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INDONESIA
Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology)
Published by Masyarakat Iktiologi Indonesia
ISSN : 16930339     EISSN : 25798634     DOI : https://doi.org/10.32491
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Aims and Scope Aims: Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) aims to publish original research results on fishes (pisces) in fresh, brackish and sea waters including biology, physiology, and ecology, and their application in the fields of fishing, aquaculture, fisheries management, and conservation. Scope: This journal publishes high-quality articles dedicated to all aspects Aquaculture, Fish biodiversity, Fisheries management, Fish diseases, Fishery biotecnology, Moleculer genetics, Fish health management, Fish biodiversity.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 420 Documents
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Sex reversal mechanism in Nile tilapia Oreochromis niloticus (Linnaeus, 1758) by manipulation of aromatase gene expression Upmal Deswira; Agus Oman Sudrajat; Dinar Tri Soelistyowati
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.50

Abstract

Sex reversal is a sex conversion technique to be male or female. This research was conducted to evaluate the mechanism of sex reversal in tilapia on the use of aromatase inhibitors (AI), honey and pesticide. The eye spot embryo of Nile tilapia was immersed into those solutions and examined the expression of aromatase gene and sex ratio. The sex reversal was carried out by immersion of eye spot embryo for 24 hours using 20 mg L-1 AI (imidazole), 10 ml L-1 honey (Perhutani, longan flower), and 1 ^L L-1 pesticide (Decis: deltamethrin 25 g L-1). Aromatase gene expression was analyzed on 1st and 8th day after treatment. Larvae were reared until 75 days-old. The level of gene expression of ovarian type aromatase in imidazole, honey, and pesticide treatment on 1st day after treatment were lower than control. While, on 8th day the lowest level of gene expression was on imidazole treatment and the highest was on pesticide treatment. The results indicated that masculinization occurred in imidazole treatment and feminization occurred in pesticide treatment which showed by male percentage that significantly different from the control (p<0.05). The male percentage of control, imidazole, honey, and pesticide were 68.32%, 80.77%, 70.93% and 50.45%, respectively. Honey treatment was not significantly different from control. In conclusion, sex reversal was influenced by modulation of gene expression of ovarian type aromatase, the increasing of gene expression of ovarian type aromatase caused feminization, and otherwise the decreasing of gene expression caused masculinization in early development stage of nile tilapia. Abstrak Alih kelamin merupakan suatu teknik pengalihan kelamin menjadi jantan atau betina. Penelitian ini bertujuan mengeva-luasi mekanisme alih kelamin ikan nila pada penggunaan penghambat aromatase, madu, dan pestisida. Ikan nila fase bintik mata direndam dalam tiga larutan tersebut dan diamati ekspresi gen aromatase dan nisbah kelaminnya. Peren-daman dilakukan selama 24 jam menggunakan penghambat aromatase (imidazole) 20 mg L-1, madu (Perhutani, bunga kelengkeng) 10 ml L-1, dan pestisida (Decis: deltametrin 25 g L"1) 1 ^L L-1. Kemudian dilakukan analisis ekspresi gen aromatase pada hari ke-1 dan ke-8 setelah perlakuan. Selanjutnya larva dipelihara sampai berumur 75 hari. Tingkat ekspresi gen aromatase tipe ovari perlakuan imidazole, madu, dan pestisida pada hari ke-1 setelah perlakuan lebih rendah dibandingkan kontrol. Pada hari ke-8 tingkat ekspresi gen paling rendah pada perlakuan imidazole sedangkan yang paling tinggi pada perlakuan pestisida. Hasil ini menunjukkan bahwa terjadi proses maskulinisasi pada perlakuan imidazole dan feminisasi pada perlakuan pestisida yang diketahui dari persentase jantan berbeda nyata dibandingkan dengan control (p<0,05). Nilai persentase jantan pada perlakuan kontrol, imidazole, madu, dan pestisida secara berurutan yaitu 68,32%, 80,77%, dan 50,45%. Perlakuan madu tidak berbeda nyata dengan kontrol. Disimpulkan bahwa alih kelamin dipengaruhi oleh perubahan ekspresi gen aromatase tipe ovari, peningkatan ekspresi gen aromatase tipe ovari menyebabkan feminisasi, sebaliknya penurunan ekspresi gen menyebabkan maskulinisasi di perkembangan awal larva ikan nila.
Pangasianodon hypophthtalmus (Sauvage 1878) pada sistem bPangasianodon hypophthalmus (Sauvage 1878) juvenile production using biofloc technology with different carbon sources Ita Apriani; Mia Setiawati; Tatag Budiardi; Widanarni Widanarni
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.51

