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INDONESIA
journal of Basic Medical Veterinary
Published by Universitas Airlangga
ISSN : 23026820     EISSN : 27749959     DOI : https://dx.doi.org/10.20473
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
This journal published original articles, review articles, and case studies in Indonesian or English, in the scope of JBMV has a broad coverage of relevant topics across veterinary basic medical sciences which includes: preclinical and paraclinical disciplines like Anatomy, Physiology, Biochemistry, Pathology, Microbiology, Pharmacology and Medical Education.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 100 Documents
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PENGARUH PEMAPARAN KARBOFURAN PADA INDUK MENCIT (Mus musculus) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL ANAK MENCIT MASA LAKTASI Purnomo, Edi; Luqman, Epy Muhammad; Ratnani, Hermin; Plumeriastuti, Hani; Mafruchati, Maslichah; Dhamayanti, Yeni
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21095

Abstract

Carbofuran is a broad spectrum carbamat insecticide which is used in various farm practices. An exposure of carbofuran has been reported able to induce Reactive Oxygen Species (ROS) or free radical in kidney. Free radical caused cells damage. The present study was designed to investigate the effect of carbofuran in kidneys histopathologic of mice pup whose dam were exposed by carbofuran during the lactation period. High levels of ROS can bind to various biomolecules such as proteins, lipids and nucleic acids so that carbofuran has a negative effect on tissue functions and structures. Free radical caused changes in the pathological conditions of the tissue including the kidneys. The dams were devided into four groups of five animals each. P0 animal group was received aquadest 0,1 ml per body weight (BW), P1 animal group was treated carbofuran at the dose of 1/16 LD50 (0,3125 mg per BW), P2 animal group was treated carbofuran at the dose of 1/8 LD50 (0, 625 mg per BW), and P3 animal group was treated carbofuran at the dose of 1/4 LD50 (1,25 mg per BW). The carbofuran was exposed via oral postnatal day 1st until 9th postnatal day after delivery. At postnatal day 10th, offspring were sacrified. The kidney of mice pup was collected and processed for microscopic examination with hematoxilin-eosin staining. Microscopic observations on the histology of the mice pup kidney showed an increase in the degree of kidney damage as an increase in the dose of carbofuran given to the dams. The results showed tubular changes in the form of tubular degeneration, inflammatory cell infiltration and tubular necrosis in the kidney of mice pups.
THE EFFECT OF POLYPROPYLENE PLASTIC RESIDUE ON HEATED PALM OIL AGAINST THE HISTOPATHOLOGICAL CHANGES OF KIDNEY ON MALE RATS (Rattus norvegicus) Cahyono, Shahani Azpriyanne; Eliyani, Hana; Widiyatno, Thomas Valentinus; Plumeriastuti, Hani; Hidajati, Nove; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20415

Abstract

This research was conducted to observe the effect of polypropylene plastic residue on heated palm oil against the histopathological changes on rat's kidney. There were 18 male rats (Rattus norvegicus) that used as the examination animal and were divided into three groups and were given the mixture of heated palm oil and polypropylene plastic per oral with intubation needle. The treatment was done for five weeks. The treatment for P0 group was 84 g of palm oil, for P1 was 84 g of palm oil with 2.3 g of plastic, and for the P2 was 84 g of palm oil with 6.8 g of plastic. The histopathological changes of the kidney were observed using microscope with 400x magnification and the data was analysed using Kruskal-Wallis and continued with Mann Whitney U test. The result showed that there were significant differences between three groups of treatment (P<0.05). There were effects of polypropylene plastic residue on heated palm oil against the histopathological changes of kidney in this research such as cast, epithelial sloughing and necrosis in the mild scale.
PENGARUH PEMBERIAN EKSTRAK RUMPUT KEBAR (Biophytum petersianum Klotzsch) TERHADAP JUMLAH SEL SERTOLI MENCIT (Mus musculus) YANG DIPAPAR 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN Berlina, Cyrcilia Relita; Eliyani, Hana; Sami, Abdul; Widjiati, Widjiati; Mulyati, Sri; Anwar, Chairul
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19991

