cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
,
INDONESIA
Molecular and Cellular Biomedical Sciences (MCBS)
Published by Cell and Biopharmaceutical Institute
ISSN : 25274384     EISSN : 25273442     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience
Molecular and Cellular Biomedical Sciences (MCBS) has been published by Cell and BioPharmaceutical Institute (CBPI), a biannually published scientific journal, is an open access, peer-reviewed journal that supports all topics in Biology, Pathology, Pharmacology, Biochemistry, Histology and Biomedicine in the aspect of molecular and cellular.
Arjuna Subject : -
Articles 174 Documents
 6   7   8   9   10 
Correlation between Protein Intake, Fat Free Mass, and Total Lymphocyte Count with Quality of Life in Pulmonary Tuberculosis Patients Undergoing Intensive Phase Treatment in Pekanbaru, Riau Province Dewi Krisna Yunda; Fiastuti Witjaksono; Fariz Nurwidya
Molecular and Cellular Biomedical Sciences Vol 4, No 3 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i3.144

Abstract

Background: Malnutrition and tuberculosis (TB) have a bidirectional relationship, which interacts between each other. In chronic infection, there is an imbalance between protein degradation and protein synthesis which marked with the loss of fat-free mass (FFM). Malnutrition can cause the atrophy of the thymus gland resulted in the reduction of lymphocyte production. Malnutrition in TB patients will reduce the quality of life. On the other hand, a good quality of life will increase the treatment success rate and decrease the risk of morbidity and mortality. Materials and Methods: A cross-sectional study was conducted in 12 primary health centers (PHC) chosen randomly from 23 PHC in Pekanbaru, Riau Province. The random selection was performed by using simple random sampling (random number generator). Data were collected from May until July 2019. Samples were selected using a consecutive sampling method and 72 subjects fulfilled all research criteria. The interview was used to collect basic characteristic data, dietary intake data, and quality of life score. Anthropometric measurement (body weight, body height, and FFM) and laboratory examination (total lymphocyte count) were done.Results: Research showed median age subjects was 33 years old (18-59 years old). Most of the subjects were male (56.9%), had a middle level of education, low income, were active smokers with underweight nutritional status. More than 50% of subjects had low protein intake, low fat-free mass, normal lymphocyte count and had a good quality of life.Conclusion: There was a statistically significant correlation between fat-free mass with PCS (r=0.239, p=0.044), but not for protein intake and total lymphocyte count.Keywords: fat-free mass, protein, quality of life, total lymphocyte count, tuberculosis
Serum Leptin Concentration is Correlated to Insulin Resistance in Polycystic Ovary Syndrome (PCOS) Patients Arsyi Adliah Anwar; Nusratuddin Abdullah; Andi Nursanty Padjalangi; Firdaus Hamid; Nasrudin Andi Mappeware; Efendi Lukas
Molecular and Cellular Biomedical Sciences Vol 5, No 2 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i2.203

Abstract

Background: Leptin resistance which leads to excessive circulating leptin levels is thought to affect ovarian function. This study aimed to study the correlation between serum leptin levels with insulin resistance in patients with polycystic ovary syndrome.Materials and Methods: This cross-sectional study was undertaken in several teaching hospitals in Makassar, Indonesia. We included patients diagnosed with polycystic ovary syndrome (PCOS) aged 18-40 years old. Serum leptin levels were examined in all eligible subjects using the enzyme-linked immunosorbent assay (ELISA) method. The results obtained were further analyzed statistically.Results: Approximately 53 PCOS subjects were included in this study, 25 subjects with insulin resistance and 28 subjects without insulin resistance. After examining serum leptin levels, we found that leptin is directly proportional to insulin resistance (p<0.001). We even found a strong positive correlation between serum leptin levels with homeostatic model assessment for insulin resistance (HOMA-IR) levels (r=0.659; p<0.001). Leptin was found to be independent of HOMA-IR, not influenced by confounding factors such as body mass index (BMI) (p=0.090).Conclusion: There was a significant correlation between serum leptin levels and HOMA-IR values in PCOS patients. This correlation was found to be significant regardless of patient's BMI, therefore is considered to have a direct effect on insulin resistance in PCOS.Keywords: polycystic ovary syndrome, leptin, insulin resistance, HOMA-IR
Choline-deficient High-fat Diet-induced Steatohepatitis in BALB/c Mice Saut Horas Hatoguan Nababan; Seruni Tyas Khairunissa; Erni Erfan; Nafrialdi Nafrialdi; Ening Krisnuhoni; Irsan Hasan; Rino Alvani Gani
Molecular and Cellular Biomedical Sciences Vol 5, No 2 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i2.193

