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INDONESIA
Bioscientist : Jurnal Ilmiah Biologi
ISSN : 23385006     EISSN : 26544571     DOI : https://doi.org/10.33394
Bioscientist : Jurnal Ilmiah Biologi (BJIB), receiving and publishing article in the form of research (scientific article) in the field of biology education and utilization of biological research in learning. Moreover, this journal bridges the gap between research and practice, providing information, ideas and opinion, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. Special emphasize are as follow: 1. Research on Learning Biology (Biology Learning Materials at All Education Levels). 2. Pure Research of Biology Developed or Studied to the Sources, Materials, or Instructional Media Biology (Biology Learning Materials at All Education Levels and Application in Society). 3. Curriculum of Biology Education at all Education Levels. 4. Class Action Research (CAR) and Lesson Study in Biology. 5. Other Qualitative Research of Biology Education. 6. The School Management and Biology/Science Laboratory Management. 7. Biology Learning Evaluation. 8. Teacher Professional Issues/Trends in Biology Education. 9. Another Study for the Scope of Biology Education. 10. Research on Environmental Education.
Articles 1,428 Documents
Produksi Kotoran Cacing Tanah dan Hubungannya dengan Kelimpahan Cacing Tanah pada Ekosistem Alami dan Ekosistem Buatan Regina Jody; Darmi Darmi; Rizwar Rizwar
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11550

Abstract

The presence of earthworms in an ecosystem can improve soil quality through physical, chemical and biological properties. The activity of earthworms in producing feces also has an important role in increasing soil fertility. This research aims to analyze the production of earthworm feces and its relationship with the abundance of earthworms in natural and artificial ecosystems. Determining location points uses the Cluster Sampling method, namely natural and artificial ecosystems. Sampling of earthworms and their feces used the line transect method with a length of 100 meters and plots were created along the transect lines to observe the population and production of earthworm feces with 20 replicate plots at each location. Data analysis includes average feces weight, number of earthworm feces, and correlation analysis. Morphological data on earthworm droppings were analyzed descriptively. The results of this research show that in natural ecosystems there are 2 forms of earthworm feces, namely granular and globular types, whereas in artificial ecosystems there is only 1 form of earthworm feces, namely globular. Earthworm feces production is higher in natural ecosystems than in artificial ecosystems. The relationship between dung production (number of deposits and weight of dung deposits) of earthworms with the number and biomass of earthworms shows a significant relationship with a moderate correlation category (<1), while the relationship between earthworm density and worm biomass correlates significantly with a strong correlation category (1).
Penetapan Kadar Kuersetin pada Obat Herbal yang Mengandung Ekstrak Tempuyung (Sonchus arvensis) Menggunakan KLT Densitometri Asih Imulda Hari Purwani; Krisna Kharisma Pertiwi; Dwi Wahyuni; Rachma Nurhayati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.11260

Abstract

Quercetin are the main marker compounds in extracts of Sonchus arvensis which show nephrolithiasis activity. For quality control of herbal medicines, Densitometric Thin Layer Chromatography (TLC) method has been developed in this study using quercetin as phytochemical markers. Objective: Determination for quercetin in herbal medicines. Determination of quercetin by determining the composition of the mobile phase, the wavelength of the analysis, the saturation time, the elution distance and the volume of the spot. The parameters observed in the optimization method and stability test are area, retardation factor (Rf), peak shape and resolution (Rs). The composition of selected mobile phase which results good separation and symmetrical peak shape is chloroform: methanol: dichloromethane: acetonitrile: formic acid (6: 2: 2: 0.05: 0.05 v/v/v/v/v) with a wavelength of 335 nm, time of saturation is 30 minutes, elution distance is 8 cm, volume of spots is 10 µL and Rf 0.48. In this study, it was shown that there were no factors that had a significant effect so that the method could be applied for the determination of quercetin levels in herbal medicines.The research data showed that the range of levels on quercetin 3.26%.
Kadar Silika (SiO2) terhadap Kelimpahan Diatom Bentik di Sungai Jamblang Kabupaten Cirebon Maharani Zahiratun Haque; Norma Afiati; Oktavianto Eko Jati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9192

