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Jurnal Sain Veteriner
ISSN : 012660421     EISSN : 24073733     DOI : -
Core Subject : Health,
Arjuna Subject : -
Articles 824 Documents
Recombinant Human Erythropoietin: Manfaat dalam Bidang Kedokteran Yanuartono Yanuartono; Hary Purnamaningsih; Alfarisa Nururrozi; Soedarmanto Indarjulianto; Slamet Raharjo
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.77 KB) | DOI: 10.22146/jsv.48516

Abstract

Recombinant human erythropoietin (rhEPO) is one of the biotechnology-based drugs that are needed by the human medicine and has also been used in the veterinary medicine. Currently RhEPO has been widely used in the world of human medicine for the treatment of anaemia caused by renal failure, cancer, chronic inflammation and AIDS. In veterinary medicine, although there is still not much data on its achievements, rhEPO has also been used for cases of chronic renal failure in dogs and cats. However, since rhEPO is not identical to feline EPO and canine EPO, some patients eventually produce antibodies against the drug. The antibodies not only prevent EPO therapy from being effective, but also may lead to severe and life-threatening anemia. This paper provides a review of the use of RhEPO, rfEPO and rcEPO in veterinary medicine, especially for the purpose of non-regenerative anemia therapy in cases of chronic renal failure in dogs and cats. 
Menurunnya Kandungan Kolesterol Telur Itik dengan Pemberian Bawang Putih Segar dengan Waktu Analisis yang Berbeda I Nyoman Jaya; Ni Ketut Dewi Haryani; Budi Indarsih
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.551 KB) | DOI: 10.22146/jsv.48517

Abstract

The purpose of this research is to know the influence of the giving of fresh garlic against a decrease in cholesterol levels duck eggs with different analysis time. To achieve that goal, then conducted a study using 50 tail as control with local feeding without given garlic and 50 tail given 25 g/head/day of fresh garlic. The data were analyzed using T-Test, the parameters are observed in this research are cholesterol levels, triglyceride egg yolk, the consumption of feed, feed conversion, egg production, egg weight, and mortality. Analysis of cholesterol levels and triglycerides egg yolks are done at the study week I, III, and V with the method extract ether and the procedure CHOD-PAP. The results showed that there is a decrease in cholesterol 152.24 mg per dl (72.19%) and triglycerides of 130.56 mg per dl (23.42%) a very sharp from the first week to the third week. The feed consumption, egg weight, egg production, and feed conversion are not affected by the time of analysis. The results of this study indicate that to get the content of cholesterol and low fat, given fresh garlic for three weeks.  Duck Eggs; Garlic; Cholesterol
Prevalensi Strain Avian Pathogenic Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh Wahyu Prihtiyantoro; Khusnan Khusnan; Mitra Slipranata; Imron Rosyidi
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (902.827 KB) | DOI: 10.22146/jsv.48520

Abstract

Avian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. Polymerase chain reaction (PCR) was used to analyse genetopically to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. The results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. As a zoonotic agent, the isolates have an impact on human health. 
Prima Krim sebagai Solusi Penyembuh Luka pada Penderita Diabetes Meli Rahmad Dwi Ardhiansyah; Riefky Pradipta Baihaqie; Muhammad Nuriy Nuha Naufal; Muhamad Atabika Farma Nanda; Kurnia Rahmawati; Yuda Heru Fibrianto
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (7672.463 KB) | DOI: 10.22146/jsv.48521

Abstract

Number of patients with diabetes mellitus are very large, according to International Diabetes Federation (IDF) in 2013 reaches 382 billion people between the ages of 40-59 years. Diabetic has been characterized as a chronic wounds. The animal blood waste from a slaugther house is still untapped, containing PRP (Platelet Rich Plasma) that can accelerates heal of bone and soft tissue. PRP (Platelet Rich Plasma) was made from the cattle blood waste using centrifugation. The purpose of the study is to prove that the Prima cream can heal the wounds in diabetic patients. Animals used in this study is 20 Wistar rats. Induction of diabetes was using streptozootocin 65 mg/kg and nicotinamide 235 mg/kg. The animal were divided into 4 groups: non-diabetes given placebo (ND-), diabetes given placebo (DM-), diabetic rat given commercial drug were containing chloramphenicol base 20 mg + prednisolone 2.5 mg (DM+), and diabetes given cream PRP at 20% concentration (DM PRP). Parameters used to measure the rate of wound healing is wound size and histopathologic examination. The data were analyzed using Anova One Way at 95% significant rate. PRP DM group had a wound healing faster than ND- group, DM- and DM+. Based on histopathologic examination, such as epitelialization, basal membrane, fibroblasts, collagen tissue, and presence of inflammatory cells, the best curing reaction was showed in DM PRP. PRP 20% cream produces from cow blood waste can accelerate wound healing in diabetes mellitus.
Mycotoxin Binders Effect on Ovaries of Pregnant Mice Exposed to Zearalenone Ragil Angga Prastiya; Abdul Samik; Amung Logam Saputro
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6819.563 KB) | DOI: 10.22146/jsv.48522

