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INDONESIA
JFIOnline
Published by Ikatan Apoteker Indonesia
ISSN : 14121107     EISSN : 2355696X     DOI : -
Core Subject : Health,
Health Professions Medicine & Pharmacology
Jurnal Farmasi Indonesia yang diterbitkan oleh Pengurus Pusat Ikatan Apoteker Indonesia. Isi website memuat seluruh jurnal yang telah diterbitkan mencakup semua aspek dalam ilmu pengetahuan dan teknologi kefarmasian antara lain farmakologi, farmakognosi, fitokimia,farmasetika, kimia farmasi, biologi molekuler, bioteknologi, farmasi klinik,farmasi komunitas, farmasi pendidikan, dan lain-lain.
Arjuna Subject : -
Articles 443 Documents
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ISOLASI SENYAWA SITOTOKSIK TERHADAP SEL KANKER PAYUDARA DARI KULIT BATANG Garcinia griffithii T. Anders Wahyuni, Fatma Sri; Lusianti, Mittha; Almahdy, .; Dachriyanus, .
Jurnal Farmasi Indonesia Vol 4, No 4 (2009)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v4i4.27

Abstract

A cytotoxic compound was isolated from n-hexane the stem bark of Garcinia griffithii T. Anders. (Guttiferae) as colourless needles from which melted at 242-243oC. Based on spectroscopies data of this compound was identified as isoxanthocymol. Cytotoxic assay using Microculture Tetrazolium (MTT) assay showed that isoxanthocymol was active against breast cancer cell line MCF-7, with IC50 value 35,46 μg/ml.   ABSTRAK Telah diisolasi senyawa sitotoksik dari kulit batang Garcinia griffithii T. Anders. (Guttiferae). Senyawa diisolasi dari fraksi n-heksana berupa amorf tidak berwarna dengan jarak leleh 242-2430C. Dari data spektroskopi massa, 1H-RMI, 13C-RMI, diketahui bahwa senyawa tersebut adalah isoxanthocymol. Pengujian aktifitas sitotoksik isoxanthocymol dilakukan dengan metode Microculture Tetrazolium (MTT) terhadap sel kanker payudara MCF-7, dan memperlihatkan aktifitas dengan nilai IC50 sebesar 35,46 μg/ml.
KERASIONALAN PENGGUNAAN ASIKLOVIR PADA SALAH SATU POLIKLINIK KULIT DAN KELAMIN DI SUMATERA SELATAN Suardi, Muslim; Erjon, .; Khodijah, .
Jurnal Farmasi Indonesia Vol 3, No 4 (2007)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i4.91

Abstract

The aim of this study was to observe the rationality of acyclovir use and to provide information in order to improve quality of medical services at a hospital in Sumatera Selatan. A descriptive survey studies was conducted on 77 patients Herpes simplex Virus (HSV), and Varicella zoster Virus (VZV) infections at Ambulatory Clinic of Dermato-Venereology. Data were collected from status cards in medical record installation using systematic sampling method. Data were analyzed quantitatively and qualitatively as well. Results showed that the score of rationalities in drug therapy regarding the appropriateness of indication, drug, and patients, were 100, 89.6, and 100% respectively. While rationalities regarding the appropriateness of: dose, route, time, duration, frequency, and interval of administrations were 49.4, 88.3, 0, 0, 72.7, and 72.7% respectively. As a conclusion, the usage of acyclovir at Ambulatory Clinic of Dermato-Venereology at a hospital in Sumatera Selatan Province hospital were irrational. ABSTRAK Penelitian ini bertujuan untuk mengetahui kerasionalan penggunaan ACV dan diharapkan sebagai bahan masukan untuk meningkatkan mutu pelayanan kesehatan di salah satu Rumah Sakit di Provinsi Sumatera Selatan. Satu rancangan penelitian survei deskriptif dilakukan terhadap 77 pasien infeksi Virus Herpes simpleks (VHS), dan Virus Varisela zoster (VVZ) yang berobat di Poliklinik Kulit dan Kelamin. Data diperoleh dari kartu status pasien pada instalasi rekam medik selama tahun 2005. Selanjutnya data ditabulasikan dalam tabel, dianalisis secara kuantitatif maupun kualitiatif dan dipaparkan dalam bentuk grafik. Hasil penelitian menunjukkan bahwa penilaian kerasionalan yang mencakup tepat indikasi, tepat obat, dan tepat penderita, masing-masing adalah, 100; 89,6; dan 100%. Sedangkan penilaian kerasionalan yang mencakup tepat dosis, tepat rute, tepat saat, tepat lama, tepat frekuensi, dan tepat interval pemberian masing-masing adalah: 49,4 ; 88,3; 0; 0; 72,7; dan 72,7%. Sebagai kesimpulan, penggunaan ACV pada Poliklinik Kulit dan Kelamin salah satu Rumah Sakit di Provinsi Sumatera Selatan adalah tidak rasional.
Kepatuhan Penggunaan Obat pada Pasien Hipertensi di Puskesmas Saepudin, .; Padmasari, Siwi; Hidayanti, Puri; Ningsih, Endang S
Jurnal Farmasi Indonesia Vol 6, No 4 (2013)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.338 KB) | DOI: 10.35617/jfi.v6i4.148

