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Jurnal Sain Veteriner

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Published by Universitas Gadjah Mada

ISSN : 012660421 EISSN : 24073733 DOI : -

Core Subject : Health,

Veterinary

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Articles 824 Documents

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Kombinasi One-step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) dan Nucleic Acid Lateral Flow (NALF) sebagai Metode Deteksi Gen env-tm Virus Jembrana Strain Tabanan 1987 Asmarani Kusumawati; Fatimah Fatimah
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Jembrana disease is an acute viral disease in Bali cattle that have short incubation period, high mortality and morbidity rate. Early diagnostic methods are needed to prevent the further spreading of this disease. In this study, we combined One-step RT-PCR and NALF methods to detect env-tm gene of Jembrana virus Tabanan 1987 strain. Viral RNA isolated from a spleen of the infected cattle was used as the template. One-step RT-PCR procedure was performed using oligoprobes labeled digoxigenin and right primer sequence that were designed using the primer3plus program based on the conserves region of env-tm gene from NCBI database. The product of one-step RT-PCR was tested using NALF method instead of electrophoresis. Positive result was shown by the appearance of dark lines on the test line of digoxigenin in NALF dipstick device.

Body Weight, Physiological Status and Volatile Fatty Acid on Kacang and Etawah Crossbreed Goat by Reduction and Refeeding of Feed Quantity Bambang Suwignyo; Panjono Panjono; Aryanto Aryanto; Sarmin Sarmin; Irkham Widiyono
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The aimed of this study was to observe the effect of feed restriction and refeeding on body weight, physiological status and blood VFA on Kacang and Etawah Crossbreed Goat. Eight male goats consisting of 4 Kacang Goats and 4 Etawah Crossbred goats were used as experiment objects in this study. Both types of goats were subjected to the same treatment (three phases). First was adaptation, feed was given gradualy until reach the intended amount. Secondly, the feed was reduced by giving as much as 1.7% dry matter of body weight for each; thirdly, both goats were fed on ad libitum (refeeding phase). The variables observed were body weight changes, physiological status and blood VFA. The data obtained were analyzed by Various Random Test using Completely Randomized Design (RAL) on 2x2 factorial patterns. Duncan Multiple Range Test was used to test treatments that significantly different. The results showed that the pattern of changes in body weight on both goats breeds were relatively similar. Physiological status (pulse, rectal and respiratory temperature) on both breed of goats were not significantly different. However, decrease in the amount of feed impacted in the decrease in body temperature, pulsus and respiration, although it was still within normal range. The same also happened on blood VFA levels. Based on the results of the study, it can be concluded that breed differences breed of goat did not affect the physiological condition of goat. Differences in the amount of feed affect the physiological conditions and the performance of goat production.

Efektifitas perlakuan awal Tretinoin 0,1% Topikal pada Proses Penyembuhan di Luka Insisi pada Mencit (Mus musculus) Iswinarno Doso Saputro; Hesty Aulia Palupi; Wiwik Misaco Yuniarti
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Tretinoin has an important role in wound healing process include improve fibroplasia and collagen synthesis, maintening humoral immunity, and neutralize steroid effect.Epithelization and fibroblast proliferation were very important event in wound healing process. The aims of this study were studying epithelization and fibroblas proliferation on incision wound healing process using pre treatment of Tretinoin 0,1% topically on mice. This research used 24 male mice, 3 month of age, with 150 -180 gram of body weight. The mice were divided into 2 groups, control group (K) and treatment group (P). The control group was a group of mice with an incision wound, while the treatment group was a group of mice with an incision wound that received a pretreatment of Tretionin of 0.1% topically. Each group was divided into 2 sub groups K4, K7, P4, P7 based on time of samples collecting. K4 and P4 were examined at day 4, while K7 and P7 were examined at day 7. Epithelialization was observed by measuring the length of the epithelial incision area, whereas fibroblast proliferation was performed by counting the number of fibroblasts in five field of view on histopathologic skin preparations processed by HE staining.In this study,pretreatment of Tretinoin 0,1% topically can increase epithelialization and number of fibroblast significantly (p<0,05).This increase may be due to 1% Tretinoin in the tissues to induce keratinocyte basal proliferation including its migration to the skin surface via retinoic acid receptors ( RARδ)Pretreatment of Tretinoin 0,1% topically can increased epithelialization and number of fibroblast on incision wound healing process of albino rats

