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Jurnal Ilmu Dasar

JID
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Published by Universitas Jember

ISSN : 24425613 EISSN : - DOI : https://doi.org/10.19184/jid.v24i2.36657

Core Subject : Education, Engineering,

Control & Systems Engineering Mathematics

Jurnal ILMU DASAR (JID) is a national peer-reviewed and open access journal that publishes research papers encompasses all aspects of natural sciences including Mathematics, Physics, Chemistry and Biology. JID publishes 2 issues in 1 volume per year. First published, volume 1 issue 1, in January 2000 and avalaible in electronically since 2012 with ISSN 1411-5735 (Print) and avalaible in electronically since 2012 with ISSN 2442-5613 (online). Jurnal ILMU DASAR is accredited SINTA 3 by the Ministry of Education, Culture, Research, and Technology of the Republic of Indonesia (Kemendibukristek) No. 152/E/KPT/2023 (September 25, 2023), Ministry of Research, Technology and Higher Education of the Republic of Indonesia (RISTEKDIKTI), No. 200/M/KPT/2020 (December. 23, 2020). All accepted manuscripts will be published worldwide JID has been indexed in DOAJ, Dimension, OCLC WorldCat, PKP Index, Crossref, Google Scholar, Base, Garuda, and OneSearch. JID have been collaborated in KOBI-ID (Konsorsium Biologi Indonesia) and HKI (Himpunan Kimia Indonesia) since 2017.

Arjuna Subject : Matematika - Kontrol dan Optimasi

Articles 11 Documents

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Search results for , issue "Vol 21 No 1 (2020)" : 11 Documents clear

The Application of UTY and SRY Molecular Markers for Determination of Unknown Sex Samples in Bali Cattle Indriawati, Indriawati; Volkandari, Slamet Diah; Margawati, Endang Tri
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

An investigation involving large number of animals is often resulting incomplete or in accurate information such as animal parentage, or misidentify on sex due to unlabeled sex samples. A PCR method by applying Y chromosome markers (UTY and SRY) facilitates in determination of unknown sex problem. This study was intended to determine sex from unlabelled sex of blood samples by applying PCR method using a pooled-DNA template. Twenty five of unknown sex blood samples from Nusa Penida, Bali were used in this study. The samples were plotted into 5 pooled-DNA whith each pool DNA consisted of 5 individuals DNA. Two pairs of sex primers, UTY (58oC) and SRY (60oC) with 35 cycles were applied to amplify the samples. The result showed there was only one pooled-DNA (P4) amplified by UTY (484bp). Whereas re-PCR of the positive pooled-DNA (P4) using SRY primer, only one out of 25 samples determined as male Bali cattle (325bp). This finding suggests that UTY and SRY primers are suitable for sex determination and the pooled-DNA could be used as an efficient PCR method both in consumables and PCR process for sex determination. Keywords: Determination, sex, unknown sample, pooled DNA, Bali cattle.

Analysis of Secondary Metabolites of Shoot, Callus Culture and Field Plant of Chrysanthemum morifolium Ramat Setiawati, Tia; Ayalla, Alma; Nurzaman, Mohamad; Kusumaningtyas, Valentina A.; Bari, Ichsan
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