Abstract

This study  was aimed  to know  the effect of Kebar grass  extract  on amount of mice  Sertoli  cell exposed  by TCDD.  This study was  experimental   study  with completely   randomized  design.  Thirty adult  male  mice  of Mus  musculus strain Balb/C,  age 11 weeks  and  weight   25 - 30 ~were    used.  Mice were divided   into five groups,   that  were:  K- was  control  (aquadest   0)   mL); K+ was  injected  with TCDD  at  dose  of 7 µg/kg   BW;  Pl  was  injected  with TCCD  and  administrated with   Kebar   extract   (0,045 mg/  g  BW/day);   P2 was   injected   with   TCCD  and administrated  with  Kebar  extract  (0,080 mg/ g BW/day);   P3 was  injected  with TCDD and  administrated with  Kebar extract  (0,135 mg/  g BW/day).   Kebar grass extract  was  administrated in 53 days.  Mice were sacrificed  and  right  testis organs were  taken.  Then, histology   preparat  with  HE  staining   were  made  and  Sertoli cells  were  counted.    Data were   analyzed  by  One  Way  ANOV A  followed   by Duncan test  (a  =  0,05).   The result  of this  study   showed that  administration  of Kebar  grass  extract  affected  amount  of mice sertoli  cells.  K+ (2,92a±0,109) showed significance difference  (p<0,05) compared   to Pl (5,00b±0,374), P2 (7,64c±0,409)  and P3 (9,68ct±0,363).  Meanwhile, amount   of Sertoli cells of P3 at the highest  dose  did not  show  significance difference   (p>0,05)  with  K-  (10,16<l±0,829).  The conclusion of this study was  administration of Kebar  grass  extract  per  oral in 53 days  could maintain amount  of mice Sertoli cell exposed  by TCDD. 
Effect of Turmeric Flour (Curcuma domestica) and Fish Oil on Daily Egg Production and Organoleptics of Quail Eggs (Coturnix-coturnix japonica) Lovela, Aprinda Ratna; Al-Arief, Moh. Anam; Ismudiono, Ismudiono; Supranianondo, Kusnoto; Lokapirnasari, Widya Paramita; Sabdoningrum, Emy Koestanti
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28591

Abstract

The purpose of this study was to determine the effect of adding the concentration of turmeric flour and fish oil to the quail formula feed. This research is an experimental study using the factorial method. This experiment used 96 quail (Cortunix cortunix japonica), which were randomized into 6 treatments with 4 replications. Quails were divided into 6 groups and treated with different concentrations of turmeric flour and fish oil in each feed formula. The effects of different formula feeds were observed on changes in egg production and organoleptic changes. Egg production data were taken every day from week 3 to week 4 during the treatment period. The data taken is called Quail Day Production (QDP%). The organoleptic data of eggs were taken at the 3rd week of treatment and the preference test was carried out by 40 panelists on the aroma, taste and level of preference of quail eggs after being boiled. The addition of 0.3% turmeric flour significantly affected egg production but its combination with fish oil did not affect egg production. The combination of the concentration of turmeric flour and fish oil does not change the aroma of the eggs, but changes the taste of the eggs.
Pengaruh Aflatoksin 9,58 ppb dalam Pakan Terhadap Biometrik Saluran Cerna dan Berat Badan Ayam Petelur Periode Awal Pertumbuhan Pratama, Dio Putra; Tehupuring, Benjamin Chr.; Poetranto, Emmanuel Djoko; Eliyani, Hana; Soeharsono, Soeharsono; Tyasningsih, Wiwiek; Hendarti, Gracia Angelina
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28585