Abstract

Background: Non-alcoholic steatohepatitis (NASH) is an expanding cause of chronic liver disease worldwide, including Indonesia, with higher risk progression to cirrhosis and hepatocellular carcinoma. Preclinical experiments using several mice models have been conducted to clarify its complex pathogenesis. This study was designed to investigate whether BALB/c mice on a choline-deficient high-fat diet can be used as a model for NASH. Materials and Methods: BALB/c male mice were fed choline-deficient L-amino acid-defined high-fat diet (CDAHFD) or a standard diet for six weeks. The body and liver weights, liver histology, and plasma biochemistry were analyzed. The relative expression levels of tumor necrosis factor (TNF)α, transforming growth factor (TGF)β1, collagen-1α1 (COL1α1), glutathione peroxidase 1 (GPx1), and uncoupling protein 2 (UCP2) genes in the livers were analyzed using a two-step real time-polymerase chain reaction. Liver fatty acids composition was analyzed using gas chromatography with flame ionization detector (GC-FID). Results: CDAHFD induced steatohepatitis in BALB/c mice with increased plasma levels of alanine aminotransferase. The liver of CDAHFD-fed BALB/c mice showed upregulated relative expression levels of TNFα, TGFβ1, COL1α1, GPx1, and UCP2 genes. The liver fatty acid analysis showed a significant accumulation of saturated fatty acids (SFAs) and an increased ratio of n-6/n-3 polyunsaturated fatty acids (PUFAs) in the livers of CDAHFD-fed BALB/c mice. Conclusion: This study suggests that CDAHFD can induce steatohepatitis in BALB/c mice and therefore may be used as NASH mice model.Keywords: steatohepatitis, fatty liver, choline-deficient high fat diet, BALB/c 
The Effect of Carnitine Supplementation on Blood Ammonia Level in Epilepsy Children Treated with Valproic Acid: A Randomized Controlled Trial I Gusti Lanang Sidiartha; I Gusti Ngurah Made Suwarba; Dyah Kanya Wati; Ida Bagus Subanada
Molecular and Cellular Biomedical Sciences Vol 5, No 1 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i1.177

Abstract

Background: Long-term use of valproic acid is associated with a high level of blood ammonia related to carnitine deficiency. This study investigates the effect of carnitine supplementation on blood ammonia levels in children with epilepsy who have been treated with valproic acid for more than six months.Materials and Methods: This was a randomized, double-blind, placebo-controlled trial study where children with epilepsy who were treated with valproic acid were randomly allocated to the carnitine supplementation and control group. All children were followed for month, and then measured for blood ammonia level. Blood ammonia levels of both groups were compared using an Independent t-test with a significant of p<0.05.Results: Total of 32 children with epilepsy were enrolled as subjects in this study, with 16 children in carnitine group, and 16 children in control group. Among the subjects, 50% were male and 50% were female, with a mean age of 6.5 years old. The average duration of epilepsy in the carnitine and control group were 41.7 months and 36.9 months, respectively (p=0.419). The duration of valproic acid therapy in the carnitine and control group were 33.1 months and 27.6 months, respectively (p=0.483). The level of blood ammonia in carnitine and control group were 44.6 mg/dL and 81.4 mg/dL, respectively (p=0.007).Conclusion: The level of blood ammonia in a carnitine group was significantly lower than in a control group. It is recommended to give carnitine supplementation in epileptic children treating with long-term valproic acid.Keywords: ammonia, carnitine, epilepsy, seizure, valproic acid
Evaluating The Effect of Humidity on Adhesion Strength of Skin Adhesive Arshad F. Jassem Al-Kaabi
Molecular and Cellular Biomedical Sciences Vol 4, No 3 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i3.148

Abstract

Background: Skin adhesive has been used for attaching certain medical application to the human skin for functional and/or esthetic purposes. Silicone adhesive is the most common type of skin adhesives that are recently used. This study aims to evaluate the possible effect of humidity on the performance of silicone skin adhesive.Materials and Methods: Twenty-four silicone samples were divided into 2 main groups based on relative humidity (RH) exposure, namely 43% RH and 98% RH. Six samples from each group were tested for adhesion strength after 1 hour of adhesion, and the other 6 samples were tested after 2 hours of adhesion by conducting 180 degree peel test. The data were statistically analyzed for significant difference. Results: The results showed that at 43% RH, the adhesion strength was higher than the 98% RH group. The results also showed that at both humidity settings the adhesion strength after the first hours of adhesion was lower than the adhesion strength after the second hour.Conclusion: The silicone skin adhesive performance can be affected by the increase of relative humidity which needs more time of application to skin to reach the best adhesion function.Keywords: adhesions strength, humidity effect on adhesion, silicone adhesive, skin adhesives
Origin, Stemness, Marker and Signaling Pathway of Oral Cancer Stem Cell Dicha Yuliadewi Rahmawati; Hernindya Dwifulqi; Ferry Sandra
Molecular and Cellular Biomedical Sciences Vol 4, No 3 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i3.159