The chrysanthemum plant (Chrysanthemum morifolium Ramat.) contains many secondary metabolites such as flavonoids and various volatile compounds that can be utilized as drugs. Tissue culture can be an alternative to enhance the production of certain secondary metabolite. The study aimed to determine the types of secondary metabolites that contained in shoot culture, callus and field plants of C. morifolium. The research method was exploration in the laboratory to analyze and compare the content of secondary metabolite from shoot culture, callus and field plants of C. morifolium. Callus was induced by explants of C. morifolium plantlet stems and leaves respectively on MS medium with an addition of 3 ppm 2,4-D + 2 ppm kinetin and 4 ppm 2,4-D. For shoot culture, single nodule explants with one leaf were planted on MS media with the addition of 1 ppm BAP. The secondary metabolite compouds were analized and identified by GC-MS (Gas Chromatography-Mass Spectrometry). The results showed that various types of secondary metabolites contained in shoot culture, callus and field plants of C. morifolium. In callus culture from leaf explants, four compounds from groups of alcohol, acetic acid and organosilicon were identified, whereas in callus culture from stem explants were identified eight compounds from aldehydes, esters, alkanes, and carboxylic acids group. In the shoot culture, nine compounds of alcohol, ketone, aldehyde, cycloalkane and organosilicon group were identified, while in the field plants five compounds were identified from the cycloalkanes, ketones, organoborones and organosilicon group. Some detected compounds have a potential as precursors of alkaloid, phenolic, and flavonoid.Keywords: chrysanthemum, culture, shoots, callus, secondary metabolites.

Effect of Potassium Fertilization on Growth and Head Rice Yield of Rice (Oryza sativa L.) Merah Wangi Variety Fanata, Wahyu Indra Duwi; Usmadi, Usmadi; Ramadhan, Galang Rizki
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

The fertilization management is one strategy to optimize the quantity and quality of rice production. Our study was aimedto investigate the effect of potassium fertilization several agronomical parameters andthe rate of head rice after milling process. The study was conducted from March 2018 to July 2018 in the Agrotechnopark experimental field University of Jember in District of Sukorambi, Jember. Randomized Block Design (RBD) was used with the frequency of potassium chlorice (KCl) fertilization asthe single factor. The conducted potassium treatments were as follows: without KCl fertilization (control), one-time KCl fertilization, two times KCl fertilization, three times KCl fertilization and each fertilization level was conducted by sixrepetitions. The agronomic parameters such as plant height, tiller number, flowering time, rate of productive tillers, grain number per panicle, weight of 1000 grains, and percentage of filled grain were analyzed and percentage of head rice rate was determined as quality parameter. Our results showed that different treatment of potassium did not significantly affect the plant height, tillernumber, flowering time, rate of productive tillers, total grains per panicle, and weight of 1000 grains. On the other hands, the percentage of filled grainand the rate of head rice after milling were significantly increased by potassium fertilization. Compare to the control treatment, three times potassium fertilization resulted 9.24% and 13.83% increase of filled grain and head rice after two times milling, respectively. Our result suggest that potassium fertilization is important for grainquality of Merah Wangi rice variety. Keywords: Merah Wangi, potassium fertilization, growth, physical quality.

Food Habit of Seluang Batu (Paracrossochilus vittatus Boulenger 1894) in Mentuka River Sekadau Districts West Kalimantan Province Pratiwi, Diah; Setyawati, Tri Rima; Yanti, Ari Hepi
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Seluang batu (Paracrossochilus vittatus) is one of the Borneo endemic fish from Cyprinidae Family that live in high stream water. The aim of this study was to know the food habit and niche breadth of seluang batu in Mentuka River. This study was conducted 3 months from November 2016 to January 2017. The sampling method used in this research was purposive random sampling. Seluang batu was taken from three stations in the Mentuka River using trap nets. The fish was measured in length and weight, then dissected to find out the type of food in the stomach. Food analysis was determined using index of preponderance and the niche breadth using Smith’s index. The results showed that seluang batu in Mentuka River including herbivore because they eat microalgae, namely Synedra was main food. Nice breadth of seluang batu for peryphiton was 0.71 meanwhile plankton was 0.20. Most of peryphiton and plankton in Mentuka River are Bacillariophyceae. The Mentuka River environtment supported the life of seluang batu and their natural food, namely the temperature about 26-28 ̊C, current speed was about 1-2,6 m/s, pH was about 6,8-7,3, dissolved oxygen was about 6,1-7 mg/L and free CO2 was 3,9-5,0 mg/L. Keywords: food habit, Paracrossochilus vittatus, periphyton, plankton.

