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Indonesian Journal of Applied Research (IJAR)
Published by Universitas Djuanda Bogor
ISSN : -     EISSN : 27226395     DOI : https://doi.org/10.30997/ijar.v1i3
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT
Indonesian Journal of Applied Research (IJAR), e-ISSN 2722-6395 is high quality open access peer reviewed research journal that is published by Universitas Djuanda (UNIDA). IJAR dedicated to publish significant research findings in the field of Applied Sciences, Engineering &Technology. We welcome original empirical research articles with novel perspectives and/or methodological approaches that would appeal to an international readership. We do not accept unsolicited conceptual and theoretical papers. The journal is designed to serve researchers, developers, professionals, graduate students and others interested in state-of-the art research activities in Applied Sciences, which covers four (2) majors areas of research that includes Applied Sciences, Engineering &Technology. The journal was first published in April 2020 and published every April, August and December.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 121 Documents
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In Vitro Study of Piper Crocatum Ruiz & Pav Potency as HMG-CoA Reductase and Malondialdehyde Formation Inhibitor Zaelani, Bella Fatima Dora; Safithri, Mega; Andrianto, Dimas
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.504

Abstract

Coronary heart disease is a serious health problem and it is known to be lethal. The disease can be triggered by the accumulation of cholesterol plaques in the walls of blood vessels This cholesterol formation is assisted by methylglutaril-CoA reductase (HMG-CoA reductase) enzyme. Red betel contains active compounds that have the potential to act as HMG-CoA reductase inhibitor. This study aimed to predict the potential of ethanol extract compounds, water fraction, ethyl acetate fraction, and red betel n-hexane fraction as HMG-CoA reductase inhibitors by in vitro method. The in vitro method which consisted of extraction by maceration, graded fractionation with n-hexane, ethyl acetate, and water, continued by thiobarbituric acid (TBA) method to see the potential antioxidant activity of the sample. The Result showed that the highest inhibition of HMG-CoA reductase is 94% was found in the concentration 200 ppm ethyl acetate fraction. Also antioxidant activity of red betel showed by the inhibition of malondialdehyde formation had the highest activity in the ethyl acetate fraction than other fractions used.
Total Flavonoids, Antioxidant Capacity, and Metabolite Profiling of Red Ginger (Zingiber officinale var. rubrum) Jahira 1 Variety Purwanto, Ukhradiya Magharaniq Safira; Athaya, Ardelia; Septaningsih, Dewi Anggraini; Safithri, Mega
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.512

Abstract

Red ginger (Zingiber officinale var. rubrum) is traditionally recognized for its medicinal properties, largely due to its antioxidant potential attributed to secondary metabolites such as flavonoids. This study aimed to evaluate the total flavonoid content and antioxidant capacity of ethanol and water extracts of red ginger from Sukabumi, specifically the Jahira 1 variety, as well as identify bioactive compounds. Extraction was carried out using the maceration method. The total flavonoid content was determined using the aluminium chloride colorimetric method, while the antioxidant capacity was assessed via the ferric reducing antioxidant power (FRAP) assay. Results showed that the ethanol extract had higher total flavonoid content and antioxidant capacity, with values of 5.99 mg quercetin equivalent (QE)/g and 473.93 µM Trolox equivalent (TE)/g, respectively. In contrast, the water extract exhibited 3.73 mg QE/g and 206.75 µM TE/g. Further LC-MS/MS analysis of the ethanol extract identified 26 compounds, including phenolics and terpenoids, which contribute to the antioxidant capacity of red ginger from Sukabumi.
Isolation and Antagonistic Activity of Actinomycetes Associated with Strawberry Plants (Fragaria x ananassa Duch.) Against Colletotrichum acutatum Simmonds Aji, Oktira Roka; Aisyah, Iis Nisya Nur; Fitria, Karomah Indah
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.519