Abstract

Jamblang River is a concern because it has a very turbid water color due to natural stone mining activities processed by the natural stone industry. One of the natural stones produced at the location of the Jamblang river is andesite rock which has a constituent component is silica. Silica is the second most abundant element in the Earth's crust after oxygen. Silica usually comes from minerals and rocks that dissolve in water. In fresh water, quartz silica is less soluble than amorphous silica. Enrichment of nutrients with silica favors the growth of diatoms, since diatoms need silica for cell wall biogenesis. The amount of silica present in water is not immediately absorbed by diatoms. The sampling method used is the purposive sampling method. The sampling locations set are 4 locations. The most interesting location is location 2 because there are 38 natural stone industries scattered around the location. There is also a simple linear regression statistical test on SPSS which shows the influence of silica levels on the abundance of benthic diatoms in the Jamblang river. The value of Sig. 0.000 shows that there is an influence of silica on plankton abundance in water because the value is < 0.05. The lowest silica content obtained at location 2 is also supported by the least abundance of diatoms found. The low abundance of diatoms is thought to be caused by high TSS numbers, low observed brightness, and low DO numbers.
Evaluasi Kandungan Fitokimia, Kapasitas Antioksidan, dan Toksisitas Ekstrak Daun Stevia rebaudiana Frankson, Desvin; Hendrawan, Siufui; Ferdinal, Frans
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12151

Abstract

Oxidative stress leads to numerous clinical problems, such as autoimmune, vascular and respiratory impairment, as well as many other degenerative diseases. While daily exposure to UV light, air pollution, and smoke largely contribute to oxidative stress, endogenous antioxidants mostly fail to neutralize these high amounts of free radicals. Stevia rebaudiana or “candyleaf” is largely found in Indonesia, known for its application as a natural sweetener, owing to its content of stevioside compound. This study is experimental research which aims to assess the potential of stevia extract as an antioxidant supplement, which has not been widely applied. In vitro study was performed by extracting stevia leaves in methanol through simple maceration and percolation methods. Subsequently, the stevia extract was subjected to biochemical analysis, i.e. phytochemical screening, total phenolic content assay, antioxidant capacity assay, and toxicity assay. Herein, we found that stevia extract contains flavonoid, saponin, tannin, terpenoid, and phenolic compounds, with total phenolic content of 86.30±1.14 mg GAE/g DW. Moreover, antioxidant capacity assay through 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method showed that IC50 of stevia extract is 20.13 µg/mL, slightly higher than Trolox (vitamin E analog) with IC50 of 16.50 µg/mL, which indicated that stevia extract is a very strong antioxidant. As for toxicity bioassay, Brine Shrimp Lethality Test indicated that stevia extract is non-toxic with LC50 value of 3388.44 µg/mL.
Teknik RAPD (Random Applied Polymorphic DNA) untuk Analisis Keanekaragaman Genetik pada Tanaman Ciplukan (Physalis angulata L.) di Kabupaten Labuhan Batu Utara Yusvita, Diana; Idami, Zahratul
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12889

Abstract

The ciplukan plant (Physalisangulata L.) can be used as traditional medicine, but so far people only know that this plant can only be used for the fruit. This research aims to determine the results of RAPD primary analysis which can produce polymorphic bands in Ciplukan Plants (Physalisangulata L.) in North Labuhan Batu Regency using different primers. This research is a quantitative research. Sampling was carried out in Bondar Village and Simpang Tiga Village, Kualuh Leidong District, North Labuhan Batu Regency. Then continued with molecular analysis at the Genetics Laboratory, Faculty of Science and Technology, North Sumatra State Islamic University, Medan. The results of RAPD (Random Applied Polymorphism DNA) analysis of ciplukan plants in North Labuhan Batu district using six different primers, namely the length of the nucleotides in the amplified DNA fragments, are shown in primers OPA-2 & OPA-3 are 600bp-3000bp, while OPA-5, OPA-7, OPD -11, & OPD-13 are 500bp-3000bp with different nucleotide sequences. The differences in the length and number of nucleotide sequences in ciplukan plants are caused by the plants used coming from different locations and several factors such as the environment, population size, natural conditions, reproductive methods and natural selection.
Isolation and Identification of Bacteria Producing Antibiotic Compounds from The Rhizosphere of Avicennia Marina Against Pathogenic Bacteria in The Mangrove Ecosystem Area of West Kalimantan Khairillah, Yuyun Nisaul; Alang, Hasria; Haryanto, Haryanto; Fitriagustiani, Fitriagustiani; Erwhani, Indri; Purnamawati, Ditha Astuti; Rahmawati, Annisa; Surtikanti, Surtikanti; Triwahyuni, Adelia
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12527

