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Ivandini Tribidasari A.
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Makara Journal of Science
Published by Universitas Indonesia
ISSN : 23391995     EISSN : 23560851     DOI : https://doi.org/10.7454/mss
Core Subject :
Makara Journal of Science publishes original research or theoretical papers, notes, and minireviews on new knowledge and research or research applications on current issues in basic sciences, namely: Material Sciences (including: physics, biology, and chemistry); Biochemistry, Genetics, and Molecular Biology (including: microbiology, physiology, ecology, taxonomy and evolution); and Biotechnology.
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Articles 880 Documents
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Polyvinyl Alcohol–Red Cabbage Nanofibers as pH-Responsive Freshness Sensors for Advanced Food Packaging Technology Foliatini, Foliatini; Wibowo, Singgih; Rochaeni, Henny; Suhartini, Suhartini; Fachrurrazie, Fachrurrazie; Prianditya, Arzzaq Imanda; Hadriansyah, Pradnadia Putri; Siregar, Naura Athira Putri; Nurpadilah, Novi; Alfiani, Putri; Rahim, Maudi; Sriwahyuni, Endah
Makara Journal of Science Vol. 28, No. 3
Publisher : UI Scholars Hub

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Abstract

The development of innovative food packaging technologies, particularly those capable of monitoring freshness, has become increasingly important in the food industry. This research explores the development of a pH-responsive freshness sensor using polyvinyl alcohol–red cabbage (PVA/RC) nanofibers. The nanofibers are fabricated through the electrospinning technique and meticulously analyzed via scanning electron microscopy, Fourier-transform infrared (FTIR) spectroscopy, and differential scanning calorimetry (DSC). The results underscore the fine structure of the nanofiber matrix, with an average diameter of ~68 nm. FTIR analysis substantiates the presence of anthocyanin compounds from RC within the PVA/RC nanofibers, which confirms the integration of beneficial components into the nanofiber matrix. Moreover, DSC investigations reveal the outstanding thermal properties of PVA/RC, which demonstrates the resilience of the nanofibers to higher temperatures, with a melting point of ~223 °C. Notably, the PVA/RC nanofibers with a 3:1 ratio exhibit excellent thermal stability, although the color change due to pH fluctuations shifts toward transparency. This study lays down the foundation for future exploration and the potential for a diverse array of applications and material enhancements. The findings presented herein open up new opportunities for the use of PVA/RC nanofibers in the development of freshness sensors, heralding a new era in smart food packaging technology.
Antibacterial Activities and Chemical Composition of Essential Oils from Sweet Orange (Citrus sinensis), Lemon Grass (Cymbopogon citratus), and Lime (Citrus aurantifolia) Peels Omoboye, Olumide; Sotola, Toyosi; Balogun, Olaoye; Bamigbade, Ololade; Oluwole, Olusola; Odeyemi, Adebowale; Oluduro, Anthonia
Makara Journal of Science Vol. 28, No. 3
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This study investigated the chemical composition and antibacterial activities of lime Citrus aurantifolia (Cc) and lemon grass Cymbopogon citratus (Ca) essential oils (EOs). Standard methods were used to determine their minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC), and gas chromatography–mass spectrometry was used to determine their composition. Each EO and their combination showed promising results in treating test bacterial infections. Cc EO showed the largest inhibition zone diameter (43 mm) against Staphylococcus aureus and Serratia marcescens, and Ca EO showed the largest (30 mm) and smallest (12 mm) inhibition zone diameters against Bacillus stearothermophilus and S. marcescens, respectively. The combination of Cc EO and Ca EO (1:1) resulted in the largest (45 mm) and smallest (8 mm) inhibition zone diameters against Klebsiella pneumoniae and Salmonella typhimurium, respectively. The MIC of Cc EO ranged from 0.78% to 6.25%, and its MBC ranged from 3.13% to 12.50%. The MIC and MBC of combined Cc EO and Ca EO ranged from 0.78% to 6.25% and from 1.56% to 12.5%, respectively. The major components of Cc EO and Ca EO were neral (44.98%) and D-limonene (56.02%). Overall, the combination of lime and lemon grass EOs performed well compared to streptomycin, making them suitable for treating test bacterial infections.
Cellular Uptake and Computational Analysis of [131I]-Xanthine and [131I]-Hypoxanthine in Human Prostate Cancer Cell Line (LNCaP) Wongso, Hendris; Mahendra, Isa; Setiadi, Yanuar; Rattyananda, Badra Sanditya; Rizaludin, Asep; Pranisuari, Ni Made Yuktikamura Galih; Kusumaningrum, Crhisterra Ellen
Makara Journal of Science Vol. 28, No. 3
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Abstract

