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Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology)
Published by Masyarakat Iktiologi Indonesia
ISSN : 16930339     EISSN : 25798634     DOI : https://doi.org/10.32491
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Aims and Scope Aims: Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) aims to publish original research results on fishes (pisces) in fresh, brackish and sea waters including biology, physiology, and ecology, and their application in the fields of fishing, aquaculture, fisheries management, and conservation. Scope: This journal publishes high-quality articles dedicated to all aspects Aquaculture, Fish biodiversity, Fisheries management, Fish diseases, Fishery biotecnology, Moleculer genetics, Fish health management, Fish biodiversity.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 420 Documents
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Immune responses and growth performance of catfish (Clarias gariepinus Burchell 1822) cultivated in bioflok system with different carbon sources and infected with Aeromonas hydrophila Windu Sukenda; Widanarni Widanarni; Mia Setiawati
Jurnal Iktiologi Indonesia Vol 16 No 3 (2016): October 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i3.30

Abstract

One of the diseases that often attack the catfish is motile aeromonas septicemia (MAS) caused by Aeromonas hydrophila. This study aimed to evaluate the immune responses and growth performance of catfish that cultivated on biofloc systems with different carbon sources and infected by A. hydrophila. This study was conducted over 30 days, consists of five treatments with three replications viz., providing molasses carbon source (A), tapioca flour (B), wheat flour (C), positive control (D) and a negative control (E). The results showed that the immune response such as total erythrocytes, hematocrit, hemoglobin concentration, total leukocyte, phagocytic activity, and respiratory burts activity at molasses (A), tapioca flour (B),and wheat flour (C) treatment showed better results than the control. Carbon sources from molasses, tapioca and wheat were able to increase total bacteria and decrease the growth of A. hydrophila in the waters as well as in catfish organs. Catfish growth performance in biofloc system with tapioca flour carbon source provide daily growth rate which was higher and significantly different (p <0.05) than control. While the biofloc system with molasses, tapioca and wheat carbon source could decrease feed conversion ratio and increase the retention of the protein. Retention of lipid in the biofloc system with molasses carbon source showed the highest results. The addition of molasses, tapioca and wheat as carbon sources into bioflock system could reduce the abundance of A. hydrophila, while immune response and growth performance of catfish increase well. Abstrak Salah satu penyakit yang sering menyerang ikan lele adalah Motil Aeromonad Septicemia yang disebabkan oleh bakteri Aeromonas hydrophila. Penelitian ini bertujuan untuk mengevaluasi respon imun dan kinerja pertumbuhan ikan lele yang dibudidayakan pada sistem bioflok dengan sumber karbon yang berbeda serta diinfeksi oleh A. hydrophila. Peneli- tian dilakukan selama 30 hari, menggunakan rancangan acak lengkap yang terdiri atas lima perlakuan dengan tiga ulangan yaitu penambahan sumber karbon molase (A), tepung tapioka (B), tepung terigu (C), kontrol positif (D), dan kontrol negatif (E). Hasil penelitian menunjukkan bahwa respon imun seperti total eritrosit, hematokrit, kadar hemoglo- bin, jumlah leukosit, aktivitas fagositosis, dan ledakan pernapasan pada perlakuan molase (A), tepung tapioka (B), dan tepung terigu (C) menunjukkan hasil yang lebih baik daripada kontrol. Sumber karbon molase, tapioka, dan terigu mampu meningkatkan total bakteri dan menekan pertumbuhan A. hydrophila di air dan organ ikan lele. Kinerja pertum- buhan ikan lele di sistem bioflok dengan sumber karbon tepung tapioka memberikan laju pertumbuhan harian yang le- bih tinggi dan berbeda nyata (P <0,05) dibandingkan kontrol. Sistem bioflok dengan sumber karbon molase, tapioka, dan terigu dapat menurunkan nisbah konversi pakan dan meningkatkan retensi protein. Retensi lemak dalam sistem bio- flok dengan sumber karbon molase menunjukkan hasil tertinggi. Penambahan sumber karbon molase, tapioka, dan teri- gu dalam sistem bioflok dapat menurunkan kelimpahan A. hydrophila dan meningkatkan respon imun dan kinerja per- tumbuhan ikan lele.
Effect of fasting in waters with different salinity on physiological responses of Asian swamp eel, Monopterus albus Zuiew 1793 Yani Hadiroseyani; Sukenda Sukenda; Enang Harris Surawidjaja; Nur Bambang Priyo Utomo; Ridwan Affandi
Jurnal Iktiologi Indonesia Vol 16 No 3 (2016): October 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i3.31

