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Medicra (Journal of Medical Laboratory Science/Technology)
Published by Universitas Muhammadiyah Sidoarjo
ISSN : 25807730     EISSN : 25807730     DOI : https://doi.org/10.21070/medicra
Core Subject : Health,
Health Professions Public Health
Focus : to facilitate scholar, researchers, and lecturers for publishing the original articles of review articles. Scope : Medicra publishes research articles in the field of “medical laboratory (science/technology)” with the following scope: Clinic Chemical Hematology Microbiology Parasitology Immunology Food and beverage analysis Chemical Molecular Diagnostics Toxicology Cytology Histology Epidemiology Laboratory Management Laboratory Quality Control
Arjuna Subject : Profesi Kesehatan - Teknologi Laboratorium Medis
Articles 129 Documents
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The Effect Of Centrifugation Speed On The Results Of Urine Sediment Examination: Hubungan Pengaruh Kecepatan Sentrifugasi Terhadap Hasil Pemeriksaan Sedimen Urine Hasanah, Muslimah Uswatun; Puspitasari, Puspitasari
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 5 No. 2 (2022): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i2.1668

Abstract

The process of centrifugation in urine examination is very important because it can be used in the manufacture of urine sediment. The purpose of this study was to determine the effect of centrifugation speed on the results of urine sediment examination. Centrifugation speeds used were 3000 rpm, 3500 rpm, 4000 rpm, and 4500 rpm using pathological urine samples. The samples used were 32. The research method used was an experimental laboratory at the Pusura Yos Sudarso clinical laboratory in Surabaya. The results of the study with a speed of 3000 rpm obtained an average of erythrocytes = 6.63/LPB, leukocytes = 13.63/LPB, and epithelial = 10.50/LPB. At a speed of 3500 rpm the average value of erythrocytes = 7.63/LPB, leukocytes = 15.13/LPB, and epithelium 11.63/PLB. At a speed of 4000 rpm the average value of erythrocytes = 8.63/LPB, leukocytes = 17.75/LPB and epithelial = 13.50/LPB. At a speed of 4500 rpm the average value of erythrocytes = 9.63/LPB, leukocytes = 20.50/LPB, and epithelial = 15.38/LPB. The results of the statistical test One Way Anova obtained p-value results of 0.001 < (0.05) in erythrocyte cells, p-value results for leukocytes 0.025 < (0.05), and p-value results for epithelial cells 0.002 < (0.05) means that there is an effect on the centrifugation speed on the results of urine sediment examination.
Relationship of Leukocyte Examination to Intra Uterine Growth Restriction (IUGR) in Pregnant Women Experiencing Anemia: Hubungan Pemeriksaan Leukosit Terhadap Intra Uterine Growth Restriction (IUGR) Pada Ibu Hamil Mengalami Anemia Santoso, Andreas Putro Ragil; Masruroh, Nur; Filia, Sisca
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 5 No. 2 (2022): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v5i2.1687

Abstract

Anemia in pregnant women will often occur because of increasing gestational age, the increasing need for nutrients in the form of iron in the body which increases during the 2nd to 3rd trimester of pregnancy. Anemia has an impact of around 14-62% on pregnant women including the occurrence of Intra Uterine Growth Restriction (IUGR) which is a condition that causes fetal growth to be stunted which is characterized by the size and weight of the baby that is not appropriate during pregnancy. The next thing to worry about is the emergence of infection if the patient has IUGR. The purpose of this study was to analyze the relationship of leukocytes to IUGR in pregnant women who are anemic with the parameters measured being leukocyte levels, IUGR values in anemic patients. The study was carried out in an analytic observational manner, then samples were obtained from 24 patients. Then, the patients filled out informed consent indicating that the patient was willing to be a research sample. Furthermore, the patient's blood was taken to measure leukocyte levels and based on the KIA book, IUGR levels were measured. Based on the above, it shows that the value of the results of the Spearman correlation test is 0.085 (p> 0.05). It can be concluded that there is no relationship between leukocyte levels and IUGR values in anemic patients at IBI Surabaya Hospital.
The Effect Of Delay Time And Storage Temperature On The Results Of Urine Leukocyte Examination In Patients Of Urinary Tract Infection (UTI): Pengaruh Lama Waktu Penundaan Dan Suhu Penyimpanan Terhadap Hasil Pemeriksaan Leukosit Urine Pada Pasien Infeksi Saluran Kemih (ISK) Krisdianti, Rika; Puspitasari, Puspitasari; Aliviameita, Andika
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1660

