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INDONESIA
HAYATI Journal of Biosciences
Published by Institut Pertanian Bogor
ISSN : 19783019     EISSN : 20864094     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
HAYATI Journal of Biosciences (HAYATI J Biosci) publishes articles and short communication in tropical bioscience fields such as development, biotechnology, biodiversity and environmental issues. HAYATI J Biosci covers wide range of all life forms topics including virus, microbes, fungi, plants, animal and human. HAYATI J Biosci has been also indexed/registered in Crossref, DOAJ, CABI, EBSCO, Agricola and ProQuest.
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Articles 25 Documents
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Search results for , issue "Vol. 32 No. 2 (2025): March 2025" : 25 Documents clear
Efficiency of Bacillus pseudomycoides RAY21 and Bacillus subtilis CYA27 Endospore Formulation on Biochar and Oil Spill Dispersant Rahmatullah, Rifki; Anwar, Saiful; Yani, Mohamad; Firlandiana, Maulidi
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.374-386

Abstract

Bacillus sp. is well known for its functional capabilities such as solubilizing phosphorus (P) and potassium (K), and fixing nitrogen (N2). These bacteria can form endospores under stressed conditions, allowing long-term survival and application in biotechnological fields. This study aims to isolate Bacillus sp. capable of forming endospores and evaluates their viability on different carriers, specifically biochar and oil spill dispersant (OSD), to enhance biodegradation in contaminated environments. Soil samples from the bamboo rhizosphere were heat-shocked to isolate endospore-forming Bacillus strains, with the isolate identified as Bacillus pseudomycoides RAY21 through 16S rRNA sequencing. This strain exhibited Gram-positive characteristics, formed endospores, and demonstrated potential on various media such as Pikovskaya, Alexandrov, and N-Free Mannitol. The physiological characterization indicated optimal growth in a pH range of 6-8, salinity up to 3.5%, and thermophilic properties. Endospores from B. pseudomycoides RAY21 and B. subtilis CYA27 were tested on biochar and OSD as carriers. The results showed that endospores adhered better to biochar, but their viability was more stable in OSD over time. Notably, B. pseudomycoides RAY21 on OSD degraded 23.43% of total petroleum hydrocarbons (TPH), outperforming B. subtilis CYA27 (21.62%). In conclusion, the study demonstrates the potential of using Bacillus endospores on OSD as an effective carrier for bioremediation, particularly in degrading petroleum hydrocarbons. Future research should focus on optimizing carrier materials and exploring field-scale applications for enhanced environmental cleanup.
Genetic Population of Hypothenemus hampei Ferarri (Coleoptera: Scolytinae) from Coffee (Coffea spp.) in Sumatra, Indonesia Using The Cytochrome Oxidase Subunit I Gene Sitompul, Aida Fitriani; Dahelmi, Dahelmi; Roesma, Dewi Imelda
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.472-483

Abstract

Hypothenemus hampei Ferrari, 1867, is a globally significant pest of coffee (Coffea spp.). Genetic information about H. hampei from various locations, including countries in America, Africa, and Asia (Java, Indonesia), is available. However, the data has yet to be available for Sumatra. This research aims to study the genetic population of H. hampei in coffee plants in Sumatra. In this study, a total of 27 mitochondrial cytochrome oxidase subunit 1 (COI) gene sequences were used to estimate the population genetics of H. hampei in Sumatra, collected from C. arabica, C. canephora, and C. liberica at nine locations. The analysis of the COI gene sequences revealed that they contained 236 base pairs (53.76%) of conserved sites, 203 base pairs (46.24%) of variable sites, 153 base pairs (34.85%) of parsimony sites, and 50 base pairs (11.38%) of informative single sites out of a total of 439 base pairs. Haplotype analysis of the COI gene in H. hampei from Sumatra revealed 10 haplotypes, with a haplotype diversity (h) of 0.649 and nucleotide diversity (π) of 0.004. Genetic differentiation (Fst) of H. hampei is low among populations in Sumatra. Genetic variation within populations is higher, and between populations is low. The genetic distance of 0-0.28%, 27 H. hampei sequences from Sumatra are in the same branch, indicating low genetic variation. This information holds great potential for designing sustainable control strategies to manage this pest species in coffee plants, particularly in the Sumatra region.
Cytotoxicity of Ethyl Acetate Extract of Avocado Seeds (Persea americana Mill.) on Mouse Melanoma B16F10 Cell Line Sebayang, Shalina; Mariya, Silmi; Rayendra, Raendi; Wientarsih, Ietje; Priosoeryanto, Bambang Pontjo
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.405-413

