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All Journal Jurnal Pengolahan Hasil Perikanan Indonesia METAMORFOSA Journal of Biological Sciences Indonesian Journal of Tropical and Infectious Disease Agrimeta: Agrimeta: Jurnal Pertanian Berbasis Keseimbangan Ekosistem The Indonesian Biomedical Journal Jurnal Bioteknologi & Biosains Indonesia (JBBI) WIDYA LAKSANA INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Jurnal Media Sains Indian Journal of Forensic Medicine & Toxicology Quimica : Jurnal Kimia Sains dan Terapan Seminar Ilmiah Nasional Teknologi, Sains, dan Sosial Humaniora (SINTESA) Seminar Nasional Aplikasi Iptek (SINAPTEK) Jurnal Kesehatan Terpadu JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) DEDIKASI : Jurnal Pengabdian Kepada Masyarakat Paradharma: Jurnal Aplikasi IPTEK Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Permatasari, Anak Agung Ayu Putri
Universitas Dhyana Pura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Studi Etnobotani Tanaman Obat di Desa Taro Gianyar Bali Buu, Ani Wini; Sari, Ni Kadek Yunita; Lestari, Ni Kadek Dwipayani; Permatasari, Anak Agung Ayu Putri
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 2 No. 3 (2023): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/js.v2i3.2693

Abstract

Indonesia dikenal memiliki keanekaragaman hayati flora dan fauna yang sangat beragam. Disamping itu, Indonesia juga memiliki beragam etnik, dengan berbagai kekhasan tradisinya, termasuk pengetahuan lokalnya. Salah satu pengetahuan lokal atau tradisional masyarakat Indonesia adalah pengetahuan tentang penggunaan tanaman obat Penelitian telah dilakukan terhadap masyarakat Desa Taro yang sampai saat ini masih menggunakan tanaman sebagai obat dalam mengobati berbagai jenis penyakit. Tujuan penelitian ini adalah untuk mendapatkan data jenis-jenis tanaman,mengetahui bagian bagiannya, serta menemukan cara pengelolaan dan pemanfaatannya sebagai tanaman obat tradisional oleh masyarakat Desa Taro Penelitian ini dilakukan menggunakan metode Random Sampling,berdasarkan informasi dari pengobat, dan anggota masyarakat pada masing-masing. Hasil penelitian menunjukkan bahwa di Desa Taro terdapat 28 spesis dari 23 famili tanaman yang dimanafaatkan oleh masyarakat sebagai bahan pengobatan tradisional. Cara mengolah tanaman tersebut dalam pengobatan tradisonal yaitu direbus lalu diminum, ditumbuk lalu diminum, di haluskan lalu dioleskan, di bakar lalu diminum, diparut lalu dioleskan, diparut lalu diminum, dikunyah lalu diminum. Namun masyarakat lebih sering menggunakan ramuan dengan cara direbus lalu diminum kepasien yang sakit. Bagian yang paling banyak digunakan masyarakat Desa taro sebagai bahan pengobatan pada penggunaan daun.
Uji Fitokimia dan Aktivitas Antibakteri Infusa Bonggol Pisang Batu (Musa Balbisiana Colla) terhadap Pertumbuhan Bakteri Staphylococcus Aureus Dan Escherichia Coli Rizky, Istiqomah Novia; Sumadewi, Ni Luh Utari; Permatasari, Anak Agung Ayu Putri
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 2 No. 3 (2023): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/js.v2i3.2701