The Expression of The PfEMP1-DBL2β Recombinant Protein of Plasmodium falciparum Isolated From Indonesia Hasanah, Fathul Hidayatul; Sulistyaningsih, Erma; Sawitri, Widhi Dyah
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

The binding of Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) to Intracellular Adhesion Molecule-1 (ICAM-1) is a major pathological mechanism in severe malaria including cerebral malaria. The binding is mediated by PfEMP1-DBL2β domain. The study aimed to explore there combinant protein of PfEMP1-DBL2β domain of P. falciparum isolated from Indonesia. DNA was isolated from a severe malaria patient. The DBL2β domain was amplified using Polymerase Chain Reaction (PCR) with specific primer and cloned into the pJET1 cloning vector. The DBL2β recombinant protein was constructed from DBL2β-pJET1 clone using pET-30a expression vector and expressed in Escherichia coli BL21-DE3. PCR colony and digestion of plasmid clones using restriction enzymes were conducted to confirm cloning result, and the expression of recombinant protein was analyzed using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The expression of DBL2β-PfEMP1 domain is higher in pellet than in supernatant fraction. In conclusion, the DBL2β-PfEMP1 domain recombinant protein of P. falciparum isolated from Indonesia expressed as a ~66 kDa protein in full length. Keywords: DBL2β domain, Indonesia, PfEMP1, Plasmodium falciparum, recombinant protein.

The Polymorphic Gene of Single Nucleotide Polymorphism (SNP) of Phytoene Synthase (PSY) to Characterize Carotenoids Yellow Root Cassava Kurniawati, Siti; Hartati, N. Sri; Hartati, Hartati; Sudarmonowati, Enny
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Cassava (Manihot esculenta Crantz.) is a carbohydrate sources containing a limited amount of micronutrients, but some genotypes contain β-carotene as the precursor of vitamin A in the storage roots and leaves. Improvement of β-caroteneand minerals such as Fe / Zn content of cassava’s nutrition is mostly through by biofortification program. The storage root of β-carotene recognized by a yellow or yellowish color while the apical shoots with red to purplish. β-carotenein carotenoid biosynthetic pathway is an expression of the phytoene synthase (PSY) gene. The MePSY2 gene, one of the three MePSY family is the key gene to characterize carotenoids related gene in cassava. In this study, sequencing of the two cassava fulllenght PSY genomic DNA was carried out in conserved areas in the PSY gene region (PSY1 and PSY2) from the DNA of the cassava leaves. Adira1, Carvita25 and Ubi Kuning are yellow root storage genotypes (K1, K2 and K3) while Adira4 and Menti are white root storage genotypes (P1 and P2). Carvita25 is induced somaclonal variant of the Adira4 genotype. Contiq and consensus of nucleotide base sequences from the five cassava genotypes and CM3306-4 cultivars (acc GU111715.1) as references were analysed using the lasergene DNASTAR sequence analysis program. The results of the alignment of the base sequence constituent of the MePSY2 gene showed that the PSY2 gene with amplified genome length was 2,380 base pairs (bp) consisting of 1,140 bp exon region and 1,240 bp intron region. In the conserved coding region, there was a difference of one nucleotide base, that is, base C in two white tuber cassava genotypes replaced with A in three yellow tuber cassava genotypes in the 1.485 base (C1.485A). The SNP converts the amino acid (aa) alanine (A) to aspartic acid (D) at the 191th (A191D). Single Nucleotide polymorphism in conserved coding region can be used further as carotenoid marker for plant breeding of yellow root cassava. Keywords: β carotene, PSY gene, polymorphic gene SNP, yellow root cassava.