Abstract

The purpose of this study was to determine the effect of aflatoxin content of 9.58 ppb in feed on gastrointestinal biometrics and weight gain of laying hens in the early growth period. Thirty-six day old chicks (DOC) were used in this study and were divided into two groups P0 and P1. P0 (control group) was fed with 100% basal diet and P1 (treatment group) was fed a diet with a composition of 80% basal feed + 20% aflatoxin-containing feed. After the DOC was adapted, the first feeding containing aflatoxin was given from the 20th day to the 60th day. Data collection was carried out three times, namely the 20th, 40th, and 60th days. At the end of each period, the chickens would be euthanized to collect data on the weight and length of the digestive tract and body weight. Weight gain with time increased, but had no significant effect (p>0.05) between the control and treatment groups. Body weight continues to increase because the body's homeresis function is still running well. Observations on gastrointestinal biometrics showed significant differences (p<0.05) in oesophagus weight, proventriculus weight, ventriculus weight, jejunum weight, ileum weight and length, and cecum weight because aflatoxins have a direct effect on the digestive tract they pass. The conclusion of this study was that the provision of 20% aflatoxin-containing feed did not affect weight gain but had shown an effect on the biometrics of several gastrointestinal tracts.
Effect of Vaccination Outer Membrane Protein 52 kDa on Changes in Erythrocyte Index of Tilapia (Oreochromis niloticus) Infected by Aeromonas hydrophila Nurrahmad, Nanda Rino; Yuliani, M. Gandul Atik; Ernawati, Rahaju; Chusniati, Sri; Herupradoto, Eduardus Bimo Aksono; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28592

Abstract

The purpose of this study was to determine the effect after being vaccinated by OMP 52 kDa Aeromonas hydrophila for 1 week and then infected with Aeromonas hydrophila 10 CFU/mL for 4 days on changes in erythrocyte index. Tilapia (Oreochromis niloticus) used in this study was 10-12 cm long. There were 20 tilapia (Oreochromis niloticus) which were divided into 4 groups, consisting of 2 control groups and 2 treatment groups which were given various types of vaccine formulations. Group P0 (-) (unvaccinated and infected), Group P0 (+) (unvaccinated and infected), group P1 (vaccinated with the whole cell protein "HydroVac®" and infected), and P2 (vaccinated with Outer Membrane Protein 52 kDa and infected) by intramuscular injection. Post-treatment blood samples were collected on day 5 post-infection, collected through a caudal punctie and then analyzed using a hematology analyzer. Post-treatment outcomes led to statistically significant changes (p < 0.05). Therefore, the vaccine caused a significant change in the erythrocyte index.
Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan Prakoso, Febriyono Anggela; Adikara, Raden Tatang Santanu; Kusnoto, Kusnoto; Hidanah, Sri; Dhamayanti, Yeni; Warsito, Sunaryo Hadi
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28586

Abstract

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.
Identification of Cat (Felis catus) Blood Splatter on Cotton Fabric After Periods of Drying Using Leucomalachite Green and Takayama Reagent Charles, Vanessa Ann; Lestari, Tita Damayanti; Legowo, Djoko; Ismudiono, Ismudiono; Hidajati, Nove; Wahyuni, Retno Sri
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28593

Abstract

Blood-stain or blood splatter analysis when used properly can assist in establishing a chain of events linked to violent crimes (Bevel and Gardner, 2008). The methods used in detecting blood splatters in the field are chemical methods. Leucomalachite green is a colorimetric test which is used to test the presence of blood (Castro and Coyle, 2008). Takayama reagent is a confirmatory test for blood (Strassman, 1922). The aim of this research is to detect the blood splatter on cotton fabric after it has been dried for 1 day, 3 days and 5 days using Leucomalachite green and Takayama reagent. Cotton fabric was specifically chosen for this experiment with 3 different periods of drying. The unstained cotton fabric was cut into squares, and a blood sample was splattered on each piece. The fabrics splattered with blood were then dried for 1 day, 3 days and 5 days. The blood splatter was then tested using Leucomalachite green and Takayama reagent, and the results were noted afterwards. For the control, red food dye was dried for 1 day then tested with Leucomalachite green and Takayama reagent. The image results of the Leucomalachite green test are analyzed using ImageJ software 1.8.0_112 where the red, green and blue pixels are converted to grayscale. The image results of the Takayama test are graded based on the number and pattern of crystal. In conclusion, Leucomalachite green and Takayama reagent are able to detect cat blood splatter on the cotton fabric. Leucomalachite green produced a higher intensity/ darker colour as a result of an older sample, and the lower intensity/ lighter colour as a result of a fresher sample of the Leucomalachite green test. Takayama reagent produced a densely packed pattern of crystals as a result of an older sample, and the loosely packed pattern of crystals as a result of a fresher sample of the Takayama test.
Pengaruh Probiotik Bakteri Asam Laktat dalam Air Minum Terhadap Konversi Pakan Ayam Broiler Azizah, Nanda Kholifatul; Sarmanu, Sarmanu; Utomo, Budi; Sabdoningrum, Emy Koestanti; Lokapirnasari, Widya Paramitha; Supranianondo, Koesnoto
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28587