Abstract

This study aimed to evaluate the physiological responses of Asian swamp eel, Monopterus albus during the fasting period at different salinity levels. Fasting period was three days at four levels of salinity, i.e., 0 g L -1 , 3 g L - 1 , 6 g L - 1 , and 9 g L - 1 . The eels were stocked at a density of 200 kg m - 3 . Physiological parameters studied were blood plasma cortisol which was measured by radioimmunoassay method, blood plasma glucose and liver glycogen which were measured by spectrophotometer, blood plasma sodium which was analyzed by Atomic Absorption Spect rophotometer, and osmolality which was measured using osmometer. The results showed that salinity affected the physiological status of the eels. Fasting the eels could increase the blood plasma cortisol levels, but in at higher salinity levels the hormone was lower. Blood plasma sodium levels of the eels increased in line with higher salinity. Fasting the eels at salinity of 0 and 3 g L - 1 was increasing the glucose levels significantly compared to the eel at a salinity of 6 and 9 g L - 1 . In contrary, the liv er glycogen levels were statistically lower in salinity of 0 and 3 g L - 1 compared to the eels at a salinity of 6 and 9 g L - 1 . Fasting the fish for three days at all salinity treatments did not change the condition factor of the eels. The eels were exhibite d the best physiological performance during the three - days fasting period at a salinity of 9 g L - 1 . Abstrak Penelitian ini bertujuan untuk meng evaluasi respons fisiologis ikan belut Monopterus albus pada pemberokan dengan tingkat salinitas yang berbeda. Pemberokan dilakukan pada ikan belut yang berukuran panjang 26,64 ± 2,94 cm dan b o - bot 12,57±4,99 g , selama tiga hari dalam media air dengan perlakuan empat tingkat salinitas, yaitu 0 g L - 1 , 3 g L -1 , 6 g L - 1 , dan 9 g L - 1 . B iomassa belut adalah 1 kg dalam 5 L air atau pada densitas 200 kg m - 3 . Parameter fisiologis yang diu - kur meliputi kortisol plasma darah dengan metode radioimunoasai, glukosa plasma darah dan glikogen hati dengan spektrofotometer, natrium plasma darah diana lisis dengan Atomic Absorption Spectrophotometer , dan osmolalitas de - ngan menggunakan osmometer. Hasil penelitian menunjukkan bahwa p emberokan dan salinitas mengakibatkan peru - bahan kondisi fisiologis ikan belut. Pemberokan mengakibatkan k adar kortisol meningkat , n amun peningkatan salinitas dapat menekan kadar hormon tersebut. Kadar natrium plasma darah ikan belut mengalami peningkatan sejalan dengan semakin tingginya salinitas. Kadar glukosa belut yang diberok pada salinitas 0 dan 3 g L - 1 secara statistik lebih tinggi dan berbeda sangat nyata di b andingkan dengan belut pada salinitas 6 dan 9 g L - 1 . Hal sebaliknya t erjadi pada kadar gli - kogen hati, yaitu secara statistik lebih rendah pada salinitas 0 dan 3 g L - 1 dibandingkan dengan belut pada salinitas 6 dan 9 g L - 1 . P emberokan selama tiga hari pada semua perlakuan salinitas t idak mengakibatkan perubahan nilai faktor kondisi belut . Ikan belut memberikan respons fisiologis yang paling baik pada pemberokan tiga hari dengan salinitas 9 g L - 1.
Length-weight relationship and condition factor of tonguesole Cynoglossus cynoglossus Hamilton 1822 (Pisces: Cynoglossidae) in Pabean Bay Indramayu, West Java Arinie Gustiarisanie; M. F. Rahardjo; Yunizar Ernawati
Jurnal Iktiologi Indonesia Vol 16 No 3 (2016): October 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i3.32