Abstract

Intensive aquaculture system decreases water quality through the increment of metabolic waste products such as organic nitrogen. The biofloc technology is an alternative solution to avoid the impact of high nutrients disposal in aquaculture production system. This study aimed to evaluate the impacts of biofloc technology using different carbon sources on the production performance of juvenile striped catfish, Pangasianodon hypophthalmus. Completely randomized design with 4 treatments (3 replications) was used in this research i.e.: (A) molasses carbon source, (B) tapioca carbon source, (C) wheat carbon source, and (D) without additional carbon. The juveniles length 2.26±0.12 cm, initial average body weight 0.17±0.05 g were reared for 30 days. Twelve glass tanks (60 cm x 30 cm x 40 cm) filled with 36 L freshwater were used as the experimental culture units. The fish were fed three times daily with a commercial feed containing 27% of crude protein. External organic carbon was added daily two hours after feeding at C/N 15 estimated ratio. The observed parameters i.e.: floc profile, the nutritional content of biofloc, water quality, survival rate, final body length, daily growth rate, feed conversion ratio, protein retention, and lipid retention. The best results showed by molasses treatment, the highest fish survival rate (97.41±0.16 %), longest standard length (2.84±0.1 cm) and decreased feed conversion ratio (0.36±0.04). Abstrak Sistem budi daya intensif menurunkan kualitas air melalui peningkatan produk sisa metabolisme seperti nitrogen or-ganik. Penerapan teknologi bioflok adalah solusi alternatif untuk menghindari dampak buruk pembuangan nutrisi tinggi dalam sistem produksi akuakultur. Penelitian ini bertujuan untuk mengevaluasi pengaruh teknologi bioflok yang menggunakan sumber karbon berbeda pada kinerja produksi yuwana ikan patin (Pangasianodon hypophthal-mus). Rancangan penelitian yang digunakan adalah rancangan acak lengkap dengan empat perlakuan (tiga kali ulang-an) yaitu: (A) sumber karbon molase, (B) sumber karbon terigu, (C) sumber karbon tapioka, dan (D) tanpa penam-bahan karbon. Yuwana ikan patin berukuran panjang awal 2,26±0,12 cm ekor-1 dan bobot rata-rata awal 0,17±0,05 g ekor-1 dipelihara selama 30 hari. Dua belas akuarium (60 cm x 30 cm x 40 cm) diisi dengan air 36 L digunakan seba-gai unit percobaan budi daya. Ikan diberi makan tiga kali sehari dengan pakan komersial mengandung protein 27%. Penambahan karbon dilakukan setiap hari 2 jam setelah makan dengan estimasi rasio C/N 15. Parameter pengamatan meliputi: profil flok, kandungan nutrisi tepung flok, kualitas air, kelangsungan hidup, pertumbuhan panjang baku, la-ju pertumbuhan harian, rasio konversi pakan, retensi protein, dan retensi lemak. Perlakuan dengan penambahan sumber karbon molase menunjukkan kelangsungan hidup tertinggi (97,41±0,16 %), pertumbuhan panjang baku (2,84±0,1 cm), dan menurunkan rasio konversi pakan (0,36±0,04).
Cow’s testicles flour as the natural hormone masculinization of Siamese fighting fish, Betta splendens Regan, 1910 Andi Aliah Hidayani; Yushinta Fujaya; Dody Dharmawan Trijuno; Siti Aslamyah
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.52