Abstract

Kontaminasi bakteri Staphylococcus aureus dan Escherichia coli menyebabkan berbagai penyakit infeksi seperti pada saluran pencernaan hingga meningitis. Pemberian antibioti umum digunakan menangani penyakit infeksi. S. aureus dan E.coli telah dilaporkan memiliki resistensi tinggi terhadap antibiotik. Pisang batu (Musa balbisiana Colla) memiliki senyawa yang berpotensi menghambat pertumbuhan bakteri. Bagian buah pisang batu biasa digunakan masyarakat sebagai obat tradisional namun pada bagian bonggol belum dimanfaatkan secara maksimal. Masyarakat secara umum belum mengetahui manfaat bonggol pisang batu di bidang kesehatan, sehingga perlu dilakukan penelitian mengenai aktivitas antibakteri infusa bonggol pisang batu. Tujuan penelitian ini adalah untuk mengetahui kandungan senyawa yang dapat bertindak sebagai antibakteri dan aktivitas antibakteri infusa bonggol pisang batu (M. balbisiana Colla). Jenis penelitian ini eksperimental laboratorik secara in vitro dengan 4 perlakuan pada setiap kelompok pelarut, yaitu perlakuan menggunakan pelarut polar (etanol) dan pelarut non-polar (n-heksana) dan 2 perlakuan kelompok kontrol yaitu etanol dan n-heksana sebagai kontrol (-), gentamisin sebagai kontrol (+). Pengujian yang dilakukan meliputi uji fitokimia dan uji daya hambat antibakteri dengan metode sumuran. Hasil uji fitokimia menunjukkan infusa bonggol pisang batu (M. balbisiana Colla) pelarut etanol memiliki senyawa aktif berupa flavonoid dan tanin, pelarut n-heksana memiliki kandungan senyawa aktif berupa flavonoid, triterpenoid. Data hasil uji antibakteri menunjukkan infusa pelarut n-heksana mengasilkan daya hambat sebesar 3,16 mm terhadap bakteri S.aureus dan E.coli.
Uji Cemaran Mikrooba Dalam Sediaan Yang Distribusi Oleh PT Karya Pak Oles Tokcer Hasanah, Nina Huawatun; Permatasari, Anak Agung Ayu Putri; Deswiniyanti, Ni Wayan; Sari, Ni Kadek Yunita
JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI) Vol. 3 No. 1 (2024): JURNAL KESEHATAN, SAINS, DAN TEKNOLOGI (JAKASAKTI)
Publisher : LPPM Universitas Dhyana Pura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36002/js.v3i1.2938

Abstract

Balsam merupakan salah satu bentuk sediaan setengah padat yang digunakan sebagai obat luar. Balsam tersebut memiliki indikasi untuk meredakan nyeri pada sendi dengan memberikan sensasi hangat dan juga bisa menjadi aromaterapi. Tujuan penelitian ini adalah untuk mengetahui ada tidaknya cemaran mikroba berupa bakteri dan kapang khamir pada sediaan balsam. Penelitian ini menggunakan metode penelitian eksperimental dengan sampel berupa balsam bokashi yang diproduksi PT. Karya Pak Oles Tokcer. Data di analisis dengan deskritif kuantitatif dan dibandingkan dengan standar cemaran angka lempeng total (ALT) dan angka kapang/khamir (AKK) yang sesuai dengan peraturan BPOM No 32 tahun 2019. Hasil Angka Lempeng Total (ALT) pada produk topikal (Balsam Bokashi) menunjukan nilai pada pengenceran 101 diperoleh hasil sebesar 4 x 102, pengenceran 102 sebesar 3 x 102, pengenceran 103 sebesar 2 x 102 dan hasil Angka Kapang Khamir (AKK) pada produk topikal (Balsam Bokashi) pada pengenceran 101 diperoleh hasil sebesar 1 x 101, pengenceran 102 sebesar < x 101, pengenceran 103 sebesar, < x 101. Jadi perdasarkan penelitian ini dapat disimpulkan bahwa nilai angka lempeng total (ALT) dan angka kapang/kamir (AKK) memenuhi standar yang di tetapkan berdasarkan braturan BPOM No.32 tahun 2019.
Prevalence of bacterial contamination on seafoods products collected from traditional fish market in Bali Province during 2023: Prevalensi kontaminasi bakteri pada produk hasil ikan laut yang dikumpulkan dari pasar ikan tradisional di Provinsi Bali selama tahun 2023 Sudaryatma, Putu Eka; Wiradana, Putu Angga; Razaq, Imanuddin; Sunarsih, Ni Luh; Jatmiko, Aris; Permatasari, Anak Agung Ayu Putri; Sari, Ni Kadek Yunita; Widhiantara, I Gede; Sandhika, I Made Gde Sudyadnyana; Rosiana, I Wayan
Jurnal Pengolahan Hasil Perikanan Indonesia Vol. 28 No. 3 (2025): Jurnal Pengolahan Hasil Perikanan Indonesia 28(3)
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17844/jphpi.v28i3.61627

