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Anna Safitri
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Research Center for Smart Molecule of Natural Genetics Resources (SMONAGENES) office: 2nd floor MIPA Building, Faculty of Mathematics and Natural Sciences, Universitas Brawijaya, Jl. Veteran Malang, East Java, Indonesia – 65145
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JSMARTech : Journal of Smart Bioprospecting and Technology
Published by Universitas Brawijaya
ISSN : 26860805     EISSN : 27147894     DOI : https://doi.org/10.21776/ub.jsmartech.2020.001.02.
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience
JSMARTech : Journal of Smart Bioprospecting and Technology (p-ISSN: 2686-0805, e-ISSN : 2714-7894) is an Open Access Scientific Journal published by Research Center of Smart Molecule and Natural Genetics Resources (SMONAGENES), Universitas Brawijaya, Malang, East Java, Indonesia, since 2019. It is a journal covering of bioprospecting, biochemical, biotechnology, bioinformatics, natural product, pharmaceuticals, biomedical, genetics engineering, nutrigenomic, and nanotechnology. The journal publishes a manuscript written in English for original research papers, short communications, and review articles. The paper published in this journal implies that the work described has not been, and will not be published elsewhere, except in abstract, as part of a lecture, review or academic thesis.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 77 Documents
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Front Matter JSMartech Vol.04., No.01 Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 1 (2023): JSMARTech Volume 4, No. 1, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.01.01

Abstract

Virtual Prediction of Phytosterol Compounds from Pegagan (Centella asiatica L.) As Inhibitor for Interleukin-6 (IL-6) to Prevent Rheumatoid Arthritis Disease Azis Mawardi; Rossa Martha Wulandari; Moch Mustakim; Nurul Jadid Mubarakati
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.72

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease in which the joint area is inflamed which results in swelling, pain and often causes damage to the inside of the joint. Rheumatoid Arthritis is characterized by decreased Interleukin-6 (IL-6), so that Interleukin-6 (IL-6) inhibitors are needed. This strategy is convincing for controlling RA. Pegagan have to play role in overcoming the problem of joint inflammation. Phytosterols are compounds that are commonly found in pegagan and include antioxidants that are beneficial for health. This study aims to determine the mechanism pathway of pegagan's active compounds in the treatment of RA. This study used an in silico approach by analyzing target proteins and active compounds of pegagan through Lipinski rule of five and molecular docking analysis. This study used 3 phytosterol compounds including campesterol. β-sitosterol. and stigmasterol. The control used in this study was the tartaric acid compound whose receptor molecule was interleukin-6 (IL-6) with PDB ID: 1-ALU. The results of the Lipinski rule analysis found that all the test compounds used met the criteria and could be applied as drugs. Based on the results of molecular docking, stigmasterol has a good binding affinity value and is able to exceed the control compound with the highest binding affinity with score of -6.4 kcal/mol. Visualization results show that there is an interaction formed between the active compound and the target protein so that phytosterl compounds have a role that can be an alternative medicine in the prevention and treatment of RA.
Antimicrobial Activity of Tobacco Flower Extract (Nicotiana tabacum L.) in Various Solvent Lina Oktavia Rahayu; Ernanin Dyah Wijayanti; Fajriana Ikra Pratidina; Vanny Sasmita Ayuningtyas
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.67

Abstract

Tobacco plants (Nicotiana tabacum L.) are known to contain secondary metabolites that function as antimicrobials. But thus far only the leaves, roots, and stems have been utilized. The research aimed to determine antimicrobial activity of tobacco flower extract in various solvent. The tobacco flowers were extracted used ethanol, methanol, and N-Hexane solvents. The extracts were tested for antimicrobial activity against Staphylococcus aureus and Candida albicans by dilution method. The results showed that ethanol and methanol extracts of tobacco flowers had a Minimum Inhibitory Concentration (MIC) value of 32% and a Minimum Bactericidal Concentration (MBC) of 64% against S. aureus and C. albicans. As for the N-Hexane extract of tobacco flowers, it has MIC value of 64% and MBC of 64% against C. albicans. In conclusion, tobacco flower extract in ethanol, methanol, and N-hexane solvent have potential functions as antimicrobial agents.
Front Matter JSMartech Vol.04., No.02 Fatchiyah Fatchiyah
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 4 No. 2 (2023): JSMARTech Volume 4, No. 2, 2023
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2023.004.02.38