Abstract

Mycotoxin, a secondary metabolite produced by mushroom, has proven to cause various cytotoxic effects to animals. Zearalenone is one type of mycotoxin which is produced by Fusarium graminearum mushroom and has a cytotoxic effect to the reproduction system and cattle productivity by inducing apoptosis in the animal's ovaries, uterus, and placenta. This research tested the potentials of mycotoxin binders in eliminating the effect of zearalenone which can be seen from the expression of caspase 3, the content of malondialdehyde (MDA), and the quantity of corpus luteum in the ovaries of pregnant mice (Mus musculus). Twenty mice were grouped into five different treatments, namely K+ (not exposed to zearalenone and mycotoxin binders), K- (0.1 mg/mouse/day of zearalenone dosage), P1 (0.1 mg/mouse/day of zearalenone dosage and 0.5 mg/mouse/day of mycotoxin binder dosage), P2 (0.1 mg/mouse/day of zearalenone dosage and 1 mg/mouse/day of mycotoxin binders), and P3 (0.1 mg/mouse/day of zearalenone and 2 mg/mouse/day of mycotoxin binders), with 10-days treatment time. The results showed that the potentials of mycotoxin binders could be found on the gradual decline in the expression of caspase 3 in P1, P2 and P3 compared to that in K- (negative control). The results also showed a significant difference in the content of malondialdehyde (MDA) in K- compared to that in K+, P1, P2, and P3. Meanwhile, the quantity of corpus luteum in K- was also starkly different to that in K+, P1, P2, and P3. It can then be concluded that mycotoxin binders are very effective in eliminating the exposure of zearalenone. 
Melamine, Asam Suanurat dan Melamin-Sianurat: Kaitan dengan Penyakit Saluran Perkencingan Hewan Yanuartono Yanuartono; Alfarisa Nururrozia; Soedarmanto Indarjulianto; Hary Purnamaningsih; Slamet Rahardjo
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (377.343 KB) | DOI: 10.22146/jsv.48601

Abstract

Numerous cases of acute renal failure in dogs and cats from 2004-2007 were associated with the ingestion of dog and cat pet food products and the suspected contaminant was identified as melamine and its derivatives such as cyanuric acid and melamine cyanurate. Melamine is a chemical used primarily for the manufacture of plastics, coatings, commercial filters, adhesives and compounds in the plate-making industry and kitchen appliance.But then these compounds have been abused to increase the content of nitrogen compounds in human food products and animal feed products, respectively. Increasing the content of nitrogen compounds from melamine aims to increase the value of false protein food and feed. Melamine basically has a wide security limit so it is relatively non-toxic if consumed by humans and animals. However, many studies and case reports show that melamine can cause damage to the kidneys especially if present together with cyanuric acid in the body.This paper aims to provide a simple overview and discussion in cases of damage to the urinary tract in dogs and cats that are likely caused by toxicity of melamine and its derivatives are likely to have occurred in Indonesia. 
Peluang Imbuhan Pakan Herbal-Probiotik Komersial “Promix®” Sebagai Pengganti Antibiotic Growth Promoter (AGP) pada Ayam Pedaging yang Diberi Vaksin ND Agnesia Endang Tri H. Wahyuni; Vinsa Cantya Prakasita; Thomas Emanuel Manggotu Nahak; Agustina Viktoria Tae; Jeffi Chandra; Ajiguna Ajiguna; Sruti Listra Adrenalin; Lynda Nugrahaning Imanjati; Ima Fauziah
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (301.894 KB) | DOI: 10.22146/jsv.48614

