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Tongku Nizwan Siregar
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INDONESIA
Jurnal Kedokteran Hewan
Published by Universitas Syiah Kuala
ISSN : 1978225X     EISSN : 25025600     DOI : 10.21157
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Jurnal Kedokteran Hewan (J. Kedokt. Hewan), or Indonesian Journal of Veterinary Sciences is a scientific journal field of veterinary sciences published since 2007, published FOUR times a year in March, June, September, and December by Universitas Syiah Kuala (Syiah Kuala University) and Indonesian Veterinary Medical Association (PDHI). Jurnal Kedokteran Hewan is a double-blind review process journal that has been accredited by National Journal Accreditation (ARJUNA), with second grade (Sinta 2), Number: 200 / M / KPTS / 2020. This journal has been registered in the Indonesian Publication Index (IPI), Google Scholar, Sinta, World Cat, Directory of Open Access Journals (DOAJ), EBSCO, Copernicus, Microsoft Academic, and other scientific databases. Jurnal Kedokteran Hewan receives scientific manuscripts in veterinary sciences (veterinary miscellaneous): anatomy, histology, physiology, pharmacology, parasitology, microbiology, epidemiology, veterinary public health, pathology, reproduction, clinic veterinary, aquatic animal disease, animal science, and biotechnology.
Arjuna Subject : Kedokteran Hewan - Kedokteran Hewan (Lain-Lain)
Articles 901 Documents
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PEMBERIAN EKSTRAK VESIKULA SEMINALIS MENINGKATKAN KUALITAS SPERMATOZOA TETAPI TIDAK MEMENGARUHI KONSENTRASI SPERMATOZOA DAN TESTOSTERON TIKUS PUTIH (Rattus norvegicus) Nizwan Siregar, Tongku; Akmal, Muslim; Wahyuni, Sri; Tarigan, Hermawaty; M, Mulyadi; Nasution, Idawati
Jurnal Kedokteran Hewan Vol 8, No 2 (2014): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v8i2.2620

Abstract

Penelitian ini bertujuan mengetahui pengaruh pemberian ekstrak vesikula seminalis terhadap kualitas spermatozoa dan konsentrasi testosteron tikus putih (Rattus norvegicus). Dalam penelitian ini digunakan 20 ekor tikus putih jantan dewasa, galur Wistar, berumur 3 -4 bulan, berat badan 250-300 g dan dibagi menjadi 4 kelompok (K1, K2, K3, dan K4), masing-masing kelompok berturut-turut diberikan 0,2 ml NaCl fisiologis, 25 g cloprostenol, 0,2 ml ekstrak vesikula seminalis (EVS), dan 0,4 ml EVS secara intraperitoneal. Pada akhir perlakuan, seluruh tikus dikorbankan secara dislocatio cervicalis. Selanjutnya, kauda duktus epididimis dinekropsi untuk dikoleksi spermatozoanya. Pemeriksaan kualitas spermatozoa, meliputi motilitas dan konsentrasi spermatozoa. Pemeriksaan konsentrasi testosteron serum darah dilakukan menggunakan teknik enzymelinked immunosorbant assay (ELISA). Motilitas dan konsentrasi (x10 6 ) spermatozoa pada kelompok K1; K2; K3; dan K4 masingmasing adalah 3,000,00; 3,200,28; 2,000,86; dan 3,200,28 dan 146,6071,90; 187,8060,80; 124,2064,70; dan 129,4027,07. Konsentrasi testosteron pada kelompok K1; K2; K3; dan K4 masing-masing adalah 5,53+1,75; 4,68+4,56; 2,51+1,33; dan 2,40+1,60 ng/ml. Hasil penelitian menunjukkan bahwa pemberian EVS mampu meningkatkan motilitas spermatozoa tetapi tidak memengaruhi konsentrasi spermatozoa dan testosteron serum darah tikus putih.
THE EFFECT OF MAGNETIC FIELD ON ANTIBIOTIC INHIBITION FOR Escherichia coli AND Bacillus sp. Sumardi, Sumardi; Agustrina, Rochmah; Ekowati, Christina Nugroho; Putra, Rizani Oktanisyah; Hartono, Madi
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.8051

