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Veterinary Biomedical and Clinical Journal
Published by Universitas Brawijaya
ISSN : -     EISSN : 26221012     DOI : -
Core Subject : Health, Science, Education,
Biochemistry, Genetics & Molecular Biology Education Health Professions Immunology & microbiology Veterinary
Veterinary Clinical and Biomedical Journal (VBCJ) is periodically peer reviewed journal that publish every January and July per year since 2019. VBCJ accommodate any articles in the field of veterinary medicine, biomedical, as well as veterinary case report.
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Articles 64 Documents
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The Analysis of Pancreatic Duodenal Homeobox 1 (PDX-1) Gene Mutation Based on Blood Glucose Level on Domestic Cats (Felis catus) Post Ovaryohisterectomy Putri, Hana Mitsuki; Aulani'am, Aulani'am; Nurmaningdyah, Ajeng Aeka; Prasetyo, Dodik; Permata, Fajar Shodiq
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2021.003.02.5

Abstract

Ovariohysterectomy (OH) in domestic cats causes several side effects, including obesity. Obesity is closely related to diabetes. The glucoregulatory gene that will cause insulin resistance if there is a gene mutation is the PDX-1 gene. This insulin resistance will have a very immediate effect on blood glucose in the body. The study examined the relationship between PDX-1 gene polymorphisms and blood glucose levels in domestic cats with OH. The method used in this study was in vitro PCR amplification of the PDX-1 gene with Forward primers 'CCG AGC TGT CAA AGC TAG CG' and Reverse 'GAA CCG GTA GAG GGT CTT GG' and followed by sequencing of PCR products. The results showed fundamental differences in the base sequence in the PDX-1 gene sequences at c.435G>A, c.927A>T, and c.584A>G. The fundamental difference in the PDX1 gene sequence is a silent mutation
Effect of Jatropha(Jatropha curcas L.) Seeds Extract Induction on Liver Histopathology and SGPT levels (Serum Glutamic Piruvic Transaminase) on Rat (Rattus norvegicus) Fitria, Neny; Pratiwi, Herlina; Widodo, Edwin; Primajayanti, Angelia; Anggraini, Novia Dwi; Nurfitriani, Nurfitriani; Findi, Nadia
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2021.003.02.1

Abstract

Jatropha or Jatropha curcas L. is a plant of the Euphorbiaceae family with medicinal and non-medical uses, including oral contraceptives. The part of Jatropha that is often used is the seed. However, Jatropha seed also contains saponins, phorbol esters, currants, tannins which can cause organ poisoning. This study was conducted to determine the effect of Jatropha seed extract on liver histopathology and SGPT levels in Rattus Norvegicus rats, using a completely randomized design method. Experimental animals were divided into four groups, and each group consisted of five female rats. The groups were the control group without treatment and those treated with Jatropha seed extract orally at doses of 10, 50, and 100 mg/kg BW. The induction process was carried out every day for 14 days, on the 15th day, a necropsy was performed. Livers were taken for histological analysis, and blood samples were taken to measure SGPT levels. Results showed that the administration of Jatropha seed extract could increase SGPT levels. The administration of Jatropha seed extract at a dose of 100 mg/kg body weight induced the most severe damage of hepatocytes among the treatment groups.
Haemobartonellosis in a Domestic Cat in Indonesia: a Case Study Satriawan, Ivan; Octaviani, Devi Intan Dyah Ayu
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/10.21776/ub.VetBioClinJ.2021.003.02.3

Abstract

Haemobartonellosis is a disease caused by Haemobartonella felis or Mycoplasma haemofelis, which attacks red blood cells. Ctenocephalides felis could transmit Haemobartonellosis. Further, vertical transmission from mother to offspring and blood  transfusion from infected animals could transfer the disease to other animals. This report aimed to describe the diagnosis and medical treatment of haemobartonellosis in a domestic cat in Indonesia. Physical examinations and blood analysis were carried out. The cat weighed 1,4 kg with 39,3°C temperature, CRT was three seconds, mucosa looked pale, and many Ctenocephalides felis fleas were found. The clinical symptoms detected were weakness, loss of appetite, and hair loss. A blood test indicated normocytic normochromic anemia. The rod-like formation within erythrocytes in blood smear was observed. Infection with Hemobartonella felis was diagnosed based on the blood test and the presence of numerous Ctenocephalides felis. Doxycycline was administrated at 10 mg/kg BW once a day for 21 days. The cat recovered clinically after 21 days, with a good appetite, normal mucosal color, and normal temperature
The Effect of Administrated Infused Water of Red Dragon Fruit (Hylocereus polyrhizus (F.A.C. Weber) Britton & Rose) on Growth Performance of Broiler Chickens Susanto, Adi; Aditya, Nico Chandra; Damayanti, Sari'ah Cintami; Saragih, Hendry
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2021.003.02.4

