalies Diah Pratiwi; Hariyanto Hariyanto; Andyanita Hanif Hermawati; Intan Niawati Fajrin
Borneo Journal of Medical Laboratory Technology Vol. 4 No. 2 (2022): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v4i2.3790

Abstract

Examination of blood cholesterol at this time became the trend of laboratory examination that much in demand by most people because of many diseases caused by hypercholesterolemia due to wrong diet or high cholesterol levels. Damaged equipment, reagent exhaustion, a large number of samples, laboratory distance with the sampling site too far, and power outages resulted in delayed laboratory examination that required sample storage. This study aimed to determine the effect of serum storage for five days with a serum examined directly to examine total cholesterol. The research design used was an experiment. The population in this research is all female students; Level 3 Study Program D3 Health Analyst STIKes Hutama Abdi Husada Tulungagung, using the Purposive sampling technique, counted 15 respondents. This research was conducted in May 2018. The data obtained were processed using SPSS 16 software and analyzed by Paired t-Test. This study showed the average value of total cholesterol levels in serum examined directly at 163 mg/dl while stored for five days temperature of 2-8 ° C at 158 mg/dl. After the paired test obtained value of P-value = 0.000, so P-value = 0.000 <α = 0.05, it can be concluded there is an influence between total cholesterol levels are checked directly and stored for five days temperature 2-8°C. Five days of serum storage affects the results of total cholesterol tests that lead to a decrease in total cholesterol, but the reduction is insignificant.
Cadmium levels in the urine of cigarette smokers in Bangoan village, Tulungagung district Qurrotu A'yunin Lathifah; Andyanita Hanif Hermawati; Diva Febriani Rahma Kurniawati
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 8 No 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, IKIP Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.2220

Abstract

Cadmium (Cd) which accumulates in the body for a long time can damage human organs such as the respiratory tract, reproductive organs, liver, and kidneys. However, many smokers ignore this, so laboratory evidence is needed to advise smokers. This study analyzed the levels of cadmium (Cd) in the urine of smokers in Bangoan Village, Tulungagung District. The research method used is descriptive analytics. The research sample consisted of 15 smokers obtained through random sampling from 15 cafes in Bangoan Village, Tulungagung Regency. The research instrument was a urine examination result sheet. Urine samples were examined with an Atomic Absorption Spectrophotometer (AAS). The data analysis technique was carried out using quantitative descriptive. The results showed that 8 (53%) smokers had normal cadmium levels, and 7 (47%) other smokers had abnormal cadmium levels with the lowest level being 0.503 µg/L and the highest level being 83.892 µg/L. The community is expected to reduce cigarette consumption and avoid exposure to cigarette smoke in the surrounding environment in order to reduce organ damage.
Analysis of cholesterol and triglyceride levels in obese patients Chalies Diah Pratiwi; Eka Puspitasari; Andyanita Hanif Hermawati
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol 8 No 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, IKIP Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.2518

Abstract

Obesity is excessive fat accumulation in the body. Continuous obesity and excessive food intake can cause metabolic system disorders in the form of hypercholesterolemia. Analysis of cholesterol and triglyceride levels in obese patients needs to be carried out to assist them in making healthier lifestyle changes. This study aims to analyze cholesterol and triglyceride levels in obese patients. The research method used is experimental with a laboratory approach. The samples to be analyzed were 20 obese people obtained by purposive sampling technique in the Tulungagung area. The research instruments used were height and weight check sheets and cholesterol and triglyceride test results sheets. Research data analysis technique by examining cholesterol and triglyceride levels using a MicroLab C-300 spectrophotometer and will be analyzed descriptively quantitatively. The results showed that 15 patients had normal cholesterol levels (75%) with criteria <200 mg/dl and 5 patients had high cholesterol levels (25%) with criteria >200 mg/dl, while there were 17 patients with normal triglyceride levels (85 %) with criteria <150 mg/dl and 3 patients had high triglyceride levels (15%) with criteria >150 mg/dl. The conclusion of this study is that people who are obese do not necessarily have high cholesterol and triglyceride levels. However, when a person weighs > 80 kg, it is recommended to start paying attention to a healthier lifestyle.
Uji Daya Hambat Madu Randu Terhadap Escherichia coli Secara in vitro ANDYANITA HANIF HERMAWATI; Aesthetiza Islamy
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.18206