Abstract

In addition to antihypertensive regimen, medication adherence plays an important role in therapeutic management of hyperensive patients. This research is aimerd to measure adherence to antihypertensive therapy in hypertensive patients at primary health center, and to investigate patient's characteristic associated with such adherence. Hypertensive patients attending two primary health centers in Sleman Yogyakarta during January-March 2011 were recruited as respondents in this research. Adherence to natihypertensive therapy was measured using MMAS (Morisky Medication Adherence Scale) containing 8 questions, and a respondent with MMAS score � 6 was categorized as adherent one. Statistical analysis using Chi-square has been done to investigate respondent characteristic associated with adherence. Out of 215 recruited respondents, 62.3% were adherent to antihypertensive therapy. Respondent characteristic associated with adherence are educational background level, monthly income, presence of other chronic disease, and antihypertensive regimen.Keywords : hypertension, medication sdherence, MMAS, primary health center
Gel Kombinasi Ekstrak Daun Sirsak dan Daun Jambu Biji Sebagai Obat Anti Jerawat Yulianti, Rika; Abdassah, Marline; Abdulah, Rizky; Surachman, Emma
Jurnal Farmasi Indonesia Vol 7, No 3 (2015)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.433 KB) | DOI: 10.35617/jfi.v7i3.308

Abstract

This study aims to determine the formulation and testing of anti-acne combination gel activity of ethanol extract of leaves of soursop (Annona muricata L.) and leaves of guava (Psidium guajava L ) in in - vitro and in - vivo. Soursop leaf is traditionally used to treat acne . It is known that asetogenin soursop leaves contains compounds acetogenin, tannins, phytosterols, calcium oxalate, murisin alkaloids, ô°la- vonoids and steroid. Research on anti- acne activity of guava leaf extract has been carried out by Qaâ??dan et al in 2005. Samples were obtained from a combination of extracts of leaves of soursop (Annona muricata L.) and leaves of guava (Psidium guajava L ) using the method of maceration with 96 % ethanol. The preparation is formulated in the form of a gel and tested the minimum inhibitory concentration and antibacterial activity against Propionibacterium acne using Cindala ® gel for comparison. In-vivo studies conducted using rats (Rattus novergicus) galus Wistar as test animals by administration of Propionibacterium acne bacteria colonies suspense as inducers.The results showed that the combination gel of soursop leaf extract and guava leaf extract has anti bacterial activity against Propionibacterium acne with carbomer gel formulation using as a base. In vivo testing results show that the gel has anti acne activity and statistically signiô°icantly different to the negative control.
Validasi Metode Kromatografi Cair Kinerja Tinggi Untuk Analisis Rhodamin B Dalam Saos Tomat Yantih, Novi; Aziz, Zuhelmi; Aprillia, Heidy
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.278 KB) | DOI: 10.35617/jfi.v8i2.520

Abstract

Rhodamine B is a synthetic red dye which is commonly used as textile dyes and banned its use in food and beverages. The red dye is harmful to health and it can cause poisoning symptoms if it is swallowed, and it can damage the liver when it is taken long term. Although it has been banned because of its toxicity, but there is abuse of rhodamine B as a food coloring, such as in tomato sauce product. The purpose of this study is to validate the high performance liquid chromatography (HPLC) for analysis of rhodamine B in a tomato sauce. The analytical method used is HPLC with reverse phase system. The analytical method has met the requirements of method validation according to the International Conference of Harmonization with the detection and quantitation limits, 1 ppm and 3 ppm respectively. Based on the results of the qualitative analysis of rhodamine B in tomato sauce samples, one of the three samples of tomato sauce that is not listed in NA-DFC (The National Agency of Drug and Food Control) showed positive result, it was shown with the same retention time between samples and standards (5.5 minutes). Average level of rhodamine B in the sample was 25 μg/g of tomato sauce.
KAJIAN MEDICATION ERROR PADA KASUS STROKE DI RS X SURAKARTA TAHUN 2004 Mutmainah, Nurul
Jurnal Farmasi Indonesia Vol 4, No 1 (2008)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v4i1.8