Abstract

Strawberries (Fragaria x ananassa Duch.) are a valuable horticultural crop in Indonesia, but their productivity is threatened by anthracnose disease caused by Colletotrichum acutatum Simmonds. Traditional control methods often involved synthetic fungicides, which posed risks of pesticide residues and environmental harm. This study investigated Actinomycetes isolated from various strawberry plant organs and their rhizosphere as potential biological control agents. The isolation processes included the collection of Actinomycetes from strawberry plant organs and the rhizosphere, along with the regeneration of C. acutatum. The isolates were identified through observations of their morphological characteristics. Screening of antagonistic activity was performed using dual-culture assays. Over 10 Gram-positive Actinomycetes strains were successfully isolated and characterized. Two isolates exhibited the highest antagonistic activity against C. acutatum, with inhibition percentages of 67.17% and 55.80%, respectively. Data analysis revealed that these isolates demonstrated significant biocontrol potential through mechanisms such as competition and antibiosis. The results suggested that Actinomycetes could serve as effective, eco-friendly alternatives to chemical pesticides in strawberry cultivation. Future research should focus on optimizing these strains for broader agricultural applications.
In Vitro Study of Red Betel Leaves as Lymphocyte Cell Proliferator and Colorectal Cancer Cytotoxic Agent in Widr Cells Pratama, Rahadian; Safithri, Mega; Umar, Mutmainnah Agustiawan; Tarman, Kustiariyah
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.522

Abstract

Colorectal cancer remains a leading cause of mortality worldwide and is closely linked to the immune system, particularly lymphocytes. This study evaluated the ethyl acetate fraction of red betel (Piper crocatum Ruiz & Pav) leaf ethanol extract for its anticancer activity against WiDr colon cancer cells and its effect on lymphocyte cell proliferation. Using MTT assays, the fraction showed significant WiDr cell inhibition (98.18% ± 1.21% at 500 ppm) and enhanced lymphocyte viability (143.3% ± 19.46% at 20 ppm). These results suggest the ethyl acetate fraction serves both as an effective anticancer agent against WiDr cells and as a promoter of lymphocyte proliferation, offering potential for dual therapeutic application.
Antimicrobial Activity of Ethyl Acetate Fraction of Kersen Leaves Against Staphylococcus aureus and Escherichia coli Weni, Mustika; Marfuati, Sri
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.531

Abstract

The underutilized leaves of the kersen plant contain secondary metabolite compounds that have antibacterial potential. The aim of this research was to test the antibacterial activity of the ethyl acetate fraction of kersen leaf against Escherichia coli and Staphylococcus aureus and determine the best concentration. Kersen leaf extraction method using the maceration method using ethanol solvent, fractionation method using ethyl acetate solvent and testing the antibacterial activity of kersen leaf against Staphylococcus aureus and Escherichia coli using the well diffusion method. The normality test used was the Shapiro-Wilk test and used the One-way Anova parametric statistical test. The results of the phytochemical screening of kersen leaf contain compounds phenols, saponins, tannins, alkaloids and triterpenoids. Concentration of 20% of the ethyl acetate fraction of kersen leaf can inhibit Escherichia coli by 8,817 mm in the medium category and Staphylococcus aureus by 7,987 mm in the medium category. The inhibitory power of the ethyl acetate fraction is higher in inhibiting Escherichia coli than Staphylococcus aureus.
Green Synthesis of Silver Nanoparticles Using Extract of Kluwih Leaves (Artocarpus camansi Blanco.) by Microwave-Assisted Irradiation and Their Antibacterial Properties Siregar, Nina Ashariani; Putra, Effendy De Lux; Sinaga, Siti Morin; Satria, Denny; Waruwu, Syukur Berkat
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.540