Abstract

Length - weight relationship and condition factor are two important parameters in fisheries biology. Both of these parameters are equally used for estimating the fish stocks. This study aimed to determine the length - weight relationship and to evaluate the condition factor of tonguesole in Pabean Bay. Fish collection was carried out monthly from January to December. A total of 613 fish consists of 290 males and 323 females. Total length of males and females fish ranged from 46 to 117 mm and 61 to 126 mm with body weight ranged from 0.57 to 9.49 g, 2.36 to 16.72 g, respectively. The length - weight relationship was W = 2x10 - 5 L 2.73 (males and females), W = 5x10 - 5 L 2.56 (males), W = 2x10 - 4 L 2.22 (females) and the condition factor ranged from 0.83 to 1.22 (males), 1.29 to 1.70 (females). These results indicate that the growth pattern of fish was negative allometric and the condition of females fish was better than males. Abstrak Hubungan panjang - bobot dan faktor kondisi merupakan dua parameter penting dalam biologi perikanan. Kedua para - meter ini sama - sama digunakan dalam pendugaan stok ikan. Penelitian ini bertujuan untuk menentukan hubungan panjang - bobot dan mengevaluasi faktor kondisi ikan lidah di Teluk Pabean. Pengambilan ikan contoh dilakukan dalam interval waktu satu kali dalam seb ulan sejak bulan Januari sampai Desember 2015 , menggunakan alat tangkap jaring dan sero. Ikan contoh yang tertangkap sebanyak 613 ekor , terdiri atas 290 ekor ikan jantan dan 323 ekor ikan betina. Panjang total ikan jantan dan betina berkisar 46 - 117 mm dan 61 - 126 mm dengan bobot tubuh ikan jantan dan betina berkisar 0,57 - 9,49 g dan 2,36 - 16,72 g . Hubungan pa njang - bobot ikan lidah adalah W = 2x10 - 5 L 2,73 (jantan dan betina), W = 5x10 - 5 L 2, 56 (jantan), W = 2x10 - 4 L 2, 22 (betina) dan faktor kondisi ikan berkisar 0,83 - 1,22 (jantan) , 1,29 – 1,70 (betina). Hasil ini menunjukkan bahwa pola pertumbuhan ikan bersifat alometrik negatif dan kondisi ikan betina lebih baik daripada ikan jantan.
Enhancement of nonspecific immunity in Aeromonas hydrophilla infected carp, Cyprinus carpio (Linnaeus, 1758) by the administration of peat’s humic acid Diah Wulandari Rousdy; Nastiti Wijayanti
Jurnal Iktiologi Indonesia Vol 16 No 3 (2016): October 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i3.33

Abstract

Peat soils are known to have a high humus content. One of the humus compounds is humic acid. Humic acid has a complex and a different chemical structure for each type of soil. Therefore humic acid has the biological potency, one of them is stimulating the nonspecific immune system. The aim of this study was to determine the effect of humic acid to the phagocytic activity of macrophages and production of neutrophils radical oxidative species (ROS) in carp (Cyprinus carpio L.) that have been infected by the bacteria Aeromonas hydrophilla. In this experiment, we used carp with body length ranged from10-15 cm. Humic acid treatment was given through the feed for 21 days. Treatments include control groups and the humic acid groups (i.e. 1, 3, 5% weight of the feed). The addition of humic acid in carp’s feed was able to increase phagocytic activity. The highest phagocytic index was shown at 3% humic acid treatment and radical oxidative species (ROS) was increased in 1% humic acid treatment. The result of challenge test with A. hydrophilla injected by intraperitoneal route showed the highest survival rate was found in the 1% humic acid treatment. Abstrak Tanah gambut diketahui mempunyai kandungan humus yang tinggi. Salah satu komponen humus dalam gambut adalah asam humat. Senyawa asam humat mempunyai struktur yang kompleks dan berbeda untuk tiap jenis tanah, sehingga memungkinkan asam humat memiliki potensi biologis, salah satunya menstimulus sistem imunitas nonspesifik. Peneli-tian ini bertujuan menentukan pengaruh pemberian asam humat dalam pakan ikan terhadap aktivitas fagosit makrofag dan produksi radikal oksidatif sel neutrofil ikan mas (Cyprinus carpio L.) yang telah diinfeksi bakteri Aeromonas hydrophilla. Ikan yang digunakan berukuran 10-15 cm. Perlakuan pada penelitian ini yaitu dosis asam humat diberikan melalui pakan selama 21 hari. Perlakuan yang diberikan mencakup kelompok kontrol dan perlakuan asam humat (1; 3; 5% bobot pakan). Penambahan asam humat dalam pakan ikan mas cenderung meningkatkan aktivitas fagositik dan pro-duksi radical oxidative species (ROS) meski tidak berbeda dengan kontrol (P>0,05). Peningkatan indeks fagositik ter-tinggi ditunjukkan oleh perlakuan asam humat 3% dan peningkatan ROS diberikan oleh perlakuan asam humat 1%. Hasil uji tantang dengan bakteri A. hydrophilla secara intraperitoneal menunjukkan bahwa asam humat konsentrasi 1% mampu menghasilkan nilai sintasan terbaik.
Some fishing and biological aspets of crocodile shark Pseudocarcharias kamoharai (Matsubara, 1936) in tuna longline in Indian Ocean Prawira A. R. P. Tampubolon; Dian Novianto; Abram Barata
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.34