Abstract

Siamese fighting fish, Betta splendens male is a lovely color ornamental fish with unique shape fins that make it highly demand by the ornamental fish lovers. This study aims to perform sex reversal with masculinization fish production. The study was carried out in two stages i.e.: stage 1 by soaking the 4 days old fish larvae into a solution of cow testicles flour with different doses, stage 2 with different soaking time. Testicular dose tested consists of five levels i.e.: 0 mg L', 20 mg L-1, 40 mg L-1, 60 mg L-1, and 80 mg L-1. Time immersions tested were: 0 hours, 24 hours, 36 hours, 48 hours and 60 hours. The measured parameter was the percentage of male fish produced. The results showed the highest per-centtage of male fish obtained at a dose of 60 mg L-1 and a 24-hour soaking time with a percentage value respectively 88.5% and 87.5%. The study provided information that masculinization technology in a solution of cow testicles applicable for fish larvae. This technology is easy to do so that farmers can use cow's testicles flour for masculinization for their fish production. Abstrak Ikan Cupang, Betta splendens jantan merupakan ikan hias yang memiliki keindahan warna tubuh serta keunikan bentuk sirip sehingga sangat diminati oleh pecinta ikan hias. Penelitian ini bertujuan melakukan pembalikan kelamin dengan menjantankan ikan cupang yang diproduksi. Penelitian dilakukan dalam dua tahap yaitu: tahap pertama dengan meren-dam larva ikan cupang berumur empat hari ke dalam larutan tepung testis sapi dengan dosis berbeda, dan tahap ke dua dengan lama perendaman berbeda. Dosis testis yang diuji terdiri atas lima tingkatan yaitu 0 mg L-1, 20 mg L-1, 40 mgL-1 60 mg L-1, dan 80 mg L-1. Lama perendaman yang diuji adalah: 0 jam, 24 jam, 36 jam, 48 jam, dan 60 jam. Parameter yang diukur adalah persentase ikan jantan yang dihasilkan. Hasil penelitian menunjukkan bahwa persentase ikan berke-lamin jantan tertinggi diperoleh pada dosis 60 mg L-1 dan lama waktu perendaman 24 jam dengan nilai persentasi ber-turut-turut 88,5% dan 87,5%. Hasil penelitian ini memberikan informasi bahwa teknologi penjantanan melalui perendaman dalam larutan testis sapi dapat dilakukan pada larva ikan cupang. Teknologi ini mudah dilakukan sehingga pembudidaya dapat menggunakan tepung testis sapi untuk menjantankan ikan cupang produksinya.
Effect of dietary vitamin E (a-tocopherol) on the reproductive performance of marble goby Oxyeleotris marmorata Bleeker 1852 Denny Wahyudi; Muhammad Zairin Jr; Muhammad Agus Suprayudi
Jurnal Iktiologi Indonesia Vol 16 No 1 (2016): February 2016
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v16i1.53