Abstract

Nanoparticles are particles measuring 1-100 nm that were synthesized using metals, and silver was a commonly used metal because of its potential as an antibacterial agent. Physical and chemical methods were often used in synthesis but are less environmentally friendly. Alternatively, biological processes that utilize phytochemical compounds as reducing agents are preferred. One of the plants rich in phytochemical compounds was kluwih leaves (Artocarpus camansi Blanco). This study aimed to synthesize silver nanoparticles (AgNPs) mediated by kluwih leaves using microwave irradiation. Characterization of nanoparticles was carried out by visual observation and using UV-Vis spectrophotometry. Antibacterial activity was evaluated by determining MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration), followed by examining DNA and protein leakage and biofilm formation using a microdilution method against S. epidermidis and P. aeruginosa. The results showed that AgNPs from kluwih leaves had been successfully formed and could be used as reducing agents in AgNPs biosynthesis. Visual color changed, and analysis of UV-Vis spectrophotometry showed peaks at 441 nm. The results of the antibacterial evaluation showed MIC 1.90 µg/ml (S. epidermidis) and 7.80 µg/ml (P. aeruginosa) also caused cell leakage and reduced biofilm formation significantly at a concentration of 1/2 MIC. These results were promising for further development in future research.
Organic Fertilizer Quality Derived from A Combination of Cow Feces and Empty Fruit Bunche Waste Pancapalaga, Wehandaka; Adiyastiti, Bayu Etty Tri; Rahayu, Imbang Dwi
Indonesian Journal of Applied Research (IJAR) Vol. 5 No. 3 (2024): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v5i3.555

Abstract

To address the accumulation of solid waste from empty oil palm fruit bunches and beef cattle farming waste, handling efforts were undertaken, one of which involved utilizing recycling technology to convert solid waste into high-value organic fertilizer products. This study aimed to assess the quality of organic fertilizer derived from a blend of cow feces and empty fruit bunches (EFB) at various proportions. The materials employed encompassed beef cattle feces and empty fruit bunches (EFB) waste. Employing a completely randomized design (CRD), the study encompassed three treatments: P1 (40% cow feces with 60% EFB), P2 (50% cow feces with 50% EFB), and P3 (60% cow feces with 40% EFB). The quality of the compost measured includes levels of Nitrogen (N), Phosphorus (P2O5), Potassium (K2O), Carbon (C-organic), C/N ratio, and moisture content. Research findings indicated that the quality of organic fertilizer resulting from varied ratios of cow feces and EFB insignificantly influences (p>0.05) Potassium, Phosphorus and C-Organic Conversely, it exhibited a significant impact (p<0.05) on Nitrogen content, moisture content, and the C/N ratio. The average values for organic fertilizer were 0.29% for Potassium, 0.23% for Phosphorus, 31.49% for organic Carbon, 1.80% for Nitrogen, and 42.30% for moisture content. The C/N ratio of the organic fertilizer was determined to be 17.87. In conclusion, organic fertilizer derived from a combination of EFB and cow feces complies with the Indonesian National Standard (SNI 19-7030-2004). The optimal ratio for utilizing EFB and cow feces in organic fertilizer production was determined to be 1:1.
Optimization of Agro-Industrial By-Product-Based Feed as a Substitute for Commercial Feed in Starter Pigs Pongtuluran, Ardi Matutu; Wiryawan, I Komang G; Salundik; Negara, Windu
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 1 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i1.566

