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Plant Breeding Techniques in Tissue Culture to Improve the Quality of Orchids Mukhamad Su'udi; Rosida, Waki’atil; Ravitamala, El Shania; Siddiq, Arif Mohammad; Setiawan, Rendy; Setyati, Dwi; Ningrum, Asyifa Yasmin; Roziqoh, Wasiatur
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 1 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.1.116

Abstract

Conventional orchid cultivation is thought to be ineffective and time-consuming. Simple tissue culture procedures are insufficient to increase orchid quality. The need for ongoing development can be met by integrating different plant breeding strategies in orchid tissue culture, such as induction mutation, elicitor addition, and genetic transformation. The purpose of this article was to report on current improvements in the use of plant breeding techniques to orchid tissue culture to increase orchid quality. This article referenced significant scientific publications. Orchid tissue culture using mutation induction is used to develop improved variations. Giving elicitors can cause orchid plants to conserve themselves and become more resistant to diseases. The required transgene can be introduced into the genome of cultivated orchids via Agrobacterium. These advancements have the potential to revolutionize orchid cultivation. Keywords: biotechnology, orchid, tissue culture
Pengembangan Proklim Melalui Pemanfaatan Pekarangan Berkelanjutan di Desa Binaan Wonoasri, Kecamatan Tempurejo, Kabupaten Jember Sulistiyowati, Hari; Hasanah, Ela Apriliyanti; Ratnasari, Tri; Dewi, Nilasari; Siddiq, Arif Mohammad; Kurnianto, Agung Sih; Yulianto, Roni; Widodo, Nur
Reswara: Jurnal Pengabdian Kepada Masyarakat Vol 5, No 2 (2024)
Publisher : Universitas Dharmawangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46576/rjpkm.v5i2.4112

Abstract

Desa Wonoasri memiliki potensi dalam pengembangan program kampung Iklim (ProKlim) yang salah satunya pada aspek pemanfaatan pekarangan masyarakat. Oleh karena itu kegiatan pengabdian ini bertujuan untuk mengembangkan Desa ProKlim melalui pemanfaatan pekarangan berkelanjutan di Desa binaan Wonoasri, Kecamatan Tempurejo, Kabupaten Jember. Metode pendekatan yang digunakan adalah pendekatan personal dan kelompok melalui sosialisasi dan studi banding, Focus Group Discussion (FGD), dan pendampingan serta demonstrasi. Tolok ukur keberhasilan pengembangan Proklim adalah respon positif Masyarakat dan perangkat desa terhadap keseluruhan kegiatan yang akan dilakukan. Hasil dari kegiatan pengabdian ini adalah respon positif dari masyarakat terhadap ProKlim yang cukup tinggi dengan keterlibatan 35 masyarakat yang mewakili berbagai kalangan (anak muda, ibu-ibu PKK, bapak-bapak, LMDHK, perangkat desa) dan diskusi interaktif selama kegiatan. Selain itu tiga kolam ikan lele model terpal berkerangka besi diameter 3m dengan masing-masing 1000 bibit ikan lele telah direalisasikan. Demikian juga halnya dengan realisasi 6 rangka besi vertikultur tanaman sayuran yang meliputi tomat, terong, dan pakcoi. Aktivitas diversifikasi produk mangga juga sudah dilaksanakan yaitu memberikan teori dan praktik dalam diversifikasi olahan buah mangga menjadi sirup, puding, kue bolu, dan manisan. Kesimpulan dari kegiatan pengabdian ini yaitu pengembangan ProKlim di Desa Wonoasri berhasil dilakukan berdasarkan respon positif masyarakat, realisasi kolam ikan dan vertikultur, dan diversifikasi produk buah mangga. Selanjutnya program ini juga mendapat dukungan sepenuhnya dari Kepala Desa Wonoasri yang akan melanjutkan program Desa ProKlim.
Keanekaragaman Jenis Kupu-Kupu (Lepidoptera: Rhopalocera) Di Zona Rehabilitasi Blok Bonangan Resort Wonoasri Taman Nasional Meru Betiri Setiawan, Rendy; Siddiq, Arif Mohammad
Biosel Biology Science and Education Vol. 12 No. 1 (2023): BIOSEL (Biology Science and Education: Jurnal Penelitian Sains dan Pendidikan)
Publisher : INSTITUT AGAMA ISLAM NEGERI AMBON