Abstract

Urinary Tract Infection (UTI) is a condition where there are microorganisms that multiply in the urinary tract causing inflammation and bacteriuria. The delay in the examination results in an error in diagnosis, the examination must be carried out >1 hour and a maximum of 2 hours after the voiding process at a temperature of 20-25℃ and the delay can be done a maximum of 4 hours at a temperature of 2-8℃. The study was conducted in June 2022. The study was conducted at SMK Muhammadiyah 1 Pandaan. UTI sampling at Sahabat Hospital in Suwayuwo Sukorejo. The purpose of this study was to determine the effect of delay time and storage temperature on the results of urine leukocyte examination in UTI patients. This study used an experimental method using 35 urine samples. The statistical test used the Two Way Anova test with a 95% confidence level and the inaccuracy limit of (α) = 0.05. Based on the results of the Two Way Anova test between storage temperature and delay in urine leukocytes, a significance value of 0.981 (p>0.05), it can be concluded that Ha is rejected and Ho is accepted, meaning that there is no interaction effect between the length of time delay and storage temperature on leukocyte results. urine in UTI patients.
Test The Effectiveness Of Japanese Papaya Leaf Extract (Cnidoscolus aconitifolius) On Aedes aegypti Larvae Mortality : Uji Efektivitas Ekstrak Daun Pepaya Jepang (Cnidoscolus aconitifolius) Terhadap Mortalitas Larva Aedes aegypti Ardiansyah, Syahrul; Nafsi, Faizatun; Hanum, Galuh Ratmana
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1671

Abstract

Dengue hemorrhagic fever (DHF) is caused by the dengue virus which is transmitted through the bite of the Aedes aegypti mosquito. Prevention of DHF can be done by using larvicides. Synthetic chemical larvicides are considered to have side effects in long-term use and can cause environmental pollution. So we need an alternative larvicides with natural ingredients from plants, namely Japanese Papaya plants. This study aims to determine the effectiveness of Japanese Papaya (Cnidoscolus aconitifolius) leaf extract on the mortality of Aedes aegypti larvae. This type of research uses an experimental study design. This study used 6 treatments with 4 repetitions consisting of control without treatment, P1 (0.2%), P2 (0.4%), P3 (0.6%), P4 (0.8%), and P5 (1%). Observations were made for 48 hours. From the research results obtained LC50 of 1.095%. The results of statistical tests using One-Way ANOVA showed that there was a significant effect on the mortality of mosquito larvae.
Prevalence Of Helmint Infection In Cat’s Feces In Surabaya City: Prevalensi Infeksi Helmin Pada Feses Kucing Di Kota Surabaya Rohmayani, Vella; Arimurti, Anindita Riesti Retno; Romadhon, Nurhidayatullah; Lihabi, Lihabi
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1685

Abstract

Cats are one of the most popular animals because of their cute and tame appearance. Just like other organism, cats can become infected worms if they are not kept clean. Cats infected with worms will initially lose their appetite, over time they will lose weight. Cats infected with worms, if kept and not treated continuously, can transmit to cat owners (humans). This study aims to determine the prevalence of parasitic worm infection in cat feces in the city of Surabaya, and to compare the prevalence of parasitic worm infection in domesticated cats with cats living wild in the city of Surabaya. The samples used in this study were 100 faecal samples, which were taken from 50 cats that live in the wild and from 50 live cats that are kept. Examination of faecal samples was carried out directly and indirectly using the saturated NaCl debris method, the data obtained will be presented descriptively and analyzed using the Chi-square test. Based on the results of the research conducted, the prevalence of worm infection in feral cat feces was 60%, while in domestic cats the prevalence was 10%. The types of worms that were found were Toxocara spp., Ancylostoma spp., Diphyllobothrium spp., and Trichuris spp. Based on the Chi-square test, it can be concluded that there is a significant relationship (P<0.05) between the prevalence of parasitic worm infection in domesticated cats and cats that live in the wild.
Perineal Ectopic Testis in One and A Half Year Old Child As A Rare Congenital Anomaly: A Case Report: Ektopik Testis Perineal pada Anak Satu Setengah Tahun Sebagai Kasus Kelainan Kongenital Yang Langka: Laporan Kasus Surya, Prima Ardiansah; Faadilah, Ashifa Hasna; Sriyono
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1689