Abstract

The high feed price, mainly commercial feed, made it unaffordable for smallholder-scale pig farmers in Toraja. As a result, rations were given to pigs without considering nutritional content. This study aimed to substitute commercial feed with agro-industrial by-product-based feed in starter pigs. A total of 20 local breeding pigs, aged 13 weeks, were tested using a randomized group design consisting of 4 treatments with 5 replicates. The treatments were P0 = 100% commercial feed as control; P1 =substitution of 50% with agro-industrial by-product feed; P2 = substitution of 100% agro-industrial by-product feed; P3 = P2 + phytase and mannanase enzymes. ANOVA and Duncan test were performed for analysis the data. The results showed that substituting commercial feed with agro-industrial by-products feed had no negative impact on dry matter (DM), organic matter (OM), and crude protein (CP) intake. However, substituting commercial feed with agro-industry feed significantly decreased the digestibility of feed nutrients. The lowest digestibility values were obtained from the 100% agro-industrial by-product-based feeds at 77.81%, 80.17%, and 38.15% for DM, OM, and neutral detergent fiber (NDF), respectively. Interestingly, adding the enzyme mannanase and phytase (P3) significantly increased the digestibility values of DM, OM, and NDF to 82.79%, 85.14%, and 53.67%. This study also showed no effect of commercial feed substitution up to 50% on the performance of pigs in the starter phase. In addition, this practice can also increase farmers' income by up to 15%.
The Nursing and Respiratory Behaviour of The Bottlenose Dolphin Calf (Tursiops aduncus) in Captive Breeding Setiadi, Dedi Rahmat; Hastuti, Yohana Tri; Amani, Aqila Zata; Laksono, Mohamad Nanang Tejo; Manansang, Jansen; Mulia, Bongot Huaso; Manansang, Willem; Arifiantini, Raden Iis
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 1 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i1.585

Abstract

Bottlenose dolphins were often found in zoos and aquariums. Dolphin calves have a higher mortality rate in the first month after birth as they are unable to eat and breathe properly. The inability to thrive can affect growth until adulthood. This research was being conducted to better understand the nursing and breathing patterns of a bottlenose dolphin calf. Data were collected by observing the behaviour of a post-partum dolphin calf for 24 hours over 3 months (90 days). The behaviours observed were the calf's nursing (frequency, daily duration, and duration of suckling) and breathing (frequency). The results showed a decrease in the frequency of suckling and an increase in the duration of suckling, which indicated that the calf was becoming more successful at suckling. A constant decrease in respiratory frequency indicated a healthy growing calf due to an increase in lung capacity. These results indicated that the feeding and respiratory behaviour patterns of normally developing bottlenose dolphin calves showed a decrease in feeding frequency and an increase in feeding duration, as well as a decrease in respiratory frequency per week. Further studies on the feeding and respiratory behaviour of bottlenose dolphin calves.
The Cells Growth of Saccharomyces cerevisiae in Substrates of Lignocellulosic Hydrolysate-Added Hydrogen Peroxide Using Turbidimetric Method Syauqi, Ahmad; Saada, Iqlimatus; Trisna, Adira Tatus; Rodiyanto
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 1 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i1.598

Abstract

Ethanol was produced reliably using the S cerevisiae yeast for biorefinery as a new renewable energy source (RES). The abundance of lignocellulosic waste (β-glucan) requires its utilization and can be directed to bioethanol-RES production. We focus on hydrolysed polysaccharide fibre from biomass waste that was pretreated with hydrogen peroxide and hydrolisate used for cell growth in the early stages of fermentation activity. The research aims first to compare the initial stages of growth of the Saccharomyces cervisiae cell population in media from the Gracilaria sp macroalgae waste. Second, the consistency of the S cerevisae growth method, namely turbidimetry and cell mass in the early stages of fermentation activity. The research used an experimental method with independent variables as control treatment factor: corn polysaccharide extract (α-glucan 18), lignocellulosic hydrolysate-added peroxide 0.1%, and sugar cane disaccharide granules, seven repetitions for treatment respectively. The control variable was temperature and agitation. Growth parameters used turbidity-cell number and the cell mass tradition for sugar cane disaccharide granules. The determinant of cell number growth according to time is 74.44%; it was estimated that the influence of corn polysaccharides. Lignocellulosic hydrolysate substrate provides growth with a positive slope with a determinant of 39.9%, and both regression slopes were not significantly different and were exponential, while the use of sugar cane disaccharides had a negative slope. The lignocellulosic hydrolysate had a positive potential medium for the growth of Saccharomyces cerevisiae in the early stages of ethanol fermentation.

Page 10 of 13 | Total Record : 121

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