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Organisms: Journal of Biosciences
Published by Universitas Islam Negeri Raden Intan Lampung
ISSN : 28084012     EISSN : 28084012     DOI : 10.24042/organisms
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary
Organisms is a journal issued by Biology Department Faculty of Science and Technology, Islamic State University Raden Intan Lampung, which publish twice a year, namely May and November. Organisms Journal publishes a comprehensive and essential research articles with the following scopes: Environmental science, Biodiversity, Biotechnology, Microbiology, Ecology, Physiology (animals and plants), Genetics, Human biology, Animal science, and Plant science. Organisms Journal uses the OJS System to manage the manuscript that the editorial board, editors, and peer review (blind review) can reach the real-time status of the articles.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 76 Documents
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Bioethanol Production from Ulva lactuca Macroalgae: Optimation of Acid Hydrolysis and Fermentation Time Assyifa Junitasari; Satiyarti, Rina Budi; RR. Nadya Shafa Afrida; Asep Supriadin
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/84yy9y51

Abstract

Nowadays bioethanol has been developed as a new renewable energy source of fuel because it has the same characteristics as ethanol, can increase the octane number in gasoline and be able to reduce CO2 emissions significantly. Bioethanol based on a natural ingredient of macroalgae can be used as fuel because it contains carbohydrates of glucose that can be fermented into alcohol through the process of acid hydrolysis. The study aims to identify the optimal fermentation time  and analyze the levels of bioethanol produced using GC-MS instruments from Ulva lactuca macroalgae type. The production of bioethanol was carried out using the acid hydrolysis method with H2SO4. The reducing sugar content was analyzed using the Luff-Schoorl method, followed by fermentation using Saccharomyces cerevisiae. Acid hydrolysis was conducted using 2 N H2SO4 at 80ºC for 120 minutes. Fermentation time was varied into three treatments, namely 72, 96, and 120 hours. Each treatment was performed in triplicate. The observed parameters included reducing sugar content, ethanol concentration, and bioethanol yield. Data obtained from the experiment were analyzed descriptively and presented as mean values to evaluate the effect of fermentation time on bioethanol production efficiency. The results of this study produced 15,72% reducing sugar and 0,9% bioethanol content. Accordingly, the best times of fermentation to produce bioethanol from Ulva lactuca macroalgae is for 72 hours   
Detection of Environmental DNA from Tree Bark Associated with Rubbing Behavior of Dicerorhinus sumatrensis in Way Kambas National Park Priyambodo; Zulkarnain, Danisworo; Rustiati, Elly Lestari; Kurniawati, Yeyen; Putri, Chicka Refina Rahma; Srihanto, Eko Agus; Saswiyanti, Enny; Arsan, Zulfi; Sukatmoko; Pratiwi, Dian Neli; Permatasari, Nindy
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/zv2m2645

Abstract

The development of non-invasive methods is essential for monitoring critically endangered species such as the Dicerorhinus sumatrensis. This study aimed to evaluate the potential of tree bark associated with rubbing behavior as a source of environmental DNA (eDNA) for species detection in Way Kambas National Park. A total of 30 samples were collected from tree bark surfaces showing evidence of rubbing activity. DNA was extracted and analyzed using two approaches: simple electrophoresis and polymerase chain reaction (PCR)-based molecular methods. The results showed that the simple method failed to detect visible DNA bands, indicating low DNA concentration and degradation in environmental samples. In contrast, PCR amplification successfully detected DNA in 5 out of 30 samples, including hair-derived and epithelial residues associated with rubbing behavior.containing hair residues produced more reliable detection compared to those with only epithelial traces. These findings demonstrate that tree bark able to serve as a potential non-invasive source of eDNA, particularly when analyzed using sensitive molecular techniques. This approach provides an alternative method for detecting elusive species and contributes to the development of effective conservation strategies for the sumatran rhinoceros.
Cytogenetic Evolution and Research Trends in Coffea spp.: Integrating Bibliometric Analysis with Karyotype Evidence Permatasari, Nindy; Fauziah, Lu’lu’ Kholidah; Sari, Resti Puspa Kartika; Hardani, Maisuri; ‘Aliyah, Siti Hamidatul; Siburian, Nora Vetty Vera; Saputra, Deni Tri; Nindita, Miftah Amalia; Rohidayanti, Iis; Aprilintan, Ayuhana; Syahputra, M. Damar; Kumalasari, Yuyun; Saputra, Rama Arsalta Bara; Alansyah, Rio Duta; Rahma, Chanda Rizkia; Simamora, Tomuan Harry Brossy; Rani, Zahra Fania Qud’; Priyambodo
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/e84tv628

