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BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan)
Published by Universitas Medan Area
ISSN : 25975269     EISSN : 2356458X     DOI : 10.31289
Biolink focuses on the publication of the results of scientific research related to fields. This article is published in the internal and external academic community of the University of Medan Area (UMA) especially in the field of Biology. Biolink publishes scientific articles in the scope of biology that includes environmental biology, industrial biology and health biology. Published articles are the results of research articles, studies or critical and comprehensive scientific studies on important issues related to the field of biology.
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Articles 378 Documents
ECO-FRIENDLY INNOVATION: POTENTIAL OF RED DRAGON FRUIT PEEL EXTRACT IN SILVER NANOPARTICLE BIOSYNTHESIS Dwi Puspita, Alfiana; Adhila, Ghina; Sari, Della Puspita; Wicaksono, Sigit Candra; Fahriati , Zahira
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.13331

Abstract

East Kalimantan is one of the regions that cultivates red dragon fruit (Hylocereus polyrhizus) in large quantities due to its promising health benefits. The peel of red dragon fruit has antioxidant activity comparable to its flesh, yet it is rarely utilized and often discarded as organic waste. This study aims to explore the potential of water and ethanol extracts from red dragon fruit peel as antioxidant sources that can serve as natural reducing and stabilizing agents in the biosynthesis of silver nanoparticles (AgNPs). Extraction using water and ethanol solvents was conducted to analyze the antioxidant compounds in the peel. The extract with the strongest antioxidant activity was used for AgNP biosynthesis, and the absorption peak was observed using UV-Vis spectrophotometry at a specific wavelength of 400–450 nm, which is characteristic of AgNP surface plasmon resonance. The results showed that the ethanol extract exhibited stronger antioxidant potential based on its lower IC₅₀ value and functioned effectively as a reducing and stabilizing agent at synthesis temperatures of 40–50°C, without degrading the extract's secondary metabolites. These findings support the utilization of red dragon fruit peel as a sustainable and eco-friendly raw material for the development of health-related products.
BIODIVERSITY OF ARACEAE IN MALABAR CITY FOREST, MALANG CITY, EAST JAVA Aulia Azahra, Rahmania; Syamsuddin, Imam; Asyaun Niqris, Adelliana; Relia Fitrah, Aura; Nadia, Intan; Setyoningrum, Nadia; Chairani Fatimah, Nina; Fardhani, Indra; Qomaliyah, Eka Nurul
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.13690

Abstract

Indonesia, located on the equator with over 17,000 islands, is among the world's most biodiverse countries. Malabar City Forest in Malang serves as both a green space and a habitat for various plant species, including members of the Araceae family. Despite their roles in microclimate regulation, air purification, and soil stabilization, Araceae diversity in urban areas remains underexplored. This study aimed to identify and document Araceae species in Malabar City Forest and assess their ecological roles. Using an exploratory survey and cruise sampling, species were recorded based on their morphology. Field data were collected through direct observation aided by smartphone-based identification tools and validated with online taxonomic resources and literatures. Seven species were identified: Dieffenbachia seguine (Jacq.) Schott, Epipremnum aureum (Linden & André) G.S Bunting, Alocasia plumbea Van Houtte, Syngonium angustatum Schott, Syngonium podophyllum Schott, Epipremnum pinnatum (L.) Engl., and Typhonium trilobatum Schott, representing six genera. Their presence highlights adaptability to urban environments and potential as bioindicators. Future research should assess their ecological resilience and interactions. Local conservation through education, labeling, and habitat preservation can support biodiversity awareness and sustainable urban planning.
POTENTIAL FORMATION OF ACID MINE DRAINAGE BASED ON THE GEOCHEMISTRY OF GOLD ORE ROCKS Aminah, Siti; Haeruddin, Haeruddin; Suparno, Fanteri Aji Dharma; Irawan, Januar Fery
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14182

Abstract

Open pit mining activities can have many impacts on the environment, such as the formation of mine pits containing acid mine drainage. This requires proper and correct handling so as not to harm the environment. The acidity of mine drainage can vary greatly depending on the quantity and type of minerals it contains. The objectives of this research are: to identify and determine the rock materials that have the potential to generate acid mine drainage (AMD) as Potentially Acid Forming (PAF) rocks and Non-Acid Forming (NAF) rocks. The research began with sample preparation, mineralogical analysis, AAS, static testing, and XRF testing. The research results indicate that the samples are primarily composed of quartz, with some hematite and goethite grains observed as fine, curved pore fillings. Gold (Au) is found within the mineral electrum (AuAg). The minerals present in the gold ore rocks are predominantly oxides, consisting of 84.7% SiO2, 8.23% Fe2O3, and other components. The rock samples are classified as NAF, as indicated by the paste pH analysis results of 7.05 and 6.94, total sulfur analysis values of 0.37% and 6.43%, and NAPP values of -5.1 and -3.5.
ISOLATION AND SELECTION OF ENDOPHYTE FUNGI FROM THE ANDALIMAN PLANT (Zanthoxylum acanthopodium DC) AS ANTIFUNGAL Idramsa, Idramsa; Ayu, Nur
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14366