Deteksi Johne’s Disease pada Sapi Potong di Kabupaten Kebumen Berdasarkan Uji Elisa Iip Zuliana Rachmawati; Widagdo Sri Nugroho; Rahmat Setya Adji
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Kebumen Regency have been set as the source of the beef cattle breeds by the Ministry of Agriculture of the Republic of Indonesia. As the parent stock of beef cattle, these should be free from any infectious disease. Base on Balai Besar Veterinary Wates survey at 2015, some beef cattle in some districts at Kebumen indicated Johne's Disease. This research was carried out with the epidemiologic approach to detect Johne's Disease. About 173 serum specimens were taken from 173 heads of cows who have clinical symptoms of Johne's Disease. Sera were analyzed by ELISA. Base on ELISA result, 38 from 173 serum were seropositive. These cows sample come from local ancestor and there were not Johne’s disease vaccination program in Kebumen. This research proved that Johne's Disease have occurred in this populations which dedicated for parent stock of beef cattle in the District of Kebumen. Disease control program for Johne’s disease should be undertaken in this area to prevent the disease transmition.

Distribusi neuron serotonergik pada hipokampus Lasiwen (Myotis sp) sebagai satwa liar yang berpotensi menjadi reservoir virus rabies Tri Wahyu Pangestiningsih; Ariana Ariana; Irma Padeta; Arvendi Rahma Jadi; Woro Danur Wendo
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Myotis sp is an Indonesian microchiropteran, insectivore bat that potential to be a reservoir for rabies virus. Rabies is fatal viral encephalitis and bat could act as wildlife potential reservoirs for the rabies virus and can transmit the disease to humans as the zoonotic disease. Hippocampus is one of diagnostic tissue for rabies disease and serotonergic neuron could involved in pathogenesis of rabies disease. The aim of the study is to investigate the distribution of serotonergic neurons in Myotis sp hippocampus. Five Myotis sp. were captured from wild population in Central Java, and were humanly anesthetized using ketamine and xylazin. The animals were perfused intracardially using NaCl 0.9% as the pre-rinse followed by 10% formaldehyde to fix it. The cerebrums were collected and processing the for paraffin embedding. Cerebrums were sectioning in saggital sections, 12 µm thickness serially with 120 µm intervals. The tissues were staining immunohistochemistry using antibody to serotonin (1/300; Bioss, Cat. No: bs-1126R) 2 night incubation in 4 oC temperature. The solution for blocking background, secondary antibody, avidin-biotin-peroxidae complex and chromogen using kit Starr Trek Universal HRP Detection System (Biocare Medical, Cat No: STUHRP700) and were analyzed descriptivelly. The results show that serotonergic neuros were distributed in the all area of the of Myotis sp hippocampus. In dentate gyrus neuron serotonergic (Sert-IR)s are round in shape and mostly distributed in the middle layer, few in the superficially also deeper layers. In the hippocampus , the Sert-IR neurons are pyramidal in shape and distributed in the CA1, Ca2 and CA3 areas. In subiculum, the Sert-IR neurons are pyramidal in shape, more wider distributed than in the CA1 with no differences between outer layer and deeper layer. The conclutions of this research are the serotonergic neurons are distributed in the all area of hippocampus

Evaluasi Pengobatan Trematodiasis Menggunakan Albendazol pada Sapi di Kecamatan Pakem, Sleman, Daerah Istimewa Yogyakarta Felisitas Kristiyani; Nurul Aini; Agustina Dwi Wijayanti
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