Abstract

This study was aimed to test the growth of bacteria Escherichia coli (E. coli) and Bacillus sp. which were exposed to magnetic fields. In the first stage of the study, the effect of magnetic fields on the growth of E. coli and Bacillus sp were observed. The futher study was aimed to evaluate the effect of magnetic field on antibiotic sensitivity againts the growth of E. coli and Bacillus sp. The magnetic fields treatments were 0.0 mT (control), 0.1 mT, 0.2 mT, 0.3 mT which were exposed for 10 hours for each treatment. Five antibiotics (trimethoprim, ampicillin, nalidixic acid, streptomycin and chloramphenicol) were used for each bacteria. The result showed that the magnetic field did not influence the colony growth of E.coli, but in Bacillus sp. was seen the increasing of colony area in magnetic field of 0.1 mT and 0.2 mT compared with control treatment. Antibiotic of trimethoprim, nalidixic acid, and ampicillin increase the growth inhibition of E. coli when the bacteria have been exposed to 0.1 mT; 0.2 mT; 0.3 mT magnetic field for 10 minutes. The inhibition by streptomycin and chloramphenicol antibiotic on E. coli did not affected by magnetic field exposure. The inhibition of Bacillus sp by trimethoprim and ampicillin increased when the bacteria have been exposed to 0.2 mT and 0.3 mT magnetic field for 10 minutes. The inhibition of nalidixic acid, streptomycin and chloramphenicol to Bacillus sp. did not affected by the magnetic field exposure.
RESPONS ANTIBODI AYAM PETELUR YANG DIBERIKAN PROTEIN EKSKRETORI/SEKRETORI DAN DITANTANG DENGAN TELUR INFEKTIF Ascaridia galli D, Darmawi; Balqis, Ummu; Tiuria, Risa; Soejoedoeno, Retno Damayanti; Pasaribu, Fachriyan Hasymi; Hambal, Muhammad; Daud, Razali
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.929

Abstract

Penelitian ini bertujuan mengetahui respons antibodi dalam serum ayam petelur terhadap ekskretori/sekretori, dan ditantang dengan telur infektif Ascaridia galli (A. galli) Sebanyak 12 ekor ayam dibagi dalam empat kelompok. Kelompok pertama adalah ayam yang tidak diimunisasi dan tidak diinfeksi (kontrol), kelompok kedua adalah ayam yang diimunisasi dengan dosis 260 g ekskretori/sekretori larva A. galli, kelompok ketiga adalah ayam yang diinfeksi dengan dosis 1000 telur infektif A. galli, dan kelompok keempat adalah ayam yang diimunisasi dengan dosis 260 g ekskretori/sekretori dan satu minggu kemudian ditantang dengan dosis 1000 telur infektif A. galli. Respons antibodi pada masing-masing kelompok dianalisis dengan uji enzymelinkedimmunosorbantassay (ELISA) setiap satu minggu selama 10 minggu pascainfeksi. Hasil penelitian menunjukkan bahwa imunisasi dan atau infeksi dapat memicu peningkatan titer antibodi serum secara signifikan (P0,05) selama 10 minggu pascainfeksi. Titer tertinggi adalah 2,631,20 OD (optical density) dicapai pada minggu ke-3 pascainfeksi dan titer terendah adalah 1,510,48 OD pada minggu ke-0. Ekskretori/sekretori dapat memicu respons antibodi serum ayam petelur terhadap A. galli.
ADDITION OF POTENTIAL PROBIOTIC BACTERIA TO INCREASE GROWTH PERFORMANCE AND FEED EFFICIENCY OF COMMON CARP (Cyprinus carpio) Yulvizar, Cut; Dewiyanti, Irma; Defira, Cut Nanda; Iqbalsyah, T.M.; Suhartono, Suhartono
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5458

Abstract

Three intestinal bacteria isolates (namely C1, C2, and C3) from common carp (Cyprinus carpio) were isolated and identified as new potential probiotics. This research aimed to study the effect of three isolates potential probiotics addition on growth performance, survival rate and feed efficiency of common carp. Completely randomized design was assigned to fourth diet treatments with five replicates. As a control, we used the feed without any supplemention of potential probiotic. The result showed that the addition of potential probiotic bacteria (C1, C2, and C3) gave significant effect on daily growth rate, survival rate and feed efficiency and could be used in aquaculture industry to reduce feeding cost.
BRIGHTNESS-MODE EKOKARDIOGRAFI DOMBA JAWA JANTAN EKOR TIPIS Fakhrul Ulum, Mokhamad; Utami, Nur Fitri; Dwi Utami, Nindya; Noviana, Deni
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2838