Abstract

Artificial whitening agents available in the market can cause damage to melanocytes; therefore, a safe and naturally derived skin-whitening agent is needed. Avocado seeds (Persea americana Mill.) are often discarded as waste but possess numerous health benefits. One of the compounds in avocado seeds is catechin, a flavonoid metabolite. This compound has been reported to exhibit antioxidant activity and inhibit tyrosinase to prevent melanin formation, making it suitable for skin-whitening applications. This study aimed to conduct phytochemical screening, assess antioxidant activity using the DPPH method, analyze catechin content using HPLC, and perform cell viability tests using the MTT method from the ethyl acetate extract of avocado seeds. Phytochemical screening has revealed the presence of flavonoids, saponins, and steroids. The DPPH assay yielded an IC50 value of 89.47±0.73 ppm, indicating an intense antioxidant activity, and HPLC detected 0.09% catechin. The MTT test results yielded cell viability percentages ranging from 80-100% at test concentrations of 0.75-25 ppm, with an IC50 value of 61.7 ppm. In conclusion, our results indicate that the ethyl acetate extract from avocado seeds affects cell viability without toxicity, warranting further testing for tyrosinase inhibition in the mouse melanoma B16F10 cell line.
Diversity and Selection of Cotton Germplasm Based on Morpho-Agronomic Character Performance Rahman Side, Taufiq Hidayat; Yamin, Mayasari; Mulyani, Sri; Qadri, Sri Nur; Ayunawati, Luthfi; Aji, Agung Pangestu
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.341-355

Abstract

Cotton is a plant that produces natural fibers of high economic value. Developing a new superior variety of cotton is expected to support fiber demand in Indonesia. The purpose of this study was to determine the diversity of morpho-agronomic characters and obtain secondary characters and cotton accessions that support the development of a new superior cotton variety. The genetic material used was 266 cotton accessions from the ISFCRI germplasm collection. Primary data of the 16 quantitative morphological characters were observed and analyzed using descriptive data, correlation analysis, path analysis, scatterplot, and grouping. The fiber length character has a very low diversity with a value of 0.01, and the plant height character has the highest variance, which is 637.42. The lint percentage with a variance value of 9.37 is influenced by duplicate epistasis genes and controlled by many genes. The fiber's fineness character has a positive and very significant correlation value to the character of the lint percentage. It has fairly high direct and total effect values of 0.156 and 0.134, respectively. Four accession groups can potentially support developing a new superior variety; accession CEA N 268, CEDIX, or L21999-10-71 can be used as the parent of new superior cotton varieties.
Scrutinize the Taxonomical Identity of Green Edible Russula from Sulawesi (Indonesia) Nurhayat, Oktan Dwi; Putra, Ivan Permana; Riffiani, Rini; Taridala, Sitti Aida Adha; Arif, Zulhan
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.436-444

Abstract

Russula Pers. (Basidiomycota) is recognized as one of the most widely distributed macrofungi globally. This genus includes several edible species with distinct color variations, such as R. cyanoxantha. However, in Indonesia, knowledge of this species remains limited as colourful mushrooms are often mistakenly considered poisonous. During a fungus expedition in Southeast Sulawesi, we collected a green edible wild mushroom consumed by locals. Our study aimed to verify the taxonomic classification of our specimens through morphological and molecular analysis. The fresh basidiomata were examined based on their macroscopic and microscopic characteristics. Molecular analysis using ITS 1/2 sequences was conducted to construct a phylogenetic tree. Our findings identified the green mushroom as R. cyanoxantha (Schaeff.) Fr. (Synonym: R. lilacina). Morphologically, our specimens can be distinguished from R. virescens by their smooth cap. They differ from R. aeruginea and R. heterophylla by having flexible, persistent gills. Additionally, our R. cyanoxantha specimen BO24636 can be separated from R. dinghuensis and R. subpallidirosea by their forked gills near the stalk. A BLAST comparison revealed a 96% similarity to R. lilacina from Thailand as the closest match. The phylogenetic tree (Maximum Likelihood) placed our specimens within the R. lilacina group (BS value 99%). Our research contributes to the taxonomic understanding of this edible wild green macrofungi in Indonesia, and future studies should explore its nutritional composition.
Study of Morphology and Growth of Alocasia spp. from Papua, Indonesia Daawia, Daawia; Kartika, Juang Gema; krisantini, krisantini; Rahayu, Megayani Sri; Asih, Ni Putu Sri; Matra, Deden D.; Suhartawan, Bambang
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.367-373

