Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Veterinary Biomedical and Clinical Journal

vbcj
Website

Published by Universitas Brawijaya

ISSN : - EISSN : 26221012 DOI : -

Core Subject : Health, Science, Education,

Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary

Veterinary Clinical and Biomedical Journal (VBCJ) is periodically peer reviewed journal that publish every January and July per year since 2019. VBCJ accommodate any articles in the field of veterinary medicine, biomedical, as well as veterinary case report.

Arjuna Subject : -

Articles 6 Documents

Search results for , issue "Vol. 1 No. 1 (2019)" : 6 Documents clear

Detection of Staphylococcus aureus Biofilm from Subclinical Mastitis Milk Lesmana, Muhamad Arfan; Qosimah, Dahliatul; Murwani, Sri
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

One of S.aureus's virulence factors is biofilm formation. When biofilms are formed, the bacteria will undergo phenotypic changes that require higher concentrations of antibiotics to inhibit proliferation. Phenotypic changes will lead to increase the production of extracellular matrix and multilayered colonies as well as decrease of metabolic rates, multiplication and polymicrobial colonization resulting in recurrent infection in the host and difficulty being treated with antibiotics due to resistance. The aim of this research was to know the formation of bacterial biofim by slime and quantitative by microplate titer method. The research method was qualitative descriptive using 27 samples of Staphylococcus aureusÂ with characterized from mastitis infected milk. The bacteria were grown on CRA (Congo Red Agar) media to observe the slime biofilm through bacteria black colony followed by MicrotiterPlateÂ methodÂ with 570nm wave lenght. The results showed that 27 samples of Staphylococcus aureus which positive to form slime biofilm were 10 samples and continued to microtiter plate showed 3 positive samples of biofilm. The conclusions of this study, Staphylococcus aureus in subclinical mastitis milk samples were positive to form biofilms.Â

Antioxidant Activity of Goat Milk Yogurt Casein on Dioxin Intoxicated Rat Liver Function Setianingrum, Ani; Padaga, Masdiana Chendrakasih
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Dioxin is toxic contaminant which persistent in the environment. This compound bio-accumulate through food chain and generate adverse effect in human health. Oxidative stress due to dioxin exposure can interfere metabolism process and leads to the development of hepatotoxicity.The aim of this study was to asses the role of casein derived from goat milk yogurt in dioxin-intoxicated rat based on liver function parameters. Substances used were: 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) diluted in corn oil and casein from goat milk yogurt. The study included 24 Wistar rats divided into 6 groups: (1) control group, (2) placebo group which given casein 600 mg/kg BW, (3) TCDD group given with TCDD 100 ng/kg BW, (4) group P1 given casein 300 mg/kg BW and TCDD, (5) group P2 given casein 600 mg/kg BW and TCDD, (6) group P3 given casein 900 mg/kg BW and TCDD. The respective dose were given orally for 21 days. Blood serum was collected for transaminases (ALT, AST) and Î³-glutamyl transferase (GGT) analysis. Based on serum transaminases activity, there were no significant change on AST and ALT level. Casein yogurt supplementation has significant effect on serum GGT level (p<0,05) and optimum dose was 600 mg/kg BW/day