Abstract

Crocodile shark, Pseudocarcharias kamoharai (Matsubara 1936) is one of the non-target species which is caught by tuna longlines. This shark is the discards fish in tuna longline fisheries and only their liver are utilized. The aims of this research were to determine some aspects relating to the fishing (fishing areas and hook rate) and biological aspect (size distribution and sex ratio) of the crocodile shark. The data were collected directly via observation in tuna longline commercial fishing vessels based in Palabuhanratu, Cilacap and Benoa. The observations were carried out from February 2012 to October 2014 in Indian Ocean. The crocodile sharks were caught at 6o44'42" S to 32o59'49" S latitude and 85o34'34" E to 120o20'35" E longitude. The hook rate of crocodile shark was fluctuated and the average in 20122014 was 0.39. A total of 288 crocodile sharks were caught during the study period. However, only 182 of them could be measured. The size of male crocodile sharks were between 54-112 cm long; while the female fish were 51-117 cm. Sex ratio of crocodile shark was balance. Abstrak Hiu buaya, Pseudocarcharias kamoharai (Matsubara, 1936) merupakan salah satu spesies non target yang turut tertang-kap di rawai tuna. Ikan ini merupakan ikan buangan yang umumnya hanya diambil hatinya saja. Tulisan ini bertujuan untuk menentukan beberapa aspek yang bertalian dengan aspek penangkapan ikan hiu buaya seperti daerah tertangkap dan laju pancing serta sebaran ukuran dan nisbah kelamin. Data dikumpulkan dengan melakukan pengamatan langsung pada kapal penangkap ikan komersial yang menggunakan alat tangkap rawai tuna. Pengamatan dilakukan mulai Februa-ri 2012 hingga Oktober 2014. Kapal komersial ini beroperasi di Samudra Hindia dan berbasis di Palabuhanratu, Cila-cap, serta Benoa. Hiu buaya yang tertangkap menyebar pada posisi 6°44’42”-32°59’49” LS dan 85°34’34”-120°20’35” BT. Laju pancing hiu buaya berfluktuasi dengan nilai rata-rata sebesar 0,39 dalam kurun waktu 2012-2014. Hiu buaya yang tertangkap selama penelitian berjumlah 288 ekor. Namun, hanya 182 ekor yang dapat diukur panjangnya. Hiu buaya jantan memiliki kisaran panjang 54-112 cm; sedangkan ikan betina berukuran antara 51-117 cm. Nisbah kelamin total hiu buaya seimbang.
New localities of the Oryzias woworae species group (Adrianichthyidae) Kazunori Yamahira; Koji Mochida; Shingo Fujimoto; Daniel F. Mokodongan; Javier Montenegro; Takuma Kaito; Asano Ishikawa; Jun Kitano; Taketoshi Sue; Mulis Mulis; Renny K. Hadiaty; Ixchel F. Mandagi; K.W. Alex Maseng
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.35