Cover JID Volume 21 No 1 (2020) DASAR, Jurnal ILMU
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Determining Flood Protection Strategy with Uncertain Parameter Using Adjustable Robust Counterpart Methodology Chaerani, Diah; Robbi, Muttaqien Rodhiya; Hertini, Elis; Rusyaman, Endang; Paulus, Erick
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Flooding is a natural disaster that often occurs, it is not surprising that floods are one of the problems that must be resolved in various countries, one of which is Indonesia. Flood is very detrimental to the public because the impact could be the loss of material and non-material. A flood protection system is needed and must be managed properly. This aims in management of flood protection systems often requires efficient cost control strategies that are the lowest possible long-term costs, but still meets the flood protection standards imposed by regulators in all plans. In this paper a flood protection strategy is modeled using Adjustable Robust Optimization. In this approach, there are two kinds of variables that must be decided, i.e., adjustable and non-adjustable variables. A numerical simulation is presented using Scilab Software. Keywords: Flood Protection Strategy, Uncertainty, Adjustable Robust Optimization, Scilab Software.

Optimization of Povidone K-30 and Sodium Starch Glycolate on Levofloxacin Tablet by Factorial Design Setyawan, Dwi; Soeratri, Widji; Nuruddin, Mahrus Naufal; Paramita, Diajeng Putri; Widjaja, Bambang
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

The aim of this study was to determine the effect of binder and disintegrant excipients toward tablet properties of levofloxacin as the latter tends to suffer brittle fracture upon compression. The excipients used were povidone K-30 as the binder and sodium starch glycolate (SSG) as the disintegrant which the tablets were formulated according to factorial design 22 with two factors and two levels on each factor. Four formulas were prepared by wet granulation method using 2 and 4% of each povidone K-30 and sodium starch glycolate in various compositions. Tablet properties were evaluated for its hardness, friability, and disintegration time as well as dissolution profile. The data obtained was statistically analyzed using Minitab® 17 software to optimize the formulation and resulted in different impacts caused by each excipient. Povidone K-30 exhibited an increment in hardness, friability, disintegration time but a decrease indissolution profile of levofloxacin tablet. SSG decreased hardnessand disintegration time, but increased friability and dissolution profile of levofloxacin tablet. Overlaid contour plot showed that the optimal formula regarding tablet properties of friability, disintegration time, and dissolution profile is in composition of 2.01% povidone K-30 and 2.01% sodium starch glycolate. Keywords: levofloxacin tablet, povidone K-30, sodium starch glycolate, factorial design.

Humic Acid Isolation from Batujai Dam Lombok Tengah-NTB and its Potential as Reductive–Biosorbent Au(III) in The Batch System Ismillayli, Nurul; Hermanto, Dhony
Jurnal ILMU DASAR Vol 21 No 1 (2020)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

The Study of Au(III) ions adsorption by humic acid in batch system has been done. Humic acid was isolated from Batujai Dam Lombok Tengah 0.1 M NaOH and continued with 0.1 M HCl/0.3 M HF washing to dissolve silica. Functional groups of isolated humic acid was investigated by FTIR Spectroscopy and the adsorption process was studied by XRD and SEM analyses. It has been found that humic acid contained carboxylic, phenols, alcoholic, ether, amines functional groups, aliphatic hydrocarbon and aromatic ring. XRD and SEM data showed that humic acid did not only adsorp Au(III) ions but also reduce them to gold particles. Therefore, humic acid has the potential as reductive-biosorbent for Au(III) in batch system. Keywords: humic acid, reductive-biosorbent, Au(III) ions.

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Home Page

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Contact Name Solikhatun

Contact Email solikhatun@ugm.ac.id

Phone +62331334293

Journal Mail Official jid@unej.ac.id

Editorial Address Jl. Kalimantan 37 Kampus Tegalboto Jember 68121 Telp. 62 331 334293 Fax. 62 331 330225

Location Kab. jember, Jawa timur INDONESIA

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Contact Name
Solikhatun

Contact Email
solikhatun@ugm.ac.id

Phone
+62331334293

Journal Mail Official
jid@unej.ac.id

Editorial Address
Jl. Kalimantan 37 Kampus Tegalboto Jember 68121 Telp. 62 331 334293 Fax. 62 331 330225

Location
Kab. jember,

Jawa timur

INDONESIA