The recent method of treatment for trematodiasis in cattle (age 1-10 years) using albendazol (10% of orally suspension) has deducted to evaluate the effectiveness of this anthelmintic to eradicate the trematoda infection especially Fasciola sp. and Paramphistomum sp. in Pakem subdistrict, Sleman district, Daerah Istimewa Yogyakarta Provence. These two species of trematodes were the mostly found in Yogyakarta area according to the annualy reports of Idul Qurban slaughter day. The cows used in this study were determined from three groups of farmers society at Pakem area. The physical examination and feces samplings for worm egg identification by Parfitt and Banks method were done as early examination to all populations. The positive results of Fasciola sp. and Paramphistomum sp. eggs of cows then grouped (Group A,B, and C) and treated with albendazole at dose of 10 mg/kg of body weight. All groups treated countinously for one year with albendazole; Group A repeated every two months, group B every four months, and group C every six months, respectively. Before administered the next dosing, the feces samplings and worm eggs identification were conducted to measure the effectiveness of albendazole that had applied. The results of treatments were the finding of trematoda eggs in feces then calculated descriptively. The effectiveness of albendazol of two months repetition was 31,25-100%, every four months was 38,47-46,15% and six months repeatly was 42,86%. It concluded that the highest effectiveness of albendazol to trematodiasis in cattle was two months repetition of treatment. The evaluation of albendazole treatments of cattle infected by Fasciola sp. and Paramphistomum sp. during 12 months resulted the low effectiveness because of only 30% of population that cured from infections. Keywords: cattle, albendazole, effectiveness, Fasciola sp., Paramphistomum sp. Abstrak Penelitian metode pengobatan trematodiasis pada sapi dewasa (umur 1-10 tahun) menggunakan albendazol (sediaan suspensi oral 10%) dilakukan untuk mengetahui metode terapi yang paling efektif terhadap infestasi cacing yang sering terjadi pada ternak sapi di Indonesia, khususnya di wilayah Sleman, Daerah Istimewa Yogyakarta. Infestasi cacing trematoda terutama Fasciola sp. dan Paramphistomum sp. cukup sering ditemukan di wilayah ini, dan sering ditemukan saat pemeriksaan tahunan penyembelihan hewan pada perayaan Idul Qurban. Sampel yang digunakan adalah sapi-sapi yang dipelihara di 3 kelompok ternak ( kelompok A, B, dan C) di wilayah Kecamatan Pakem, Kabupaten Sleman. Pemeriksaan fisik dan feses untuk identifikasi telur cacing dilakukan terhadap seluruh populasi di ketiga kelompok. Pengobatan yang dilakukan adalah dengan albendazol dosis 10 mg/kg berat badan, dengan metode ulangan untuk Kelompok A setiap 2 bulan sekali, Kelompok B tiap 4 bulan sekali dan kelompok C tiap 6 bulan sekali, selama satu tahun. Pemilihan sampel untuk pengamatan efektifitas obat cacing ditentukan pada sapi yang positif terinfeksi pada pemeriksaan pertama dengan ditemukan telur Fasciola sp. dan Paramphistomum sp. menggunakan metode Parfitt and Banks. Sebelum ulangan pengobatan, dilakukan pemeriksaan feses untuk mengukur efektivitas pengobatan sebelumnya. Berdasarkan data diketahui bahwa pengobatan albendazol dengan ulangan 2 bulan memberikan efektivitas berkisar 31,25-100%, ulangan 4 bulan 38,47-46,16% dan ulangan 6 bulan menghasilkan efektivitas sebesar 42,86%. Berdasarkan data tersebut disimpulkan bahwa efektivitas pengobatan menggunakan albendazol tertinggi diperoleh dengan cara mengulang pengobatan setiap 2 bulan sekali. Evaluasi terhadap seluruh populasi sapi yang terinfeksi Fasciola sp. dan Paramphistomum sp. selama 12 bulan menunjukkan rendahnya efektivitas albendazol karena hanya mampu menjaga ternak sapi dari infeksi kurang dari 30% populasi.Kata kunci : sapi, albendazol, efektivitas, Fasciola sp., Paramphistomum sp.