Abstract

Antibiotic resistance is a global problem in the health sector. The level of antibiotic resistance that occurs can be caused by several influencing factors, including 6 isolates of rhizosphere bacteria that were successfully isolated from the rhizosphere of the Avicenia marina plant. The purpose of this study was to isolate and determine the best minimum inhibitory concentration of rhizosphere bacteria of the A. marina plant and its effectiveness in inhibiting pathogenic bacteria S. aureus. This study is a quantitative research method using the isolation and characterization of rhizosphere bacteria, antagonistic test of rhizosphere bacteria and test of the results of the extraction of filtrate of rhizosphere bacteria culture of A. marina plants. The results of this study were (1) five of the isolates of bacteria that were successfully isolated were included in the Bacilus type and 1 isolate of bacteria included in the Cocobasil type; (2) there were 3 of the 6 isolates that could inhibit the growth of pathogenic bacteria S. aureus with an inhibition range of 10.04-4.42 mm; (3) Rhizosphere bacterial isolate (TN 1) is an isolate with the best inhibition diameter and is categorized as strong, namely 10.04 mm; (4) ethyl acetate extract of Rhizosphere bacterial isolate culture filtrate (TN 1), has a minimum inhibitory concentration of 0.50% against pathogenic bacteria S.aureus with an inhibition diameter of 7.18 mm which is better when compared to the positive control using chloramphenicol.
Analisis Beban Kerja dan Stres Kerja pada Perawat Muhajirin, Fahmi Abu Rizal; Sulaiman, Lalu; Setiwan, Sabar
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12970

Abstract

Perawat merupakan salah satu profesi di bidang kesehatan yang memiliki tanggung jawab besar dalam memberikan perawatan kepada pasien. Di Puskesmas, perawat seringkali menghadapi beban kerja yang tinggi akibat jumlah kunjungan pasien yang banyak serta tugas administratif tambahan. Beban kerja yang tinggi ini dapat mempengaruhi kesejahteraan psikologis perawat, menyebabkan stres kerja yang berpotensi menurunkan kualitas pelayanan kesehatan.  Penelitian ini bertujuan untuk menganalisis hubungan antara beban kerja dan stres kerja pada perawat di Puskesmas Tanjung, Kabupaten Lombok Utara. Studi ini merupakan penelitian kuantitatif dengan pendekatan survei. Sampel dalam penelitian ini sebanyak 41 perawat yang ditetapakan menggunakan teknik purposive sampling. Instrumen penelitian terdiri dari kuesioner beban kerja dan kuesioner stres kerja yang menggunakan skala Likert. Analisis data penelitian menggunakan uji kolerasi Pearson. Hasil analisis menunjukkan bahwa terdapat hubungan positif dan signifikan antara beban kerja dan stres kerja, dengan nilai koefisien korelasi (r) sebesar 0.65 dan p-value sebesar 0.000. Hal ini mengindikasikan bahwa peningkatan beban kerja berkorelasi dengan peningkatan tingkat stres yang dialami oleh perawat.
Potensi Bakteri Endofit dari Tanaman Jahe Sebagai Agens Pengandali Hayati Kristianingrum, Sabrina Ayu; Setiawan, Andree Wijaya; Jayanti, Ruth Meike
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.12263

