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Veterinary Biomedical and Clinical Journal
Published by Universitas Brawijaya
ISSN : -     EISSN : 26221012     DOI : -
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary
Veterinary Clinical and Biomedical Journal (VBCJ) is periodically peer reviewed journal that publish every January and July per year since 2019. VBCJ accommodate any articles in the field of veterinary medicine, biomedical, as well as veterinary case report.
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Articles 7 Documents
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Search results for , issue "Vol. 1 No. 2 (2019)" : 7 Documents clear
The Routine Histopatological Examination-Chicken with Suspect Coryza (Stress Related Disease) Haryo, Albiruni; Enola, Janice
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2854.206 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.2

Abstract

Coryza (snot) kown as respiratory disease in chickens caused by Haemophilus paragalinarum. Coryza is a disease that often occurs and results in large economic losses impact. The aim of this study was to look and diagnose changes in macroscopic and microscopic (histopathology) in chickens that have been examined and suspected of Coryza (snot) disease. The organs examined are palpabrae (eyes), trachea, pancreas and cerebellum. Examining and observing macroscopic changes, swelling of the palpabrae, tracheal hemorrhages and pancreatic hemorrhages and cerebellum were normal. Microscopic changes seen in palpabrae are oedema, hemorrhage and blood clot found, tracheal epithelial erosion, hemorrhage, multiplication of goblet cells and inflammatory infiltration of PMN cells, pancreas shown  there is an inflammatory cell infiltration and fibrosis necrosis, and infiltrating cell inflammation in cerebellum. From examinations and observations it can be concluded that macroscopic and microscopic changes lead to the suspected Coryza (snot) disease.
Solasodine and Gosipol Effectivity as a Male Contraception Inhibit LH Expression and Spermatogenesis in Rat Wulansari, Desi; Oktanella, Yudit; Hendrawan, Viski Fitri; Agustina, Galuh Chandra
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.671 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.7

Abstract

Rabies related to increasing canine population in Bali. Uncontrolled wild animal populations caused disease transmission from animal to human. Various attempt at population control are carried out such as the use of natural contraception. Some compounds are known to have potential as antifertility are solasodine and gosipol. Solasodine is known have an antifertility affect. Gosipol, fenolic compound in Ceiba pentandra, inhibits spermatogenesis, reduce sperm concentration, motility and viability. This research aims to compare effectiveness of terong cepoka and Ceiba pentandra as antifertility. This research was conducted at Mei-November 2018. Eight-teen rats were used in this study and divided into three groups: control, P1 extract Solanum torvum 1g/kg BW and P2 extract Ceiba pentandra 0,1g/kg BW PO. Rats were treated with extract for 10 days and euthanazed at day 11. Testis were collected for histopathology using HE staining to observe spermatogenesis and using immunohistochemistry to observe LH expression. The result are analyzed using one way ANOVA P<0.05. the result show that extract solanum torvum 1g/kg BW and ceiba pentandra 0,1g/kgBW cannot reduce spermatogenesis and LH expression. This study used crude extract which still consist any other compound like antioxidant. Future study we need use isolated and pure solasodine and gosipol.
THE STUDY OF ORGANOPHOSPHATE (DIAZINON) TOXICITY TOWARD LIVER HISTOPATHOLOGY AND MALONDIALDEHYDE (MDA) SERUM LEVELS ON RATS (Rattus norvegicus) Pratama, Dyah Ayu Oktavianie; Aulia, Zulfa; Aulanni'am, Aulanni'am; Permata, Fajar Shodiq
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.95 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.3