Abstract

Cancer constitutes of complex heterogeneous organ-like structures with a hierarchical cell structure, and only minor phenotypic subpopulations with stem-like properties have a dual capacity to indefinitely self-renew and generate all heterogeneous cell phenotypes consisting of bulk tumor cells. Cancer stem cells (CSC) has similar properties to ordinary stem cells. It is understood that CSC is responsible for the recurrence of metastasis and drug resistance. Thus, control of CSC can provide successful therapy intervention that inhibits cancer growth and aggressive behavior. Conventional cancer therapy is realized to be insufficient for oral cancer therapy. Meanwhile, accurate targeting of OCSC has proved to be a significant challenge due to the commonality of many markers between OCSC and healthy cells. This article discusses the current understanding of oral CSC, with focus on origin, stemness, marker and signalling pathway.Keywords: oral cancer stem cell, CSC, marker, origin, stemness, therapy
The Expression of TGF-b1, p38 MAPK, and ERK-1 Protein in Cleft Affected Tissue of the Lip: An Observational Study Herman Yosef Limpat Wihastyoko; Erdo Puncak Sidarta
Molecular and Cellular Biomedical Sciences Vol 5, No 2 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i2.195

Abstract

Background: Cleft lip is a congenital birth defect caused by many proteins. Transforming growth factor (TGF)-β1, p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated protein kinase (ERK)-1 are proteins which regulate proliferation and apoptosis role during intrauterine period. This study aimed to observe the expression of these proteins in cleft affected tissue of the lip.Materials and Methods: A descriptive study by examining the TGF-β1, p38 MAPK, and ERK-1 immunohistochemical expression of cleft affected tissue of the lip was conducted. Subjects were patients that were participating for the social event held by Plastic Surgery Department, Faculty of Medicine, Univesitas Brawijaya, on December 3-12, 2012 in Nusa Tenggara Timur. Excess lip mucosa (waste tissue) during the operation were stored in 10% formalin then stained by immunohistochemistry for TGF-β1, p38 MAPK, and ERK-1. We counted the average protein expression under the light microscope with 1000x magnification for 20 different fields of view, randomly.Results: Paraffin blocks from 30 subjects were selected. The mean p38 MAPK expression was found to be highest, with the average of 8 per field of view; followed by the mean TGF-β1 expression, with the average of 5 per field of view; and the mean ERK-1 expression was found to be the lowest, with the average of 2 per field of view. Conclusion: Expression of p38 MAPK and TGF-β1 are higher than ERK-1, suggesting that p38 MAPK is in the same signalling pathway as TGF-β1, while ERK-1 is lower, as its role as anti-apoptotic. This is consistent with several previous studies showing that all proteins took part in the development of cleft lip or craniofacial development. Further study needs to be conducted to determine which protein plays the bigger role.Keywords: cleft lip, TGF-β1, p38 MAPK, ERK-1
Vitamin D Status and Cognitive Performance of Post Stroke Patients Rizaldy Pinzon; Vincent Ongko Wijaya; Dessy Paramitha
Molecular and Cellular Biomedical Sciences Vol 5, No 1 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i1.170

Abstract

Background: The prevalence of post-stroke cognitive impairment (PSCI) ranges from 20-80%. Some studies found that vitamin D deficiency was common in stroke patients, yet the relationship with cognitive performance remains unclear. The study aimed to investigate the relationship between levels of vitamin D and cognitive performance in post-stroke patients.Materials and Methods: This was a cross-sectional study with 20 post-ischemic stroke patients. Vitamin D levels were measured using enzyme-linked fluorescent assay (ELFA). The cognitive performance was assessed by computerized mini-mental state examination (MMSE) and clock drawing test (CDT). The relationship between vitamin D levels and cognitive tests were performed using paired T-test.Results: Vitamin D insufficiency (<30 ng/mL) was experienced by all of the study subjects (100%), with the mean±SD of vitamin D level was 13.75±4.06 ng/mL. More than 70% subjects had cognitive impairment. Based on MMSE, patients with cognitive impairment had lower vitamin D levels, compared with those patients with normal cognition (13±4.38 vs. 16±2.44 ng/mL, p<0.001). In CDT examination, patients with cognitive impairment had slightly higher vitamin D levels, compared with those patients with normal cognition (13.93±4.25 vs. 13.33±3.93 ng/mL, p<0.001).Conclusion: The insufficiency of vitamin D on post-ischemic stroke patients appears to generate a bigger chance of PSCI occurrence. Physicians should be aware of vitamin D status of post-stroke patients, especially in the older population.Keywords: vitamin D levels, post-stroke cognitive impairment, risk factors
Intravenous and Oral Paracetamol Have the Same Effect in Reducing Fever in Pediatric Patients Fitri Asymida; Yazid Dimyati; Bidasari Lubis; Aznan Lelo; Muhammad Ali; Ayodhia Pitaloka Pasaribu; Syahril Pasaribu
Molecular and Cellular Biomedical Sciences Vol 4, No 3 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i3.147