Abstract

This work aimed to study the medication error occurred on stroke management at X Hospital in Surakarta during January-October 2004. Subject of the study were in-patient stayed at the hospital during January- July 2004 (retrospective study) and at September-October 2004 (prospective study). Result of the study showed that medication error had been occurred on 49 out 52 patient (94.23%) stayed at the hospital during January-July 2004 and on 16 out 18 (88.89%) patient stayed during September-October 2004. It was revealed that the types of medication error found in retrospective study were prescribing error (26.09%), wrong administration technique (68.12%) and monitoring error (5,9%). Non-rationale antibiotic use was 92.31% and unproper management of hyperglycemic in stroke patient was 45.45%. From prospective study it was revealed that the types of medication error found were prescribing error (25%) and wrong administration technique (75%). Non-rationale antibiotic use was 78.6%, unproper management of hyperglycemic in stroke patient was 12,5%, and medication side effect was 1,92%. ABSTRAK Penelitian ini bertujuan untuk mengkaji kejadian medication error pada terapi stroke di RS X Surakarta selama Januari-Oktober 2004. Subjek penelitian adalah pasien stroke yang menjalani rawat inap di RS X Surakarta periode Januari- Juli 2004 (studi retrospektif) dan periode September-Oktober 2004 (studi prospektif). Dari data yang diperoleh dilakukan kajian penggunaan obat dan outcome pasien yang memberi petunjuk telah terjadinya medication error. Pada studi retrospektif ditemukan adanya kejadian medication error dengan tipe prescribing error (26,09%), wrong administration technique (68,12%) dan monitoring error (5,9%). Penggunaan antibiotik yang kurang tepat ditemukan sebesar 92,31% dan penanganan hiperglikemi pada stroke yang kurang tepat sebesar 45,45%. Dari studi prospektif ditemukan kejadian medication error dengan tipe prescribing error (25%) dan wrong administration technique (75%). Penggunaan antibiotik yang kurang tepat sebesar 78,6%, penanganan hiperglikemi pada stroke yang kurang tepat sebesar 12,5%, dan kejadian efek samping obat sebesar 1,92%. Dari penelitian ini dapat disimpulkan bahwa dari 49 dari 52 (94,23%) pasien stroke pada bulan Januari-Juli 2004 dan 16 dari 18  (88,89%) pasien stroke pada bulan September-Oktober 2004 mengalami kejadian medication error.
FORMULATION OF ANTI-PLAQUE TOOTHPASTE FROM STANDARDIZED GAMBIR EXTRACT AND ITS ANTIMICROBIAL ACTIVITY Lucida, Henny; Rustini, .; Saufitri, Dian; Dachriyanus, .
Jurnal Farmasi Indonesia Vol 5, No 2 (2010)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v5i2.40

