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Rachmat Hidayat
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INDONESIA
Bioscientia Medicina : Journal of Biomedicine and Translational Research
Published by Universitas Sriwijaya
ISSN : -     EISSN : 25980580     DOI : -
Core Subject : Health, Science,
BioScientia Medicina is an open access international scholarly journal in the field of biomedicine and translational research aimed to publish a high-quality scientific paper including original research papers, reviews, short communication, and technical notes. This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to medical sciences, Traditional Herb, genetics, immunology, environmental health, toxicology, bioinformatics and biotechnology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.
Arjuna Subject : Kedokteran - Anatomi
Articles 1,209 Documents
The Association between 25-(OH)D Level and Metabolic Control Status in Children with Type 1 Diabetes Mellitus at Dr. M. Djamil General Hospital Padang Dini Anggini; Eka Agustia Rini; Finny Fitry Yani
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.530

Abstract

Background. Type 1 diabetes mellitus (T1DM) is an autoimmune disease causing the destruction of pancreatic beta cells. This is an incurable condition, but with good metabolic control, an optimal quality of life can be achieved. Glycated hemoglobin (HbA1C) is still considered a reliable parameter of metabolic control. Studies showed vitamin D has a role in controlling glycemic homeostasis in children with T1DM. Calcidiol or 25-(OH)D is the best parameter to determine the level of vitamin D in the blood. This study aimed to evaluate the association between 25-(OH)D with metabolic control status in T1DM children at Dr. M. Djamil General Hospital Padang. Methods. A cross-sectional study was conducted on 43 pediatric patients with T1DM from July 2019-January 2021. Serum levels of 25-(OH)D were measured by direct CLIA method and classified into deficiency (≤ 20 ng/mL) and insufficiency (21–30 ng/mL). The HbA1C levels were calculated using the HPLC method and classified into good (<7%), adequate (7-8%), and poor (>8%) control. The Chi-square test and ANOVA were used for data analysis. The P-value of < 0.05 was considered statistically significant. Results. The majority of respondents were girls (53.5%), with 90.7% having a good nutritional status. The mean age at diagnosis was 11.25±2.85 years, and had been known to suffer from T1DM for 2.95±1.74 years. All respondents had abnormal levels of 25-(OH)D (100%), i.e., insufficiency (28%), deficiency (72%), poor (65.1%) metabolic control, and 96.4% of respondents with poor metabolic control had a deficiency of 25-(OH)D. (P-value <0.001). Conclusion. T1DM patients who have poor metabolic control have very low levels of 25-(OH)D.
Profile of Non-Melanoma Skin Cancer and Malignant Melanoma at Dr. Mohammad Hoesin General Hospital Palembang from 2017 - 2019: A Retrospective Study Yulia Farida Yahya; Odelia Jovita Jusuf Fantoni; Susanti Budiamal; Theresia L. Toruan
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.531

Abstract

Background: Currently, there is an increasing trend in skin cancer incidence, especially non-melanoma skin cancer (NMSC), including basal cell carcinoma (BCC) and squamous cell carcinoma (SCC), also malignant melanoma (MM), causing an increase in morbidity and mortality rates. The high treatment cost causes an economic burden of disease in the country and negatively impacts public health issues. This study aimed to determine the profile of NMSC and MM regarding the sex, age, occupation, location of the lesion, clinicohistopathological diagnosis of skin cancer in Dr. Mohammad Hoesin General Hospital Palembang January 2017-December 2019. Methods: A retrospective analytical observational study obtained from medical records of NMSC and MM patients in Dr. Mohammad Hoesin General Hospital Palembang from January 2017-December 2019 as confirmed using histopathological results from the Pathology Department. Results: There were 324 new cases of NMSC and MM, including BCC 162 cases (50%) and SCC 130 cases (40.1%), whereas MM 32 cases (9.9%). Women had a higher probability of developing skin cancer with a ratio of 1.13:1. The average age of skin cancer was between 55-64 years old (33.6%), with a mean age of 57 years old. There was a statistically significant relationship between gender, age, occupation, and areas of skin cancer against skin cancer. Furthermore, overexposure to ultraviolet (UV) radiation is related to high-risk occupations, including farmers (50%), while the most frequent location of skin cancer was the face (67.3%). Conclusion: Our study implicated a 1.5-fold increase in new cases of skin cancer between 2017 to 2019. BCC cases were more prevalent than SCC and MM cases. There was also a significant association between skin cancer and occupation also the location of skin cancer as the risk factor for UV exposure. Nevertheless, further research involving larger samples or a multicenter approach is needed.
Potential of Hydrogel Acemannan Aloe Vera (Aloe vera) on Wound Healing After Tooth Extraction In vivo Via Regulation of Inflammatory Response Firdha Muharraran; Gusbakti Rusip; Rosa Paradita Dalimunthe
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.532

