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Sri Agus Sudjarwo
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INDONESIA
journal of Basic Medical Veterinary
Published by Universitas Airlangga
ISSN : 23026820     EISSN : 27749959     DOI : https://dx.doi.org/10.20473
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, Pathology, Microbiology, Pharmacology and Medical Education.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 114 Documents
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Most Probable Number of Coliform on Beef from Krian Abattoir, Sidoarjo Pratiwi, Sambang Dewi; Harijani, Nenny; Sarudji, Suryanie; Budiarto, Budiarto; Estoepangestie, Soelih
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36476

Abstract

The problem on food health is the high amount of bacterial contamination of food served by food industries. The quality of beef can be affected by the level of bacterial contamination in meat. Coliform is a group of bacteria used as a sanitary hygiene indicator. The purpose of this study is to measure the value of MPN Coliform in beef at Krian's abattoir of district Sidoarjo. Thirty samples were taken from the abattoir of Krian, Sidoarjo. Most Probable Number was used to determine the level of Coliform's contaminate beef. The results showed that all beef samples at abattoir were contaminated with Coliform. The average contaminant levels was 706,76 MPN/g. Twenty six samples exceed the maximum limit of Coliform bacteria contamination in accordance with Indonesian National Standard (SNI). The highest Coliform MPN value is ≥ 1600 MPN / g, whereas the lowest MPN Coliform value is 8 MPN/g.
The Effect of Mimosa Pudica Root Extract on Cerebrum Histopathological of Rattus Norvegicus Induced with Naja Sputatrix Venom Permatasari, Roselia Yuliani; Hestianah, Eka Pramyrtha; Legowo, Djoko; Rachmawati, Kadek; Arifin, Zainal
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36485

Abstract

The aim of this study was to know the effect of Mimosa pudica root extract on histopathological appearance of Rattus norvegicus brain induced by Naja sputatrix venom. Thirty rats were divided into 5 groups. There were 2 control groups and 3 treatment groups, which was given 250, 500, and 1000 mg/kg BW of Mimosa pudica root extract orally. The first 7 days each group was adapted to the environment. On the 8th day, the treatment was started by injecting Naja sputatrix LD50 (0,13 L/gram BW) IM in gluteus muscle, continued with giving Mimosa pudica root extract orally for the treatment groups 5 minutes after venom injection. 6 hours after the last treatment, rats were killed by cervical dislocation, injected with formalin 10% in the heart, then necropsied. Histopathological evaluation was done to score brain damage based on meningitis, perivascular cuffing, and necrotic cells using HE stain with 1000x magnification. The result showed 1000 mg/kg BW dosage of Mimosa pudica root extract can reduce brain damage based on meningitis, perivascular cuffing, and necrotic cells in Rat (Rattus norvegicus) caused by Naja sputatrix venom and gave significant difference (p < 0.05) among the treatment groups.
The Efficacy of Putrimalu Root Extract (Mimosa pudica) Against Mortality and Histopathological of Rat Muscles Injected Naja sputatrix Venom Rendragraha, Briantono Willy; Legowo, Djoko; Kuncorojakti, Suryo; Chusniati, Sri; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.36486

Abstract

The purpose of this experiment was to know the effect of Mimosa pudica root extract (normal water extraction) on mortality and muscle histopatological in experimental animal (Rattus norvegicus) which injected with LD50 of Naja sputatrix venom. Fresh Mimosa pudica root dried without sunshine and ground into coarse powder with steamroller (mash 3mm). Plant extract were prepared by stirring 4 g of the powder into 200 ml of water for 3 hours at room temperature and filtered with muslin cloth before freezdried. 30 rat divided into five groups, P0(C+), P1(C-) P2, P3, and P4. P0 (positive control group) injected by saline intra muscular and given aquadest peroral after five minutes, P1 (negative control) injected with Naja sputatrix venom and given aquadest peroral after five minutes. P2, P3, and P4 injected by Naja sputatrix venom and given Mimosa pudica extract with 250mg/KgBW, 500mg/KgBW, and 1000mg/KgBW doses. All of the experimental animal observed about six hours and than euthanized, musculus gluteus maximus (location of injection) was taken for histopatological examination. In this experiment observed that the normal water extraction of mimosa pudica can't reduce mortality of experimental animals but it can decrease muscle damage in 1000mg/KgBW dose.
Epidemiological Study of Suspected Occurrence of Foot and Mouth Disease in Lamongan Regency Sudarsono, Rahendra Prasetya Eko
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.37197