Abstract

Telah dilakukan formulasi pasta gigi ekstrak gambir terstandarisasi serta uji daya antimikrobanya terhadap pertumbuhan bakteri penyebab plak Streptococcus mutans. Uji pendahuluan menunjukkan bahwa ekstrak gambir terstandarisasi pada kadar 7% memiliki diameter hambat optimal terhadap pertumbuhan mikroba uji. Berdasarkan ini dibuat 3 formula pasta gigi dengan kadar ekstrak gambir terstandarisasi sebesar 7% dengan kosentrasi kalsium karbonat (abrasif) yang bervariasi, berturut-turut 37, 42 dan 47%. Semua formula diuji sifat-sifat farmasetik dan aktivitas antimikrobanya, sebagai pembanding digunakan pasta gigi â??Fâ?. Hasil evaluasi sifat farmasetika sediaan menunjukkan semua formula memenuhi persyaratan pastagigi yang baik.  Formula 3 (F3) memberikan diameter hambat paling baik (19,25 + 0,50 mm), sedangkan pembanding â??Fâ? memberikan diameter hambat (20,0 + 0,00 mm).  Terdapat perbedaan bermakna diameter hambat antar perlakuan (basis versus pasta gigi ekstrak gambir) pada taraf kepercayaan  0,05.   ABSTRACT A toothpaste containing standardized gambir extract had been formulated and an antimicrobial assay of the toothpaste against Streptococcus mutans had been done. Preliminary study showed that the concentration of gambir extract of 7 % obtained an optimal range of inhibition of S. mutans growth. Three formulas were then prepared, which contained 7 % extract and various concentrations of calcium carbonate (37, 42 and 47 % respectively). The formulas were subjected to evaluation procedures including the pharmaceutical properties and the antimicrobial activity in comparison to commercial formula â??Fâ?. Results indicated that all formulas had good pharmaceutical properties. F3 showed the best inhibition against the growth of S mutans (diameter of inhibition =  19,25 + 0,50 mm) while that of the commercial toothpaste â??Fâ? was (20,0 + 0,00 mm) , which was significantly different from that of the toothpaste  base at probability 0.05.
UJI SITOTOKSISITAS IN VITRO SEDIAAN JADI BRM (BIOLOGICAL RESPONSE MODIFIER) TERHADAP SEL KANKER SERVIKS Harahap, Yahdiana; Reksodiputro, Harryanto; Heffen, Wan Lelly; Krismartina, Mirna
Jurnal Farmasi Indonesia Vol 3, No 2 (2006)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v3i2.72

Abstract

Cancer was the third big death-caused disease in the developed countries. To overcome the disease, cancer patient can use combinaton of conventional and alternative therapy, such as using herbal   medicine. Nowadays there are many anti cancer herbal medicines in the market, one of which was BRM (Biological Response Modifier), that contain tansinon, polisaccharide, Lycium barbarum, Codonopsis pilosulae Radix extract, and marine algae extract. To investigate the cytotoxic effect of BRM, we  conducted cytotoxicity test in vitro using cancer cell culture. The test examined the activity of ethanol and aquoeus extract of BRM  on Ca Ski cells (cervix cancer cells), using Neutral Red Intake Cytotoxicity Test Method.  Results of the experiment revealed the LC50 of ethanol and aquoeus extract were 274.16 and 55.21mg/ml consecutively, after 24 hour incubation and 118.58 and 35.17 mg/ml after 48 hours incubation. It was concluded that ethanol and aquoeus extract of BRM had low cytotoxicity due to LC50 value greater than 20 mg/ml. ABSTRAK Kanker merupakan penyebab kematian ketiga terbesar di negara berkembang. Untuk mengatasi penyakit tersebut, penderita kanker dapat mengkombinasikan terapi konvensional dengan terapi alternatif, yaitu dengan menggunakan sediaan herbal. Saat ini banyak sekali sediaan herbal antikanker yang beredar di pasaran, diantaranya sediaan jadi BRM, yang mengandung tansinon, polisakarida, Lycium barbarum, ekstrak Codonopsis pilosulae Radix, dan ekstrak marinealgae. Untuk mengetahui efek sitotoksik sediaan tersebut maka dilakukan uji sitotoksisitas secara in vitro menggunakan biakan sel kanker. Pengujian sitotoksisitas ekstrak etanol dan air sediaan jadi BRM dilakukan terhadap sel Ca Ski (sel kanker serviks) meggunakan metode Uji Sitotoksisitas Ambilan Merah Netral. Dari penelitian ini diperoleh nilai LC50 ekstrak etanol dan air setelah inkubasi 24 jam masing-masing sebesar 274, 16 dan 55,21mg/ml, sedangkan setelah inkubasi 48 jam sebesar 118,58 dan 35,17 mg/ml. Hasil tersebut menunjukkan bahwa ekstrak etanol dan air sediaan jadi BRM mempunyai efek sitotoksik rendah karena memiliki nilai LC50 lebih besar dari 20 mg/ml.
Identifikasi Senyawa Antikanker dari Fraksi Kloroform Kulit Batang Bruguiera gymnorhiza dan Aktivitas Sitotoksiknya Warsinah, .; Sismindari, .; Susidarti, Ratna Asmah
Jurnal Farmasi Indonesia Vol 6, No 2 (2012)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (979.176 KB) | DOI: 10.35617/jfi.v6i2.129