Abstract

Background: Tooth extraction is a surgical procedure that involves hard tissue such as bone and soft tissue. Wounds on soft tissue are then followed by a healing process that occurs through three phases, namely the inflammatory phase, the proliferative phase, and the remodeling phase. Acemannan has the ability to stimulate hematopoiesis and antioxidant effects. Acemannan is able to reduce inflammation through prostaglandin synthesis and increase leukocyte infiltration and play a significant role in the oral wound healing process. Methods: This study is an in vivo experimental study. A total of 30 rats (5 each/group) were divided into groups that received 1%, 2%, 4%, 8%, povidone-iodine, and carbopol acemannan Aloe vera hydrogel. Furthermore, the number of macrophage cells in the tissue after tooth extraction was assessed. Data analysis was carried out with the help of SPSS with ANOVA and post hoc LSD tests. Results: There was a significant difference between the application of acemannan 1% hydrogel and 4% acemannan hydrogel, 8%, povidone-iodine, and carbopol, between the application of acemannan 2% hydrogel 4% acemannan hydrogel, 8%, povidone-iodine and carbopol, applications acemannan hydrogel 8% with carbopol, and between povidone-iodine and carbopol (p≤0.05). Conclusion: Acemannan Aloe vera hydrogel is able to accelerate wound healing after tooth extraction in vivo by suppressing macrophage cell activity.
Relationship between Initial CT Value, Inflammatory Markers, and Onset of Clinical Symptoms with Outcomes of Treatment Outcomes of COVID-19 Patients at Dr. M. Djamil General Hospital Padang Irvan Medison; Chicy Widya Morfi; Elsesmita Elsesmita; Diana Nur Asrini; Hendris Utama Citra Wahyudin; Ahmad Junaidi; Jefri Sofian Leksana; Bobby Hasibuan; Adhika Rahman
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.533

Abstract

Background: Treatment given for COVID-19 includes the provision of antiviral, anti-inflammatory, immunomodulatory, anticoagulant, and treatment of comorbidities and complications. The success of treatment in COVID-19 patients does not only depend on the drug given but is also influenced by clinical manifestations, clinical phase, and the presence or absence of complications and comorbidities. This study aims to assess the relationship between initial CT values, inflammatory markers, and symptom onset on treatment outcomes for COVID-19 patients at Dr. M. Djamil General Hospital Padang. Methods: This study is an observational analytic study with a retrospective cohort approach in confirmed COVID-19 patients treated at Dr. M. Djamil General Hospital Padang between 1st January – and 31st May 2021. The sample is the population that meets the inclusion criteria; confirmed COVID-19 from the results of RT PCR/TCM SARS-CoV-2, aged > 18 years, including the clinical degree of moderate, severe, critical illness. Exclusion criteria were patients with incomplete medical record data. Univariate analysis is intended to describe each research variable so that the distribution and percentage of each variable are presented in tabular form. Bivariate analysis using Chi-Square statistical test to see the relationship between variables. Results: COVID-19 patients being treated at Dr. M. Djamil General Hospital Padang were mostly in the age group above 50 years, namely 59.6%, and the female sex was treated more than the male, namely 55.5%. The moderate clinical grade is the highest proportion of hospitalized patients, namely 64.3%, followed by critical clinical 30.0% and severe clinical 5.6%. There is no relationship between the initial CT value and the outcome of COVID-19 patients. There is a relationship between IL-6 levels (initial) with the outcome of Covid-19 patients at Dr. M. Djamil General Hospital Padang. There is no relationship between the onset of clinical symptoms and the outcome of Covid-19 patients at Dr. M. Djamil General Hospital Padang. Conclusion: There is a relationship between IL-6 levels (initial) with patient outcomes, need for oxygen therapy, ferritin levels with the incidence of sepsis, initial albumin levels with initial D-Dimer levels, and IL-6 levels with D-dimers in COVID-19 patients at Dr. M. Djamil General Hospital Padang.
The Effect of Ethanol Extract of Kersen Leaf (Muntingia Calabura L.) on Reducing Triglyceride Levels in Hypercholesterolemic Rats Imaniar Ranti; Nur Vickasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.534