Abstract

Penelitian ini bertujuan mencitrakan jantung domba jawa ekor tipis (DET) secara ekokardiografi. Domba ekor tipis jantan sebanyak lima ekor dengan berat 14-16 kg berumur 10-12 bulan digunakan dalam penelitian ini. Domba dipegang dan dibaringkan pada meja khusus tanpa sedasi ataupun pembiusan. Organ jantung domba dicitrakan menggunakan ultrasonografi brightness-mode (B-mode). Transduser cembung dengan frekuensi 2,5-4 MHz digunakan untuk mencitrakan jantung pada posisi right parasternal (RPS) view dan left parasternal (LPS) viewdengan posisi long axis (LAx) dan short axis (SAx). Hasil pencitraan ekokardiografi menunjukkan bahwa bagian-bagian dari organ jantung yang tersusun atas cairan terkesan anechoic, sedangkan bagian yang tersusun atas jaringan lunak terkesan hypoechoic dengan derajat ekogenisitas yang bervariasi. Bagian-bagian struktur jantung dapat dibedakan menurut ruang jantung atrium kanan, ventrikel kanan, atrium kiri dan ventrikel kiri. Bagian-bagian jantung yang dapat dicitrakan seperti septa jantung, otot papilaris, katup jantung semilunaris, katup jantung mitralis, katup jantung trikuspidalis, pembuluh darah vena paru-paru dan aorta. Ekokardiografi B-mode pada organ jantung domba jawa ekor tipis dapat mencitrakan dengan baik struktur ruang, otot, katup dan pembuluh darah besar dengan derajat ekogenisitas yang bervariasi.
SENSITIVITY OF Microsporum canis ISOLATED FROM DOGS TOWARDS ANTIFUNGALS I Sakan, Gerson Yohanes; Indarjulianto, Soedarmanto; Nururrozi, Alfarisa; Yanuartono, Yanuartono; Raharjo, Slamet
Jurnal Kedokteran Hewan Vol 14, No 4 (2020): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i4.17617

Abstract

The dermatophytosis treatment in dogs can fail due to dermatophyte resistance to antifungals. The purpose of this study is to determine the antifungal sensitivity of dermatophyte Microsporum canis (M. canis) isolated from dogs with dermatophyte. A total of 17 M. canis dog isolateswere tested for sensitivity to 25 g fluconazole, 25 g griseofulvin, 15 g ketoconazole and 30 g terbinafine, using the disc diffusion method.The M. canis isolates were cultured on Sabouraud's Dextrose Agar media, placed an antifungal disc, and then incubated at 28 C for 7 days. Thediameter of inhibition zone was measured, then compared with the standard, to determine whether they were sensitive, intermediate or resistant.The results of this study indicate that 16 isolates were sensitive to fluconazole; 17 isolates were sensitive to griseofulvin, ketoconazole andterbinafine; and one isolate was intermediate to fluconazole. Based on these results, it is concluded that all 17 (100%) isolates are sensitive to theantifungals griseofulvin, ketoconazole, terbinafine, and 16 (94%) isolates are sensitive to fluconazole. Thus, the four antifungals can be selectedto treat dermatophytes in dogs.
APPLICATION OF ESTRUS SYNCHRONIZATION USING PGF2 AND OVULATION SYNCHRONIZATION USING hCG FOR ARTIFICIAL INSEMINATION OPTIMIZATION ON ONGOLE (PO) BREED CATTLE Mutmainnah, Mutmainnah; Amrozi, Amrozi; Tumbelaka, Ligaya ITA
Jurnal Kedokteran Hewan Vol 11, No 4 (2017): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i4.5940

Abstract

This study aimed to determine the pregnancy percentage of Ongole (PO) breed cattle by estrus synchronization and ovulation synchronization. This study used 22 cattle that were divided into three groups: Estrus synchronized cattle (K1, n= 5); ovulation synchronized heifers using ovsynch (K2, n= 6); and ovulation synchronized cow using ovsynch (K3, n= 11). Parameters measured were diameter of corpus luteum (CL) in estrus synchronization, follicular diameter upon synchronization and artificial insemination (AI), and percentage of pregnant cattle. Data obtained were statistically analyzed using analysis of variance followed by Duncan test. Results showed no significant differences (P0.05) of CL diameter at the time of estrus synchronization in all groups of cattle with an average of 16.633.79 mm. The CL diameter at the time of estrus synchronization was not significantly different among groups, with an average of 8.80 2.07 mm. Diameter of follicles during ovulation synchronization was also not significantly different among groups. The average diameter of follicles was 9.012.05 mm. Diameter of follicles at the time of estrus and ovulation synchronization was not significantly different among groups with an average diameter of follicles of 10.942.10 mm. The pregnancy percentage of K1, K2, and K3 were 60%, 16%, and 36%, respectively. There was no correlation between the diameters of follicles during estrus with the pregnancy percentage. Estrus synchronization produced higher pregnancy rate than ovulation synchronization in cow or heifers.
IDENTIFIKASI PROTEIN IMUNOGENIK TAKIZOIT DAN BRADIZOIT TOXOPLASMA GONDII STRAIN LOKAL UNTUK PENGEMBANGAN KIT DIAGNOSTIK Hanafiah, Muhammad; Aliza, Dwinna
Jurnal Kedokteran Hewan Vol 7, No 1 (2013): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i1.559