Abstract

Bruguiera gmnorhiza is one of mangrove plant that has not been widely studied as potential anticancer compounds. Previous research noted that the methanol extract of the bark of B.gymnorhiza are cytotoxic towards HeLa cells. The purpose of this study was to determine the compounds in the chloroform fraction and cytotoxic activity in HeLa cells. Stem bark of B. gumnorhiza was extracted with methanol, further fractionation by partitioning method, using of the solvent h-hexane and chloroform. Fraction of chloroform was evaporated, the tested cytotoxic activity against HeLa cells by MTT method and apoptotic induction by double staining method. Faction levels are used for 500, 250, 125, 62.5 and 31.25 mg/mL with 24-hour incubation time. Subsequent fractions were identified by GC-MS method. The result showed that the chloroform fraction contains 21 compounds, 6 in the form of fatty acids and terpenoids that have anticancer cytotoxic activity (IC50) of 134 ug/mL with apoptotic mekanism.Keywords: Apoptosis, Bruguiera gymnorhiza, cytotoxic, Fractionation, HeLa cell
Identifikasi Mutasi Gen rpob Isolat MDR Mycobacterium tuberculosis di Bali dengan Metode Nested PCR*) Identification Of rpob Gene Mutation of MDR Mycobacterium tuberculosis Isolate in Bali Using Nested PCR Yowani, Sagung Chandra; Yowani, Sagung Chandra; Wirajana, I Nengah
Jurnal Farmasi Indonesia Vol 7, No 2 (2014)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35617/jfi.v7i2.165

Abstract

ABSTRAK Tuberkulosis merupakan salah satu penyakit infeksi yang menjadi perhatian untuk ditangani pada program Millenium Development Goals (MDGs), terutama oleh meningkatnya kejadian Multi Drug Resistance (MDR_TB). Menurut WHO, resistensi terhadap rifampisin dan isoniazid telah dikategorikan sebagai MDR-TB. Dari beberapa penelitian, ditemukan bahwa hampir 90 % isolat yang resisten rifampisin, juga resisten terhadap isoniazid. Oleh karena itu,resistensi terhadap rifampisin dianggap sebagai marka penentu (surrogate marker) kejadian MDR-TB. Resistensi terhadap rifampisin dimediasi oleh adanya mutasi pada suatu region 81 bp (RRDR : Rifampicin Resistance Determinant Region) dari gen rpoB. Penelitian ini bertujuan utamanya untuk mengeksplorasi mutasi pada gen rpoB dari MDR-TB yang terjadi pada penderita Tuberkulosis di Bali. Isolat MDR-TB yang digunakan pada penelitian ini merupakanj koleksi Laboratorium Mikrobiologi RS Sanglah, Denpasar. Amplifikasi dilakukan dengan metode Nested Polymerase Chain Reaction menggunakan sepasang outer primer dan sepasang inner primer Hasil penelitian menunjukkan selain mutasi pada daerah RRDR, 5 dari 6 isolat yang dikerjakan memiliki titik mutasi yang sama yaitu pada kodon 418, dengan perubahan asam amino dari asam glutamat menjadi asam aspartat.Oleh karenanya, dapat disimpulkan bahwa terdapat missense mutation   Kata Kunci : Nested PCR, MDR-TB, Mutasi gen rpoB ABSTRACT Tuberculosis is one of the  infectious diseases that is important to be treated in Millenium Development Goals (MDGs) programme, mainly due to the increasing of Multidrug resistant tuberculosis (MDR-TB) cases. According to WHO, MDR-TB was defined as tuberculosis caused by strains of Mycobacterium tuberculosis that are resistant to at least isoniazid and rifampicin. Several studies found that nearly 90% of rifampin-resistant isolates, were also resistant to isoniazid. Therefore, resistance to rifampicin is a surrogate marker of MDR. Resistance to rifampicin is mediated by  mutation in a region of 81 bp (RRDR: Rifampicin Resistance Determinant Region) of the rpoB gene. The aim of this research mainly was to explore mutation of MDR-TBâ?? rpoB gene of tuberculosis patient in Bali. All MDR-TB isolates used for this research were the collection of Clinical Microbiology Laboratory Of Sanglah Hospital, Denpasar. Amplification were conducted by Nested Polymerase Chain Reaction methods using two pair of  inner and outer primers. The result of this study showed that except at RRDR, five of six isolates had one similar mutation at codon 418 from glutamic acid to aspartic acid. Therefore, it could be concluded that there has been a missense mutation in all isolates. Key words: Nested PCR, MDR-TB, r.poB gene mutation

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