Abstract

Diazinon is an insecticide that has a higher toxicity than other insecticides. Normally, insecticides are detoxified by liver, but this process produced free radicals which causes cell damage. Free radicals also increase lipid peroxidation which directly increase malondialdehyde levels. This research was aimed to determine the influence of diazinon toxicity to the liver histopathology and levels of malondialdehyde (MDA) in the serum of rats (Rattus norvegicus). The rats were divided into 4 groups which consist of negative control group and three experimental groups which were given diazinon 20 mg/kgBW (P1), 40 mg/ kgBW (P2), and 60 mg/ kgBW (P3). The rats were given diazinon for 8 weeks orally. The parameters used in this research was liver histopathology with hematoxilin eosin stain and Malondialdehyde levels with Thiobarbituric test. Liver histopathology was analyzed in descriptive qualitative and the level of MDA was analyzed quantitatively using ANOVA and Tukey's exact test with α = 0.05. The result of this research showed the influence of organophosphate (diazinon) on liver histolopathology was shown by inflammatory cells infiltration in hepatic parenchyma, sinusoidal congestion, and cytoplasmic vacuolation of the hepatocytes. Statistical analysis proved that the diazinon was able to increase the level of MDA serum significantly (p<0,05) up to 71% in experimental group P1 (dose 20 mg/kgBW), which the increase level of MDA serum depend on dose of diazinon. This research concluded that diazinon is one of the organophosphate pesticide that toxic based on liver histopathology and MDA serum levels. 
The Ointment of Peel Dragon Fruit (Hylicereus costaricensis) Extract Decreased the Interleukin-2 (IL-2) Expression and the Number of Mononuclear Inflammation Cells toward Open Wound in Skin of Rats Wistar Permata, Fajar Shodiq; Febrianto, Ahmad
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (926.321 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.4

Abstract

Open wounds healing is need more time because of the long inflammatory phase. Dragon fruit skin contains flavonoids and vitamin C which function as anti-inflammatory and cell regeneration. The purpose of this study was to determine the therapeutic effects of dragon fruit skin extract ointment (Hylicereus costaricensis) on the area of IL-2 expression and the number of mononuclear inflammatory cells in skin tissue in open wounds of rats (Rattus norvegicus) for 6 days of treatment. As many as 20 male rats, ± 180 grams, Wistar were divided into 5 groups namely negative control, positive control, ointment of dragon fruit skin extract with concentration of 5% (P1), concentration of 10% (P2) and concentration of 15% (P3). Wound formed as dorsal skin biopsy of dorsal 1x1 cm. Ointment was given topically 2x/day for 6 days. Rats were euthanized on day 7th and the skin tissues of the wound area were collected. The number of mononuclear inflammatory cells was counted in skin slides of HE staining and Expression Area expression of IL-2 was measured by ImmunoRatio® in immunohistochemical slides. Data were analyzed statistically using One Way ANOVA and Tukey Post Hoc Test. The results showed that dragon fruit skin extract ointment was able to reduce significantly (p<0.05) for area expression of IL-2 and the number of mononuclear inflammatory cells and the best concentration of ointment for treatment was 15%. This is due to the effect of flavonoids as anti-inflammatory and vitamin C as a trigger for cell regeneration. The conclusion of the study was that ointment of dragon fruit peel able to become a candidate for therapeutic medicine for open wounds.
Prevalence and Predisposing factors of Subclinical Mastitis in Dairy Cows in Bakir, Sukomulyo Village, Batu Regency Fatmawati, Mira; Setianingrum, Ani; Haskito, Ajeng Erika Prihastuti; Dameanti, Fidi Nur Aini Eka Puji
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (195.291 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.5

Abstract

Peradangan ambing pada sapi perah yang lebih dikenal sebagai mastitis masih menjadi masalah utama dalam industri susu. Kerugian peternak karena mastitis disebabkan oleh penurunan produksi susu dan penurunan kualitas susu yang dihasilkan. Pada umumnya pengetahuan peternak rakyat akan mastitis sebatas pada mastitis klinis karena gejala klinis yang dapat teramati. Mastitis yang sifatnya subklinis sulit dideteksi dan apabila dibiarkan tanpa penanganan dapat menjadi sumber penularan ke sapi perah lainnya. Deteksi mastitis subklinis pada sapi perah menggunakan uji tidak langsung yaitu california mastitis test (CMT) atau menggunakan uji langsung dengan penghitungan jumlah sel somatis (JSS). Penelitian ini merupakan studi lintas seksional dengan menggunakan sampel susu kwartir pada peternakan sapi perah rakyat di dusun Bakir, desa Sukomulyo, kabupaten Batu. Pengujian mastitis dilakukan di lapangan dengan uji CMT dan di laboratorium untuk penghitungan JSS. Pengambilan data dengan kuesioner untuk mengetahui faktor predisposisi mastitis. Prevalensi mastitis subklinis berdasarkan JSS adalah 60,22% dan berdasarkan uji CMT di lapangan adalah 18,20% dari total sampel kwartir. Faktor predisposisi yang mempengaruhi masih adanya kejadian mastitis pada peternakan antara lain kebersihan sapi, kandang, dan sanitasi sebelum dan sesudah pemerahan.
The Effect of Clove's and Lemongrass's Essential Oil in Basal Feed on Performances and Hematology Profile of Streptozotocin-Induced Diabetic Rat Baidlowi, Ahmad; Nesti, Dela Ria; Tjahajati, Ida
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.456 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.1