Abstract

Background: Kersen leaf has the potential to lower triglycerides because they contain flavonoids and tannins. Methods: This study was an experimental laboratory study with a pre and post-test with a control group design. The subjects were 30 white male rats (Rattus norvegicus), bodyweight ±200 grams, aged 3-4 months, and divided into 5 groups with random sampling. The groups were negative control (K-) was given a high-fat diet, and PTU without any treatment, positive control (K+) was given a high-fat diet, and PTU with gemfibrozil treatment, (P1),(P2),(P3) was given a high-fat diet and PTU with ethanol extract of Kersen leaf 100, 200, and 400 mg/kgBB. This study was held for 4 weeks. Triglyceride level was measured using the GPO-PAP method. The data was analyzed using Shapiro-Wilk, Levene test, and ANOVA test. Results: The ethanol extract of Kersen leaf dose 100, 200 and 400 mg/kgWB are found effective on reducing blood triglyceride levels with a mean reduction in the first treatment of 112,83 mg/dl, 98,20 mg/dl, 89,92 mg/dl and in the second treatment of 106,71 mg/dl, 93,95 mg/dl dan 76,87 mg/dl. Statistical analysis using one-way ANOVA showed p<0,05, which determines a significant difference in triglyceride levels among each group. Conclusion: Extract dose of 400 mg/kgWB is found to be the most effective dose in triglyceride reduction.
The Effect of Ethanolic Extract of Moringa oleifera Leaves on the Macrophage Count and VEGF Expression on Wistar Rats with Burn Wound Agnes Stella Valentina; Najatullah; Trilaksana Nugroho; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.535

Abstract

Background: One modality type of burn wound therapy is topical silver sulfadiazine. Recently, research about topical herb medicine has grown. Moringa oleifera (MO) is a kind of herb plant that has an anti-inflammation effect and has an influence on angiogenesis, so it tends to faster burn wound healing. This study aimed to prove the effect of ethanolic extract of Moringa oleifera leaves on the macrophage count and VEGF expression in Wistar rats with burn wounds. Methods: The research design that was used is "Randomized post-test with a control group." The study population used 24 male Wistar rats that were induced burn wounds and randomly divided into 4 groups which were given medicine topically once daily for 7 days. Treatment groups included: I (MO leaves extract 10%), II (SSD + MO leaves extract 10%), III (SSD), dan IV (pure vehiculum). Exudation was assessed macroscopically, while PMN and macrophage amount were assessed microscopically with Hematoxylin-Eosin. Data were analyzed and processed using hypothesis test One Way ANOVA – Post Hoc Bonferroni, Kruskal Wallis – Mann Whitney, and correlation test Spearman and Pearson with SPSS 25.0 program. Results: Significant difference obtained in macrophage amount between II group and IV group (p 0,000). There was no significant difference in VEGF expression. There was also no correlation between macrophage amount and VEGF expression. Conclusion: Ethanolic extract of Moringa oleifera leaves extract proved to be effective in decreasing macrophage cell count on Wistar rats with burn wounds.
The Effect of Ethanolic Extract from Moringa oleifera Leaves in Collagen Density and Numbers of New Capillary Vessel Count on Wistar Rats Burn Wound Supandi Andy; Najatullah; Nugroho Trilaksana; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.536

Abstract

Background: Burns are a major cause of morbidity, including prolonged length of stay and disability, which requires no small amount of treatment costs. Currently, there are many studies to accelerate the healing of wounds, one of which is with Moringa oleifera (MO). This study aimed to prove the effect of ethanolic extract from MO leaves on collagen density and new capillary vessel count on Wistar rats' burn wounds. Methods: This research is randomized post-test only with a control group design. Twenty-four male Wistar rats induced burn wounds and were randomly divided into 4 groups which were given topical medicine once daily for 10 days; P1 (MO leaves extract 10%), P2 (Silver Sulfadiazine + MO leaves extract 10%), P3 (SSD), and P4 (negative control). Collagen density was assessed using the Kruskal-Wallis test and continued with Mann-Whitney Test. New capillary vessel count was assessed using Hematoxylin-Eosin staining and One Way ANOVA. The correlation test between two variables was tested using the Spearman test. Results: Collagen Density shows significant difference in group P1 compared to P4 (p=0,016), group P2 compared to P3 (p=0,047), group P2 compared to P4 (p=0,009). A significant increase in new capillary blood vessel counts was seen in group P1 compared to P3 and P4 (p=0.001; 0,000) and group P2 compared to P3 and P4 (p=0,000;0,000). A positive correlation was found between the new capillary blood vessel count and epithelization percentage (p=0,001), with a strong correlation (rho=0,682). Conclusion: Ethanolic extract from Moringa oleifera leaves proved to be effective in increasing collagen density and new capillary vessel count.
Cortisol Level Related to Depression in Women with Endometriosis Adnan Abadi; Aria Indrabrata; Iskandar Zulqarnain; Theodorus; Abdullah Sahab; Kemas Yusuf Effendi; Heriyadi Manan; Fatimah Usman; Awan Nurtjahyo; Cindy Kesty
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.537