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33477/bs.v12i1.4276

Abstract

Kupu-kupu termasuk serangga ordo Lepidoptera (subordo Rhopalocera) yang berperan penting bagi keseimbangan ekosistem. Peran utama kupu-kupu yaitu sebagai konsumen primer dan serangga polinator. Penelitian ini bertujuan untuk menentukan keanekaragaman jenis kupu-kupu di zona rehabilitasi Blok Bonangan, Resort Wonoasri TNMB. Metode pengambilan spesimen menggunakan metode jelajah dengan teknik ayunan tidak berpola.Hasil penelitian ditemukan 12 jenis kupu-kupu (Lepidoptera: Rhopalocera). Nilai indeks keanekaragaman jenis kupu-kupu tergolong sedang yaitu 2,07. Kesimpulan dari penelitian ini yaitu ditemukan sebanyak 235 individu kupu-kupu yang tergolong dalam 12 jenis, 3 famili dan 11 genus antara lain yaitu Neptis sp., Junonia sp., Euploea sp., Hypolimnas sp., Orsotriaena sp., Mycalesis sp., Ypthima sp.,Leptosia sp., Eurema sp., Jamides sp., Appias sp. (1)., dan Appias sp. (2).Nilai indeks keanekaragaman jenis kupu-kupu (Lepidoptera: Rhopalocera) di zona rehabilitasi Blok Bonangan Resort Wonoasri TNMB tergolong sedang yaitu 2,07.
The Diversity of Waterbirds Species in Jatipapak Mangrove Ecosystem at Kucur Resort, Alas Purwo National Park, East Java Siddiq, Arif Mohammad; Sulistiyowati, Hari; Wimbaningrum, Retno; Setiawan, Rendy; Qisti, Nissaul; Supriadi, Dudun
Biosfer: Jurnal Tadris Biologi Vol 14 No 2 (2023): Biosfer: Jurnal Tadris Biologi
Publisher : UNIVERSITAS ISLAM NEGERI RADEN INTAN LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/biosfer.v14i2.17185

Abstract

The study aims to determine the composition and diversity of waterbirds species in the Jatipapak mangrove ecosystem at Alas Purwo National Park (APNP). This research was conducted on September-October 2020 with terrestrial and marine surveys in three stations. Data collection includes the waterbirds species and the number of individuals of each species. Data analysis used species composition and diversity index (Shannon Wiener=H’). The results of the study obtained the existence of 837 individuals waterbirds consisting of seven families, 16 genera, and 23 species. The waterbird communities in this area are composed of 11 species of resident birds and 12 species of migratory birds. The diversity index of waterbird species obtained is 2.31 which means moderate level. Based on the conservation status, there were 22 species of waterbirds that were classified as least concern (LC) and one species namely the Lesser Adjutant (Leptoptilos javanicus) which was included in the Vulnerable (VU) category.ABSTRAK: Penelitian bertujuan untuk menentukan komposisi dan keanekaragaman spesies burung air di ekosistem mangrove Jatipapak di Taman Nasional Alas Purwo (TNAP). Penelitian ini dilakukan pada bulan September-Oktober 2020 dengan survei darat dan laut di tiga stasiun. Data yang dicatat meliputi spesies burung air dan jumlah individu setiap jenis. Analisis data menggunakan penentuan komposisi spesies dan indeks keanekaragaman jenis Shannon Wiener (H’). Hasil penelitian diperoleh 837 individu burung air yang terdiri dari 7 famili, 16 genus, dan 23 spesies. Komunitas burung air di kawasan ini terdiri dari 11 spesies burung penetap dan 12 spesies burung migran. Nilai indeks keanekaragaman jenis burung air yang diperoleh adalah 2,31 yang berarti dalam kategori sedang. Berdasarkan status konservasi, terdapat 22 spesies burung air yang tergolong least concern (LC) dan satu spesies yaitu bangau tongtong (Leptoptilos javanicus) yang masuk dalam kategori Vulnerable (VU).
Assessing The Javan Leopard Presence and Prey Abundance: Insights from Camera Trap Surveys in Meru Betiri National Park East Java Indonesia Kholiq, Nur; Sulistyowati, Hari; Tabah Wibisono, Hariyo; Asmoro Lelono; Retno Wimbaningrum; Sattya Arimurti; Arif Mohammad Siddiq; Adi Sucipto
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 2 (2025)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i2.53715

