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Rachmat Hidayat
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Bioscientia Medicina : Journal of Biomedicine and Translational Research
Published by HM Publisher
ISSN : -     EISSN : 25980580     DOI : https://doi.org/10.37275/bsm
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
This journal welcomes the submission of articles that offering a sensible transfer of basic research to applied clinical medicine. BioScientia Medicina covers the latest developments in various fields of biomedicine with special attention to : 1.Rhemumatology 2.Molecular aspect of Indonesia Traditional Herb 3.Cardiology and Cardiovascular diseases 4.Genetics 5.Immunology 6.Environmental health 7.Toxicology 8. Neurology 9. Pharmacology 10. Oncology 11. Other multidisciplinary studies related medicine. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health.
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 1,123 Documents
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Antifungi Effect of Keghenyat Leaves Fraction (Acmella uliginosa) In Vitro Study Agustina Septi Hijir; Salni Salni; Joko Marwoto; Triwani Triwani; Sri Nita; Mgs Irsan Saleh; Ziske Maritska; Nita Parisa; Evi Lusiana; Nia Savitri Tamzil; Ayeshah Augusta Rosdah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.5

Abstract

Introduction Candida is a yeast fungus classes most commonly found in the oral cavity, gastrointestinal tract, reproductive tract and skin, especially the species Candida albicans. Alternative medicines such as herbs are easily available and affordable by the community, such as keghenyat leaves (Acmella uliginosa). Aim of StudyTo determine the activity of Keghenyat leaves fraction (Acmella uliginosa) compared to Nystatin against Candida albicans in vitro. MethodsIn vitro study was held in Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Science and Medical Faculty, Sriwijaya University in December 2015 and January 2016. Research stages: extraction, fractionation, sensitivity test using Nystatin and Candida albicans, fractions antifungal activity test, the determination of minimum inhibitory concentration (MIC), bioautografi test and determination of compound classes. One final stages of testing the active fraction Keghenyat leaves (Acmella uliginosa) with Nystatin. Data were analyzed using ANOVA and Post Hoc Duncan test and linear regression using SPSS 20. ResultsHexane faction of Keghenyat leaves (Acmella uliginosa) is active against the fungus Candida albicans, has a Minimum Inhibitory Concentration (MIC) of 0.625% (6250μl) against the fungus Candida albicans antifungal contain active compounds that terpenoids and flavonoids, amounting to 84.68 mg / ml equivalent to 1 mg / ml Nystatin against the fungus Candida albicans. ConclusionThere is a significance different MIC between hexane fraction of Keghenyat leaves (Acmella uliginosa) and Nystatin, Nystatin have more high activity.
Telmisartan Prevents Myocardial Fibrosis via Decreasing Fraction of Colagen Type 1 Volume in Myocardial Tissue in Wistar Rats-Induced High Salt Intake Radiyati Umi Partan; Rachmat Hidayat; Mgs Irsan Saleh; Nita Parisa; Evi Lusiana; Nia Savitri Tamzil; Ayeshah Augusta Rosdah; Muhammad Reagen
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.6

Abstract

Myocardial fibrosis is a pathological condition that responsible for initiation of heart failure. Neurohormonal endogen, angiotensin II, has a potential role to activate endothelin I, TGF-β1, myocardial fibroblast, extracelullar matrix deposition, structural changes and decreasing of cardiac function. Fibrotic process is also influenced by PPAR γ. Telmisartan has a potential effect to inactivate angiotensinergic system and to activate PPAR γ. It is expected that telmisartan has optimal effect to protect myocardial fibrosis. To know the role of variation dose of telmisartan to decrease collagen type 1 fraction volume in cardiac tissue of Wistar rats. Ten-week-old male Wistar Rat (n = 30) were randomized into five groups, and each group consisted of 6 rats. Group 1 : negative control. Group 2 : rats were induced by intake Nacl 8% doses 2% body weight for eight weeks. Group 3 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 3 mg/kgBB for eight weeks. Group 4 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 6 mg/kgBB for eight weeks. Group 5 : rats were induced by intake Nacl 8% doses 2% body weight and telmisartan 12 mg/kgBB for eight weeks. Collagen volume fraction was assessed by immunohistochemistry and ImageJ program. ANOVA test followed pos hoc test was used to analyzed each variable. Collagen volume fraction significantly decreased in group 3, 4 and 5 compared in group 2. Telmisartan decreases collagen type 1 volume fraction of myocardial tissue .
The Efficacy of Lerek Fruits (Phrynium maximum)Extract Related Body Weight, Lipid Profile and Leptin in Wistar Rats-Induced High Fat Diet Radiyati Umi Partan; Rachmat Hidayat; Yeni Agustin; Mgs Irsan Saleh; Nita Parisa; Evi Lusiana; Nia savitri Tamzil; Ayeshah Agusta Rosdah; Abdulwahab Hakimi
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.10