Abstract

Phytochemical Profile and Antioxidant Potential of Tobacco Flower Extract in Different Solvents Wijayanti, Ernanin Dyah; Rahayu, Lina Oktavia; Dzulaikha, Shovia; Ayunda, Fiddini Robetta; Djara, Selfiana
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.04

Abstract

Tobacco (Nicotiana tabacum) is a commercial plant that is extensively used, particularly for its leaves. Thus far, exploration on the flower part is still limited. The objective of this study was to evaluate the phytochemical profile and antioxidant activity of tobacco flower extracts in different solvents. Tobacco flowers were macerated separately in methanol, ethanol, and n-hexane. Each extract was assayed by color reaction test to detect the phytochemical content. Total phenolic content (TPC) and antioxidant activity were determined by Folin-Ciocalteu and DPPH methods, respectively. Flavonoids, saponins, alkaloids, and triterpenoids are detected in methanol and ethanol extract, anthraquinones is only detected in methanol, while glycosides and steroids are only detected in n-hexane extracts. Among the other extracts, the methanol extract was the richest in phytochemical compounds and had the highest TPC (132.589±0.662 mgGAE/g). However, tobacco flower extract in all solvents were less effective as antioxidant due to very high IC50 values (>500 µg/mL).
Investigation of Total Phenol Content in Red Pomegranate Leaves Extract (Punica granatum L.) Januarista, Terra; Mubarakati, Nurul Jadid; Ramadhan, Majida
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.09

Abstract

Red pomegranate leaves (Punica granatum L.) is one of the plants that has many benefits and potential as herbal medicine. Red pomegranate leaves have bioactivities such as alkaloids, flavonoids, phenols, saponins, tannins and terpenoids so that they can be used as an alternative antioxidant. This study aims to determine the comparison of the amount of total phenol content in red pomegranate leaves extract using maceration and digestion extraction methods. Total phenol content was determined using the Folin Ciocalteau method with a spectrophotometer UV-Vis. The research method carried out was descriptive experimental quantitative and data analysis was carried out using one way ANOVA. The results of the determination of total phenol content in gallic acid obtained a regression equation y = 0.012x + 0.545 with R2 = 0.9981. The results showed that the total phenol content in the ethanol extract of red pomegranate leaves was greater with the maceration method of 91.83 (µg GAE/g sample) compared to the digestion method of 83.27 (µg GAE/g sample). It can be concluded that the two methods used on red pomegranate leaf samples have no significant difference in total phenol content.
Comparison of Antioxidant Activity of Mistletoe Mango Leaves Extract (Dendrophtoe pentandra (L.) Miq) Using Ethanol Solution and Methanol Solution Based on DPPH Method Silvia, Putri Azizatul; Sjakoer, Nour Athiroh Abdoes; Mubarakati, Nurul Jadid
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.15

Abstract

Indonesians have been utilising plants as traditional raw materials, especially herbal plants, since the time of the struggle until the current independence. Mistletoe mango which belongs to the Loranthaceae family, leaves of mistletoe mango is considered a medicinal plant. Phytochemical analysis shows that these plants contain a number of secondary metabolites, such as flavonoids, saponins, tannins, alkaloids, glycosides, and inulin. Antioxidants are compounds that can prevent oxidation reactions by binding free radicals and highly reactive molecules. The DPPH method is a simple, easy, rapid and sensitive antioxidant activity test method that uses only a small sample. The aim was to determine the comparison of the antioxidant activity of mistletoe mango leaves extract (Dendrophthoe pentandra (L) Miq) with ethanol and methanol solvents, as well as analyzing the 50% inhibitin of DPPH. The results showed that the antioxidant activity of mistletoe mango leaves extract using ethanol solvent was 15,286 ppm, while using methanol solvent was 93,456 ppm. It was concluded that antioxidant activity showed a very strong category and there was a significant difference.
Development of Bacillus subtilis Detection Method Targeting Genes codY, narH, and ureC Using Polymerase Chain Reaction Nurjayadi, Muktiningsih; Berkahingrum, Ayu; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Anggraeni, Rosita Gio; Fahriza, Tiara; Declan, Jefferson Lynford; Putri, Gladys Indira; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El Enshasy, Hesham
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.26