Abstract

Lamongan is the epicenter of aphtae epizootica disease. Symptoms of the disease appeared from the newly purchased cattle in Balungpanggang District, Gresik Regency. Symptoms that appear hypersalivation, decreased appetite, panting, slightly feverish body temperature. This review was conducted by collecting primary data through field observations and supported by references from the Google Scholar database with the keywords foot and mouth diseases, aphtae epizootica and infectious diseases. The pattern of distribution is mainly cattle traffic, weak biosecurity in cattle pens and livestock transportation means for sending cattle from or to the animal market. Based on a descriptive analysis of the results of surveillance, investigation and sample testing indicating an outbreak of an infectious disease suspected to be FMD in Lamongan Regency according to the definition of an outbreak according to Law No. 18 of 2009 concerning Livestock and Animal Health and Government Regulation No. 47 of 2014 concerning Disease Control and Prevention. Animal. The direct impact in the form of economic losses due to illness and death if the outbreak is not controlled is estimated to be quite significant.
Safety Evaluation of Lawsonia inermis on Physiological, Andrological and Haematological Parameters of Male Wistar Rats Aremu, Abdulfatai; Oridupa, Olayinka Ayotunde; Akorede, Ganiu Jimoh; Olatumji, Aishat Omobolanle; Basiru, Afisu; Ahmed, Akeem Olayiwola; Raufu, Ibrahim Adisa
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i2.32483

Abstract

The leaves of Lawsonia inermis Linn are used in the treatment of many diseases such as diabetes, poliomyelitis, measles and gynecological disorders such as menorrhagia, vaginal discharge and leucorrhoea. This study was designed to investigate the safety evaluation of Lawsonia inermis Linn leaves (acutely and sub-chronically) on physiological, biochemical and histopathological changes seen in Wistar rat. Acutely, female rats were divided into four groups (n=3) and treated as thus A (untreated control); B (1000 mg/kg); C (2000 mg/kg) and D (5000 mg/kg). Sub-chronically, 25 male Wistar rats were grouped into five (n=5). Groups: A (control), B (100 mg/kg); C (200 mg/kg); D (400 mg/kg) and E (800 mg/kg). Lawsonia inermis Linn leaves have a wide safety margin (>5000mg/kg) and no mortality or visible toxic reaction was observed in acute phase. Lawsonia inermis extract did not inhibit physiological weight gain, except the highest dose that caused some weight loss. Haematological result showed that PVC, RBC, haemoglobin and platelets had no significant (P>0.05) effect unlike white blood cell and differentials (neutrophils, lymphocytes and monocytes) which decrease significantly (P <0.05) across all the treated groups compared to untreated control. Serum chemistry showed a significant (P <0.05) decrease AST. ALT, ALP, creatinine, urea, Total protein and Total bilirubin had no significant (P <0.05) effects. Serum electrolytes; calcium ion, potassium ion, sodium ion and chloride ion had no significant (P <0.05) changes. Lawsonia inermis is safe at acute administered dosages while nephrotoxicity and spermiotoxicity may occur following subchronic administration.
Histopathological Overview of Kidney Given Extract of Thistle Leaf (Calotropis gigantea) and Aspirin® Inducted to White Rats (Rattus norvegicus) Joharry Leto Bele, Alexander
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i2.39703