Abstract

Vitamin E is lipid soluble antioxidant and plays important roles in fish reproduction. This study aimed to examine the effects of feeding diets with different dose of vitamin E on the reproductive performance of female marble goby. Fish were fed with earthworms (Eudrilus eugeniae) having different level of Vitamin E viz. 0 mg (A), 200 mg (B), 400 mg (C), 800 mg (D) and 1600 mg (E) vitamin E kg-1 earthworms.. High-density lipoprotein (HDL), lowdensity lipoprotein (LDL), triglyceride, total cholesterol, estradiol, egg size, gonadosomatic index (GSI), gonadal histology and levels of vitamin E in gonad were determined. The level of total cholesterol in serum was increased on day 30 along with increasing the level of estradiol, HDL, LDL and triglyceride. The egg size 1.20-1.39 mm from diets D has a highest frequency than treatment A, B, C, dan E.The lowest value of GSI was found in the fish from B treatment. This finding was supported by gonadal histology which showed that the ovary development was slower in B treatment than C, D, and E treatments. The highest level of vitamin E in gonad was obtained in fish from D treatment. In conclusion, the result of the experiment indicates that 800 mg vitamin E kg-1 earthworms was the best dose for the reproductive performance of female marble goby. Abstrak Vitamin E merupakan antioksidan yang larut dalam lemak dan berperan dalam reproduksi ikan. Penelitian ini bertujuan untuk menentukan pengaruh dosis yang berbeda pada pakan terhadap kinerja reproduksi ikan betutu betina. Ikan uji di-beri pakan berupa cacing tanah (Eudrilus eugeniae) yang mengandung 0 (A), 200 (B), 400 (C), 800 (D) dan 1600 (E) mg vitamin E kg-1 cacing tanah. Parameter yang dianalisis adalah high density lipoprotein (HDL), low density lipoprotein (LDL), trigliserida, total kolesterol, konsentrasi estradiol, diameter telur, indeks kematangan gonad (IKG), histologi gonad, dan konsentrasi vitamin E gonad. Konsentrasi total kolesterol darah meningkat pada hari ke-30 bersamaan de-ngan meningkatnya konsentrasi estradiol, HDL, LDL, dan trigliserida. Ukuran diameter telur pada perlakuan D (1,201,39 mm) memiliki frekuensi tertinggi dibandingkan dengan perlakuan A, B, C, dan E. IKG pada percobaan B menun-jukkan hasil yang terendah. Hal ini selaras dengan hasil histologi gonad yang menunjukkan bahwa perkembangan gonad perlakuan B lebih lambat dibandingkan dengan perlakuan C, D, dan E. Konsentrasi vitamin E gonad pada perlaku-an D menunjukkan hasil tertingi dibandingkan perlakuan A, B, C dan E. Hasil penelitian menunjukkan bahwa dosis vitamin E sebesar 800 mg kg-1 cacing tanah merupakan dosis terbaik untuk meningkatkan kinerja reproduksi ikan betutu betina.
Yolk absorption efficiency and morphogenesis of the silver arawana Osteoglossum bicirrhosum (Cuvier, 1829) prelarvae at various interactions of temperature and salinity Yuli Wahyu Tri Mulyani; Dedy Duryadi Solihin; Ridwan Affandi
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.54

Abstract

The silver arawana Osteoglossum bicirrhosum (Cuvier, 1829) has been cultured successfully in Indonesia, but still facing obstacles especially handling on the larval life stage. Yolk-sac stage is one of the critical phases in the early development of fish. Yolk-sac larvae of silver arawana use a yolk as food supply. The yolk absorption process influenced by abiotic factors, particularly temperature and salinity. Hence, this study aimed to examine the interaction of temperature and salinity of media in order for optimal yolk-sac absorption and morphogenesis of silver arawana larvae. The research was conducted from November 2013 to January 2014 in the Laboratory of Aquatic Animal Physiology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University. The experimental design was arranged in two factors completely randomized designs, nine treatments with three replications. The first factor was the temperature consisted of 28, 30 and 32°C; the second factor was the salinity consisted of 3, 4 and 5%o. Yolk-sac larvae were reared in aquarium 40 x 30 x 30 cm3 with a density of 4 yolk-sac larvae per aquarium until the yolk was completely absorbed. Larvae were not fed during the experiment. The parameters measured were survival rate, time of yolk absorption, shrinkage rate of yolk, efficiency of yolk utilization, total length, total weight, specific weight growth rate, gradient osmotic oxygen consumption, and morphogenesis of larvae. The results showed that the silver arawana larvae reared at 28°C in combination with all levels of salinity (3, 4 and 5%o) and temperature of 30°C with a salinity of 3%o generate survival rate 100%. Meanwhile, the efficiency of yolk utilization, the fastest growth and morphogenesis were optimally at a temperature of 30°C in interaction with 3%o salinity. Abstrak Arwana silver Osteoglossum bicirrhosum (Cuvier, 1829) telah berhasil dibudidayakan di Indonesia, namun masih mengalami kendala terutama penanganan pada fase pralarva. Pralarva merupakan salah satu stadia yang rentan dalam perkembangan awal hidup ikan. Pralarva arwana silver memiliki kuning telur yang digunakan sebagai cadangan ma-kanan. Penyerapan kuning telur pralarva dipengaruhi oleh faktor abiotik terutama suhu dan salinitas. Penelitian ini ber-tujuan untuk mengkaji interaksi suhu dan salinitas media pemeliharaan agar optimal untuk penyerapan kuning telur dan morfogenesis pralarva arwana silver. Penelitian dilakukan dari bulan November 2013 hingga Januari 2014 di La-boratorium Fisiologi Hewan Air, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor. Penelitian ini meng-gunakan rancangan acak lengkap faktorial dengan sembilan perlakuan dan masing-masing perlakuan memiliki tiga ulangan. Faktor pertama terdiri atas suhu 28, 30 dan 32°C, faktor kedua terdiri atas salinitas 3, 4 dan 5%o. Pralarva di-pelihara di dalam akuarium berukuran 40 x 30 x 30 cm3 dengan padat tebar empat ekor per akuarium, sampai kuning telur terserap di dalam tubuh. Selama pemeliharaan tidak diberikan pakan. Parameter yang diukur yaitu: kelangsungan hidup, waktu penyerapan kuning telur, laju penyusutan kuning telur, efisiensi pemanfaatan kuning telur, panjang total, bobot total, laju pertumbuhan spesifik, gradien osmotik, konsumsi oksigen, dan morfogenesis. Hasil penelitian menun-jukkan bahwa pralarva arwana silver yang dipelihara pada suhu 28°C dengan semua level salinitas (3, 4, dan 5%o) dan suhu 30°C dengan salinitas 3%o memiliki kelangsungan hidup 100%, sedangkan efisiensi pemanfaatan kuning telur, pertumbuhan optimal, dan morfogenesis tercepat pada perlakuan interaksi suhu 30°C dengan salinitas 3%o.
Phenotypes performance of tilapia best, nirwana II, jatimbulan, and sultana using floating net, and pond culture system Ibrahim Satrio Faqih; Dinar Tri Soelistyowati; Odang Carman
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.55