Abstract

Bacillus subtilis, a causative agent of foodborne illness in bread, cakes, cereals, and cheese leading to symptoms like diarrhea and nausea, was investigated for its role in nosocomial infections, including endocarditis, bacteremia, septicemia, and meningitis. The research focused on three genes: codY, narH, and ureC, which respectively contribute to bacterial survival through biofilm and spore formation, acid resistance, and adaptation to anaerobic conditions. The objective of this study was to evaluate the codY, narH, and ureC genes using the Gradient PCR method for Bacillus subtilis detection in bread and cheese through Real-Time PCR. During the research, bacterial growth was observed with an OD600 of 1.438 in Tryptic Soy Broth (TSB), and colonies of medium size, smooth, cream-coloured, and round shape were successfully isolated on Tryptic Soy Agar (TSA). The DNA template used had a 71.5 ng/µL concentration with an A260/280 ratio of approximately 1.830. The annealing temperature for Gradient PCR used was 53-62°C. The primers successfully amplified codY (175 bp), narH (222 bp), and ureC (153 bp) gene amplicons. The optimal annealing temperature for the primers used was 60°C, as indicated by the presence of a single bright band in electrophoresis. Using these three different genes, testing can also be conducted with the Multiplex PCR method. The next step involves developing a detection kit using optimized primers and annealing temperatures for the identification of Bacillus subtilis in bread and cheese samples using Real-Time PCR.
Diastase Enzyme Activity and Antibacterial Effectiveness to Gastroenteretis by Apis mellifera Honey from Indonesia Rosyidah, Rara Annisaur; Hasan, Akhmad Endang Zainal; Andrianto, Dimas
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.20

Abstract

The quality of monoflora honey (Apis mellifera) as an antibacterial can be influenced by the activity of the diastase enzyme and the secondary metabolites of the nectar source. This study aims to compare the activity of the diastase enzyme and the effectiveness of monoflora honey derived from Apis mellifera bees as a natural antibacterial agent against Salmonella typhi. The sampling method for monoflora honey from nine different nectar sources (acacia, calliandra, rambutan, eucalyptus, randu, mango, robusta coffee 1, robusta coffee 2, and rubber). The results showed that the highest diastase enzyme activity in monoflora honey was mango, rambutan, and robusta coffee 2 at 20.00 DN, eucalyptus, randu, and rubber at 10.00 DN, acacia at 6.67 DN, robusta coffee 1 5.00 DN, and calliandra 4.00 DN. Zone of inhibition for S. typhi on robusta coffee 2, robusta coffee 1, randu, eucalyptus, acacia, mango, rubber, rambutan, and calliandra respectively at 18.53; 16.70; 15.27; 14.70; 13.20; 11.20; 11.17; 9.47; and 6,80. The minimum concentration inhibition was 8 μg/mL.
Effects of Ovariectomy on Kidney Injury in SDT Fatty Rats Watanabe, Kana; Saigo, Yasuka; Sasase, Tomohiko; Uno, Kinuko; Miyajima, Katsuhiro; Ohta, Takeshi; Maekawa, Tatsuya
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 2 (2024): JSMARTech Volume 5, No. 2, 2024
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Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.02.37

Abstract

Postmenopausal women are at a higher risk of developing diabetes, and estrogen can lower blood glucose levels. The purpose of this study was to investigate the effect of ovariectomy (OVX) on the pathology of diabetic nephropathy. Female SD rats and SDT fatty rat underwent bilateral OVX or sham treatment at 6 weeks of age, and their blood and kidneys were collected at 40 weeks of age for blood biochemical and histopathological analyses. OVX treatment resulted in a decrease in blood estradiol levels at 24 and 40 weeks of age. Histopathological analysis revealed that Sham-treated SDT fatty rats showed glomerular atrophy, adhesions, enlargement, mesangial cell proliferation, regenerated tubules, hyaline casts, Armani-Ebstein lesions, tubular dilatation, interstitial inflammatory cell infiltration, and fibrosis. OVX treatment slightly reduced glomerular atrophy and enlargement, renal tubular dilatation, and interstitial inflammatory cell infiltration, while slightly increased or exacerbated mesangial cell hyperplasia, tubular regeneration, and Armani-Ebstein lesions. The above findings suggest that SDT fatty rats are not a practical model to study postmenopausal diabetic nephropathy.

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