Abstract

The use of synthetic Antibiotics Growth Promoters (AGP) in broiler rearing causes long-term negative effects. The use of red dragon fruit-infused water (IWBNM) as a natural supplement is necessary. Determine the effect of IWBNM on the growth of broiler chickens was the aim of this study. A completely randomized design using five groups with three replications was the research design used. Each replication consist of 20 chicken. IWBNM is administered through drinking water. The treatment was carried out for 21 days. The treatment consisted of P0 (Control) plain water: P (Treatment) concentration of dragon fruit. The treatment group is 1 = 0.25%; 2 = concentration 0.5% ; 3 = concentration 1% ; 4 = 2% concentration. The data was tested by One Way ANOVA, with the Least Significant Difference (LSD) test and Duncan as a follow-up test. The result showed that one ml of IWBNM contains a total flavonoid of 676,19 µg. Giving IWBNM for 21 days, in general, can improve the growth performance of broiler chickens. Measurement of morphometry and body weight on day 21 indicates the increasing growth performance of broiler chickens with the highest performance was achieved by the P4 group compared to P1, P2, P3, and P0. As for the ratio of visceral organs to body weight, IWBNM had no significant effect on both the heart and liver.
Review: Leptospirosis in Dogs in Indonesia Wiyata, Rifen Prabawan Krida Taruna; Nugroho, Widi
Veterinary Biomedical and Clinical Journal Vol. 3 No. 2 (2021)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2021.003.02.2

Abstract

Leptospirosis is an infectious disease caused by motile bacteria of the genus Leptospira. The prevalence of leptospirosis in dogs in Indonesia ranges from 13,8% - 44%, with Leptospira variants including the serovars Ichterohaemorragiae, Celledoni, Canicola, Pyrogenes, Cynopteri, Rachmati, Bataviae, Javanica, Grippotyphosa and Tarrasovi. Leptospira sp. can be transmitted through direct contact with an infected animal, soil or water contaminated with Leptospira sp. Rodents, pigs, horses, farm animals, dogs and various wild animals such as squirrels and deer can serve as carriers of Leptospira sp. Clinical symptoms of leptospirosis in dogs in Indonesia include fever, lethargy, anorexia, vomiting, jaundice, kidney disorders, dyspnea, polyuria, yellow urine, dehydration and death. The gold standard for leptospirosis testing in dogs is the MAT test, but the PCR and ELISA tests have higher sensitivity. Prevention of leptospirosis in dogs can be done by vaccination, routine disinfection of dog house and preventing dogs from direct contact with reservoir animals such as rats
Identification of Colibacillosis and Antibiotic Resistance Test in Free-range Chicken: Identifikasi Kolibasilosis dan Uji Resistensi Antibiotik pada Ayam Kampung Wulandari, Eka; Amri, Indah Amalia
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.4

Abstract

Escherichia coli are normal flora in the digestive tract of animals and humans but can be opportunistic under certain conditions. Apart from normal flora, there are also pathogenic strains of the E. coli bacteria that can cause disease in the hospes. The disease that can be caused by E. coli bacteria is colibacillosis which often occurs in poultry. One of the clinical symptoms that appear in chickens suffering from colibasillosis is diarrhea which can arise due to enteritis. This study aims to identify the incidence of colibacillosis and antibiotic resistance of E. coli bacteria in Free-range Chicken. Clinical symptoms showed that the animal looked weak, had diarrhea, and had feces around the cloaca. The necropsy results showed the presence of hemorrhage in the duodenum and caecum. Samples of organ swabs that experienced hemorrhage were then tested for isolation and identification of E.coli bacteria and antibiotic resistance using the Kirby-Bauer method for five types of antibiotics, penicillin, bacitracin, gentamicin, cefadroxil, and ciprofloxacin. The test results showed that the Free-range Chicken had colibacillosis. The E. coli bacteria that cause colibacillosis were resistant to penicillin and bacitracin antibiotics, intermediates to cefadroxil, and sensitive to ciprofloxacin and gentamicin.
Yolk Binding Treatment for Reticulated Python in House of Pet Clinic: Penanganan Kasus Yolk Binding Pada Ular Sanca batik (Python reticulatus) di Klinik House of Pet Isnaeni, Intan Kirana; Setianingrum, Ani; Hardian, Andreas Bandang; Yesica, Reza; Herlambang, Anugrah Niki
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.6

Abstract

Yolk binding is a condition where the egg yolk as food reserves while the snake still in the egg phase is notcompletely absorbed in the snake’s body when the snake has become neonatal. This study aims todetermine the handling of cases of yolk binding in Python reticulatus. A 3-month-old female Pythonreticulatus was brought by the owner to the vet with complaints of not defecating after ten days from thelast feeding on and the presence of a ¼ lump from the caudal. The results of the stool examinationshowed the number of protozoa. The x-ray results, show the air-filled medial with radiolucent colorintensity and a mass with radioopaque color intensity. Based on the signals, history, physical examination,and investigations, a diagnosis of yolk binding was obtained. The treatment given is surgery, drug therapy,and post-operative care. After being brought home, five days after surgery snake died.
Effect of Torbangun Leaf (Coleus ambonicus, L.) Extract Feeding on Cisplatin-Induced in Wistar Rats (Rattus norvegicus): Gambaran Histopatologi Ginjal yang Diinduksi Cisplatin dan Ekstrak Daun Torbangun (Coleus ambonicus, L.) pada Tikus Wistar (Rattus norvegicus) Solfaine, Rondius; Supriyanto, Melda Putri Elizabeth
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.2