Abstract

Tilapia is one of the introduced fish species in Indonesia and was firstly imported from Taiwan in 1969. Since that time, many efforts have been made to increase its quality through genetic improvement. Some strains of tilapia have been successfully generated such as best, nirwana II, jatimbulan and sultana. In sustainable aquaculture, success of culture production depends on good environmental conditions and water quality. The aim of this study was to evaluate the phenotype performances of four strains of tilapia obtained through selective breeding reared in floating net and ponds. A completely randomized designs with two factors were used in this research, i.e. strains of tilapia and culture system with three replicates. Fifty fish with an average body length of 3-5 cm was reared in ponds and floating net 2 x 2 x 1 m, fed daily in the morning and afternoon during the experiment. Twenty fish from each strain were sampled in every two weeks and fish body length and body weight were measured. Truss morphometric measurement was conducted after 12 weeks of rearing process. The results showed that sultana strain has the highest growth rate, jatimbulan and best strains have the similar survival and feed conversion rates, while the highest biomass was found in the best strain. The nirwana II has the lowest survival rate (18%), but this strain showed the highest feed efficiency. Culture system affects the phe-noltypic variance of truss morphometric, viz. eleven characters in floating net and two characters in pond specimens. Abstrak Nila di Indonesia merupakan ikan introduksi yang didatangkan dari Taiwan pertama kali pada tahun 1969. Dalam peri-ode yang cukup lama, upaya peningkatan kualitas benih ikan dilakukan secara terus menerus melalui perbaikan mutu genetik. Beberapa strain ikan nila yang telah dihasilkan di antaranya nila best, nirwana II, jatimbulan, dan sultana. Da-lam kegiatan perikanan budi daya yang berkelanjutan, faktor lingkungan dan kualitas perairan merupakan pembatas ke-berhasilan usaha budi daya. Tujuan penelitian ini adalah untuk mengevaluasi keragaan fenotipe empat strain ikan nila hasil pemuliaan pada sistem budi daya karamba jaring apung (KJA) dan kolam air tenang. Penelitian ini dirancang menggunakan rancangan acak lengkap pola faktorial yang terdiri atas faktor empat strain ikan nila dan faktor sistem bu-di daya yaitu KJA dan kolam air tenang. Setiap perlakuan diulang tiga kali. Tiap wadah kolam dan KJA ber-ukuran 2 x 2 x 1 m3 dimasukkan benih sebanyak 50 ekor berukuran 3-5 cm, dan diberi pakan pada pagi dan sore selama masa pe-meliharaan. Setiap dua minggu dilakukan sampling masing-masing strain 20 ekor untuk pengamatan panjang dan bo-bot. Setelah 12 minggu pemeliharaan dilakukan pengukuran truss morfometrik. Hasil penelitian menunjukkan bahwa ikan nila strain sultana memiliki laju pertumbuhan tertinggi, jatimbulan memiliki tingkat kelangsungan hidup dan kon-versi pakan yang sama dengan best, biomassa tertinggi dicapai pada nila best, sementara nirwana II memiliki kelang-sungan hidup yang paling rendah (18%) namun memiliki efisiensi pakan yang terbaik. Sistem pemeliharaan memenga-ruhi peningkatan ragam fenotipe truss morfometrik yakni 11 karakter di KJA dan dua karakter di kolam air tenang.
Evaluation of the addition of cinnamon Cinnamomum burmannii leaves extract in diet for growth performance of catfish Pangasianodon hypophthalmus Sauvage, 1878 Febrina Rolin; Mia Setiawati; Dedi Jusadi
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.56