Potent radiolabelled compounds eligible for therapy of prostate cancer need to be developed. Hence, we developed two candidate therapeutic agents bearing the iodine-131 (131I) radionuclide, namely, [131I]-xanthine (3,7-dihydropurine-2,6-dione) and [131I]-hypoxanthine (1,9-dihydro-6H-purin-6-one). The radiolabelled compounds were subjected to a cellular uptake study, which was accomplished by incubating [131I]-xanthine and [131I]-hypoxanthine with the human prostate cancer cell line (LNCaP) for 5, 15, 30, 60, and 90 min. Results showed that the accumulation of both [131I]-xanthine and [131I]-hypoxanthine in prostate cancer cells was significantly higher than the control group (131I). [131I]-xanthine rapidly accumulated in prostate cancer cells, with the highest percentage of cellular uptake of 2.73% ± 0.40% observed at 30 min of incubation. By contrast, [131I]-hypoxanthine exhibited more efficient accumulation in prostate cancer cells, especially at 60 and 90 min of incubation, with cellular uptake values of 11.5% ± 3.14% and 11.9% ± 1.83%, respectively. Furthermore, the computational analysis showed that radioiodinated xanthine and hypoxanthine provide potential binding affinities and interaction on both androgen and prostate-specific membrane antigen receptors. Overall, this study indicates that [131I]-xanthine and [131I]-hypoxanthine can be potentially developed as therapeutic agents for prostate cancer.
Modeling of Benzimidazole Derivatives as Antimalarial Agents using QSAR Analysis Hadanu, Ruslin; Sitorus, Marham
Makara Journal of Science Vol. 28, No. 3
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Abstract

In this study, quantitative structure–activity relationship (QSAR) analysis was conducted on 20 homologous compounds of benzimidazole derivatives. The structures of the benzimidazole derivatives were optimized using the semiempirical Parameterized Model 3 method of HyperChem for Windows 8.0 to obtain 14 descriptors. Then, multiple linear regression (MLR) analysis was performed using the backward method. The results of the MLR analysis obtained four new QSAR equation models. Based on statistical criteria, model 1 was determined as the best QSAR equation model in predicting the theoretical IC50 values of the new benzimidazole derivatives. As many as 20 new compounds of benzimidazole derivatives were modeled, of which 13 new compounds (23, 24, 25, 26, 27, 28, 29, 30, 31, 37, 38, 39, and 40 compounds) were recommended for synthesis in the laboratory because these compounds of benzimidazole derivatives havethey theoretically had higher antimalarial activity than chloroquine
Morphological and Molecular Identification of Saurida spp. (Teleostei: Synodontidae) from Saudi Arabia and Indonesia Santanumurti, Muhammad Browijoyo; Suciyono, Suciyono; Abu El-Regal, Mohamed Ahmed
Makara Journal of Science Vol. 28, No. 4
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Abstract

Saurida is a unique fishery commodity because it is difficult to differentiate visually. They can be only effectively con-served once accurately identified. This study aimed to determine the morphology and molecular characteristics of Sau-rida spp. from Banyuwangi (Indonesia) and Jizan (Saudi Arabia). Meristic and morphometric analysis was performed for morphological identification, whereas COI gene analysis was performed for molecular identification. Morphological analysis was performed to identify Saurida in this study, such as S. tumbil (Jizan, Saudi Arabia) and S. micropectoralis (Banyuwangi, Indonesia). S. tumbil did not have dark brown spots on the lateral line and upper caudal fin, whereas S. micropectoralis had 6–9 spots on those parts. S. tumbil also had a larger body size, white intestines, and more dorsal fin spines, and its pectoral fin could reach the pelvic fin. This was different with S. micropectoralis, which had a smaller body size, black intestines, and fewer dorsal fin spines, and its pectoral fin was far from the pelvic fin. Molecular identi-fication showed 100% of S. tumbil for the sample from Jizan and 99.84% S. micropectoralis for the sample from Banyuwangi. Morphological and molecular characteristics can be combined for Saurida identification to avoid misi-dentification for future studies.
Kinetics Model of Cananga (Cananga Odorata) Fresh Flower Extraction using Microwave Hydrodistillation Method Darnengsih, Darnengsih; Ni’mah, Hikmatun; Mahfud, Mahfud; Kartika Fitri, Ayu Chandra
Makara Journal of Science Vol. 28, No. 4
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Abstract

