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Comparison of the inhibitory effects of butterfly pea flower (Clitoria ternatea L.) extract and fermented milk probiotics on the growth of Salmonella sp. Islamy, Aesthetica; Hermawati, Andyanita; Yuliastuti, Wiwid
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 10 No. 01 (2025): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v10i01.795

Abstract

The increasing resistance of pathogenic bacteria such as Salmonella sp. to antibiotics has prompted the need for alternative antibacterial agents derived from natural sources. This study aimed to compare the inhibitory effects of prebiotics and probiotics against Salmonella sp. in vitro. The research was a laboratory experimental study using a post-test-only control group design. The prebiotic used was derived from butterfly pea flower (Clitoria ternatea L.) extract, while the probiotic was obtained from cow’s milk fermented with Lactobacillus casei. The test organism was Salmonella sp., obtained from a pure culture. The well diffusion method was used to evaluate antibacterial activity by measuring the diameter of the inhibition zone using a vernier caliper. Five concentrations (0.65%, 1.25%, 2.5%, 5%, and 10%) were tested in triplicate. The data were analyzed using descriptive statistics to calculate the mean inhibition zone diameter. At a concentration of 1.25%, the average inhibition zone of the probiotic was 8.08 mm, while the prebiotic produced a zone of 7.4 mm. Both agents showed larger inhibition zones compared to the negative control. The findings indicate that both prebiotics and probiotics exhibit antibacterial activity against Salmonella sp., with probiotics showing slightly stronger effects. These substances have the potential to be developed as natural antibacterial agents.
Uji Daya Hambat Madu Randu Terhadap Escherichia coli Secara in vitro HERMAWATI, ANDYANITA HANIF; Islamy, Aesthetiza
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 6 No 2 (2023): The Journal Of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v6i2.18206

Abstract

A test of the inhibitory power of randu honey against Eschericia coli ATCC 8739 has been carried out. Honey contains compounds that function as antibacterials. The honey used is monoflora honey, namely randu honey. The concentration of randu honey in the inhibitory power test against Escherichia coli ATCC 8739 is 100% and 50%. The method used is the diffusion method in order to use nutrient media. The inhibitory power of randu honey with a concentration of 100% against Escherichia coli ATCC 8739 is 39.30 mm and the randu honey solution with a concentration of 50% against Escherichia coli ATCC 8739 is 35.80 mm so that randu honey has the potential to be antibacterial.
Analysis of cholesterol and triglyceride levels in obese patients Pratiwi, Chalies Diah; Puspitasari, Eka; Hermawati, Andyanita Hanif
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 8 No. 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.1061

Abstract

Obesity is excessive fat accumulation in the body. Continuous obesity and excessive food intake can cause metabolic system disorders in the form of hypercholesterolemia. Analysis of cholesterol and triglyceride levels in obese patients needs to be carried out to assist them in making healthier lifestyle changes. This study aims to analyze cholesterol and triglyceride levels in obese patients. The research method used is experimental with a laboratory approach. The samples to be analyzed were 20 obese people obtained by purposive sampling technique in the Tulungagung area. The research instruments used were height and weight check sheets and cholesterol and triglyceride test results sheets. Research data analysis technique by examining cholesterol and triglyceride levels using a MicroLab C-300 spectrophotometer and will be analyzed descriptively quantitatively. The results showed that 15 patients had normal cholesterol levels (75%) with criteria <200 mg/dl and 5 patients had high cholesterol levels (25%) with criteria >200 mg/dl, while there were 17 patients with normal triglyceride levels (85 %) with criteria <150 mg/dl and 3 patients had high triglyceride levels (15%) with criteria >150 mg/dl. The conclusion of this study is that people who are obese do not necessarily have high cholesterol and triglyceride levels. However, when a person weighs > 80 kg, it is recommended to start paying attention to a healthier lifestyle.
Cadmium levels in the urine of cigarette smokers in Bangoan village, Tulungagung district Lathifah, Qurrotu A’yunin; Hermawati, Andyanita Hanif; Kurniawati, Diva Febriani Rahma
Edubiotik : Jurnal Pendidikan, Biologi dan Terapan Vol. 8 No. 01 (2023): Edubiotik : Jurnal Pendidikan, Biologi dan Terapan
Publisher : Biology Education Department, Universitas Insan Budi Utomo, Malang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33503/ebio.v8i01.1062