Abstract

Alocasia is a genus of perennial, flowering plants from Araceae, native to tropical and subtropical Asia and eastern Australia. The corms of some Alocasia species are edible, but many Alocasia species are cultivated as ornamentals. Despite the rich biodiversity of Alocasia, there have been limited studies on the morphology and anatomy of these genera. Our study aimed to describe the morphology and growth patterns of two Alocasia species found in Papua, one of the remote regions in Indonesia, A. brancifolia and A. lauterbachiana. The existence of A. lauterbachiana in Papua has never been reported before. The two Alocasia species can be distinguished by their distinct leaf shapes and colors. Alocasia brancifolia has pinnatifid leaves, while A. lauterbachiana has serrated leaves. The leaf of A. brancifolia is thin and predominantly green with faint spots, while the petioles exhibit stripes of brown and green, whereas A. lauterbachiana leaves are thicker and have darker green and reddish color. Due to their attractive leaf shape and colors, both species have the potential to be developed as ornamental potted plants. In terms of growth, A. lauterbachiana exhibited faster growth than A. brancifolia. However, A. brancifolia demonstrated a 50% increase in multiplication over the 16-week study period, while A. lauterbachiana primarily grew taller and larger. This information could prove valuable for future studies to optimize the growth and cultivation techniques of the two Alocasia species for commercial purposes, particularly ornamental foliage plants.
Assessment of Biological Activity, Total Phenolic Content, and Cytotoxicity of Ethyl Acetate Extracts from an Endophytic Fungus, Lasiodiplodia pseudotheobromae IBRL OS-64 Mat Jalil, Mohd Taufiq; Zakaria, Nurul Aili; Yahya, Mohd Fakharul Zaman Raja; Mohamad, Sharifah Aminah Syed; Ibrahim, Darah
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.445-458

Abstract

The biological activities of ethyl acetate extracts of Lasiodiplodia pseudotheobromae IBRL OS-64 were assessed in the present study. Antioxidant activity was measured using the DPPH scavenging assay, antibacterial activity via broth microdilution, and total phenolic content using the Folin-Ciocalteu method. In vivo toxicity was determined using a brine shrimp lethality assay. The fraction extract exhibited the highest antioxidant activity, with an EC50 of 441.6 µg/ml, surpassing the crude extract's. The EEELP showed a relatively low phenolic content of 13.273 µg GAE/mg extract. Regarding antibacterial activity, both crude and fraction extracts demonstrated MIC values ranging from 62.5 to 250 µg/ml against Gram-positive bacteria, while MBC values ranged from 125 to 500 µg/ml for both extracts. The MIC values for crude and fraction extracts against Gram-negative bacteria were 250-500 µg/ml, whilst the MBC values for both crude and fraction extracts were in the range of 500–2,000 µg/ml. The findings also revealed that Gram-positive bacteria were more susceptible to both extracts (crude and fraction) than Gram-negative bacteria. The crude extract exhibited a non-cytotoxic effect with an LC50 value of 2054.88 µg/ml for acute exposure and a low cytotoxic effect with an LC50 value of 199.69 µg/ml for chronic exposure. Meanwhile, the fraction extract demonstrated a non-cytotoxic effect for both acute and chronic with an LC50 value of 5744.14 µg/ml and 1035.94 µg/ml, respectively. Therefore, the present study suggests that the fraction extract could be an effective pharmaceutical agent and safe for drug development due to its low toxicity.
Artificial Neural Networks Model for Photosynthetic Rate Prediction of Leaf Vegetable Crops under Normal and Nutrient-Stressed in Greenhouse Suharto, Yohanes Bayu; Suhardiyanto, Herry; Susila, Anas Dinurroman; Supriyanto
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.300-309