Abstract

The Oryzias woworae species group, composed of O. asinua, O. wolasi, and O. woworae, is a group of the family Adrianichthyidae endemic to Sulawesi Tenggara (Southeast Sulawesi). Here, we report new localities of each of the three species in this group, which were collected during our field expeditions in 2014-2015. In total, six new localities were discovered throughout Sulawesi Tenggara, including Muna Island, suggesting that they may have wider species ranges than currently recognized. Some of the new localities were independent of the river systems of the known localities, suggesting that each species is genetically structured, and that the unit for conservation should be considered not as each species but as each local population. Abstrak Kelompok spesies Oryzias woworae, family Adrianichthyidae, terdiri atas O. asinua, O. wolasi, dan O. woworae, merupakan spesies endemik di Sulawesi Tenggara. Selama ekspedisi lapangan tahun 2014-2015, kami mencatat setiap spesies dari ketiga spesies dalam grup ini dijumpai di beberapa lokasi baru. Secara keseluruhan, enam lokasi baru telah ditemukan sepanjang daerah Sulawesi Tenggara, termasuk Pulau Muna, yang menunjukkan bahwa grup tersebut kemungkinan memiliki daerah persebaran lebih luas dari yang diketahui saat ini. Sebagian lokasi baru berasal dari sistem aliran sungai yang berbeda dengan lokasi yang telah diketahui selama ini. Hal ini menunjukkan bahwa tiap spesies terstruktur secara genetik, dan bahwa unit konservasi harus dipertimbangkan bukan untuk tiap spesies tetapi untuk tiap populasi lokal.
The phenotypic diversity of three populations of Asian swamp eel Monopterus albus (Zuiew 1793) from West Java and biometrics responses on salinity Ahmad Fahrul Syarif; Dinar Tri Soelistyowati; Ridwan Affandi
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.36

Abstract

This research aimed to evaluate the phenotype diversity of Asian swamp eel from West Java and the biometric responses on salinity for aquaculture development. Fish were collected from three different locations, i.e., Cianjur, Sukabumi, and Karawang with body size of 23.05+0.63 cm and weight of 7.58+1.04 g. Truss morphometric measurement was conducted for 30 samples from each location and biometric response on salty water media for 200 individuals. The experiment was performed in the media with different level of salinity, no substrate and with some shelters. Completely randomized design (CRD) with three levels of salinity (i.e. 0, 6, 12 g L-1) and density 1 kg m-2 was used in this study. Rearing was carried out for 30 days with 100% of media was changed every day and fish were fed with silk worms once a day with at satiation manner. The results showed that phenotypic diversity of Asian swamp eels from Karawang and Cianjur was similar, but differ from Sukabumi population. The biometric responses of Asian swamp eel from Karawang showed the superior performance and the best salinity of media for culture without substrate was 6 g L-1. Abstrak Penelitian ini bertujuan untuk mengevaluasi keragaman fenotipe belut asal Jawa Barat serta respons biometriknya dalam pemeliharaan pada media air bersalinitas untuk pengembangan budi daya. Belut ditangkap dari tiga lokasi yaitu Cianjur, Sukabumi, dan Karawang dengan ukuran tubuh berkisar antara 23,05+0,63 cm dan bobot 7,58+1,04 g. Sebanyak 30 ekor setiap populasi digunakan untuk pengukuran truss morfometrik dan 200 ekor untuk pengujian respons biometrik pada media air bersalinitas. Pemeliharaan dilakukan pada media air bersalinitas tanpa substrat dengan pemberian pelindung. Percobaan menggunakan rancangan acak lengkap dengan perlakuan perbedaan salinitas media pemeliharaan yaitu 0, 6, dan 12 g L-1 dengan padat penebaran 1 kg m-2. Pemeliharaan belut dilakukan selama 30 hari dengan penggantian air 100% setiap hari dengan pemberian pakan berupa cacing sutera Tubificidae sebanyak satu kali per hari secara at satiation. Hasil penelitian menunjukkan bahwa belut asal Karawang dan Cianjur mirip berdasarkan keragaman fenotipenya dibandingkan populasi Sukabumi. Respons biometrik belut asal Karawang paling unggul dan media terbaik untuk pemeliharaan belut tanpa substrat adalah media dengan salinitas 6 g L-1.
Utilization of purslane Portulaca oleracea seed oil as a source of fatty acid essential of common carp Cyprinus carpio Linnaeus 1758 diet Wiwik Hildayanti; Mia Setiawati; Dedi Jusadi
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.37