Abstract

Seafood provides essential nutrients beneficial for human health; however, it is highly vulnerable to harmful bacterial infections that pose significant public health risks. This research seeks to assess the prevalence of five categories of seafood obtained from various traditional fish markets in Bali Province. A total of 108 tuna samples, 78 pelagic fish samples, 37 cephalopod samples, 14 sardine samples, and 53 demersal fish samples were collected from various traditional markets in Bali Province. This research evaluated the prevalence of E. coli, coliforms, Salmonella, V. cholerae, and V. parahaemolyticus. The study revealed that the highest prevalence of E. coli, coliform, and V. parahaemolyticus contamination in tuna samples was 95 (87%), 95 (87%), and 103 (95%), respectively. The study indicated that E. coli and coliforms were present in 73 of the 78 pelagic fish samples, representing 93% contamination. Sardine samples exhibited the lowest prevalence of bacteria. All seafood samples, however, tested negative for Salmonella and V. cholera. PCR products from E. coli and V. parahaemolyticus isolates were effectively amplified for the target genes utilized in this study. Local seafood markets should adopt appropriate handling and storage practices to enhance seafood quality. This study emphasizes the significant presence of E. coli, coliforms, and V. parahaemolyticus in seafood, along with the potential health risks posed by specific strains and their antibiotic resistance.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
ISOLATION, SCREENING, AND ANTIBACTERIAL ACTIVITY OF ENDOPHYTIC FUNGI ASSOCIATED WITH Acanthus ilicifolius L. IN INHIBITING THE GROWTH OF Methicillin-Resistant Staphylococcus aureus (MRSA) Wiradana, Putu Angga; Anak Agung Ayu Putri Permatasari; I Gede Widhiantara; I Made Wisnu Adhi Putra; Ni Kadek Yunita Sari; Novaria Sari Dewi Panjaitan; Sri Puji Astuti Wahyuningsih; Mochammad Aqilah Herdiansyah
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 12 No. 2 (2025)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2025.13090

Abstract

This study examines the phytochemical composition and antibacterial activity of endophytic fungi linked to the mangrove plant A. ilicifolius against methicillin-resistant Staphylococcus aureus (MRSA) in order to determine their bioprospecting potential.  The surface-sterilized root, stem, and leaf tissues of A. ilicifolius yielded endophytic fungi. The workflow consisted of screening for viable isolates, culture under controlled circumstances, and molecular identification of the Internal Transcribed Spacer (ITS) section of fungal rDNA. Antibacterial activity was determined using agar well diffusion tests after extraction with methanol, ethyl acetate, and n-hexane solvent fractions. A total of 31 endophytic fungal isolates were recovered: 9 from roots, 15 from stems, and 7 from leaves. Among these, 5 isolates from roots (16.1%), 8 from stems (25.8%), and 5 from leaves (16.1%) exhibited inhibitory effects against MRSA. Isolate AK5, derived from root tissue, demonstrated the highest antibacterial activity and was molecularly identified as Chaetomium globosum strain NW 24 (Accession No. MN326469.1). The isolate revealed optimal growth at pH 5–6, with the maximum wet mycelial biomass (29.73 g) achieved on day 24 under shaker incubation. The methanol and ethyl acetate fractions had a considerably greater anti-MRSA efficacy than the n-hexane fraction. Phytochemical analysis of the methanolic extract indicated the presence of several bioactive components, including phenolics, tannins, flavonoids, saponins, alkaloids, and terpenoids, indicating that these constituents contribute to the reported antibacterial effects. Overall, our findings highlight the potential of A. ilicifolius-derived endophytic fungi as alternate sources of bioactive compounds for treating antibiotic-resistant infections, specifically MRSA.
Evaluation of Mangrove, Acanthus ilicifolius Aqueous Extract on Inhibition of Dengue Virus Replication: In Vitro and Molecular Docking Approach Wiradana, Putu Angga; Wiartini, Ni Wayan Ayu Wiartini; Sucipto, Teguh Hari; Herdiansyah, Mochammad Aqilah; Permatasari, Anak Agung Ayu Putri; Widhiantara, I Gede; Sari, Ni Kadek Yunita Sari; Darmanto, Win; Panjaitan, Novaria Sari Dewi Panjaitan; Kusala, Muhammad Khaliim Jati Kusala
Indonesian Journal of Tropical and Infectious Disease Vol. 13 No. 3 (2025)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v13i3.71217