Abstract

Photosynthesis is one of the essential processes in plant physiology that produces glucose and oxygen to support plant growth. Nutrient stress conditions will affect the photosynthetic rate in plants. The model predicting photosynthetic rates based on environmental conditions, nutrients, and plant types will be highly beneficial for farmers in tweaking these variables to maximize plant photosynthesis. This research focused on assessing the impact of nutrient stress on the photosynthetic rate in leaf vegetable crops and aimed to create a model using artificial neural networks (ANN) to predict photosynthetic rates under nutrient-stress conditions. Leaf vegetable crops were cultivated in a greenhouse using the NFT hydroponic system with eight nutrient conditions. This paper introduces an ANN model featuring nine input variables, ten hidden layers, and a single output. This model aims to elucidate the relationship between these inputs and the output parameter. The statistical analysis revealed a notable disparity in the CO2 assimilation rate among leaf vegetable crops subjected to nutrient stress treatment. The constructed ANN model demonstrated strong performance, achieving an R2 value of 0.9416, an RMSE of 1.5898 during training, and an R2 value of 0.9271 with an RMSE of 1.9649 in validation. A combination of statistical analysis and ANN modeling accurately explained the relationship and influence of input parameters, especially nutrient stress conditions, on the photosynthetic rate of leaf vegetable plants cultivated hydroponically in a greenhouse.
Anti-Inflammatory Activity of Phyllanthus niruri L. Leaf Extract: In Vitro Study on RAW 264.7 Macrophage Cells and In Silico Analysis Intan Wiratmini, Ngurah; Pharmawati, Made; Sukmaningsih, Anak Agung Sagung Alit; Joko Raharjo, Sentot; Widowati, Wahyu
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.287-299

Abstract

Phyllanthus niruri L. is a medicinal plant recognized for its wide range of therapeutic benefits, particularly its anti-inflammatory properties. This research focused on assessing the impact of P. niruri leaf extract on pro-inflammatory cytokines in vitro using RAW 264.7 macrophage cells, analyze its phytochemical composition, and validate its potential anti-inflammatory mechanisms through molecular docking studies. The extract was analyzed for its phytochemical composition using LC-MS, revealing the presence of sesquiterpene glycosides, flavonoid glycosides, and tannins among the identified compounds. Cell viability tests showed that the extract was not toxic to the cells at concentrations up to 50 µg/ml. Treatment with the extract significantly reduced the levels of pro-inflammatory cytokines TNF-α and IL-1β in LPS-induced RAW 264.7 cells, with the most effective concentration being 50 µg/ml. Molecular docking studies further supported the anti-inflammatory potential of the extract by demonstrating its ability to inhibit COX-2 and TNF-alpha. These findings suggest that P. niruri leaf extract has promising anti-inflammatory properties and needs further investigation as a potential treatment for inflammatory diseases. The in silico study provided insights into the molecular interactions between the extract's compounds and inflammatory targets, supporting its anti-inflammatory mechanism of action.
Potential Probiotic Yeasts of the Pichia Genus Isolated from ‘Dadih’, a Traditional Fermented Food of West Sumatra, Indonesia Chihombori, Tatenda Calvin; Mustopa, Apon Zaenal; Astuti, Rika Indri; Mutiara, Ilma; Refli, Redoyan; Umami, Rifqiyah Nur; Fatimah; Irawan, Herman; Ekawati, Nurlaili; Trinugroho, Joko P; Akmaliyah, Rizna; Chairunnisa, Sheila; Amani, Febriyanti Nur; Manguntungi, Baso; Hertati, Ai; Mamangkey, Jendri
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.320-340

Abstract

Fermented buffalo milk, known as dadih, serves as a reservoir of potential probiotic yeasts. Over the past two decades, probiotic yeasts have gained increasing attention in both basic and clinical sciences due to their health benefits. This study aimed to isolate and characterize probiotic yeasts from dadih. Yeasts were isolated using yeast Extract, peptone, and dextrose (YPD) medium, and molecularly identified through 18S-rRNA sequencing. Probiotic potential was assessed by evaluating resistance to acidic pH, bile salts, proteolytic, lipolytic, and hemolytic activities. Secondary metabolites produced during fermentation were tested for antimicrobial properties. GBT30 and GBT37 isolates were selected based on their superior performance in probiotic property assays for further analysis. Molecular identification revealed these isolates as Pichia occidentalis (GBT30) and Pichia kudriavzevii (GBT37). Both strains demonstrated in vitro survivability under simulated gastrointestinal conditions and exhibited antimicrobial activity. Whole-genome sequencing of P. kudriavzevii GBT37 identified a genome size of 10,906,850 base pairs, distributed across four chromosomes with a GC content of 38.26%. Notably, secondary metabolite biosynthesis genes were located on contig 7. In addition, 26 probiotic-related genes, including GSY1, HSC82, HSP104, TPS1, ARN1, FLO1, ALA1, SIR2, and others, were identified in P. kudriavzevii GBT37, indicating its potential as a probiotic yeast. The traditional fermentation process of dadih offers probiotic yeasts with promising health benefits, supporting its potential as a functional food.

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