Recent studies have focused on improving essential oil extraction methods by incorporating advanced technologies, such as microwave-assisted hydrodistillation (MHD). Despite these advancements, further research is necessary to fully understand how different kinetic models influence extraction efficiency. This study evaluates several kinetic models for extracting essential oil from fresh Cananga flowers using MHD, examining how varying microwave power affects extraction rates and correlates with kinetic model parameters. The extraction process involved introducing 200 grams of fresh Cananga flowers in 400 mL of distilled water and subjecting the mixture to microwave power levels of 200 W, 400 W, and 600 W. The extraction lasted for 180 minutes, with measurements periodically 20 minutes. The data ob-tained analysed using first-order, second-order, power law, hyperbolic kinetic models, and the Elovich’s equation. Re-sults showed that the hyperbolic and power law models provided the best fit for the extraction data, with high accuracy demonstrated by a strong correlation (R² = 0.9992) and low root mean square error (RMSE = 0.0042). These findings suggest that these kinetic models are highly effective for describing the MHD extraction of Cananga essential oil, offer-ing insights for optimizing the process.
Degradation and Digestion of Polypropylene, Polyethylene, and Styrofoam by Tenebrio molitor L. (Coleoptera, Tenebrionoidae) Larvae Subchan, Wachju; Rohman, Abdu; Prihatin, Jekti; Salma, Fahriyah Ulimatus; Halimah, Rohima Novianti; Jannah, Alifia Wardhatul; Rosita, Risa
Makara Journal of Science Vol. 28, No. 4
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The increasing use of disposable plastic bags is affecting environmental problems because it requires thousands of years to degrade naturally. To overcome these problems, mealworms (larvae of Tenebrio molitor L.) become an alter-native solution. They can be considered as biodegradation agents of plastic because of the presence of symbiont bacte-ria in its gut that secretes plastic-depolymerizing enzymes. Therefore, this study aimed to compare the degradation and digestion ability of T. molitor in consuming various plastic types and thickness. Two designs have also been used: first, comparing the degradation and digestion of various plastic types, and second, comparing the degradation and diges-tion of various plastic bag thicknesses. The first design consists of three types of treatments, and the control group comprised three repetitions. The control group was fed with concentrate; treatment group 1 (P1), PP plastic bags; treat-ment group 2 (P2), high-density polyethylene (HDPE) plastic bags; and treatment group 3 (P3), styrofoam. The second design encompasses two treatment types, and the control group composed of repetitions. The control group was fed with concentrate; treatment group 1 (P1), HDPE plastic bags with a thickness 0.01 mm; and treatment group 2 (P2), HDPE plastic bags with a thickness of 0.02 mm. The result indicates that in the first design, the highest degradation and digestion occurred in treatment 3 (styrofoam) with an average of 0.001267 and 0.0063 fragment/individual, respective-ly. The second design the highest degradation occurred at P1 of 0.000009609 mg/day/individual. The highest digestion occurred at P1 with a total average of 0.004568 fragment/individual.
MnSOD Gene Knockout Promotes Apoptosis in Triple-Negative Breast Cancer BT-549 Cells Through Survivin Inhibition and Caspase-3 and Caspase-9 Modulation Ghanny, Niken Rahmah; Wanandi, Septelia Inawati; Arumsari, Sekar; Paramita, Rafika Indah; Syahrani, Resda Akhra; Putri, Putu Indah Paramita Adi
Makara Journal of Science Vol. 28, No. 4
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Triple-negative breast cancer (TNBC) is the most aggressive breast cancer subtype characterized by the lack of estro-gen, progesterone, and human epidermal growth factor receptor 2 (HER2) receptors. TNBC cells are becoming more aggressive due to the high expression of manganese superoxide dismutase (MnSOD) antioxidants to suppress reactive oxygen species-induced apoptosis and promote oncogenic signaling. This study was aimed to evaluate the effects of MnSOD knockout (KO) on TNBC cell apoptosis by assessing the survivin, caspase-9, and caspase-3 expressions. BT-549 TNBC cells containing the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9-edited MnSOD gene were used to evaluate the