Abstract

This study attempts to evaluate the use of oils of purslane Portulaca oleracea as a source of an essential fatty in feed of common carp Cyprinus carpio to replace fish oil. The experiment used completely randomized design with 4 treatments and 3 replications, respectively. Feed treatment consists of fish oil, corn oil and coconut oil (P0), purslane seed oil and coconut oil (P1), corn oil and coconut oil (P2), and coconut oil (P3). Fishes with initial body weight 3.11±0.05 g reared in 50x40x35 cm3 aquariums with a density of 10 fish aquarium-1 during 60 days rearing periods. Results showed that diet P1 have different effects (p<0.05) which had the highest daily growth rate (3.5±0.1%), feed efficiency (71.7±4.9%), protein retention (22.1±1.9%) and lipid retention (81.4±4.5%). The survival rate, hepatosomatic index, cholesterol, triglycerides, high density lipoprotein and low density lipoprotein showed no difference (p>0.05). The fatty acid composition of the carps’s body on diet P1 was total of saturated fatty acid 21,4%, monounsaturated fatty acid 36,9%, n-3 fatty acids 10,4%, n-6 fatty acids 16,1% and ratio n-3/n6 is 0,6. Thus, it can be concluded that purslane seed oil can replace fish oil in carp. Abstrak Penelitian bertujuan mengkaji pemanfaatan minyak biji krokot Portulaca oleracea sebagai sumber asam lemak esensial pada pakan ikan mas Cyprinus carpio untuk menggantikan minyak ikan. Percobaan menggunakan rancangan acak leng-kap, terdiri atas empat perlakuan dan tiga ulangan. Pakan perlakuan terdiri atas minyak ikan, minyak jagung, dan minyak kelapa (P0), minyak biji krokot, dan minyak kelapa (P1), minyak jagung dan minyak kelapa (P2), dan minyak ke-lapa (P3). Ikan mas dengan bobot 3,11±0,05 g dipelihara dalam akuarium berukuran 50x40x35 cm3 dengan padat tebar 10 ekor akuarium-1 selama 60 hari. Hasil penelitian menunjukkan perlakuan P1 menghasilkan nilai laju pertumbuhan harian (3,5±0,1%), efisiensi pakan (71,7±4,9%), retensi protein (22,1±1,9%), dan retensi lemak (81,4±4,5%) tertinggi dan memberikan pengaruh yang nyata (p<0,05). Parameter tingkat kelangsungan hidup, indeks hepatosomatik, koles-terol, trigliserida, high density lipoprotein, dan low density lipoprotein tidak menunjukkan adanya perbedaan (p>0,05). Komposisi asam lemak tubuh ikan mas pada perlakuan P1 yaitu total asam lemak jenuh sebesar 21,4%, asam lemak tak jenuh tunggal sebesar 36,9%, n-3 sebesar 10,4%, n-6 sebesar 16,1% dan rasio n-3/n-6 sebesar 0,6. Berdasarkan hasil yang diperoleh disimpulkan bahwa minyak biji krokot dapat menggantikan minyak ikan pada pakan ikan mas
Feminization of Indonesian short-finned eel (Anguilla bicolor bicolor Mc Clelland, 1844) through injection of estradiol and methyl testosterone at the combination with hCG and anti dopamin Abdul Zahri; Agus Oman Sudrajat; Muhammad Zairin Junior
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.38