Abstract

Cadmium (Cd) which accumulates in the body for a long time can damage human organs such as the respiratory tract, reproductive organs, liver, and kidneys. However, many smokers ignore this, so laboratory evidence is needed to advise smokers. This study analyzed the levels of cadmium (Cd) in the urine of smokers in Bangoan Village, Tulungagung District. The research method used is descriptive analytics. The research sample consisted of 15 smokers obtained through random sampling from 15 cafes in Bangoan Village, Tulungagung Regency. The research instrument was a urine examination result sheet. Urine samples were examined with an Atomic Absorption Spectrophotometer (AAS). The data analysis technique was carried out using quantitative descriptive. The results showed that 8 (53%) smokers had normal cadmium levels, and 7 (47%) other smokers had abnormal cadmium levels with the lowest level being 0.503 µg/L and the highest level being 83.892 µg/L. The community is expected to reduce cigarette consumption and avoid exposure to cigarette smoke in the surrounding environment in order to reduce organ damage.
Gambaran Profil Salmonella Sp. Pada Getuk: Profile Of Salmonella sp. In Getuk Suhandayati, Elviana; Hermawati, Andyanita Hanif; Hariyanto, Hariyanto; Cahyariza, Nosa Ika
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.5996

Abstract

Getuk merupakan makanan ringan yang terbuat dengan bahan utama ketela pohon atau singkong. Bakteri yang menyebabkan diare atau foodborne diease masuk melalui berbagai cara yaitu oral, lingkungan yang tercemar, makanan, dan lain-lain sehingga kondisi seperti ini sangatlah tergantung dengan pedagang, bagaimana pedagang tersebut tetap mempertahankan kehigienisan makanan yang dijualnya agar tidak terkontaminasi. Tujuan dari penelitian ini adalah untuk mengidentifikasi bakteri Salmonella sp. pada makanan tradisional getuk yang dijual di Pasar Ngunut. Penelitian ini merupakan penelitian deskriptif. Yang dilaksanakan pada 24 sampai 29 Mei 2018 di Laboratorium Mikrobiologi DIII Teknologi Laboratorium Medis STIKes Hutama Abdi Husada Tulungagung. Populasi pada penelitian ini adalah semua getuk yang dijual di Pasar Ngunut, teknik sampling pada penelitian ini adalah total sampling, yang diperoleh sebanyak 6 sampel makanan tradisional getuk. Instrumen yang digunakan diantaranya adalah tabung steril, pipet ukur, incubator, plate, media agar plate, aquadest, oven dan peralatan laboratorium yang dibutuhkan. Hasil penelitian diperoleh bahwa dari 6 sampel, 3 sampel positif bakteri Salmonella dan 3 sampel tidak ditemukan bakteri Salmonella sp. Hasil penelitian selanjutnya dianalisis secara diskriptif. Dari hasil penelitian yang telah dilakukan dapat disimpulkan bahwa sampel teridentifikasi bakteri Salmonella sp pada makanan tradisional getuk yang dijual di Pasar Ngunut Tulungagung dengan menggunakan media Selenite Broth sebagai pemupuk, SSA sebagai media selektif, KIA untuk melihat Gas dan H2S, MIU untuk meliat urea dan motilitas bakteri, serta pengecatan Gram untuk melihat Gram Negatip batang
Perbedaan EDTA Konvensional Dan EDTA Vacutainer Pada Pemeriksaan Kadar Hemoglobin: Differences Between Conventional EDTA And Vacutainer EDTA In Hemoglobin Level Examination Hariyanto, Hariyanto; Hermawati, Andyanita Hanif; Prastama, Hizkia Yustin
Borneo Journal of Medical Laboratory Technology Vol. 6 No. 2 (2024): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v6i2.7102