Abstract

This study aimed to evaluate the effects of cinnamon Cinnamomum burmannii leaves extract addition with different doses in the diet for the growth performance of catfish Pangsianodon hypophthalmus. This study consisted of five treatments and three replications. The cinnamon leaves extract mixed into the diet with 5 doses i.e: 0 (control); 0.5; 1; 2; and 4 g kg-1 diet. Catfish (7.43+0.01 g) were reared in 15 aquariums (160 litres volume) with a density of 30 fishes in each aquarium for 60 days. Fishes were fed at satiation three times daily at 08.00, 12.00, 16.00 WIB. The addition of cinnamon leaves extracts in the amount of 1 g kg-1 diet showed the optimal result because this dose can improve protein retention and feed efficiency in a value at 24.6% and 23.4% with the same growth rate with the treatment without additional cinnamon leaves extract (control). Abstrak Penelitian ini bertujuan untuk mengevaluasi pengaruh penambahan ekstrak daun kayu manis Cinnamomum burmannii dengan dosis berbeda pada pakan terhadap kinerja pertumbuhan ikan patin Pangasianodon hypophthalmus. Penelitian ini terdiri atas lima perlakuan dan tiga ulangan. Ekstrak daun kayu manis dicampurkan ke dalam pakan dengan lima dosis yaitu: 0 (kontrol); 0,5; 1; 2; dan 4 g kg-1 pakan. Ikan patin (7,43+0,01 g) dipelihara dalam 15 akuarium (volume 160 liter) dengan kepadatan 30 ekor/akurium selama 60 hari. Ikan diberi pakan secara at satiation sebanyak tiga kali sehari pada pukul 08.00, 12.00 dan 16.00. Hasil penelitian menunjukkan bahwa penambahan ekstrak daun kayu manis sebanyak 1 g kg-1 pakan memberikan hasil yang optimal karena dapat meningkatkan retensi protein dan efisiensi pakan sebesar 24,6% dan 23,4% dengan laju pertumbuhan yang sama dengan perlakuan tanpa penambahan ekstrak daun kayu manis (kontrol).
Hormonally induced gonadal maturation in eels, Anguilla bicolor bicolor McClelland 1984 with the use of Pregnant Mare Serum Gonadotropin, anti dopamin, and recombinant Growth Hormone Hadra Fi Ahlina; Agus Oman Sudrajat; Tatag Budiardi; Ridwan Affandi
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.57