Abstract

Background: The antipyretic effect of intravenous versus oral paracetamol is not well known. This study was aimed to compare the antipyretic effect of intravenous and oral paracetamol therapy to reduce fever.Materials and Methods: This was an open-label randomized clinical trial study. The subjects were children who presented to Pediatric Ward and Emergency of Haji Adam Malik Hospital, aged from 2 months to 18 years old, with axillary temperature ≥38.0ºC. Subjects were divided into two groups, group 1 received 15 mg/kg paracetamol intravenous and group 2 received the same dose of paracetamol but given through intravenous. The temperature reduction was analyzed by ANOVA, and the change in temperature was recorded at 0, 15, 30, 60, 120, and 180 minutes after drug administration.Results: In the first group, the mean temperature was decreased (p<0.001) from 15 to 180 minutes after the administration of paracetamol. Nausea was documented as the adverse effect for both oral and intravenous administration groups.Conclusion: The administration of 15 mg/kg paracetamol, either though intravenous or oral, have similar effect in reducing fever in children. Paracetamol therapy though intravenous route can be given if it cannot be given orally.Keywords: antipyretic, pediatrics, fever, intravenous, oral, paracetamol
Correlation between Blood Pressure and Obesity Parameter against Cystatin-C and Adiponectin Levels in Serum of Obese Adolescent Ridwan Ridwan; Ami Febriza; Elmiana Bongga Linggi; Rosdiana Natzir; Nurpudji Astuti Tazlim
Molecular and Cellular Biomedical Sciences Vol 4, No 3 (2020)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v4i3.109

Abstract

Background: Obesity contributes to the increased risk of chronic kidney and systemic inflammation. This condition can occur in obese adolescents. Most researches of cystatin-C (cys-C) and adiponectin have been carried out in adult and children subjects. The purpose of this research is to determine differences in serum cys-C and adiponectin levels between obese and normoweight adolescents and their correlation with blood pressure and obesity parameters.Materials and Methods: Twenty-eight obese adolescents and 22 normoweight adolescents aged 15-18 years old participated in this research. After the measurement of blood pressure and obesity parameters, we examined serum cys-C levels as a biomarker for decreased early stage renal function and adiponectin, an antiinflammatory adipokine. Estimated glomerular filtration rate (eGFR) based on serum cys-C is calculated using the eGFR-Chronic Kidney Disease Epidemiology Collaboration (eGFR-CKD EPI cys-C equation formula). Results: Cys-C levels in obese adolescents were higher than normoweight adolescents. In contrast, serum adiponectin levels in obese adolescents were lower than for normoweight adolescents. Interestingly, eGFR based on serum cys-C showed no difference, although eGFR in obese adolescents was lower than normoweight adolescent. Serum cys-C was positively correlated with systolic blood pressure (SBP), diastolic blood pressure (DBP), body mass index (BMI) and waist circumference (WC). Serum adiponectin was negatively correlated with SBP, DBP, BMI and WC. SBP is a predictor factor for the increase in serum cys-C and BMI is a predictor factor in the decrease in serum adiponectin levels. Conclusion: There is a decline in early stage kidney function and inflammation in obese adolescents as evidenced by increased serum cyst-C levels and decreased serum adiponectin levels. These two biomarkers correlate with blood pressure, BMI and WC and play a role in the pathomechanism of early stage impairment of kidney function and inflammation in obese adolescents.Keywords: adiponectin, body mass index, cystatin-C, early stage renal diseases, inflammation, systolic blood pressure

Page 8 of 18 | Total Record : 174

 6   7   8   9   10 

Filter by Year

2017 2025


Reset
Filter By Issues
All Issue Vol 9, No 2 (2025) Vol 9, No 1 (2025) Vol 8, No 3 (2024) Vol 8, No 2 (2024) Vol 8, No 1 (2024) Vol 7, No 3 (2023) Vol 7, No 2 (2023) Vol 7, No 1 (2023) Vol 6, No 3 (2022) Vol 6, No 2 (2022) Vol 6, No 1 (2022) Vol 5, No 3 (2021) Vol 5, No 2 (2021) Vol 5, No 1 (2021) Vol 4, No 3 (2020) Vol 4, No 2 (2020) Vol 4, No 1 (2020) Vol 3, No 2 (2019) Vol 3, No 1 (2019) Vol 2, No 2 (2018) Vol 2, No 1 (2018) Vol 2, No 1 (2018) Vol 1, No 2 (2017) Vol 1, No 2 (2017) Vol 1, No 1 (2017) Vol 1, No 1 (2017) More Issue