Abstract

This research was conducted to determine the effect of Clove’s and Lemongrass’s essential oil (EO) in basal feed on performances and hematology profile of Sterptozotocin-induced diabetic rat. 20 rats aged 40 days were divided into four groups of feed treatments, which are: negative control feed (basal feed-non-diabetic) (R1); positive control feed (commercial diabetic food) (R2); basal feed+Clove’s EO (R3); and basal feed+Lemongrass’s EO (R4). Each treatments using 5 rats. Collected data were performances and blood hematology profile. Data were analyzed using one way ANOVA design, the significance of different was tested using Least Significant Different (LSD) test. The result from this research showed that Clove’s and Lemongrass’s EO were significantly affected (P<0.05) on post-induction initial weight (5.41%; 3.30%; and 5.21%) compared to R2 group. It also significantly affected (P<0.05) on total gain (56.66% and 3.30%) and final weight (5.47% and 3.48%) compared to R1 group. Hematology profile’s data showed that both of Clove’s and Lemongrass’s EO did not affect to erythrocyte level, however, Clove’s EO did significantly affect to thrombocyte, hemoglobin and leukocyte level compared to R2 group, consecutively: (25.70%; 87.79%; and 37.59%), while Lemongrass’s EO significantly affected hemoglobin level (51.17%) compared to R2 group. It can be concluded that the addition of Clove’s and Lemongrass’s EO on basal feed could maintain the performance and improve the hematology profile of diabetic rat.
Classical Swine Fever di Papua, Indonesia: Demografi peternakan babi dan tingkat vaksinasi Nugroho, Widi
Veterinary Biomedical and Clinical Journal Vol. 1 No. 2 (2019)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.92 KB) | DOI: 10.21776/ub.VetBioClinJ.2019.001.02.6

Abstract

Classical Swine Fever(CSF) adalah penyakit penting pada industri babi. Pada akhir 2003, wabah CSF membunuh ribuan babi di Timika, Papua. Pengendalian CSF di Timika melalui program vaksinasi menggunakan strain C. Namun, demografi peternakan babi di Timika tidak diketahui sehingga tingkat vaksinasi tidak dapat dievaluasi. Penelitian bertujuan mengetahui demografi peternakan babi dan tingkat vaksinasi CSF di lima distrik diTimika.Studi dilakukan pada 25 dari 44 desa di lima distrik studi. Data demografi peternakan dikoleksi dilevel Rukun Tetangga dan dianalisis ditingkat desa. Data vaksinasi CSF per-desa dikoleksi selama 2018. Data demografi peternakan dan vaksinasi ditampilkan deskriptif, korelasi tingkat vaksinasi dengan jumlah babi, jumlah peternak atau ukuran peternakan ditingkat desa dianalisis pada level 0,05, menggunakan Excel 2010 (Microsoft, USA).      Hasil menunjukkan, di 25 desa studi, populasi babi 16.846 ekor, jumlah peternak 2.560 keluarga, penduduk 133.186 jiwa, jumlah babi rata-rata 6,6 ekor per-peternakan, rasio babi-manusia 0,13 ekor per-orang. Pada empat desa, vaksinasi menjangkau 70% populasi babi. Pada tujuh desa lainnya vaksinasi menjangkau kurang dari 30% populasi babi, namun 14 desa selebihnya tidak tervaksin. Tidak ada korelasi antara tingkat vaksinasi di satu desa dengan jumlah babi, jumlah peternak atau ukuran peternakan (P>0,05). Studi ini dapat menjadi referensi bagi desain pengendalian CSF di Timika dimasa mendatang.

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