Abstract

Background Phrynium maximum (local name : lerek ) is a plant native to Sumatera, Malaya and Borneo. This plants have a potential as a local wisdom for therapeutics. In Sumatera, Lerek was used as traditional treatment for fever, diarrhea, diabetics and for wound healing. Aim of Study Aim of this study to explore the efficacy of lerek fruits extract in body weight, lipid profile and leptin level in Wistar Rats-Induced High Fat Diet Methods This study was an experimental study , pre-post test with control group design. The sample in this study was 30 male rats, 8 weeks old, weight 150-200 gram. Rats were given high fat diet and lerek fruits extract at dose of 50, 100 and 200 mg/kgBW/day for 2 weeks. Lerek fruits was extracted by infusion methods. The results of this study were assayed by SPSS 18. Results Lerek fruit extract 100 mg/kgBW was more potent to reduce body weight gain, triglyseride level and leptin level than lerek fruit extract 50 mg/kgBW, 200 mg.kgBW, negative control and positive control. Conclusion Lerek fruit extracts had a potention to reduce body weight, triglyceride and leptin level. Keywords: Lerek fruit extract – body weight- triglyserida - leptin
The Efficacy of Temu Putih Fraction (Curcuma Zedoaria (Berg) Roscoe) Related Quality and Quantity of Spermatozoa in Male Wistar Rats Tiara Fatrin; Salni Salni; Sri Nita; Rachmat Hidayat; Triwani Triwani; Joko Marwoto; Ziske Maritska; Mgs Irsan Saleh; Nita Parisa; Nia savitri Tamzil; Evi Lusiana; Ayeshah Agusta Rosdah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.12

Abstract

Background Male participation in KB is still relatively low when compared to the participationof women. Researchers have to do research to find the contraception drug. Temu putih (Curcuma Zedoaria (Berg) Roscoe) is one of traditional herb that used as antifertility. Aim of Study Aim of this study to examine change in the amount, motility, morphology, and viability spermatozoa male rats (rattus norvegicus) due to temu putih fraction suplementation. Methods This study was an experimental study using a completely randomized design (CRD), post test with control group design. The sample in this study was 30 male rats, 10 weeks old, weight 150-200 gram. Rats were given temu putih fraction (n hexan, etylacetate and methanol-water) at dose of 300 mg/kgBB/day for 48 days. Temu putih was extracted by ethanol and did fractionation by liquid-liquid methods. The results of this study were assayed by SPSS 18. Results The amount, motility, morphology and viability of spermatozoa in the group of metanol fraction of water decreased compared with the control group (p= 0,000), motilitas of spermatozoa in the group of metanol water fraction decreased compared with the control group. Conclusion Temu putih fraction can reduce the amount, motility, morphology, and viability of spermatozoa in male rats. Keywords: Fraction, Temu Putih, Amount of spermatozoa, Motility of spermatozoa, Viability Of Spermatozoa
The Relationships Between Single Nucleotide Polymorphisme (rs867500) STX1A Gene Exon 10 and Intelectual Intelligence Dalinur Qur'andini; Yuwono Yuwono; Triwani Triwani
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 1 No. 1 (2017): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v1i1.13