Abstract

A test of the inhibitory power of randu honey against Eschericia coli ATCC 8739 has been carried out. Honey contains compounds that function as antibacterials. The honey used is monoflora honey, namely randu honey. The concentration of randu honey in the inhibitory power test against Escherichia coli ATCC 8739 is 100% and 50%. The method used is the diffusion method in order to use nutrient media. The inhibitory power of randu honey with a concentration of 100% against Escherichia coli ATCC 8739 is 39.30 mm and the randu honey solution with a concentration of 50% against Escherichia coli ATCC 8739 is 35.80 mm so that randu honey has the potential to be antibacterial.
Differences in Minimum Inhibitory Concentrations of Probiotics and Prebiotics on Staphylococcus aureus Wiwid Yuliastuti; Anyanita Hanif Hermawati; Aesthetica Islamy
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 12, No 1: January 2024
Publisher : IKIP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v12i1.10433

Abstract

Currently, the use of antibiotics has increased sharply and use in the long term will have a bad impact on health. So the emergence of efforts in the form of making natural antibiotics by utilizing materials that are easily available in the environment. One of them is milk, where milk is a food product produced by farm animals, for example cows. This milk can be used as a probiotic. This probiotic is made by adding a bacterium and the bacterium that is often used is Lactobacillus casei. In addition to milk, the material that can be used is telang flower, which is one of the typical plants of Ternate, Maluku with purple, white, pink, and blue petals. Telang flower is often used as a traditional medicine because it has many benefits and content in it. Therefore, in this study Telang flower was used as a prebiotic tested on Staphylococcus aureus bacteria. The study was carried out in vitro by the method of laboratory experiments. The design used is Post Test control group only. The test is repeated at each concentration 3 times to obtain accurate results. The inhibition zone is measured using a caliper. The result of the inhibition zone formed from 3 repetitions is taken as the average value. The test results of the minimum inhibitory concentration of probiotics against Staphylococcus aureus bacteria showed results if at a concentration of 1.25% can form an inhibitory zone of 9.47 mm. While the minimum inhibitory concentration test on prebiotics against Staphylococcus aureus bacteria showed results at a concentration of 2.5% can form an inhibitory zone of 9.02 mm. This suggests that probitiks and prebiotics can be used as antibacterials, since at the lowest concentrations used they can form an inhibitory zone. So the higher the concentration of probiotics and prebiotics used, the inhibition zone formed will be greater.
THE POTENTIAL OF BUTTERFLY PEA FLOWER (Clitoria ternatea) AS AN ANTIBACTERIAL AGAINST Escherichia coli BY IN VITRO Andyanita Hanif Hermawati; Aesthetica , Aesthetica Islamy
Jurnal Kesehatan Islam : Islamic Health Journal Vol. 12 No. 2 (2023): Jurnal Kesehatan Islam : Islamic Health Journal
Publisher : Publikasi oleh Fakultas Kedokteran Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jki.v12i2.20819