Abstract

Dengue virus (DENV) infection is a worldwide health risk, presenting severe symptoms with possibly lethal consequences. The search for effective antiviral drugs against DENV has largely focused on natural products. Mangrove, Acanthus ilicifolius, has been empirically shown to exhibit various biological activities and good safety profiles.  The purpose of this investigation was to examine the antiviral activity of A. ilicifolius aqueous extract against DENV, and the way it functions of action of its bioactive components function was explored utilizing an in vitro and molecular docking method. Aqueous extract of A. ilicifolius leaves was made, and its therapeutic potential was examined using half cytotoxic concentration (CC50) and effective concentration (EC50) on Vero cells using the MTT method. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. A total of 12 ligand compounds reported to be contained in the extract were prepared for molecular docking using AutoDock Vina, followed by in silico drug-like potential.  A. ilicifolius water extract achieved the greatest Vero cell viability rate of 159.80% at a concentration of 31.25 μg/mL. A. ilicifolius extract at 100 μg/mL demonstrated the greatest DENV-3 inhibitory rate of 79.69%. The CC50 value was 78.55 μg/ml, and the EC50 value was 64.37 μg/mL.  The SI value calculated from the ratio of CC50 to EC50 was 1.22. Molecular docking identified stigmasterol, stigmasterol-3-O-β-d-glucopyranoside, and quercetin as the primary compounds with the greatest docking values (-7.9 kcal/mol, -7.6 kcal/mol, and -7.7 kcal/mol, respectively).  The findings of this study demonstrate the anti-DENV capability of the A. ilicifolius extract compound, which can limit viral multiplication via biological agents that interact with virus attachment within host cells, with an SI value of 1.22.
Co-Authors Adnyana, Gabriel Firsta Anggraeni, Ni Kadek Irma Aris Jatmiko Bagus Komang Satriyasa Buu, Ani Wini Chela Krisna Denata Desyandri Desyandri Dewi, Ni Putu Eny Sulistya Dylla Hanggaeni Dyah Puspaningrum Elok Faiqotus Zahro Ferbian Milas Siswanto Hasanah, Nina Huawatun Herdiansyah, Mochammad Aqilah I Gede Arya Sena I Gede Putu Eka Budiyasa I Gede Widhiantara I Gusti Ayu Agung Tania Devianitha I Made Endra Puniawan I Made Gde Sudyadnyana Sandhika I Made Jawi I Made Murna I Made Wisnu Adhi Putra I Wayan Putu Sutirta Yasa I Wayan Rosiana Kusala, Muhammad Khaliim Jati Kusala Luh Putu Widiastini Mochammad Aqilah Herdiansyah Ni Kadek Dwipayani Lestari NI KADEK YUNITA SARI Ni Luh Sunarsih Ni Luh Utari Sumadewi Ni Putu Widya Astuti Ni Wayan Deswiniyanti Novaria Sari Dewi Panjaitan Panjaitan, Novaria Sari Dewi Panjaitan, Novaria Sari Dewi Panjaitan Permatasari, Anak Agung Ayu Putri Permatasari Putu Angga Wiradana Putu Eka Sudaryatma PUTU INDAH LESTARI Rahmadi Prasetijo Razaq, Imanuddin Rizky, Istiqomah Novia Sari, Ni Kadek Yunita Sari Sri Puji Astuti Wahyuningsih Sumadewi, Ni Luh Utari Teguh Hari Sucipto, Teguh Hari Wiartini, Ni Wayan Ayu Wiartini Widhiantar, I Gede a Win Darmanto