Detection of Hydroureter in Rat After Medial and Distal Unilateral Ureteral Obstruction Using Ultrasound Frequency 10 MHz Rickyawan, Nofan; Arifianto, Dinar; Adji, Dhirgo
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Unilateral ureteral obstruction can occur in all animals and it is located proximal, medial and distal. Obstruction causes abnormalities in ipsilateral ureters in the form of hydrouterers. The aim of this study was to detect hydroureters in post-unilateral medial and distal ureteric rats using a 10 MHz ultrasonography frequency. Thirty-six female Sprague Dawley rats aged 3 months were divided into 3 treatment groups. Each group consisted of 12 individuals. Group I as a control received laparotomy treatment. Group II laparotomy with medial right-hand ureteral ligation and group III with right distal ureteral ligation. Three rats taken randomly from each group at weeks 1, 2, 3 and 4 post-ligation is then performed ultrasonography. The results of ultrasonography were analyzed descriptively, comparatively but ureteral diameter were analyzed using statistical of 3x4 factorial patterns. Ultrasound examination showed that ligation in one ureter caused rat undergoing unilateral ureteral obstruction and hidroureter. The diameter of the ureter with a hydroureter has increased from the 1st to 4th week. The increase occurs because the urine in the lumen cannot be excreted. The contralateral ureter remains normal and cannot be observed. The conclusion of the study is that hydroureter in rats can be detected using the frequency of 10 MHz ultrasonography which is characterized by anekoic tubular formation posterior to the right kidney. The unilateral ureteral obstruction has no effect on the contralateral ureter because it is still able to compensate for the performance of the ipsilateral ureter that has a hydroureter. The difference in the location of ligation and time of examination affect the increase in ipsilateral ureteral diameter.

The Potency of Pluchea indica Less as Preventive Effort to Lead (Pb) Pertubation on Rats (Rattus norvegicus) Organs Aulanni'am, Aulanni'am; Julianto, Andri; Dewi, Made Artari; Dirgahariyawan, Tri Cahyo; Mahdi, Chanif; Wuragil, Dyah Kinasih; Herawati, Herawati
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Lead (Pb) is one of the heavy m metals polluting the environment which is harmful to t the organism. Pb accumulation triggers free radic cals and disrupts the body's metabolism. Pluche indic ca leaf extract is reported contain flavonoids whiich act as antioxidants and can counteract free radiccal attacks. The purpose of this study was to de etermine the role of Pluchea indica leaf extract preve entive therapy in counteracting free radicals due too exposure to Pb in rats (Rattus norvegicus). Wistar mmale rats aged of 8-10 weeks with a body weight of about 200-250 grams. Rats were divided into 5 tr reatment groups, were group A as negative contr rol, group B as positive control induced by lead (Pb)) 10 mg/rat/day, groups C,D,E given with ethano olic extract of Pluche indica leavesof 200 mg/kgBW, , 400 mg/kgBW, and 600 mg/kgBW and lead (Pb b) exposure of 10mg/ rat/day. Kidney, liver and jejunaal histopathology using HE staining was observed microscopically. The results showed that the administtration of Pluche indica leaves extract could preve ent tissue damage to the kidney, liver and jejunum in rats exposured to Pb. Dose of 600 mg/kgBW show wed histopathological improvement in kidney, liver annd jejunum. The greater the dose, the better prev ention of tissue damage as indicated by tissue condition resembling those of control rats. The conclusion of this study, the Pluchea indica leaf extract has the potential to prevent organ damaged exposed to lead.

The Effect of Triponyl Sulphate on Fetuses Development and Placental Abnormalities in Inducing Preeclampsia of Rattus norvegicus animal model Purwatiningsih, Wawid; Aryani, Dhita Evi; Vidiastuti, Dian; Oktanella, Yudit; Firmawati, Aulia
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Preeclampsia is one of the obstetrical problems that can cause maternal and fetal morbidity and mortality. Preeclampsia causes the fetus born prematurely and low fetal weight. This is caused by high blood pressure which causes decrease of blood delivery to the placenta, so the supply of oxygen and food to the fetus decreases. As a result, fetal development inhibits and trigger born prematurely. More fatal, this disease cause the release of placental tissue from the uterus prematurely. The aim of this study was to determine the effect of administration of triponyl sulfate as induction of increased blood pressure in preeclampsia animal models, fetal development with alizarin red staining and placental abnormalities. The experimental animals were rats Rattus norvegicus mated with male rats monomating , 4 months old and 250-300 grams body weigh. Pregnant female rats were induced by triponyl sulfate 70 mg / kg BW (k +) and without induced by triponyl sulfate (k-). The results of the study showed that there were formation of the sternal bone in k- and malformation of the sternum bone at k +. Placental abnormalities occured in k +, it could be seen in the presence of ghos villi in blood vessel abnormalities in the preeclampsia placenta caused by there was no invation of trophoblast cells in the whole or partial spiral arteries and the mean of blood pressure increased.