Abstract

Ectopic testis is described as an abnormality of testicle development in which one or both testicles aren’t descending through the testicle normal pathways. Among all newborns with undescended testis, only 1% are diagnosed as ectopic testis. In this case, an 18 months old boy was presented to hospital with chief complaint of impalpable testis dextra. Phsical examination showed the right testis was not palpable in the scrotum and perineum. Ultrasonography examination showed a right testis in the perineum with normal volume and vascularization. Right orchidopexy was performed to correct the location of the testis. Early diagnostic of ectopic testis must be done through anamnesis, physical examination, and advanced examination. Limited faciliiesy and lack of parental knowledge make the patients come late to health facility. The conclusion of this study is knowledge among parents and skills of health workers concerning congenital anomaly especially undescensus testis should be improved.
Analysis Of The Inhibitory Ability Of Spike Attachment Of The Delta Variant Of Sars Cov-2 With Ace2 By The Active Compound In Turmeric (Curcuma longa L.) In Silico : Analisis Kemampuan Penghambatan Penempelan Spike Sars Cov-2 Varian Delta Dengan Ace2 Oleh Senyawa Aktif Pada Kunyit (Curcuma longa L.) Secara In Silico Liyaajul, Pratasyah; Mushlih, Miftahul; Rini, Chylen Setiyo; Rohmah, Jamilatur
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1703

Abstract

Turmeric (Curcuma longa L.) is an herbal plant that has many benefits as a treatment, including during the COVID-19 pandemic, one of the mechanisms of inhibition of SARS CoV-2 is to inhibit the attachment of ACE2 with Spike. The binding of the spike protein to the ACE2 receptor will produce conformational changes in the S protein, this study was conducted using an in silico method (computational analysis) which aims to determine the potential efficacy of Turmeric and its effectiveness in inhibiting the Delta variant of SARS CoV-2. The active compound contained in Turmeric (Curcuma longa L. ) obtained from the KNApSAcK database To determine compounds that can have potential and have good effectiveness in inhibition of the Delta Variant of SARS CoV-2, an analysis was carried out by looking at the binding energy and conformation changes that occur at the sticky point in each compound. Three-dimensional structure of SARS CoV-2 Varian Delta downloaded from the Protein Data Bank with PDB code 7V8B. Based on the analysis carried out, it was found that the compound (E)-nuciferoll has the lowest binding energy value of -1212.59 kcal / mol and is located at the initial attachment but cannot change the conformation, but from the sticky point of the compound (E)-nuciferol lies in the initial attachment of RBD-ACE2.
Comparison of HCG Levels in Reagents Temperature 25°C and 8°C Using ELISA Method : Perbandingan Kadar HCG Pada Reagen Suhu 25°C dan 8°C Menggunakan Metode ELISA Telleng, Prisca Audra; Santosa, Budi; Kartika, Aprilia Indra
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1709