Abstract

This research aimed to determine the overview of gastric histopathology that contained necrosis, degeneration and inflamed-cell infiltration in gastric muccose that inducted by aspirin and widuri leaves (Calotropis gigantean) ekstract in white rat (Rattus norvegicus). The experimental animals that used in this research were 25 white rats that selected by simple random sampling technic. The design that used in this research was complete random design that devided into 5 groups of treatment and 5 repetition in each groups. The treatment group were P0 = negative control group, P1 = positive control group that gave aspirin 500 mg/kg body weight, P2 = was the group that given aspirin 500 mg/kg body weight and widuri leaves extract 50 mg/kg body weight, P3 = was the group that given aspirin 500 mg/kg body weight and widuri leaves extract 100 mg/kg body weight, P4 = was the group that given aspirin 500 mg/kg body weight and widuri leaves extract 150 mg/kg body weight. Data that collected analyzed by Kruskal-Wallis statistic test and Mann- Whitney Test. Based on the by Kruskal-Wallis Test and Mann-Whitney Test, p (Asymp.sig) < 0.05. That means there was the real different between groups of treatments. The conclusion of this research was the dosis 100 mg/kg body weight dan 150 mg/kg body weight dose of widuri leaves extract could decrease the gastric necrosis, degeneration and inflammation.
Histopathological Overview of Gastric Inducted by Aspirin® and Given Extract of Thistle Leaf (Calotropis gigantea) on Albino Rats Riong, Kornelia Karolina
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i2.39770

Abstract

This research aimed to determine the overview of gastric histopathology that contained necrosis, degeneration and inflamed-cell infiltration in gastric muccose that inducted by aspirin® and widuri leaves (Calotropis gigantean) ekstract in rat. The experimental animals that used in this research were 25 rats that selected by simple random sampling technic. The design that used in this research was complete random design that devided into 5 groups of treatment and 5 repetition in each groups. The treatment group were P0 = negative control group, P1 = positive control group that gave aspirin® 500 mg/kg body weight, P2 = was the group that given aspirin® 500 mg/kg body weight and widuri leaves extract 50 mg/kg body weight, P3 = was the group that given aspirin® 500 mg/kg body weight and widuri leaves extract 100 mg/kg body weight, P4 = was the group that given aspirin® 500 mg/kg body weight and widuri leaves extract 150 mg/kg body weight. Data that collected analyzed by Kruskal-Wallis statistic test and continued by Mann-Whitney Test. Based on the result of Kruskal-Wallis and Mann-Whitney Test, (p<0.05). That means there was the real different between groups of treatments. The conclusion of this research was the dosis 100 mg/kg body weight dan 150 mg/kg body weight dose of widuri leaves extract could decrease the gastric necrosis, degeneration and inflammation.
Effect of Plectranthus scutellarioides (L.) Leaf Extract as Natural Antibacterial Against Staphylococcus aureus and Escherichia coli Isolated From Dairy Cattle with Subclinical Mastitis Shafina Hanum, Farah; Witaningrum, Adiana Mutamsari; Puspitasari, Yulianna
Journal of Basic Medical Veterinary Vol. 11 No. 2 (2022): Journal of Basic Medical Veterinary, December 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i2.39799

Abstract

Mastitis is one of the causes of low milk production in dairy cows, and can be caused by bacteria such as Escherichia coli and Staphylococcus aureus. Plectranthus scutellarioides (L.) leaf has antibacterial compounds such as flavonoids, eugenol, polyphenols, steroids, tannins, and so on. The type of this research is experimental laboratory research that aims to test the antibacterial activity of the P. scutellarioides (L.) leaf extract with ethanol pro analysis as the solvent against E. coli and S. aureus bacteria isolated directly from the sample of dairy cow's milk which CMT score is (+) 3, by measuring its inhibition ability indicated by the presence of clear zone using the agar-well diffusion method. The extract concentrations tested are 5%, 10%, 20% and 40%. The result of the antibacterial test of P. scutellarioides (L.) leaf extract with ethanol p.a. as the solvent showed antibacterial activity against E. coli and S. aureus bacteria isolated directly from cow's milk samples with subclinical mastitis at certain concentrations.
Screening of Blood Glucose Concentration in Domestic Cat (Felis Catus) Based on Body Condition Score, Breed, and Sex Using Portable Blood Glucose Meter Billa, Lutfiah Annisa; Triakoso, Nusdianto; Rachmawati, Kadek; Yuniarti, Wiwik Misaco; Aksono, Eduardus Bimo; Yudaniayanti, Ira Sari
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42246