Abstract

This study aimed to determine the effect of probiotics of lactic acid bacteria in drinking water on feed consumption, weight gain and feed conversion ratio of broiler chickens. This research is an experimental research. The experimental animals used in this study were 28 male broiler chickens strain Cobb 700 aged 20 days. P0 was not given the addition of probiotic lactic acid bacteria, P1 was given the addition of probiotic lactic acid bacteria Lactobacillus acidophilus in 1ml/liter drinking water, P2 was given the addition of probiotic lactic acid bacteria Lactobacillus casei in drinking water 1ml/liter, P3 was given the addition of probiotic lactic acid bacteria Lactobacillus fermentum in drinking water 1ml/liter, P4 was given the addition of probiotic lactic acid bacteria Lactobacillus plantarum in drinking water 1ml/liter, P5 was given the addition of probiotic lactic acid bacteria Pediococcus in drinking water 1ml/liter, P6 was given the addition of probiotic mix lactic acid bacteria in drinking water 1ml /liter. Collecting data for the last 1 week by weighing the remaining consumption of feed given and weight gain of broiler chickens. The data obtained were analyzed using the Analysis of Variant (ANOVA) statistical method and continued with Duncan. Based on the results of the calculation of the amount of feed consumption and weight gain in broiler chickens, it showed that the administration of probiotic lactic acid bacteria 1ml/liter of water in broiler chickens could increase body weight gain, reduce feed conversion rates but did not reduce broiler feed consumption (p<0.05).
Effect of Aflatoxin Contaminated Feed for 40 Days on Anatomy and Histopathology of the Thymus of Laying Hens in the Starter Phase Christy, Rachella; Soeharsono, Soeharsono; Ismudiono, Ismudiono; Eliyani, Hana; Sarmanu, Sarmanu; Plumeriastuti, Hani
Journal of Basic Medical Veterinary Vol. 10 No. 1 (2021): Journal of Basic Medical Veterinary, June 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i1.28679

Abstract

The purpose of this study was to determine the effect of aflatoxin contaminated feed given for 40 days on the anatomy and histopathology of the thymus of laying hens in the starter phase. A total of 36 DOC laying hens were divided into two groups (P0 and P1). P0 (control group) was given 100% basal starter feed CP 521 and P1 (treatment group) was given 80% basal feed CP 524 plus 20% aflatoxin contaminated feed. Feeding contaminated with aflatoxin was carried out for 40 days, starting on the 20th to the 60th day after the DOC of laying hens was adapted for 20 days. The time of surgery in this study was carried out on the 20th, 40th, and 60th days, where at each surgery time the thymus organ was taken to collect weight data and histopathological preparations were made. The thymus weights based on time showed a significant decrease, but the intergroup thymus weights did not show a significant decrease. The decrease in thymus weight is caused by the involution process. Microscopic observations based on increased cell debris and congestion showed that P0 did not show a significant difference, but P1 showed a significant difference (p>0.05) on the 40th and 60th days. The conclusion in this study was that as much as 20% of feed contaminated with aflatoxins had not affected changes in thymus weight but had shown damage to the histopathological structure of the thymus.

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