Abstract

HCG hormone is a hormone produced by the placenta in early pregnancy with the aim of maintaining pregnancy. The Elisa method is a method for examining HCG levels which has the principle of specific antigen and antibody reactions. The work stage in the laboratory that has the biggest error is in the pre-analytic stage reaching 60%, one of which is the reagent temperature. The ELISA method recommends the reagent temperature to be stored at 2-8°C and when used at 25°C. Examination reagents Elisa method uses reagents that use enzymes in the examination. Enzymes are very sensitive to temperature. This study aims to determine the ratio of HCG levels at reagent temperatures of 25°C & 8° using the ELISA method. This type of research is analytic supported by experiments and literature studies. Serum samples of 6 pregnant women were taken from the Kedungmundu Public Health Center using a limited population count formula. The examination was carried out by comparing the reagents at a temperature of 25°C and 8°C using 6 serums of pregnant women and in duplicate. Data analysis only used primary data and statistical tests were carried out using normality, homogeneity and T-Test tests. The average result at a temperature of 25°C was 380.851 and the average temperature of 8°C was 321.99 and the T-test statistic test showed p value = 0.469 and 0.470. Based on the results of the study, it can be concluded that there is an insignificant difference in the concentration of HCG by comparing the reagents at 25°C and 8°C.
Potential of Seed Powder (Tamarindus indica L.) on Cholesterol Levels: Potensi Seduhan Serbuk Biji Asam Jawa (Tamarindus indica) Terhadap Kadar Kolesterol Purwaningsih, Nur Vita; Widyastuti, Rahma; Maulidiyanti, Ellies Tunjung Sari; Saputro, Tri Ade; Rohmayani, Vella; Ainutajriani, Ainutajriani
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1713

Abstract

ABSTRACT Coronary heart disease (CHD) is the leading cause of death after stroke in all age groups at 12.9%. The main reason is the amount of cholesterol in the blood. Tamarind (Tamarindus indica) is a traditional herb that has the potential to lower cholesterol, because it contains flavonoids, saponins, foliphenols, pectin, organic acids, vitamin B and vitamin C that can lower cholesterol. The purpose of this study was to determine the effect of tamarind seed powder (Tamarindus indica) on cholesterol levels. This type of research is experimental with pre-test and post-test with control group research design. The population and sample of this study were a group of mice totaling 32 mice. The location of this research was conducted at the Animal Husbandry Center (PUSVETMA). Data collection procedures were carried out by examining the cholesterol levels of mice before and after treatment. The data obtained were followed up with normality test and independent t test. The results of the study using the unpaired t test gave the result of p=0.000 (p<0.05). The conclusion of this study is that there is an effect of seed powder (Tamarindus indica) on cholesterol levels. Keyword : Seeds Tamarindus indica L., cholesterol levels, Mice (Mus musculus)
Diagnostic Test of FNAB Towards Histopathology in Lipoma Soft Tissue Tumor Patients: Uji Diagnosis FNAB Terhadap Histopatologi Pada Pasien Tumor Jaringan Lunak Lipoma Jihan Azzahra Arsyi; Handayati, Anik; Sri Sulami Endah Astuti
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 2 (2023): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i2.1653

Abstract

Lipomas are the most common soft tissue tumor neoplasms. Their range from benign lipomas to high grade liposarcomas. Liposarcoma happens when the neoplasm of a lipoma is malignant. There are various diagnostic methods that can be used to evaluate lipomas, one of which is Fine Needle Aspiration Biopsy (FNAB). FNAB is the initial diagnosis to determine the diagnosis of lipoma soft tissue tumors. Histopathology is the definitive diagnosis or the gold standard for diagnosing lipoma soft tissue tumors. This research aims to determine the value of the Fine Needle Aspiration Biopsy diagnostic test against histopathological tests as the gold standard in lipoma soft tissue tumor patients. This research is a retrospective with a descriptive observational design. This research involved taking medical records of patients with lipoma soft tissue tumors who were examined by FNAB at RSPAL dr. Ramelan, Surabaya for the period August 2019 - December 2021. The results of the FNAB examination were then matched against the histopathological examination data as the gold standard. Data processing using 2x2 cross tabulation to calculate the diagnostic test which includes sensitivity, specificity, positive predictive value, negative predictive value, and accuracy against the gold standard. The results of the Fine Needle Aspiration Biopsy diagnostic test for histopathology in soft tissue lipoma tumors obtained sensitivity of 100.00%, specificity 95.83%, positive predictive value (NRP) 87.5%, negative predictive value (NRN) 100.00%, and 96.77% accuracy. Fine Needle Aspiration Biopsy (FNAB) has good diagnostic value for diagnosing lipoma soft tissue tumors.

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