Abstract

The aim of this study was to evaluate the effect of Pregnant mare serum gonadotropin (PMSG), Anti-Dopamine (AD) and Recombinant growth hormone (RGH) through the injection technique on gonadal development of eel (Anguilla bicolor bicolor). The experimental was arranged in completely randomized design with five treatments of hormone combination, namely P10A combination hormone (10 IU PmsG + 0.1 mgL-1 AD), P10B (10 IU PMSG + 0.1 mgL-1 AD + 10 ug RGH), P20A (20 IU PMSG + 0.1 mgL-1 AD), P20B (20 IU PMSG + 0.1 mgL-1 AD + 10 ug RGH), and PK (negative control). In each treatment, a total of 20 fish individuals used as replicates and the hormonal induction was conducted every week for eight weeks of the rearing period. Specific growth rate (SGR), hepatosomatic index (HSI), go-nadosomatic index (GSI) and an index of the eye (IM) were observed. The results showed that the P20A treatment increased spermatogenesis and the value of GSI (2.291±0.278%) at 4-6 weeks after injection. This result was higher than P20B (2.134±0.265%), P10B (2.065±0.201%), P10A (2.037±0.105%), and PK (1.937±0.050%). The value of HSI on the P20a treatment (1.188±0.091 %) was higher than other treatments. The highest value of SGR (0.514±0.062%) was found in the P20B, whereas the lowest value (0.052±0.027%) was found in the PK. Thus, the combination of pMsG, AD and RGH hormones can stimulate the development of testicular of eel with body weight 140 to150 g, and stimulate the growth of 0.514 % during the six-weeks rearing period. Abstrak Penelitian ini bertujuan mengevaluasi penggunaan hormon Pregnant Mare Serum Gonadotropin (PMSG), Anti Dopamin (AD) dan Recombinant Growth Hormone (rGH) melalui teknik penyuntikan terhadap pematangan gonad ikan sidat (Anguilla bicolor bicolor). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan lima perlakuan kombinasi hormon yaitu 10 IU PMSG + 0,1 mgL-1 AD (P10A), 10 IU PMSG + 0,1 mgL-1 AD + 10 ^g rGH (P10B), 20 IU PMSG + 0,1 mgL-1 AD (P20A), 20 IU PMSG + 0,1 mgL-1 AD + 10 ^g rGH (P20B), dan PK (kontrol). Pada setiap perlakuan, sebanyak 20 ekor ikan digunakan sebagai ulangan individu dan sampling dilakukan setiap minggu selama delapan minggu masa pemeliharaan. Parameter yang diamati meliputi nilai laju pertumbuhan spesifik (LPS), indeks he-patosomatik (IHS), indeks gonadosomatik (IGS) dan indeks mata (IM). Hasil penelitian menunjukkan bahwa perlakuan P20A menyebabkan perkembangan spermatogenesis dan meningkatkan nilai IGS tertinggi (2,291±0,278%) pada minggu ke empat hingga ke enam setelah penyuntikan dibandingkan perlakuan P20B (2,134±0,265%), P10B (2,065± 0,201%), P10A (2,037±0,105%) dan PK (1,937±0,050%). Nilai IHS pada perlakuan P20A (1,188±0,091%) lebih tinggi dibandingkan dengan perlakuan lainnya. Nilai LPS tertinggi ditemukan pada perlakuan P20B (0,514±0,062 %) dan te-rendah pada perlakuan Kontrol (0,052±0,027%). Nilai IM juga meningkat pada perlakuan P20B (10,599±2,372) seiring dengan bertambahnya bobot tubuh dan terendah pada perlakuan PK (7,189±0,217). Kombinasi hormon PMSG, AD dan rGH dapat merangsang perkembangan testis ikan sidat ukuran 140-150 g serta memacu pertumbuhan 0,514 %.
Effect of cinnamon, Cinnamomun burmanii, leaves extract for non specific immune response in striped catfish Pangasianodon hypophthalmus (Sauvage, 1878) infected by Aeromonas hydrophila nFN Safratilofa; Dinamella Wahjuningrum; Dedi Jusadi; Mia Setiawati
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.58