Abstract

Abstract. The prevalence of Escherichia coli resistance in Indonesia to antibiotics is 43%. This is a serious problem because it can reduce the efficiency and effectiveness of treatment. Therefore, new products with high antibiotic potential are needed, one of which is telang flower. Telang flowers have active substances with the ability to inhibit bacterial activity. This study aims to test the antibacterial potential of telang flower (Clitoria ternatea) by maceration using 96% ethanol solvent. The research design was an experiment with a control group. The antibacterial testing method used was the agar diffusion method with three repetitions. The telang flower (Clitoria ternatea) extracts used were 50% and 100%. Data were obtained in the form of inhibition zone diameter data. The data were analyzed univariately and bivariate, namely comparing the diameter of the inhibition zone produced from each concentration with the diameter of the inhibition zone of positive and negative controls and bivariate using ANOVA. The results of this study showed that the average diameter of the inhibition zone produced was 15.28 mm (50% concentration) and 19.68 mm (100% concentration). The results of the bivariate analysis showed a p-value = 0.000. This indicates that there is a difference in the diameter of the inhibition zone in all treatment groups. The recommendation from the results of this study is that telang flower extract (Clitoria ternatea) can be continued in vivo research to identify its potential as an alternative antibacterial drug. Keywords: antibacterial; antibiotic; butterfly pea flower; herbal
The Analysis of SGOT, SGPT, and ALP in Chili Farmers Exposed to Pesticides in Kendalbulur Village, Tulungagung Pratiwi, Chalies Diah; Hermawati, Andyanita Hanif; Cahyariza, Nosa Ika
Prisma Sains : Jurnal Pengkajian Ilmu dan Pembelajaran Matematika dan IPA IKIP Mataram Vol 13, No 1: January 2025
Publisher : IKIP Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/j-ps.v13i1.10014

Abstract

Chili farmers in Kendalbulur Village are the main subjects that have a great influence due to exposure to pesticide residues (toxicity). Pesticide toxicity affects enzymes produced by the liver that cause changes in its working system. This study aims to look at the characteristics and levels of the enzymes SGOT, SGPT, and ALP in chili farmers exposed to pesticides that perform spraying activities. The SGOT-SGPT liver function test is a health screening that combines the examination of SGOT and SGPT enzyme levels in the blood. The purpose of this liver function test is to detect hepatitis or cirrhosis of the liver in chili farmers. The alkaline phosphatase (ALP) enzyme test is conducted to help diagnose and evaluate cirrhosis and inflammatory diseases of the liver. In addition to evaluating liver function, the alkaline phosphatase (ALP) enzyme test can also help detect bone disorders or protein deficiencies in chili farmers. Respondents were 20 chili farmers and pesticide users with an age range of 34–71 years. Test method using the non-analytical descriptive method and sampling technique using total sampling. The examination carried out is the rate of SGOT, SGPT, and ALP using spectrophotometry. The results of the SGOT examination showed that all respondents had SGOT levels in the normal range of <40 U/L, where the lowest result was 16 U/L and the highest was 35 U/L. While SGPT showed that all respondents were in the normal range of 40 U/L, with the lowest result of 2 U/L and the highest of 23 U/L, the ALP levels of many respondents were above normal values. The lowest value is 131 U/L, and the highest is 222 U/L. The results showed that the respondents showed disruption of the liver because ALP levels give high results. Where should ALP levels decrease with age.
UJI ANTIBIOTIK CIPROFLOXACIN TERHADAP PERTUMBUHAN ESCHERICHIA COLI SECARA IN VITRO Andyanita Hanif Hermawati; Surtini Surtini; A’yuni Latifa Arohman; Hariyanto Hariyanto
Jurnal Insan Cendekia Vol 10 No 3 (2023): Jurnal Insan Cendekia
Publisher : STIKES Insan Cendekia Medika Jombang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35874/jic.v10i3.1187