Abstract

Dermatophytosis (Ringworm) is a disease caused by dermatophyte fungal infection that attacks the epidermis (keratin) tissue such as skin, nails and hair in humans. Antibiotics as a drug for fungal infections that are often used by the public are known to have side effects, so that alternative drugs are needed that are relatively safe to use. Endophytic microbes found in plants are known to be an alternative natural medicine that does not have side effects, because they contain bioactive compounds. This study aims to isolate, select and identify endophytic fungi in andaliman plants (Zanthoxylum acanthopodium DC) and determine their potential as antifungals against dermatophytosis fungi. Exploration methods were used, molecular analysis using marker genes Internal Transcribed Spacer (ITS) and antifungal activity tests (Dual culture) against Tricophyton rubrum and Tricophyton mentagrophytes as pathogenic test fungi. The results showed that endophytic fungus from the species Diaporthe phaseolorum (Isolate EF128). has very strong antifungal activity against the test fungus Tricophyton rubrum and is strong against Tricophyton mentagrophytes (isolate IJE D3). so it has the potential as an antifungal.
CARBON ABSORPTION POTENTIAL IN MANGROVE ECOSYSTEM IN BORONGKALUKUA, MAROS REGENCY, SOUTH SULAWESI, INDONESIA Salwa, Sausan As; Rukminasari, Nita; Alam, Jamaluddin Fitrah; Rahim, Sri Wahyuni
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14394

Abstract

Mangrove ecosystems are essential in mitigating climate change through Carbon storage in sediments. This study aims to analyze the bulk density values, carbon concentration, and Carbon storage and absorption in mangrove sediments in Borongkalukua, Maros Regency. Sampling was carried out at five sediment depths (0-10 cm, 10-20 cm, 20-30 cm, 30-40 cm, and 40-50 cm) using the Loss on Ignition (LOI) method and analyzed statistically using one-way and two-way ANOVA. The results showed an average bulk density value of 0.33 g/cm³, with the highest value at a depth of 30-40 cm (0.28 g/cm³). Furthermore, the average Carbon concentration was 11.37%, with the highest value at a depth of 40-50 cm (20.50%) and the lowest at 0-10 cm (1.98%), with no significant differences between depths (p-value > 0.05). Meanwhile, Carbon storage in sediment reached 2,423.77 tons/ha. Total absorption reaching 8,895.23 tons/ha. In conclusion,  sediment depth affects carbon accumulation, with higher carbon content in deeper layers due to the sedimentation process and minimal decomposition. This study emphasizes the importance of mangrove conservation in supporting Carbon storage and climate change mitigation.
EFFECT OF SYZYGIUM CUMINI AQUEOUS EXTRACT ON CARBON TETRACHLORIDE-INDUCED TESTICULAR DAMAGE IN MICE: A HISTOMORPHOMETRY Susilowati, Rina Priastini; Santoso, Adit Widodo; Sabini, Jasmine Harumi
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14506

Abstract

The juwet fruit (Syzygium cumini) is a type of fruit commonly found in Indonesia and is known to contain high levels of antioxidant compounds, potential for protecting reproductive organs from oxidative damage. This study aims to investigate the effects of juwet fruit in protecting the testes from damage caused by CCl4 toxicity in mice. A total of 30 mice were used and divided into six groups: distilled water, 12.5 mg/mL CCl₄, and S. cumini extract at doses of 5, 10, 20, and 40 mg/mL for three weeks.  Parameters assessed included body weight, serum testosterone levels, sperm count, and histomorphometry (seminiferous tubule diameter, germinal epithelium height, and spermatogenic cell counts). The results showed that CCl₄ significantly reduced body weight, the number of spermatogenic cells in the seminiferous tubules, seminiferous tubule diameter, germ cell height, and serum testosterone levels in the mice. The treatment groups receiving extract of juwet fruit at doses of 20 mg/mL and 40 mg/mL showed improvements in testicular function that were not significantly different from the distilled water group. In conclusion, reproductive toxicity induced by CCl₄ in male mice can be ameliorated by administering aqueous extract of juwet fruit at doses of 20 mg/mL and 40 mg/mL.
CHARACTERISTICS AND POTENTIAL OF MICROPLASTIC DEGRADING BACTERIA FROM LOCAL MICROORGANISMS OF LERI ARUK FROM BANGKA BELITUNG Desi, Desi; Syafiqo, Syafira Auliani; Olieviani, Marisa; Sari, Maya; Swandi, Monica Kharisma
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14520