Abstract

Background: STX1A consists of 10 exons covering a genome overlay of 20.42 kb (2064 base mRNA) . The location of HPC-1 / Syntaxin 1A is on chromosome 7q11.23. Genes STX1A or HPC-1 or Syntaxin 1A are genes that encode membrane proteins that play an important role in neurotransmitter exocytosis in nerve cells. Aim of Study: The purpose of this study was to determine the relationship SNP (rs867500) in the gene STX1A Exon 10 with level intellect. Method: This research was observational analytic with cross sectional study design with a population of 368 and a sample of 36 were divided into two groups, namely the above-average IQ and IQ group average. To determine the relationship SNP (rs867500) STX1A gene Exon 10 with a level of intelligence used X2 test (Chi-square). Results: The results showed not found SNPs in genes STX1A exon 10. Conclusion : STX1A Genes can be said to contribute to the collective intelligence of other genes, ie genes and gene IGFR2 FNBPL1 although there is no STX1A gene polymorphism in exon 10. Keywords: Gene STX1A Exon 10, polimorphism, SNP, intelectual, intelligence.
Efficacy of Topical Coffee Paste to Collagen Density in Wistar Rats Incision Wound Debby Handayati Harahap; Fifa Argentina; Yuli Kurniawati; Citra Tresna Murti; Yudha Dwi Satrio
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.14

Abstract

Background :Collagen plays a very important role in humans at every stage of wound healing. Coffee contains many antioxidants that stimulate the formation of collagen dermis by increasing the production of Tissue Inhibitor of Matrix Metalloproteinas-1 in the dermis that serves to inhibit the breakdown of collagen. This study aims to determine the efficacy of topical pasta coffee to collagen density. Methods: Experimental Laboratories Study with pre and post test controlled group design research was undertaken from September to November 2017 at Animal House and Dyatnitalis Laboratory, Palembang. 30 male white rats of wistar strain were divided into 5 groups then performed 2 cm dermal incision and giving various concentration of coffee paste. On the day 0 and 5th after coffee paste, the percentage of collagen density is checked. The efficacy of coffee paste was analyzed by Paired T Test/Wilcoxon while the comparison of efficacy was analyzed by Independent T Test / Mann Whitney then continued with One Way ANOVA Test and Tukey test. Data analysis using SPSS version 18.0. Results: There was an average difference of percentage level of collagen density before and after vitamin C (p = 0,003), coffee paste 20% (p = 0,043), coffee paste 40% (p = 0,045) and coffee paste 80% (p = 0,011) . In addition, there was an average difference of percentage of collagen density between negative control group with positive control group and 40% coffee paste. From the results of Post Hoc test results showed that there was a difference of percentage of collagen density of negative control group with coffee paste group 40%. Conclusion: There is efficacy of topical coffee paste on collagen density in various concentrations where the highest increase of collagen density is 246,96% after coffee paste 40%. Keyword: Coffee Pasta, Collagen, Injured Incision, Vitamin C
Papaya Leaf (Carica papaya L.) Active Fraction Effective as Bioinsectiside Against Anopheles species (Diptera: Culicidae) Larva Invitro Study Chairil Anwar; Febriyanto Febriyanto; Dalilah Dalilah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.15

Abstract

ABSTRACT Malaria is one of the spread-disease that become a global public health problem included Indonesia. One of the controlling approach of vector-borne disease is by avoiding direct contact of human and mosquitoes. Natural insecticide is an alternative method that can be used. Carica papaya L. is one of the natural insecticide because contains of alkaloid, flavonoid and saponin. This research was aimed to determine the activity of active fraction. was a quasi-experiment research with post test only group design. This research was conducted in April – June 2017. Extraction and fractination was performed in laboratory of MIPA Biologi of Sriwijaya University. The experiment was performed in laboratory of Entomologi Baturaja. The sample of the research was Anopheles sp. larva instar III and IV. Larvacide test used 6 different concentrations which was 0,125%, 0,25%, 0,5%, 1,0% and 2,0% with aquadest as a control. Anova test and post hoc analysis with SPSS 22 software. The result of efficacy test to Anopheles sp. larva showed that N-hexan was the fraction with highest larvacide ability that kill 95% larva at 2% concentration level. Thin layer chromatography test showed a compound of Terpenoid. Activity test of active fraction N-heksan of papaya leaf with the highest mortality rate 92,5% and the lowest 46,25%. LC50 fraction N-heksan of papaya leaf was high toxicity at 24 hours of observation. Anova test showed there was significant influence and difference of various concentration of active fraction of papaya leaf to the mortality of Anopheles sp. larva with p value < α (0,00<0,05). Key words : Anopheles sp. larva, N-hexan fraction of papaya leaf, larvacide
The Development of Prototype Polyclonal Antibody of Receptor Advanced Glycation of Endproducts (RAGE) Mgs Irsan Saleh; Nita Parisa; Ziske Maritska
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.16