Abstract

Pendahuluan: Escherichia coli (E. coli) merupakan bakteri yang dapat hidup dan bertahan hidup di saluran pencernaan. Infeksi yang disebabkan oleh E. coli dapat dihambat dengan pemberian antibiotik. Ciprofloxacin merupakan antibiotik yang digunakan untuk mengobati infeksi yang disebabkan oleh berbagai bakteri, salah satunya adalah E. coli. Namun, saat ini, banyak insiden resistensi antibiotik meningkatkan lamanya seseorang menderita suatu penyakit dan lamanya tinggal di rumah sakit. Tujuan: Untuk melihat sejauh mana pengaruh efektivitas antibiotik yang sering diresepkan bagi penderita infeksi, pengujian antibiotik dapat dilakukan dengan pengujian sensitivitas. Penelitian ini bertujuan untuk mengetahui pengaruh penggunaan antibiotik ciprofloxacin terhadap pertumbuhan bakteri Escherichia coli secara in vitro. Penelitian ini dilakukan di Laboratorium Mikrobiologi STIKes Hutama Abdi Husada Tulungagung. Penelitian ini merupakan penelitian laboratorium eksperimental. Sampel penelitian ini adalah isolat bakteri E. coli. Metode: Metode yang digunakan adalah uji difusi cakram, menggunakan konsentrasi ciprofloxacin 125 mg/ml, 250 mg/ml, 500 mg/ml, dan 750 mg/ml. Hasil: Hasil penelitian pada konsentrasi ciprofloxacin 500 mg/ml dan 750 mg/ml dengan 6 kali pengulangan menunjukkan terbentuknya zona hambat >21 mm yang artinya sensitif terhadap bakteri E. coli. Kesimpulan: Penelitian ini menyimpulkan bahwa pengaruh penggunaan antibiotik ciprofloxacin terhadap bakteri E. coli secara in vitro bersifat sensitif. Hal ini dikarenakan ciprofloxacin masih dapat bekerja menghambat aktivitas topoisomerase II (DNA girase) dan topoisomerase IV yang dibutuhkan oleh E. coli untuk replikasi DNA. Dari hasil penelitian tersebut, ciprofloxacin masih layak digunakan sebagai antibiotik untuk mengobati infeksi yang disebabkan oleh E. coli.
Kadar Timbal (Pb) pada Petugas SPBU di Tulungagung Lathifah, Qurrotu A'yunin; Hermawati, Anyanita Hanif; Tristanti, Duwi
Borneo Journal of Medical Laboratory Technology Vol. 7 No. 2 (2025): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v7i2.9366

Abstract

Plumbum (Pb) adalah logam berat yang secara alami terdapat di dalam kerak bumi dan pada asap kendaraan berbahan bakar bensin. Unsur ini terdapat pada pembakaran knalpot kendaraan dan dari uap bensin, hal ini dapat dijumpai pada petugas SPBU yang terpapar Plumbum dari pembakaran knalpot kendaraan dan uap bensin dapat meningkatkan kadar Plumbum. Tujuan penelitian ini adalah untuk mengetahui kadar Plumbum (Pb) dalam urine pada petugas Stasiun Pengisian Bahan Bakar Umum (SPBU) di Kabupaten Tulungagung. Metode yang digunakan yaitu Deskriptif Non Analitik. Sampel penelitian ini adalah urine petugas SPBU di Kabupaten Tulungagung yang diambil menggunakan teknik Random sampling. Jumlah sampel Urine yang didapatkan 25 sampel. Pemeriksaan dilakukan di Laboratorium Kimia Universitas Muhamadiyah Malang menggunakan metode Spektrofotrometri Serapan Atom (SSA). Hasil pemeriksaan sampel urine petugas SPBU menunjukkan kadar Pb rata-rata 0,039 µg/L, dimana nilai normal kadar Pb yaitu ≤ 10 µg/L. Kesimpulan dari penelitian ini adalah kadar Pb pada Petugas SPBU seluruhnya normal. Penggunaan APD sangat penting untuk kesehatan petugas SPBU. Petugas SPBU seharusnya menggunakan APD saat bekerja untuk mengurangi paparan dari uap bensin.
PENYULUHAN CUCI TANGAN PAKAI SABUN PADA ANAK USIA DINI DALAM MENDORONG PERILAKU HIDUP BERSIH DAN SEHAT: COUNSELING HAND WASHING WITH SOAP IN EARLY CHILDHOOD IN ENCOURAGING CLEAN AND HEALTHY LIVING BEHAVIOR Asna Lailatul Fadhila; Fina Fitrotul Azlina; Regina Yulianto; Vaneka Shukma Dewanti; Andyanita Hanif Hermawati; Intan Munawaroh
Jurnal Abdimas Pamenang Vol. 3 No. 2 (2025): Jurnal Abdimas Pamenang - JAP
Publisher : STIKES Pamenang Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53599/jap.v3i2.354