Abstract

The objective of the experiment was to describe the influence of estradiol (E2) and methyltestosterone (MT) in combination with hCG and anti-dopamine (AD) on the percentage of female and quality of ovary development of Indonesian short-finned eel (Anguilla bicolor bicolor). Eel with 200±15 g of body weight in the yellow phase injected with a estradiol solution of 3mg mL-1 + hCG 2mg mL-1 + AD 10 mg mL-1 (hEA), MT 3 mg mL-1 + hCG 2 mg mL-1 + AD 10 mg mL-1 (hTA), estradiol 3 mg mL-1 + AD 10 mg mL-1 (EA), MT 3 mg mL-1 + AD 10 mg mL-1 (TA), AD 10 mg mL-1 (A) and 9 mg mL-1 NaCl control (F). Injection frequency was six times with a period of two weeks. After six weeks of experiment, EA and hTA combination hormone treatments were effective to increase the female percentage 72% and 66%, respectively. The best ovary development was found in the hTA treatment i.e. attain the late-vitellogenesis with GSI value of 4.80%. Abstrak Penelitian ini bertujuan untuk menjelaskan pengaruh estradiol (E2) dan metil testosteron (MT) yang dikombinasikan dengan hCG dan anti dopamin (AD) terhadap persentase jenis kelamin betina dan kualitas perkembangan ovari sidat (Anguilla bicolor bicolor). Sidat dengan bobot 200±15g pada faseyellow diinjeksi dengan larutan estradiol 3 mg mL-1 + hCG, 2 mg mL-1 + AD 10 mg mL-1 (hEA), MT 3 mg mL-1 + hCG 2 mg mL-1 + AD 10 mg mL-1 (hTA), estradiol 3 mg mL-1 + AD 10 mg mL-1 (EA), MT 3 mg mL-1 + AD 10 mg mL-1 (TA), AD 10 mg mL-1 (A), dan kontrol 9 mg mL-1 NaCl (F). Frekuensi injeksi sebanyak enam kali dengan periode dua minggu sekali. Pemberian hormon kombinasi dengan perlakuan hEA dan hTA ternyata efektif meningkatkan persentase jenis kelamin betina berturut-turut sebesar 72% dan 66% setelah enam minggu. Perkembangan gonad betina terbaik ditemukan pada perlakuan hTA yaitu mencapai fase vitellogenesis akhir dengan nilai GSI 4,80%
Spawning behavior, female reproductive potential and breeding technique optimize of threadfin rainbowfish Iriatherina werneri Muh. Herjayanto; Odang Carman; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 16 No 2 (2016): June 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i2.39

Abstract

Basic information of threadfin rainbowfish especially their reproduction in captivity is little known. Therefore, study on the spawning behaviour and female reproductive potential based on the different treatment of feed (commercial food and natural food) and optimize breeding technique is needed. The study on optimize breeding technique including the effect of different spawning systems (masse or individual), sex ratio (1:1, 1:2 and 1:3), and female size (small, medium and large) to support threadfin rainbowfish culture. The result showed that the threadfin rainbowfish spawn at 13-15 hours after pairing broodfish and the fertilization starts when male spread out and shrink up the fins. The eggs were released faster in the masse than in individual spawing systems and the eggs were released more simultaneously in the morning (94.92%). The threadfin rainbowfish is a partial spawner that spawns every day until 30 days. The potential of eggs and larvae production could be increased up to 4 and 14 times by fed the fish with natural food. Technique for optimize the breeding is using the masse spawning system with sex ratio 1:3 and size range of female is 26.98 to 35.76 mm. Abstrak Informasi reproduksi ikan pelangi Iriatherina werneri pada wadah terkontrol masih sedikit diketahui. Oleh karena itu, diperlukan kajian mengenai tingkah laku memijah, potensi reproduksi ikan betina berdasarkan perbedaan pakan (buatan dan alami), dan optimasi teknik pemijahan. Kajian optimasi pemijahan meliputi pengamatan pengaruh perbedaan sistem pemijahan (massal atau individual), perbedaan rasio kelamin pemijahan jantan : betina (1:1, 1:2, dan 1:3), serta perbedaan ukuran betina (kecil, sedang, dan besar) untuk mendukung kegiatan budi daya. Hasil penelitian menunjukkan bah-wa pemijahan ikan pelangi terjadi pada 13-15 jam sejak pemasangan ikan jantan dan betina yang diawali oleh gerakan ikan jantan mengembangkan dan menguncupkan sirip. Telur yang dikeluarkan pada pemijahan massal berakhir dua jam lebih cepat dibandingkan pemijahan individual dan telur lebih serempak dikeluarkan pagi hari (94,92%). Ikan pelangi merupakan pemijah bertahap yang mampu memijah setiap hari selama 30 hari. Potensi jumlah telur dan larva yang di-hasilkan seekor betina dapat ditingkatkan masing-masing sebanyak empat kali lipat dan 14 kali lipat melalui pemberian pakan alami. Optimasi teknik pemijahan I. werneri dicapai dengan menggunakan sistem massal dengan rasio kelamin 1: 3 dan menggunakan ikan betina berukuran 26,98-35,76 mm.

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