Abstract

With the prohibition of the use of Antibiotic growth promotors (AGP) used in animal feed, especially in broilers, the use of feed additives such as herbs, probiotics, prebiotics, synbiotics or mixtures of some of these ingredients is currently widely used as feed replacements for AGP substitutes. One of the commercial feed additives that contain combinations herbal and probiotics is Promix®. In broiler maintenance, one vaccine that must be given is Newcastle Disease (ND) vaccine. The purpose of this study was to determine the role of supplementation of commercial feed combination of herbs and probiotics in broilers given ND vaccines. A total of 42 broilers were divided into two, group A (vaccinated with ND) and B (not vaccinated ND), each with 21 heads. Group A is divided into groups A1, A2 and A3 each with 7 heads. The A1 group is given only basal feed; A2 basal feed and AGP; A3 basal feed and Promix®. Group B is divided by B1, B2 and B3 same as group A before. Broilers are maintained for 5 weeks and weighing each week. All data obtained were statistically analyzed using One Way ANOVA and continued with Duncan's post hoc test. The results of this study were that the group of chickens given the ND vaccine and given supplements of Promix® had greater weight gain (P˂0.05) compared to the AGP and basal groups in the fifth week while in the group of chickens that were not given the ND vaccine but given supplement Promix® feed had greater weight gain (P˂0.05) compared to the AGP and basal groups in the fourth week. In the fifth week there was no significant difference (P˂0.05) between all feed groups in the group not given the ND vaccine. The conclusion of this study that feed additives contain combinations herbal and probiotics can replace AGP as feed additive in broiler. By giving ND vaccine the weight gain of the feed group plus Promix® showed a significant increase in weight gain.
Performa Produksi dan Analisis Usaha Puyuh (Coturnix coturnix japonica) yang diberi Substitusi Black Soldier Fly Larvae (BSFL) pada Pakan Komersil Fithria Nisa Hanifah; Koesnoto Soepranianondo; Soeharsono Soeharsono; Anam Al Arif; Widya Paramita Lokapirnasari; Nenny Harijani; Siti Hadijah; Mariana Ruth Theresia Hutabarat
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.078 KB) | DOI: 10.22146/jsv.49067

Abstract

Black Soldier Fly Larvae (BSFL) is an alternative protein source for livestock. This research aimed to know the effectiveness of BSFL for commercial feed substitution, related to production, productivity, and profitability in quail farm. This study used 80 quails which divided into 4 groups and each group consist of 20 quails. The treatments contained BSFL substitution with different composition, control group (P0) was given 100% commercial feed, (P1) was given a 5% BSFL substitution, (P2) was given a 10% BSFL substitution and (P3) was given a 20% BSFL substitution. Contribution margin (CM) analysis was used to determine the yields of the groups in this study. Results showed that the highest feed consumption rate is in P3, production (egg in total and weight), productivity and yields in P3 is the highest, although there's no difference between P0, P2 and P3 based on the analysis MANOVA.  BSFL substitution at 20% is recommended to the farmers to increase profits.
Jurnal Sain Veteriner Editorial Editorial
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (19800.135 KB) | DOI: 10.22146/jsv.54812

Abstract

Aktivitas Antibiotik terhadap Biofilm Staphylococcus aureus Isolat Preputium Sapi Aceh Dewi, Maryulia; Darmawi, Darmawi; Nurliana, Nurliana; Karmil, T. Fadrial; Helmi, T. Zahrial; Fakhrurrazi, Fakhrurrazi; Erina, Erina; Abrar, Mahdi; AK, M. Daud; Admi, Masda
Jurnal Sain Veteriner Vol 38, No 1 (2020): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (468.862 KB) | DOI: 10.22146/jsv.57359

Abstract

Biofilm is one of parameters for determining pathogenic pathways of a bacterium. Bacterial virulence activities can be attenuated by antibiotics with inhibiting biofilm. The aim of this study was to examine the characteristics and activities of antibiotics against the biofilm of Stapylococcus aureus isolate which taken from Aceh cattle prepuce. The study was exploratory and experimental, using a Completely Randomized Design (RAL) factorial pattern using three types of antibiotics, three concentrations and replications. Antibiotics were tetracycline, oxytetracycline and phosphomycin with concentrations of 10%, 20% and 30% (mg/mL), respectively. Therefore, the aim of this study was to examine the characteristics and activities of antibiotics to the biofilm of Stapylococcus aureus from Aceh cattle prepuce isolate. U-bottom polystyrene 96 wells microplate and Nutrient Broth (NB) medium was used to biofilm bacterial while biofilm was stained on crystals violet 1%. Optical Density (OD) of biofilm were λ 595 nm. Data were analyzed by using Analysis of Variance (ANOVA). The results showed that the activity of Staphylococcus aureus was moderately positive, i.e 2,667cell/μL. It showed that antibiotics and concetrations have significant effect (P<0.05) to inhibit biofilm staphylococcus aureus. In additioon, tetracycline 30% (mg / mL) has the best ability to inhibit biofilms from 52,18% to 1,262 cell/μL. Based on that fact, it can be concluded that the virulence of Staphylococcus aureus was moderate positive to be false positive.

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