Abstract

This study aimed to analyze the effect of torbangun leaf extract (Coleus ambonicus, L.) on kidney histopathology in cisplatin-induced Wistar rats. The design used was Completely Randomized Design (CRD) with three different treatments and six repetitions. The rats used were 18 male Wistar (Rattus norvegicus) rats, bodyweight 150-200 grams and 2-3 months of age. Treatment P0 for Wistar rats given aquadest ad libitum, P1 for Wistar rats injected with Cisplatin on day 4, intraperitoneally at a dose of 7 mg/kg with 1% of CMC NA suspension and P2 for Wistar rats given Torbangun leaf extract orally at a dose of 100 mg/kg BW for seven days. On day 4, groups P1 and P2 were injected with Cisplatin intraperitoneally at a 7 mg/kg BW. The non-parametric data from the study were statistically tested with the Kruskal Wallis test and the post hoc test in the form of the Mann-Whitney test. The test results from statistical analysis showed that the Torbangun leaf extract could reduce tissue damage by significantly reducing hemorrhage, congestion, and vasculitis in the treatment group
Identification and Morphometric Analysis of Toxocara cati in Domestic Cat at Ontosenovet Animal Clinic Malang: Identifikasi dan Analisis Morfometri Toxocara cati pada Kucing Domestik di Klinik Hewan Ontosenovet Malang Soegiarto, Ellen; Yesica, Reza; Antika, Dona Dwi
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.5

Abstract

Toxocariasis is a worm disease caused by infection of Toxocara cati. This study aims to identify the etiology, clinical symptoms, diagnosis, morphometry of eggs and worms in domestic cats at the Ontosenovet Animal Clinic Malang. The results of physical examination reveal enlargement of the abdomen, dull hair and hair loss. Hematological examination results showed a decrease in platelets, RDW-VC, and RDW-SD, an increase in lymphocytes, and MCHC. Examination of feces and worms and eggs is carried out at the Parasitology Laboratory of the Faculty of Veterinary Medicine, Universitas Brawijaya. Stool examination is carried out using native and floating methods which showed the presence of worm eggs in the second method. The eggs show a slightly elongated or oval shape, thick walls, and a mottled surface. Worm eggs have an average length of 71,61 μm and a width of 59,87 μm. Worms obtained from cat vomit were then made preparations with Acetocarmine staining. The results of worm observations show three lips on the anterior, posterior end of digitiform and curved to the ventral. The results of morphometric measurements of adult T. cati show that the diameter of the worm, the length of the posterior end of the worm, and the width of the lips obtained an average of 754,14 μm, 545,19 μm, and 314,87 μm respectively. Overall body lengths of worm samples 1, 2, and 3 are 7,50 cm, 8,70 cm, and 7,80 cm with an average of 8,00 cm. Based on the examination the cat had toxocariasis due to Toxocara cati. The therapy given is an anthelmintic Drontal® (¾ tablet) which contained 20 mg praziquantel and 80 mg pyrantel.
Case Study: Pyothorax in Domestic Short Hair Cat Hasanah, Nurmaulida; Mentari, Upakarti Dwi; Zaenab, Siti; Ichsanniyati, Zulfa
Veterinary Biomedical and Clinical Journal Vol. 4 No. 1 (2022)
Publisher : Faculty of Veterinary Medicine Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.VetBioClinJ.2022.004.01.1

Abstract

One of the diseases caused by pathogenic microbial infections is pyothorax. Pyothorax, also known as thoracic empyema, accumulates purulent exudates in the pleural space. This study aims to understand the diagnosis and therapy of pyothorax in Domestic Short Hair (DSH) cats. A male DSH cat came with a harsh breath condition or acute dyspnea. The cat was breathing more rapidly, having to make more effort to breathe. The anamnesis showed that the patient had a cough history and repeated with a dyspnea condition. Some diagnoses were radiographic examination (x-ray) and laboratory tests such as complete blood cell, blood chemistry, cytology, and bacterial culture. Result of radiography examination and laboratory examination showed that increasing of white blood cells or leukocytosis in complete blood cell test, fluid loss (dehydration) in the blood chemistry test, radiography (x-ray) imaging saw the abnormalities in the lungs that was a white color (radio-opaque) and looked homogeneous. Thoracocentesis founded pleural effusions in the form of pus/pyo, cytology examination found polymorphonuclear cells (PMN) neutrophils and mononuclear cells (MN), and the result of pus bacterial culture was Acinobacter baumannii. The examination results in patients showed positive pyothorax with causal of Acinobacter baumanii. Antibiotic therapy sensitive to Acinobacter baumanii had been proven effective until the patient recovered. The antibiotics for therapy started with 10 mg/kg BW of Enrofloxacin, 20 mg/kg BW of Meropenem, and 20 mg/kg BW of Tigecycline. Tigecycline has proven to be effective until the cat recovers.

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