Abstract

One of the efforts to reduce the impact of microplastic pollution is through local microorganisms (MOL). Practical and applicable MOL can be obtained from local materials such as leri aruk Bangka Belitung. This study aims to test the characteristics of various MOL formulations from leri aruk typical of Bangka Belitung as candidates for microplastic degradation agents and to characterize the bacteria. This study was conducted in several stages, namely sampling, making various MOL formulations, testing MOL based on physical, chemical and biological properties, isolation and characterization of microplastic degrading bacteria and testing the ability of bacteria to degrade microplastics. The results of the study obtained the best formulation results based on physical, chemical and biological properties, namely in formulation 2. The isolation results obtained 4 isolates that have the potential to degrade microplastics, namely isolates B13, B18, B19 and B20.Morphological and biochemical characterization of bacteria showed that isolates B13 and B19 were LactoBacillus, isolate B18 was Bacillus and isolate B20 was Escherichia. The consortium of 4 isolates can degrade microplastics by 7.14% for 1 week on LDPE plastic.
EVALUATION OF THE CAPABILITY OF IMMOBILIZED CHLORELLA VULGARIS (BEIJERINCK, 1890) IN ALGINATE BEAD MATRIX AS A BIOADSORBENT FOR HEAVY METAL ZINC (Zn) Putranto, Aldo Ferio; Hazmi, Nubli; Hariani, Nova; Arif, Muhammad Fauzi
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14615

Abstract

Bioremediation intends to use living organisms to remove or decompose pollutants, using their ability to metabolize or accumulate hazardous substances, thus reducing toxicity. This study aims to compare the removal efficiency of free suspended Chlorella vulgaris and immobilized Chlorella vulgaris. Monoculture isolates of Chlorella vulgaris were cultured in an aqueous medium with KW21 fertilizer for approximately 12 days until reaching the stationary phase. The biomass was harvested, dried, and separated. A ZnCl₂ solution was prepared with 2, 4, and 8 ppm working concentrations. The biosorption efficiency was calculated to evaluate the biosorption capacity. The results indicated that the removal efficiency of free suspended C. vulgaris was 14.73%, 6.76%, and 10.22% for the respective concentrations. Immobilized C. vulgaris exhibited significantly higher removal efficiencies of 73.79%, 77.87%, and 83.80%. This study shows that immobilizeed C. vulgaris have a higher removal efficiency value than free suspended C. vulgaris cells because they can withstand heavy metal toxicity better. This study implicates that the treatment of alginate beads increases the stability and effectiveness of biosorption, and makes this method a preferred approach for heavy metal bioremediation applications in aquatic environments.
POLLEN MORPHOLOGICAL DIVERSITY OF ASTERACEAE SPECIES IN THE TADULAKO UNIVERSITY CAMPUS AREA Budiono, Yudhistira Kristinov; Rasyiid, Mustafid; Musa, Khairul; Iqbal, Moh.
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.14679

Abstract

This study aims to explore the morpho-anatomical diversity of pollen from various plant species belonging to the Asteraceae family within the Tadulako University area. Flower samples were collected using an exploration method and prepared for palynological analysis using a modified acetolysis technique. Six Asteraceae species were identified: Tridax procumbens L., Cyanthillium cinereum (L.) H.Rob., Synedrella nodiflora (L.) Gaertn., Eleutheranthera ruderalis (Sw.) Sch.Bip., Porophyllum ruderale (Jacq.) Cass., and Chromolaena odorata (L.) R.M.King & H.Rob. The study revealed significant differences in pollen morphology, particularly in shape (spheroidal), aperture number (3–4 colporates), ornamentation (echinate vs. lophate), and size. Pollen size ranged from 21.29 µm to 35.66 µm (equatorial diameter) and wall thickness ranged from 2.16 µm to 4.82 µm. These variations serve as reliable taxonomic markers for distinguishing species at both genus and species levels. This research provides valuable insights into the biodiversity and evolutionary adaptations of the Asteraceae family and supports the use of palynological data in systematic botany.
IDENTIFICATION OF PLANT GROWTH PROMOTING PROPERTIES OF BACTERIA ISOLATED FROM WATER HYACINTH (Eichhornia Crassipes) ROOTS Putri, Theodora Mega; Tambun, Rizka Arta Rossa; Martgrita, Merry Meryam
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol. 12 No. 1 (2025): Biolink August
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31289/biolink.v12i1.15121

Abstract

Plant growth-promoting bacteria (PGPB) play an important role in enhancing plant growth and productivity through various biological mechanisms, such as phosphate solubilization and nitrogen fixation. This study aims to isolate and identify PGPB from the roots of water hyacinth (Eichhornia Crassipes) growing in Lake Toba, and to evaluate its potential in supporting the growth of lettuce (Lactuca sativa). Three rhizosphere bacteria were obtained and identified morphologically and biochemically as Bacillus thuringiensis (EGR1), Bacillus megaterium (EGR2), and Azotobacter vinelandii (EGR3). All three isolates showed the ability to solubilize phosphate, while only EGR3 was able to fix nitrogen. Bioassay tests using lettuce showed that EGR2 increased plant height, while EGR3 significantly increased fresh weight, number of leaves, and length of roots. These findings indicate the potential of EGR3 as a promising natural biofertilizer candidate to support sustainable agriculture.