messenger RNA (mRNA) expressions of survivin using the RT-qPCR assay. The West-ern blot assay was used to measure protein expressions of survivin, caspase-9, and caspase-3. Interactions between MnSOD and apoptosis-related proteins were simulated using computational methods based on molecular docking analysis and protein−protein interaction network. This study revealed that MnSOD KO decreased the binding affinity between MnSOD and survivin, in line with the significant reduction of survivin mRNA and protein expressions whereas the protein expressions of caspase-9 and caspase-3 increased in MnSOD KO cells. Therefore, MnSOD plays a pivotal role in BT-549 cell apoptosis by modulating survivin, caspase-9, and caspase-3 gene expressions. This study provides insights into a novel therapeutic strategy to mitigate the aggressiveness of TNBC by disrupting MnSOD gene expression. Further studies should elaborate the MnSOD signaling pathways involving closely related apoptotic proteins.
Activities of Cellulolytic, Amylolytic, and Secondary Metabolites from Thermophilic Actinomycetes Collected from the Heated Soil around Cisolok Geyser Sari, Dhian Chitra Ayu Fitria; Ningsih, Fitria; Rachmania, Mazytha Kinanti; Yokota, Akira; Sjamsuridzal, Wellyzar
Makara Journal of Science Vol. 28, No. 4
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This study investigated the antimicrobial activity, cellulolytic, and amylolytic abilities of eight thermophilic Actinomy-cetes isolated from the heated soil around the Cisolok geyser, West Java, Indonesia. Cellulolytic and amylolytic abili-ties were screened for on media by adding 1% (w/v) soluble starch and carboxymethylcellulose (CMC), respectively. Antimicrobial activity was screened for using the agar-plug diffusion method by cultivating strains on various growth media. Growth at various temperatures showed that six strains could grow up to 50 °C. Seven out of eight strains showed amylolytic ability at 40 °C and 45 °C, and only four strains did so at 50 °C. Screening for cellulolytic ability showed that seven strains could hydrolyze CMC at 40 °C, whereas seven and five strains could hydrolyze at 45 °C and 50 °C, respectively. Antibacterial activity showed that strains UICC B-110 and UICC B-118 grown on various media could inhibit the growth of Gram-positive bacteria. The results of the antifungal activity test showed that strain UICC B-118, incubated for seven and 14 days, could inhibit the growth of Candida albicans. Based on 16S rRNA gene se-quence similarity and phylogenetic analyses, strains UICC B-110 and UICC B-118 were identified as Streptomyces glaucescens and Microbispora rosea subsp. rosea, respectively. These results show that thermophilic Actinomycetes from the soil around the Cisolok geyser have the potential as a source of thermostable enzymes and new bioactive compounds with antimicrobial activity.
The Effect of Thidiazuron Treatment on the Growth of Vanda limbata Blume in vivo Ditasya Putri, Novika; Setiari, Nintya; Nurchayati, Yulita; Agung Suedy, Sri Widodo
Makara Journal of Science Vol. 28, No. 4
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Vanda limbata is one of the most popular ornamental orchids. Currently, its growth is slow, making it necessary to improve its cultivation techniques. One of the possible ways to achieve this is to use plant growth regulator (PGR) treatment. Thidiazuron (TDZ) is a cytokinin used to increase the growth of V. limbata. This study aimed to determine the effect of TDZ concentration on the growth rate of V. limbata. The study involved cultivating V. limbata on planting media and spraying it with TDZ at various concentrations. This research uses a CRD (Completely Randomized Design) single-factor, which is TDZ concentration at three concentrations (factor levels) of 0, 25, and 50 ppm. Data were analyzed using analysis of variance and Duncan’s Multiple Range Test at 5% significance level. The study ran for three months and the following response variables were measure: the number of new leaves, increase in length of old leaves, length of new leaves, increase in width of old leaves, width of new leaves, number of roots, and stomatal density. The results showed that TDZ significantly increased leaf length and the number of new leaves of V. limbata, but decreased the density of stomata. Thidiazuron at 50 ppm was the best treatment for increasing the growth of V. limbata.

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