The Efficacy Study of Duramectin, Oxfendazole, Piperazine, and Pyrantel pamoate Against Gastrointestinal Worms In Horses In Yogyakarta Special Region*) Yuriadi Yuriadi; Ida Tjahajati; Soedarmanto Indarjulianto; Irkham Widiyono
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

This research aimed at determining the efficacy of duramectin, oxfendazole, piperazine, and pyrantelpamoate against gastrointestinal worms in horses in Yogyakarta Special Region and Central Java. The object of research involved 40 horses diagnosed with gastrointestinal worm infection. Prior to the research, all of the horses were subjected to examination for clinical symptoms and parasitology laboratory checkup for signs of worm eggs in their feces, and they were pronounced positive for experiment animals with at least 150 eggs per gram of feces per horse. The research horses were weighed to determine the dose of worm medication to be used. The research horses are divided into four treatment groups, each group consists of ten horses. Group I was given duramectin with a dose of 0.2 mg/kg of body weight. Group II was given oxfendazole with a dose of 7.5 mg/kg of body weight, Group III was given 125 mg Piperazin treatment per kilogram of body weight and Group IV was given pyrantelpamoate with a dose of 20 mg/kg of body weight. After receiving themedication, the horses were observed to document the progress in terms of clinical symptoms and the amount and type of worm eggs in their feces every three days for four times in a row. The research data results were tabulated and were descriptive-comparatively analyzed.The research result showed varying efficacy level of worm medication for horses. Oxfendazole kills Strongylus and Parascaris worms. Duramectin kills Strongylus worms and reduce some of the Parascaris worms. Piperazineandpyrantelpamoate kills the Strongylus wormsand reduce some of the Parascaris.

Seroprevalensi Penyakit Egg Drop Syndrome pada Itik di Desa Tumbak Bayuh, Kecamatan Mengwi, Badung, Bali Gusti Ayu Yuniati Kencana; I Made Kardena; Ni Wayan Apsari Shantika Pratistha
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Egg Drop Syndrome can cause detrimental impacts on breeders due to reducing production and quality of theaffected eggs.This study aim was to determine the seroprevalece ofantibody against Egg Drop Syndrome (EDS) virus in ducks. Antibody titers examination was done from 75blood samplesof ducks thathave not been vaccinatedagainstEDS virus. The duck samples were collected from Tumbak Bayuh Village, Mengwi, Badung. Serological examination was held at the Virology Laboratory,Faculty of Veterinary Medicine, Udayana University by usingHaemagglutination Inhibition (HI) test. HI test results showed that 24 samples were positive contained antibody of the EDS,while 51 samples were negative. Range of EDS antibody titer observed was from24 to 27 HI units. This results indicates that the ducks have protective antibody titer against EDS virus. The positive serum samples were also tested using HI test against Newcastle Disease (ND) virus with negative result. It can be concluded that the ducks in Tumbak Bayuh Village, Mengwi, Badung have 32% of antibody titer against EDS virus whichmight result from being exposed by EDS virus naturally.