Abstract

Type 2 diabetes mellitus (T2DM) is a common endocrine disease in domestic cats. Breed, sex, being overweight to an extent of obesity are predisposing factors for developing T2DM. One of the most common laboratory analyses conducted to confirm this disease is blood glucose concentrations. The aim of this research is to determine blood glucose concentration in domestic cats based on BCS, breed, and sex using a portable blood glucose meter. Research samples were obtained by taking blood samples from the marginal ear vein of 131 domestic cats that matched the sample's criteria, took at 2-hours post prandial, and drip onto the Sinocare strip. The results showed that the average blood glucose concentration was in the normal range, with a total of ideal (BCS ≥3/5) domestic cats (n=43) was 75.74 mg/dL, overweight (BCS ≥4/5) domestic cats (n=44) was 78.23 mg/dL, obese (BCS ≥5/5) domestic cats (n=44) was 89.66 mg/dL, male (n=51) was 80.57 mg/dL, female (n=80) was 81.69 mg/dL, Mixed Breed (n= 119) was 81.03 mg/dL, Angora (n=5) was 84.20 mg/dL, Persian (n=6) was 81.50 mg/dL and Ragdoll (n=1) was 92 mg/dL. Pearson correlation showed BCS (r=0.403; p= 0.000) had a moderate, positive, and significant correlation with blood glucose concentration in domestic cats. Meanwhile, the results of Kendall's Tau correlation showed that breed (p=0.740) and sex (p=0.555) had insignificant correlation with blood glucose concentration in domestic cats.
The Effectiveness of Ethanol Extract of Ketapang Leaves (Terminalia catappa L.) as a Larvicidal Against Aedes aegypti Mosquito Larvae Dwi Ananda, Novita; Rachmawati, Kadek; Lastuti, Nunuk Dyah Retno; Suwanti, Lucia Tri; Hidajanti, Nove; Meles, Dewa Ketut
Journal of Basic Medical Veterinary Vol. 12 No. 1 (2023): Journal of Basic Medical Veterinary, June 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i1.42248

Abstract

This study aimed to determine the effectiveness of the ethanol extract of ketapang leaves (Terminalia catappa) on mortality and histopathology of Ae. aegypti instar III as an effort to control Ae. aegypti as a disease vector without causing resistance to the larvae. The extraction process uses the maceration method. This type of research is a laboratory experiment with research subjects Ae. aegypti instar III of 700 individuals, which were divided into 7 treatment groups (1 negative control group using distilled water and 1% DMSO, 1 positive control group using 1000 ppm abate, ethanol extract of ketapang leaves with concentrations of 2000 ppm, 2500 ppm, 3500 ppm, 5000 ppm, 7000 ppm) and 4 repetitions. The results of this study indicate that the negative control (K-) mortality rate is 0% and the positive control (K+) mortality percentage is 100%. The results of the ethanol extract of ketapang leaves (Terminalia catappa) on the mortality of Ae. aegypti instar III in treatment one (P1) the percentage of mortality was 17%, treatment two (P2) the percentage of mortality was 21%, treatment three (P3) the percentage of mortality was 23%, treatment four (P4) the percentage of mortality was 27%, and treatment five (P5) the percentage of mortality is 42%. Histopathological observations on larvae that died after being exposed to ethanol extract of ketapang leaves (Terminalia catappa) for 24 hours damaged the basement membrane, peritropic membrane and epithelial cells. It can be concluded that the ethanol extract of ketapang leaves (Terminalia catappa) is effective as a larvicidal against Ae. aegypti instar III but can cause damage to the midgut and cause death of the larvae.

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