Abstract

The use of synthetic pesticides is not effective in reducing the prevalence of disease because it produces residues and does not support biodiversity. This is an unwise practice and can cause problems in the future.  The use of endophytic microorganisms is widely used to control plant diseases and pests, because it is known to have various pathogen inhibition mechanisms. Biological agents that are used to inhibit plant pathogens and have been widely developed, one of which is endophytic bacteria. The purpose of the research was to evaluate the potential of endophytic bacterial isolate from ginger plants as biological control agents. The type of research used is descriptive and exploratory. Sampling of healthy ginger plants was carried out at 5 points in two sub-districts, namely Getasan and Sumowono, Semarang Regency. Isolation is carried out by taking healthy ginger plants from the roots, rhizomes, fronds, and leaves. Characterization and selection of isolates were carried out by testing their phosphate dissolving ability, nitrogen anchoring, amylase and protease activities, and antagonist tests with Fusarium oxysporum in vitro. The results of this study are that (1) 20 pure isolates were successfully isolated consisting of 2 isolates derived from leaves, 4 isolates from fronds, 8 isolates from roots, and 6 isolates from rhizomes; (2) D91 isolate has the potential as a biological agent with an antagonist mechanism of 86.91% supported by protease enzyme activity, able to dissolve phosphate and anchor nitrogen.
Lama Fermentasi dan Konsentrasi Bunga Chamomile (Matricarica recutita) Terhadap Mutu Fisikokimia dan Organoleptik Teh Kombucha Fahilah, Khoiriyah; Hartati, Fadjar Kurnia; Yuniati, Yuyun
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.14182

Abstract

Kombucha is a health drink that has good benefits for the body, contains polyphenols and is antioxidant so it is said to be a functional drink. Chamomile positively contains flavonoid compounds, polyphenols, and tannins as antioxidants to ward off free radicals. The purpose of this study was to determine the effect of fermentation duration and concentration of chamomile flowers (Matricaria recutita) to produce kombucha drink with the best physicochemical and organoleptic quality. This study used a one-factorial Completely Randomized Design consisting of four treatments of a combination of fermentation duration (5 days and 7 days) and chamomile concentration (0.5% and 1%), each treatment was carried out three times. Parameters analyzed included color (L*a*b*), pH, antioxidant activity and organoleptic (taste, color, and aroma). The results showed that the length of fermentation and chamomile concentration had a significant effect on color (L*a*b*), pH, antioxidant activity and organoleptic parameters (taste and color). On organoleptic parameters (aroma) did not have a significant effect. The best treatment with antioxidant activity value of 67.09% in treatment F2C2 (7 days fermentation time and 1% chamomile concentration). The highest organoleptic quality was in the F1C1 treatment (5 days fermentation and 0,5% chamomile concentration).
Macrozoobenthos Diversity in The Fisheries Mangrove Ecotourism Area in Pulau Banyak Village Langkat District Manullang, Helentina Mariance; Lubis, Zulkarnain; Sabrina, Raja
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.13660

Abstract

Mangroves are important ecosystems located in tidal areas and become habitats for various types of biota, including macrozobenthos. Macrozobenthos is a group of animals that inhabit the bottom waters of mangrove ecosystems. This study aims to analyze the diversity of macrozobenthos species in the mangrove ecotourism area of Pulau Banyak village, Langkat Regency, North Sumatra. This research was conducted from October 2022 to October 2023 in the fisheries-based mangrove ecotourism area in Pulau Bayak village, Langkat Regency. The research location was divided into three research stations. Macrozobentos observations were made once a month. Sample collection is done by collecting directly by hand, the samples collected are on the sample map (plot) measuring 1 x 1 meter making as many as 10 pieces for every 100 m2. Data were analyzed using the Paleontological Statistic (PAST) Version 4.0 tool. Parameters studied include diversity index, evenness, species richness, and dominance. Based on the results of the analysis, it is known that the diversity index is in the medium category with a value of 2.4-2.5, while the evenness index is in the high category with a value of 0.91 - 0.92, then the species richness index is in a low category with a value of 2.20 - 2.22 and the dominance index shows that there are no certain species that dominate this ecosystem with an index value close to 0. Thus, it can be concluded that this fisheries-based mangrove ecotourism area is in good condition and can support the lives of organisations associated with mangrove ecosystems.

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