Abstract

Aeromonas hydrophila causes Motile Aeromonads Septicemia disease in catfish. Phytopharmaca can be performed as an alternative to increasing the nonspecific immune response of catfish. One of the potentially phytopharmaca is cinnamon, Cinnamomum burmanii. This study was conducted to evaluate the potential of cinnamon leaves to increase the nonspecific immune responses of infected catfish by A. hydrophila. Cinna-mon leaf extract was given through fish diet at doses 0.5% and 1% for fourteen days. The challenge test was performed on the day 15. The method used in this study was a completely randomized design with six treatments and three replications. The six treatments were the preventive treatment of 0.5% (A) and 1% (B), the controlling treatment of 0.5% (C) and 1% (D), negative control (K -) and positive control (K +). The results showed that the controlling treatment of 0.5% (C) was the best treatment with the phagocytic activi-ty of blood cells of 77.59±2.50% and respiratory burst activity of 0.072 ± 0.004 after challenge test. More-over, the survival rate of fish was 100% in the C treatment, whereas in the positive control (K+) only 63.33%. Abstrak Bakteri Aeromonas hydrophila dapat menyebabkan penyakit Motile Aeromonads Septicaemia pada ikan patin. Penggunaan fitofarmaka dilakukan sebagai alternatif peningkatan respon imun non spesifik ikan patin. Salah satu bahan fitofarmaka yang dapat dimanfaatkan adalah tumbuhan kayu manis Cinnamomum burmanii. Penelitian ini bertujuan untuk mengevaluasi potensi daun kayu manis dalam meningkatkan respon imun non spesifik ikan patin yang diinfeksi A. hydrophila. Daun kayu manis diberikan dalam bentuk ekstrak melalui pakan dengan dosis 0,5% dan 1% selama 14 hari. Uji tantang dilakukan pada hari ke-15. Metode dalam penelitian ini menggunakan rancangan acak lengkap (RAL) dengan enam perlakuan dan masing-masing tiga ulangan. Perlakuan pencegahan 0,5% (A) dan 1% (B), perlakuan pengendalian 0,5% (C) dan 1% (D), serta kontrol negatif (K-) dan positif (K+). Hasil penelitian menunjukkan bahwa perlakuan C merupakan hasil terbaik dalam meningkatkan respon imun non spesifik pada ikan patin pascauji tantang. Hal tersebut terlihat dari peningkatan aktifitas fagositik 77,59±2,50% dan aktifitas ledakan respiratori 0,072±0,004 ikan patin dan juga pada dosis ini tingkat kelangsungan hidup ikan mencapai 100% sementara pada kontrol positif tingkat kelangsungan hidup ikan hanya 63,33%.
Cytochrome C oxydase 1 gene sequences long-barbel sheatfish, Kryptopterus limpok (Bleeker, 1852) from Kampar River and Indragiri River of Riau Province Roza Elvyra; Dedy Duryadi Solihin
Jurnal Iktiologi Indonesia Vol 15 No 3 (2015): October 2015
Publisher : Masyarakat Iktiologi Indonesia (Indonesian Ichthyological Society)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32491/jii.v15i3.59

Abstract

This study aimed to analyze the sequence of mitochondrial DNA cytochrome c oxydase 1 (Cox-1) gene of the long barbel sheatfish, Kryptopterus limpok. The Cox-1 gene of Kryptopterus limpok from Kampar and Indragiri Rivers, Riau Province was amplified using PCR technique. The length of the Cox-1 gene sequence was 655 bp containing nine nucleotide sites substituted. Four base substitutions were transition and five base was transversion. Nucleotide frequencies of guanine was a few (G:17.2%) and approximately equal in frequencies of cytosine, thymine, adenine (C: 29.5%, T: 27.5%, A: 25.8%), and G+C content (46.7%). The genetic distance of mtDNA Cox-1 gene sequences between Kryptopterus limpok from Kampar and Indragiri river was 0.01. Phylogenetic tree showed that Kryptopterus limpok from Kampar and Indragiri Rivers are in one cluster with 100% bootstrap value. Abstrak Penelitian ini bertujuan untuk menganalisis runutan gen cytochrome c oxydase 1 (Cox-1) DNA mitokondria ikan lais janggut, Kryptopterus limpok. Gen Cox-1 Kryptopterus limpok dari Sungai Kampar dan Indragiri, Provinsi Riau telah diamplifikasi menggunakan teknik PCR. Runutan gen Cox-1 yang diperoleh sepanjang 655 bp, dengan sembilan situs substitusi nukleotida, meliputi empat substitusi transisi dan lima substitusi transversi basa. Frekuensi nukleotida yang sedikit ditemukan pada guanin (G:17,2%), sedangkan frekuensi cytosin, thymin, adenin kurang lebih sepadan (C: 29,5%, T: 27,5%, A: 25,8%), dan kandungan G+C (46,7%). Jarak genetik runutan gen Cox-1 antara Kryptopterus limpok dari Sungai Kampar dan Sungai Indragiri adalah 0,01. Pohon filogeni memperlihatkan bahwa Kryptopterus limpok dari Sungai Kampar dengan Sungai Indragiri membentuk satu kelompok dengan nilai bootstrap 100%.

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