The Effects of Plant Leaves Variants from The Philippines on Infective Oesophagustonum dentatum Larvae G. Beltran, Maria Asuncion; Robertson, Allan; J. Martin, Richard
Veterinary Biomedical and Clinical Journal Vol. 1 No. 1 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Nine aqueous extracts of plant leaves from the Philippines were evaluated against Oesaphagustomum dentatum sensitive (ODSEns) larvae for their anthelmintic effects. The plant species evaluated were Ananas comosus, Chrysophyllum cainito,Gliricidia sepium, Annona squamosa, Moringa oleifera, Tinospora rumphii Boerl,Azadirachta indica, Trichantera gigantea and Leuacaena leucocephala. The leaves of the plants gathered from Camiling, Tarlac, Philippines, were dried under room temperature for 30 days and then ground. Aqueous extracts of these plant leaves were prepared using migration buffer. In vitro migration and inhibition assays were used to evaluate the anthelmintic activity against exsheathed third stage larvae of Oesophagustomum dentatum. The experiments were put in 24-well plates at room temperature with four replications per treatment using different concentrations of 1, 2.5, 5 and 10 mg/ml. About 120 larvae per well were deposited on a larval migration apparatus consisting of 20 Î¼m nylon mesh filters and incubated 20 hours under different treatments and another 2 hours to allow the migration of active motile larvae. The evaluation criteria were based on the average numbers of inhibited larvae under the different treatments. The percentage inhibition were statistically analyzed using Two-Way ANOVA (P=â‰¤.0001) of the Graph Pad Prizm software (V5 San Diego, Ca). The most effective leaves with their highest inhibition level were from Ananas comosus (pineapple, 86.50%), Gliricidia sepium (madre de cacao, 84.50%), Chrysophyllum cainito (star apple, 83.75%), Moringa oleifera (horseradish,58.75%), Annona squamosa (sweet sop, 56.00%), Tinospora rumphii Boerl ( \Makabuhay, 55.75%), and Azadirachta indica (Neem, 51.50%), whereby extremely significant (PË‚0.001) effect against O. dentatum larvae. Two leaves extracts, from Trichantera gigantea and Leuacaena leucocephala have no significant effect on O. dentatum larvae. The larval migration assay allows initial studies on the anthelmintic effects of the herbs.

Page 1 of 1 | Total Record : 6

Filter by Year

2019 2019

Filter By Issues

All Issue Vol. 7 No. 1 (2025): Vol. 7 No. 1 2025 Vol. 6 No. 2 (2024): Vol. 6 No. 1 (2024) Vol. 5 No. 2 (2023) Vol. 5 No. 1 (2023) Vol. 4 No. 2 (2022) Vol. 4 No. 1 (2022) Vol. 3 No. 2 (2021) Vol. 2 No. 2 (2020) Vol. 1 No. 2 (2019) Vol. 1 No. 1 (2019) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Dr. Masdiana C. Padaga, DVM. M App.Sc

Contact Email
vbcj@ub.ac.id

Phone
-

Journal Mail Official
nofanvet@ub.ac.id

Editorial Address
-

Location
Kota malang,

Jawa timur

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Dr. Masdiana C. Padaga, DVM. M App.Sc

Contact Email vbcj@ub.ac.id

Phone -

Journal Mail Official nofanvet@ub.ac.id

Editorial Address -

Location Kota malang, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Dr. Masdiana C. Padaga, DVM. M App.Sc

Contact Email
vbcj@ub.ac.id

Phone
-

Journal Mail Official
nofanvet@ub.ac.id

Editorial Address
-

Location
Kota malang,

Jawa timur

INDONESIA