Abstract

Penyuluhan kesehatan mengenai Perilaku Hidup Bersih dan Sehat (PHBS) merupakan upaya penting dalam membentuk kebiasaan hidup sehat sejak usia dini. Salah satu aspek utama dari PHBS adalah praktik mencuci tangan yang baik dan benar. Program pengabdian kepada masyarakat ini dilakukan di Desa Kedungwaru Kabupaten Tulungagung pada Bulan November Tahun 2024. Media yang digunakan dengan pemaparan materi tentang cuci tangan, menampilkan video edukasi, serta demonstrasi mengunakan games dan diberikan konseling kepada anak-anak dan diberikan poster. Pemaparan 7 Langkah Mencuci Tangan kegiatan diawali dengan perkenalan anggota, anggota kelompok memperkenalkan dirinya masing-masing kepada murid Paud Anak Bangsa, Kegiatan ini bertujuan untuk meningkatkan pengetahuan dan keterampilan anak usia 3–4 tahun dalam melakukan cuci tangan sesuai dengan langkah-langkah yang tepat. Melalui pendekatan edukatif yang interaktif dan metode pembelajaran yang disesuaikan dengan perkembangan usia dini, anak-anak diajarkan tujuh langkah mencuci tangan menggunakan sabun dan air mengalir. Hasil penyuluhan menunjukkan bahwa anak-anak dapat memahami dan mempraktikkan kebiasaan mencuci tangan dengan benar, sehingga diharapkan dapat menurunkan risiko infeksi penyakit dan meningkatkan derajat kesehatan mereka. Penyuluhan ini menegaskan pentingnya pendidikan kesehatan sejak dini dalam membangun perilaku hidup bersih dan sehat yang berkelanjutan.  Kata Kunci: PHBS, Cuci tangan, Anak Usia Dini, Pendidikan Kesehatan   Abstract Health counseling on Clean and Healthy Living Behavior (PHBS) is an important effort in forming healthy living habits from an early age. One of the main aspects of PHBS is the practice of washing hands properly and correctly. This community service program was carried out in Kedungwaru Village, Tulungagung Regency in November 2024. The media used were the presentation of material on washing hands, showing educational videos, and demonstrations using games and counseling was given to children and posters were given. The presentation of the Seven Steps to Washing Hands activity began with the introduction of members, group members introduced themselves to the students of Paud Anak Bangsa, This activity aims to improve the knowledge and skills of children aged 3-4 years in washing their hands according to the correct steps. Through an interactive educational approach and learning methods that are adapted to early age development, children are taught the seven steps of washing hands using soap and running water. The results of the counseling show that children can understand and practice the habit of washing their hands properly, so that it is expected to reduce the risk of disease infection and improve their health. This counseling emphasizes the importance of early health education in building sustainable clean and healthy living behavior.  Keywords: PHBS, Hand washing, Early Childhood, Health Education
Co-Authors Aesthetica , Aesthetica Islamy Aesthetica Islamy Aesthetiza Islamy Anggi Yupita Putri Anis Murniati Aryati Aryati Asna Lailatul Fadhila Aulia Zahratul Husna A’yuni Latifa Arohman Cahyariza, Nosa Ika Chalies Diah Pratiwi Chalies Diah Pratiwi Chalies Diah Pratiwi Cholila Lailatul Nurmala Deny Yusnia Milasari Diva Febriani Rahma Kurniawati Eka Puspitasari Eka Puspitasari, Eka Fina Fitrotul Azlina HARIYANTO HARIYANTO Hariyanto Hariyanto Hariyanto Hariyanto Haryanto, Isnaeni Yudi Intan Munawaroh Intan Niawati Fajrin Islamy, Aesthetiza Isnaeni Isnaeni Yudi Haryanto Kurniawati, Diva Febriani Rahma Lathifah, Qurrotu A'yunin Lathifah, Qurrotu A’yunin Nosa Ika Cahyariza Prastama, Hizkia Yustin Pratiwi, Chalies Diah Qurrotu A’yunin Lathifah Regina Yulianto Riska Miftakhul Jannah Suhandayati, Elviana Surtini Surtini Tristanti, Duwi Turista, Dora Dayu Rahma Vaneka Shukma Dewanti Wiwid Yuliastuti