Pengaruh fasciolosis pada sapi bali berdasarkan pemeriksaan darah, serum glutamic pyruvic transaminase (SGPT), dan alkaline phosphatase (ALP) Amirullah Amirullah; Dradjat AS; Sriasih M; Maskur Maskur; Depamede SN; Kisworo D; Kurniasih Kurniasih
Jurnal Sain Veteriner Vol 36, No 2 (2018): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Investation of Fasciola sp. On several species of cattle in Indonesia has occurred since a long time ago. Fasciolosis caused a high economical loss such as decreasing of carcass and organs, sometimes caused death of calves. The objectives of the research were to evaluate the effect of fasciolosis on Serum Glutamic Pyruvic Transaminase (SGPT), and the obstruction of bile duct by Alkaline Phosphatase (ALP) in Bali cattle suffered from fasciolosis. Faeces of Bali cattle raised in endemic areas of fasciolosis were examinated by sedimentation test to identify eggs per gram of faeces (EPG) of Fasciola sp. The blood samples of cattle which positive to fasciolosis were use to study the hematology by autoanalyser, SGPT and ALP. The results of research showed that from 89 cattle, 28 were infected by Fasciola sp. whithin of 7-115 EPG (min-max). From 20 fasciolosis cattle were indicated that MCHC of 90%, RBC of 30%, HB of 20%, and eosinophils of 2 % of cattle were lower than that of normal standard, while lymphocytes of 60%, MCV of 40%, ALP of 10% and SGPT of 5% of cattle were higher than that of normal standard. hematological appearances of cattle suffered from fasciolosis had macrocytichypochromic anemia, eosinopenia, and lymphocytosis. The increase of SGPT may be caused byhepatic cells destruction by young liver flukes, and the increase of ALP was caused by the obstruction of bile duct by mature liver flukes.

Pelacakan eskpresi protein pada testis mencit (Mus musculus) setelah paparan esktrak etanol daun mimba (Azadirachta indica) Agung Janika Sitasiwi; Sri Isdadiyanto; Siti Muflichatun Mardiati
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Abstract Azadirachta indica (Neem) has been shown to affect the fertility of mice by interfering with the synthesis of testosterone in mice. The aim of this study was to detect the testes protein expression of mice after exposure to the ethanolic Neem leaf extract. The laboratory animals of this study were 20 male Swiss Webster mice with three months in age and body weight ranging from 27.5 grams. The mice were divided into two treatment groups, namely K (control group, exposed with distilled water) and P (treatment group, exposed to etahnolic Neem leaf extract with 14 mg/animal /day). The treated were given for 21 days and the testicular protein was carried out on the 22nd day. The variables observed were testes weight, concentration and expression of proteins isolated from the testes. The protein concentration is determined by a spectrophotometer at a wavelength of 450nm. The protein expression was observed and determined based on the results of protein electrophoresis (SDS-PAGE). The results showed that protein expression in the treatment group has a lower concentration compared to the control group. Those results was confirmed by thinner bands in SDS-PAGE result. Those proteins thought to be a fertility determinant in mammals. Keywords : anti-fertility; Neem; protein expression Abstrak Azadirachta indica (Mimba) telah terbukti mempengaruhi fertilitas mencit dengan cara mengganggu sintesis hormon testoteron pada mencit. Penelitian ini bertujuan untuk melacak ekspresi protein pada testis mencit setelah paparan ekstrak etanol daun Mimba. Hewan uji penelitian ini adalah 20 ekor mencit Swiss Webster jantan dengan umur tiga bulan dan bobot badan berkisar 27.5 gram. Hewan uji dibagi menjadi 2 kelompok perlakuan, yaitu K (kelompok kontrol, dipapar akuades) dan P (kelompok perlakuan, dipapar dengan ekstrak etanol daun Mimba dengan dosis 14 mg/ekor/hari). Pemberian bahan uji dilakukan secara oral selama 21 hari. Variabel yang diamati adalah bobot testes, konsentrasi serta eskpresi protein yang diisolasi dari testis. Isolasi protein testis dilakukan pada hari ke-22. Konsentrasi protein ditentukan dengan spectrofotometer pada panjang gelombang 450nm. Ekspresi protein diamati dan ditentukan berdasar hasil elektroforesa protein. Hasil penelitian menunjukkan bahwa ekspresi protein pada kelompok perlakuan menunjukkan konsentrasi yang lebih rendah dengan pita yang lebih tipis jika dibandingkan dengan kelompok kontrol. Kesimpulan penelitian ini adalah paparan ekstrak etanol daun Nimba menyebabkan gangguan ekspresi protein yang diduga berperan dalam menentukan fertilitas mamalia. Kata kunci : Mimba; anti-fertilitas; eskpresi protein;

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