Abstract

Abstract Background Receptor for advanced glycation endproducts (RAGE) is a transmembrane protein that belongs to the immunoglobulin superfamily. As its name implicates, it can bind to advanced glycation endproducts, the resulting product of non-enzymatic glycosylation, and it also has the ability to interact with multiple ligands having common motifs as a so-called multi-ligand receptor. The ligands include high-mobility group protein (B)1 (HMGB1), S-100 calcium-binding protein, amyloid-β-protein, Mac-1, and phosphatidylserine. Interaction between RAGE and its ligands activates various cellular processes, including inflammation, proliferation, apoptosis, autophagy, and migration. Methods The proccess of isolation protein of RAGE was initiated with extraction protein and purification of RAGE protein. After that, the immunization of Rats was be done to produce Anti-RAGE. The confirmation of Anti RAGE was be done by SDS PAGE and Immunobloting. Results The production of Anti RAGE was enough pure compared by Anti RAGE commercial. Anti RAGE was protein that have molecular weight around 35 kD. Conclusion The methods that used in this study effective to develop production of anti-RAGE. Keywords : Anti RAGE – Isolation – Production Antibody
Identification of Lactic Acid Bacteria Isolate From Fermentation Food Bekasam Ardesy Melizah; Syarif Husin; Syifa Alkaf
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.34

Abstract

Bekasam is a local food from South Sumatera Indonesia, which is obtained through fermentation of fish. Previous study has shown that fermented food contained Lactic Acid Bacteria (LAB) bacteria, such as Lactobacillus plantarum, Lactobacillus sp, Pediococus sp and Weissella sp. The study was conducted to identify isolate LAB from bekasam. Bekasam contained fish, rice, and salt with a certain ratio. Further isolation of isolated LAB isolate, then performed PCR for bacterial analysis. Isolate 1 showed alleged bacteria Staphylococcus sp, non pathogen, while isolate 2 leads to Lactobacillus sp. The PCR results show the suitability of the bands formed between the Lactobacillus standard and the isolates. Keywords: bacteria, lactobacillus, acid
The Status of Oxidants and Antioxidants in Children with Nephrotic Syndrome Hertanti Indah Lestari; Eka Febri Zulissetiana; Ardesy Melizah
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 2 No. 1 (2018): Bioscientia Medicina: Journal of Biomedicine and Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v2i1.35

Abstract

Background: The nephrotic syndrome (NS) resistance and relapse to treatment pose challenges in the management of NS. Several experimental studies on both animals and humans have assessed the association between NS and the balance between the oxidants and anti-oxidants. The study aims to compare the status of oxidants and anti-oxidants of NS patients between the massive proteinuria, the remission, the steroid resistance and the control groups. Methods: a cross-sectional design to assess the status of oxidants and antioxidants in children with the nephrotic syndrome. The eligible subjects were divided into four groups, the massive proteinuria group, the remission group, the steroid resistant group and the control group. The status of oxidants and anti-oxidant were evaluated with the Malondialdehyde (MDA) and the Total Antioxidant Status (TAS), respectively. Results: The highest mean MDA levels was observed in the steroid resistant group followed by the massive proteinuria group and the remission group. The mean MDA level of the proteinuria group (massive proteinuria and steroid-resistant) is higher than the remission group. The mean TAS levels in the remission group were higher than the massive proteinuria group, but the difference was not statistically significant. Moreover, the mean difference of SAT between the proteinuria group and without proteinuria was not statistically significant. Conclusions: The oxidative stress marker (MDA) was higher in the NS patients with proteinuria than the patients without proteinuria. The difference in the total anti-oxidant status in NS patients with massive proteinuria, remission and steroid resistance were not statistically significant. Keywords: Nephrotic syndrome, oxidative stress, anti oxidant

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