Abstract

Penelitian ini bertujuan untuk mengevaluasi perbedaan hasil kadar hemoglobin yang diukur menggunakan EDTA Konvensional dan EDTA Vacutainer. Desain penelitian yang digunakan adalah pre-eksperimen, dengan total 30 sampel yang diambil dari populasi mahasiswi prodi TLM STIKes Hutama Abdi Husada Tulungagung. Hasil analisis menunjukkan rata-rata kadar hemoglobin menggunakan EDTA Konvensional sebesar 15.6 g/dL, sedangkan menggunakan EDTA Vacutainer sebesar 15.2 g/dL. Meskipun terdapat perbedaan, uji statistik menunjukkan bahwa perbedaan tersebut tidak signifikan secara statistik (p = 0.425). Kesimpulan dari penelitian ini adalah tidak ada perbedaan yang signifikan dalam hasil kadar hemoglobin antara penggunaan EDTA Konvensional dan EDTA Vacutainer. Namun, terdapat beberapa keterbatasan dalam penelitian ini, termasuk keterbatasan waktu pengambilan sampel dan adanya responden yang sedang menstruasi. Antikoagulan seperti EDTA digunakan untuk mencegah pembekuan sampel.
DUKUNGAN KELUARGA DALAM PERAWATAN SELF-CARE TERHADAP KUALITAS HIDUP PENDERITA DIABETES MELLITUS Murniati, Anis; Munawaroh, Intan; Hermawati, Andyanita Hanif
coba Vol 14 No 1 (2025): November 2025
Publisher : Akademi Keperawatan Dharma Husada Kediri

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32831/jik.v14i1.912

Abstract

Diabetes Mellitus (DM) adalah penyakit kronis yang memerlukan pengelolaan self-care yang baik untuk mencegah komplikasi dan meningkatkan kualitas hidup. Dukungan keluarga menjadi faktor penting dalam keberhasilan perawatan mandiri. Metode: Penelitian ini menggunakan desain cross sectional pada 33 responden keluarga penderita DM di Desa Bangoan, Tulungagung, dengan teknik total sampling. Instrumen yang digunakan adalah kuesioner Hensarling Diabetes Family Support Scale (HDFSS) dan Diabetes Quality of Life (DQOL) yang telah dimodifikasi. Analisis dilakukan secara univariat dan bivariat. Diskusi: sebagian besar responden memiliki dukungan keluarga tinggi (72,7%) dan kualitas hidup tinggi (57,6%). Dukungan emosional dan penghargaan tinggi ditemukan pada semua responden. Terdapat hubungan signifikan antara dukungan keluarga dan kualitas hidup, di mana dukungan tinggi berasosiasi dengan kualitas hidup tinggi. Kesimpulan: Dukungan keluarga yang optimal berperan penting dalam meningkatkan kepatuhan self-care dan kualitas hidup penderita DM. Intervensi keluarga dan edukasi berkelanjutan diperlukan untuk memaksimalkan hasil perawatan. Kata Kunci: Diabetes mellitus, dukungan keluarga, kualitas hidup, self-care
The effect of counseling on hemoglobin on the knowledge of Islamic boarding school students Chalies Diah Pratiwi; Andyanita Hanif Hermawati; Nosa Ika Cahyariza
Journal of Community Service and Empowerment Vol. 4 No. 3 (2023): December
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jcse.v4i3.28242

Abstract

Anemia is a condition where the mass of erythrocytes or hemoglobin in circulation cannot fulfill its function to provide oxygen to body tissues. Anemia in adolescent girls is still quite high; the world prevalence of anemia ranges from 40 to 88%. Community service activities aim to screen (check) hemoglobin levels as early detection of anemia using the Point of Care Testing (POCT) method to determine the relationship between age and education to the incidence of anemia and as a preventive effort to minimize anemia. This activity was carried out through counseling about hemoglobin using the lecture method at the Islamic Boarding School Roudlotul Musthofa in May with 23 female students as respondents, which were then analyzed using the Wilcoxon statistical test. The results of the questionnaire before counseling (pre-test) obtained an average of 80, then counseling was carried out, and the average results of the questionnaire (post-test) were 94. The results of the Wilcoxon Test analysis showed p (Asymp.Sig. (2-tailed)) = 0.000 < α (0.05), which means that there is a significant influence between the knowledge of junior high school students of Raudlatul Mustofa Islamic Boarding School Tulungagung Regency and hemoglobin counseling conducted.