Abstract

Telah dilakukan penelitian untuk mengisolasi dan mengidentifikasi protein 28 kDa (GRA2) dari stadium takizoit dan bradizoit Toxoplasmagondii strain lokal. Cairan asites mencit galur Balb/c digunakan sebagai protein sampel melalui elektroforesis (SDS-PAGE), serum kambing untuk uji antigenitas protein 28 kDa (GRA2) dan serum darah kucing untuk melihat tingkat sensitivitas dan spesifisitas protein 28 kDa (GRA2). Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa protein 28 KDa (GRA2) telah dapat diisolasi dari stadiumtakizoit dan bradizoit Toxoplasma sp. Nilai OD405 antigenesitas ES adalah sebesar 0,578 sedangkan pada antigen GRA2 (28 kDa) diperoleh nilai OD405 adalah sebesar 0,178. Tingkat sensitivitas protein 28 kDa (GRA2) terhadap antibodi dalam serum darah kucing terinfeksi adalah sebesar 61,53% dan nilai spesifisitasnya adalah 33,33%. Tingkat sensitivitas protein ES terhadap antibodi dalam serum darah kucing terinfeksi adalah 76,92% dan tingkat spesifisitasnya adalah 33,33%.
THE DYNAMICS OF ANTIGEN SPECIFIC PROLIFERATIVE RESPONSES OF LYMPHOCYTES AT EARLY STAGES OF BOVINE PARATUBERCULOSIS INFECTION Ferasyi, T. Reza; Koets, A. P.; Stegeman, J. A.; Kruitwagen, C. L.J.J.
Jurnal Kedokteran Hewan Vol 2, No 1 (2008): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v2i1.3500

Abstract

The present study was aimed to quantify the dynamics of early antigen specific proliferative responses of lymphocytes to (protein) antigens associated with experimentalMycobacterium avium subsp. paratuberculosis (Mp) infection cattle. The data were collected from20 experimentally infected calves, and 10 uninfected control animals, during the first 2 years oftheir lives. Several purified protein derivative antigens (Ppdp, Ppda, and Ppdb), tworecombinant Mp heatshock proteins (Hsp65 and Hsp70) and whole bacteria (sonicated Mpstrain 316F) were used to measure lymphocyte proliferation in a lymphocyte proliferationassay. Data were analyzed using a linear mixed effect (LME) model. The results showedsignificant group and timed effects for all antigens tested. At several time points, the responsesin the infected group were found significantly higher as compared to control group. The Ppdantigens induced similar lymphocyte proliferation patterns, as compared to whole bacteriaantigen and Hsp70. These results indicated that the antigen specific proliferative responses oflymphocytes differs for different antigens, probably related to differences in their availabilityduring different stages of infection. The application of LME model is a useful tool for analyzingthe quantitative longitudinal datasets. Keywords: dynamics, Mp, antigen, LME
DETEKSI VIRUS AVIAN INFLUENZA SUBTIPE H5N1 DI BEBERAPA PASAR UNGGAS HIDUP DALAM WILAYAH PROVINSI JAWA BARAT SEKITARNYA Ratnawati, Atik; Dharmayanti, NLP Indi
Jurnal Kedokteran Hewan Vol 9, No 1 (2015): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i1.2778

Abstract

Pada penelitian ini dilakukan identifikasi virus avian influenza (AI) subtipe H5N1 pada unggas dan lingkungan pasar untuk mengetahui peran pasar sebagai sumber penularan virus. Metode yang digunakan dalam penelitian ini adalah pengambilan sampel swab kloaka unggas dan lingkungan di beberapa pasar di wilayah Jawa Barat dan Tangerang. Sampel selanjutnya dilakukan isolasi ribonucleic acid (RNA) dan dilakukan reverse transcriptase polymerase chain reaction (RT-PCR) dengan menggunakan primer AI subtipe H5N1. Hasil penelitian menunjukkan bahwa virus AI/H5N1 terdeteksi pada unggas dan lingkungan pasar. Disimpulkan bahwa pasar dapat menjadi sumber penularan virus AI subtipe H5N1 terhadap unggas lainnya.

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