Abstract

The use of incomplete or imbalanced data in ecological modelling and conservation planning can lead to inaccurate predictions due to limitations in detection methods. Therefore, obtaining comprehensive and precise empirical data on species presence and availability, particularly prey species is essential. This study aims to: (1) identify the presence of Javan leopards and their potential prey species, and (2) estimate the relative abundance indices (RAI) of each prey species. Using camera trap data from the Meru Betiri National Park (MBNP) survey conducted in 2024, we recorded 24 species, including the Javan leopard, and identified seventh potential prey species. We estimated relative abundance of each potential prey species was determined by dividing the number of individuals recorded in independent photos or videos by the total number of trap days and multiplied by 100. The result of RAI analysis indicated that the most abundant prey species in the study area was the long-tailed macaque, with an RAI value of 44.16 individuals per 100 trap-days, banteng was the least frequently detected species, appearing in only three independent images, with an RAI value of 0.23. The presence of Javan leopards, a charismatic flagship species, highlights the ecological significance of this area and provides valuable data for biodiversity management, serving as a foundation for future research and conservation efforts.
Penguatan Citizen Science pada Lembaga Masyarakat Desa Hutan Konservasi Wonomulyo dalam Menginisiasi Pembentukan Ekowisata Birdwatching Siddiq, Arif Mohammad; Sulistiyowati, Hari; Ratnasari, Tri; Dewi, Nilasari; Cahyono, Haris
Jurnal Pengabdian Kepada Masyarakat (JPKM) TABIKPUN Vol. 5 No. 1 (2024)
Publisher : Faculty of Mathematics and Natural Sciences - Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jpkmt.v5i1.133

Abstract

Lembaga Masyarakat Desa Hutan Konservasi (LMDHK) Wonomulyo merupakan kelompok masyarakat yang fokus pada kegiatan konservasi di Taman Nasional Meru Betiri (TNMB). Dalam upaya peningkatan skill dan kompetensi kegiatan konservasi tersebut, maka tim pengabdian Universitas Jember melakukan kegiatan pengabdian kepada LMDHK Wonomulyo dengan tema penguatan “Citizen Science” dalam menginisiasi pembentukan ekowisata “birdwatching”. Kegiatan ini dilakukan pada bulan Juli-September 2023 dengan beberapa kegiatan yang meliputi sosialisasi kegiatan, peningkatan pemahaman, dan implementasi lapang. Berdasarkan kegiatan pengabdian yang telah dilakukan, pengurus LMDHK telah mendapatkan skill pengamatan burung berdasarkan morfologi, identifikasi menggunakan buku panduan, dan rekapitulasi data. Keragaman jenis burung yang ditemukan di zona rehabilitasi Resort Wonoasri TNMB memiliki potensi sebagai pengembangan paket wisata “birdwatching”.
PENGEMBANGAN PERTANIAN RENDAH EMISI SEBAGAI UPAYA PENGUATAN PROKLIM DI DESA WONOASRI KECAMATAN TEMPUREJO KABUPATEN JEMBER. Ratnasari, Tri; Hari Setiyowati; Nilasari Dewi; Arif Mohammad Siddiq
Jurnal Pengabdian Magister Pendidikan IPA Vol 8 No 4 (2025): Oktober-Desember 2025
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jpmpi.v8i4.10738

Abstract

Abstract Climate change is a global phenomenon characterized by changes in patterns, intensity, or shifts in key climate parameters that impact ecosystems and human life. The effects of climate change are increasingly being felt, necessitating effective mitigation and adaptation efforts. The government has initiated the Climate Village Program (ProKlim) as an integrated strategy to reduce the impacts of climate change through local actions. Wonoasri Village, which directly borders the Meru Betiri National Park (TNMB), has implemented various environmental mitigation strategies, such as the development of alley forests and home gardens. However, mitigation efforts in the agricultural sector remain limited. The agricultural system applied in Wonoasri Village still largely relies on the use of chemical inputs, including fertilizers and pesticides, to support plant growth and resilience. The use of these chemicals has led to ecosystem degradation, as evidenced by the decline in populations of pollinating insects, birds, and other beneficial insects. Moreover, the excessive use of chemical fertilizers and pesticides has caused environmental damage, such as soil hardening and increased plant resistance to pests and diseases. In response to these issues, TNMB has collaborated with farmer groups through the Community-Based Forest Conservation Institution (LMDHK) to develop a low-emission agricultural system. Since 2017, initiatives have been implemented to reduce the use of chemical fertilizers by replacing them with solid organic fertilizers and liquid organic fertilizers (POC) derived from cattle and goat manure waste. This community service program aims to equip LMDHK members with the skills to process green waste into organic nutrient sources, particularly nitrogen (N), phosphorus (P), and potassium (K), which serve as organic fertilizers. Additionally, they are trained in producing botanical pesticides from green waste as an alternative to chemical pesticides for controlling plant pests and diseases. The primary goal of this initiative is to establish a low-emission agricultural system through the optimization of green waste utilization as a plant nutrient source and botanical pesticide. The implementation of this program is expected to help farmers reduce their dependence on chemical fertilizers and pesticides, thereby lowering agricultural production costs. Furthermore, this effort contributes to reducing greenhouse gas emissions through a sustainable agricultural system. The program is carried out through a series of activities, including program socialization, workshops on the production of organic NPK fertilizers and botanical pesticides, as well as monitoring and evaluation. The successful implementation of this program has resulted in organic fertilizer and botanical pesticide products that can be utilized by farmers as a concrete step towards achieving more environmentally friendly and sustainable agriculture. Keywords: ProKlim, Low-Emission Agriculture, Natural NPK, Botanical Pesticide
The Diversity of Birds in Mangrove Forest at Biosite Pangpang Bay, Ijen Geopark Siddiq, Arif Mohammad; Wimbaningrum, Retno; Sulistiyowati, Hari; Setiawan, Rendy; Febrianti, Silvia Dwi; Sabila, Firman Syauqi Nur
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44165