Abstract

Background: Several main symptoms of endometriosis can lead to physical and psychological stress and also hormonal disturbances as the result of prolonged stress. Cortisol was associated with the onset of depression. This study aimed to determine the relationship between cortisol levels and depression in women with endometriosis at the Department of Obstetrics and Gynecology at Dr. Mohammad Hoesin General Hospital (RSMH) Palembang. Methods: A cross-sectional study was performed at the Reproductive Endocrinology and Infertility outpatient clinic and inpatient ward, Department of Obstetrics and Gynecology at Dr. Mohammad Hoesin General Hospital Palembang, from January to November 2020. There were 74 samples of endometriosis patients who met the inclusion criteria. The blood cortisol level was examined using the ELISA method. The determination of depression level was performed by filling out the Beck Depression Inventory questionnaire. Data were analyzed using SPSS version 22.0. Results: Seventy-four samples met the inclusion criteria. As many as 44 patients (59.5%) without depression, 20 patients (27%) with mild depression, 8 patients (10.8%) with moderate depression, and only 2 patients (2.7%) with severe depression. There were no differences in age, marital status, parity, menstrual cycle, and duration of treatment between endometriosis patients with and without depression (p > 0.05). There was a significant difference in cortisol levels between endometriosis patients with and without depression (p = 0.017). Endometriosis patients with cortisol levels ≤ 7.4 mg/dl were three times more at risk of depression than endometriosis patients with cortisol levels > 7.4 mg/dl. In addition, there was a significant relationship between cortisol level and depression status (OR = 3.023 (95% CI 1.153–7.942; p = 0.041). This study also found a significantly low negative correlation between cortisol levels and BDI scores (r = -0.249; p = 0.032). Conclusion: There was a significant relationship between serum cortisol levels and depression in women with endometriosis. Endometriosis patients with depression had significantly lower cortisol levels than endometriosis patients without depression.
Profile of p53 Protein Expression in Basal Cell Carcinoma Low-Risk and High-Risk Subtype Alendra Chakramurty; Meira Dewi Kusuma Astuti; Hermawan Istiadi; Awal Prasetyo; Dik Puspasari
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 6 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i6.538

Abstract

Background: Basal cell carcinoma is one of the most common cancers in the world, and its incidence is increasing over time. It is estimated that it accounts for three-quarters of all non-melanoma skin cancers, and the incidence increases exponentially in predominantly white areas. The p53 gene is the gene that most undergoes mutations due to sun exposure and is the cause of skin cancer, especially basal cell carcinoma. This finding explains that p53 expression can be used as marker analysis in cases of basal cell carcinoma. Methods: Observational analytic design was carried out with a cross-sectional. The population and samples were paraffin blocks which histopathologically diagnosed Basal Cell Carcinoma in the anatomical pathology laboratory, Dr. Kariadi General Hospital Semarang. Samples were processed by the p53 immunohistochemical staining method. The p53 expression was given a quantitative score to be 0 = negative, 1-2 = low, 3-4 = high. The expression of p53 was considered positive when it was colored in the cell nucleus. The correlation between variables was analyzed by Spearman and Mann Whitney correlation test. Results: There is no correlation between p53 expression and the age of p53 patients with p = 0.390 (p > 0.05). There is no correlation between p53 expression and lesion location with p = 0.817 (p > 0.05) There is no difference significant, between p53 expression and gender with p = 0.576 (p > 0.05). Conclusion: There is no correlation between high and low-risk Basal Cell Carcinoma subtype associated with age, gender, and lesion location.
Knowledge and Attitudes of Pregnant Women Toward Iron Supplementation During Pregnancy in the Work Area of Pegasing Health Center Central Aceh in 2021 Hidayana; Sri Wahyuni MS; Nova Ratna Dewi
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 7 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i7.540

Abstract

Background: This study aims to determine the extent of knowledge and attitudes of pregnant women toward the consumption of iron tablets in the working area of Pegasing Health Center, Pegasing District, Central Aceh Regency, in 2021. Methods: This research is descriptive research. The research sample was 35 pregnant women in the working area of Pegasing Health Center. Data were collected and measured using a questionnaire of 40 questions, 20 questions about knowledge, and 20 questions about attitudes. Results: The mother's knowledge about the definition and need for iron is in the sufficient category (41.86%), the impact of iron deficiency is in a good category (74.29%) the purpose of giving iron is in the good category (57.14%) containing a source of iron is in the sufficient category (65.71%) while the attitude of pregnant women to the consumption of iron tablets is in a good category (42.86%). Conclusion: Most of the respondents have a sufficient level of knowledge regarding the definition, benefits, and food sources of iron during pregnancy. Most pregnant women are well aware of the impact of iron deficiency and the purpose of iron tablet supplementation during pregnancy. The majority of respondents have a good attitude towards iron supplementation during pregnancy.

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