Abstract

The Biosite Pangpang Bay Ijen Geopark (BPBIG) has a high potential biodiversity value outside the conservation area, particularly in the mangrove forest. Namun informasi keragaman ini masih belum sepenuhnya lengkap. Sehingga penelitian ini bertujuan untuk menentukan keragaman jenis burung di hutan mangrove BPBIG. The research was conducted on October 2023 using the point count method at six observation points in the mangrove forest. Data analysis was carried out in two approaches, i.e., species composition and species diversity index using Shannon Wiener index (H’). We recorded 39 bird species, consisting of 32 genera and 21 families, in the mangrove forest of BPBIG. There is one bird species that has important conservation status based on the IUCN Red List and National Regulation, namely L. javanicus (a vulnerable and protected species). Furthermore, there are also three migratory birds (T. sanctus, N. phaeopus, and A. hypoleucos) that use the mangrove forest of BPBIG as stopover sites during migration periods. The results of the Shannon Wiener index showed that the bird diversity in the BPBIG mangrove ecosystem is in the medium category (H' = 2.02). Finally, based on these results, it can be concluded that the bird species in the mangrove forest of BPBIG are quite diverse.
Community Structure of Avifauna in The Rehabilitation Zone at Wonoasri Resort, Meru Betiri National Park Siddiq, Arif Mohammad; Sulistiyowati, Hari; Ulaa, Munaa Aqidatul; Ulum, Fuad Bahrul; Fadri, Firda
Jurnal Biodjati Vol 9 No 1 (2024): May
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v9i1.30033

Abstract

The rehabilitation zone of Meru Betiri National Park (MBNP) needs to be measured in relation to the success of ecosystem restoration. It can be used as a bioindicator, such as the bird community structure, hence their sensitivity to environmental changes. Therefore, the aims of this study are to determine the community structure of avifauna in the rehabilitation zone at Wonoasri Resort, MBNP including bird diversity, richness, dominance, feeding guilds, and their correlation with forest cover. The research was conducted in January 2023 in the Bonangan Block at three observation points, namely low vegetation cover, medium vegetation cover, and high vegetation cover. The ecological data, such as species and abundance, were collected using the point count method, while additional information related to conservation statuses were collected by web browsing on the IUCN Red List, the CITES appendix, and Indonesian government regulation (LHK No.106/2018). Data analysis used the dominance index, species diversity index (Shannon Wiener), and evaluated for statistical data using a Kruskal-Wallis (KW) test in R version 3.2.1. We recorded 38 bird species belonging to 31 genera and 21 families during this study. Among the feeding guilds, the insectivorous birds (14 species) were recorded as the higher species richness, followed by omnivorous (eight species), frugivorous (five species), granivorous (five species), carnivorous (four species), and nectarivorous (two species). The Kruskal-Wallis test shows there is a significant correlation among the feeding guilds (value of 11.644, with p-value = 0.040). Furthermore, referring to species richness, high vegetation cover areas have the highest species richness (30 species) compared to medium vegetation cover areas (27 species) and low vegetation cover areas (26 species). The dominance of avifauna species at this location falls into the low category (C = 0.07). According to the diversity index, avifauna in Bonangan Block is classified as a high category (H'=3.01).
Implementation of Botanical Pesticides and Yellow Traps for Pest Control in Farmer Groups of Gombolirang Village, Kabat Sub-district, Banyuwangi Regency Muhammad Dafa Riansyah; Nadia Dwi Safitri; Diah Ayuk Wulandari; Nazala Zaikumar Elfa Rizqi; Gita Puspita Sari; Ira Ika Puspitasari; Fiky Binti Zakiyah; Audina Rahma Sari; Fernanda Bagus Prasetya; Muhammad Doni Gunarso; Arif Mohammad Siddiq
Jurnal Inovasi Sains dan Teknologi Untuk Masyarakat Vol. 3 No. 2 (2025): November
Publisher : Faculty of Mathematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/instem.v3i2.6

Abstract

The community service program conducted by the Community Service (KKN) Team of the University of Jember in Gombolirang Village provided practical solutions to agricultural challenges, particularly in pest and disease control. The program focused on introducing environmentally friendly alternatives, including the use of botanical pesticides made from easily accessible plant-based ingredients such as lemongrass, maja fruit, and garlic, as well as the installation of yellow traps. Activities carried out in August 2025 included field observation, farmer interviews, extension sessions, demonstrations on the production of botanical pesticides and yellow traps, along with interactive discussions with farmer groups from five hamlets (Krajan, Kertosari, Cungking, Sempu, and Ancar). Data were collected through direct observation and evaluation of the community’s response to the introduced innovations. The implementation of this program showed that farmers in Gombolirang Village were highly enthusiastic and successfully gained practical skills in producing botanical pesticides and applying yellow traps as cost-effective and environmentally friendly pest control methods. These technologies were considered effective in reducing pest populations without disrupting the rice field ecosystem. Most participants also expressed their willingness to independently apply these methods in the next planting season. Thus, this program contributed to enhancing farmers’ capacity and encouraging the adoption of sustainable agricultural practices at the local level.
Co-Authors Abdillah Baraas Adi Sucipto, Adi Afina Aninnas Agung Sih Kurnianto Agung Sih Kurnianto Agung Sih Kurnianto Ali Badrudin Ananda, Mia Bella Dwy Ananda, Rizky Putra Anugrah Bahtiar Triantono Aprilia Palupi, Annisaa Arif Pratiwi Audina Rahma Sari Banun Kusumawardani Baraas, Abdillah Cahyono, Haris Dewi Setyari, Agustina Dewi, Nilasari Diah Ayuk Wulandari Dwi Setyati Dwi Susanto, Miftha Amilia Dwi Wardatul Rizkiah Fadri, Firda Febrianti, Silvia Dwi Fernanda Bagus Prasetya Fiky Binti Zakiyah Firda Fadri Firdaus, Anggun Sausan Firmansyah, Puji Fuad Bahrul Ulum Gita Puspita Sari Hanif Roudhatul Firdausiyah Hari Setiyowati Hari Sulistiyowati Hari Sulistyowati Hasanah, Ela Apriliyanti Imaniar, Raisa Indra Nurtjahjaningtyas Iqbal Setiawan Saputra Ira Ika Puspitasari Jasmine, Nur Ainna Fairuz Kholiq, Nur Khowatini, Husnul Lelono, Asmoro Mega Fatimah Rosana Mita Yuni Aditiya Muhammad Dafa Riansyah Muhammad Doni Gunarso Muhammad Kurniawan Muhammad Kurniawan Mukhamad Su'udi Munaa Aqidatul Ulaa Nadia Dwi Safitri Nazala Zaikumar Elfa Rizqi Nilasari Dewi Nilasari Dewi Nindha Ayu Berlianti Nindy Agusti Wulansari Ningrum, Asyifa Yasmin Nur Kholiq Nur Widodo Nursalamah, Amanda Dwi Pradana, Yuan Zulia Pratiwi, Arif Puji Firmansyah Puji Firmansyah Qisti, Nissaul Rani, Cynthia Ratnasari, Tri Ravitamala, El Shania Rendy Setiawan Rizkiah, Dwi Wardatul Rosida, Waki’atil Roziqoh, Wasiatur Sabila, Firman Syauqi Nur Samsuri Samsuri Sattya Arimurti Setiawan, Rendy Sudarmadji Sudarmadji Sudarmadji Sudarmadji Sulistiyowati, Hari Supriadi, Dudun Tabah Wibisono, Hariyo Tecky Indriana Ulaa, Munaa Aqidatul Wahono, Nanang Dwi Wati, Dewi Erna Wimbaningrum, Retno Wulansari, Nindy Agusti Yennita Dwi April Liana Yulianto, Roni Zahra, Anis Syavira Avivatu