Abstract

This study investigates cytogenetic evolution and research trends in Coffea spp. by integrating bibliometric analysis with karyotype-based evidence. Despite the rapid advancement of genomic research in Coffea spp., the integration of cytogenetic perspectives into broader research trends remains limited. Bibliometric data were retrieved from the Scopus database, covering publications from 1937 to 2026, resulting in 383 articles and reviews analyzed using Biblioshiny through thematic mapping and thematic evolution approaches. The results indicate that coffee genetic research has progressively shifted toward molecular and genomic studies, particularly those related to genetic variation, genome-wide association studies, and high-throughput analytical methods. In contrast, cytogenetic themes, including chromosome organization, karyotype variation, and polyploidization, remain comparatively underrepresented within the broader research landscape. Thematic evolution analysis further reveals a transition from foundational genetic studies to advanced genomic frameworks over time. Cytogenetic synthesis highlights major differences among key coffee species, with Coffea arabica characterized as an allotetraploid species (2n = 44), whereas C. canephora and C. liberica exhibit diploid chromosome complements (2n = 22). These findings demonstrate that chromosome-level perspectives remain insufficiently integrated into contemporary genomic research despite their importance in understanding genome evolution and species differentiation. By combining bibliometric trends with cytogenetic evidence, this study provides a more comprehensive framework for interpreting coffee genome organization and emphasizes the importance of integrating structural and molecular approaches in future coffee research and breeding programs.
Changes in Total Phenolic Content Due to Changes in Storage Temperature, Crown Pruning, and Pineapple Fruit Coating Chandra, David; Widodo, Soesiladi Esti; Kamal, Muhammad; Waluyo, Sri; Zulferiyenni, Zulferiyenni; Yosilia, Rani
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/pgm3nt91

Abstract

Phenolic compounds are substrates for the internal browning (IB) reaction in pineapple fruit, and their accumulation increases at high temperatures. However, the effect of temperature changes after prolonged cold storage on total phenolic content (TPC) remains unclear. This study evaluated the effects of storage temperature, crown pruning, and fruit coating on TPC and its correlations with IB incidence and severity, and vitamin C. GP3 and MD2 pineapple clones were arranged in a three-factor randomized block design with storage temperature (7 and 7+16 °C), crown treatment (intact crown and crown pruning), and fruit coating (50 mg L-1 ABA, 1% chitosan, ABA + chitosan, and H2O). The results showed that changing the storage temperature to room temperature for 2 days and pruning the crown at day 30 increased TPC and fruit color darkness. The interaction of changes in storage temperature and crown pruning was the treatment that produced the highest TPC. The application of fruit coating had no significant effect on TPC. The incidence and severity of IB were not affected by TPC, but were negatively correlated to the vitamin C content in pineapple. It is concluded that the presence of high enough vitamin C can suppress IB, even though the TPC content is quite high in pineapple.
The Effect of Preservatives in Cough Syrups on the Growth of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans Kartikawati, Endah; Yus Hargono Cahyaning Yudi; Henny Setiawati; Syumillah
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/8s1h2n39

Abstract

Generally, non-sterile pharmaceutical formulations contain preservatives added to protect the product from microbial growth. Preservatives are used in liquid non-sterile formulations, topical preparations, and other multi-dose forms. This study aims to assess the impact of preservatives in cough syrup on several microorganisms. The study utilized a cough syrup sample (Brand X) that was homogenized with phosphate buffer Solutions pH 7.2   and subsequently spiked with four types of microorganisms with six replicates: Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 8739, and Candida albicans ATCC 10231. Total plate count (TPC) method conducted and analyzed using one-way ANOVA with a 95% confidence level (α = 0.05), followed by post-hoc testing using SPSS 23 software. No growth of P. aeruginosa was observed in samples spiked with an initial concentration of 1.69 x 105 colonies/mL, indicating that the preservative in the cough syrup effectively inhibit P. aeruginosa. For E. coli and C. albicans, no significant difference in growth was observed compared to the initial concentration of microorganisms. S. aureus showed no significant decrease in logarithmic values, with the initial concentration of 1.88 x 105 colonies/mL reducing to 1.09 x 105 colonies/mL. The conclusion of this study is that the preservatives in cough syrup significantly reduce the growth of P. aeruginosa more effectively than S. aureus, E. coli, and C. albicans 
Assessment of Water Quality Using Macrobenthos as Bioindicators in Pier 2 Kota Agung, Tanggamus Regency Suci Wulan Pawhestri; Andandaningrum, Della; Arrumaisha Vina Yofanka
ORGANISMS: JOURNAL OF BIOSCIENCES Vol. 6 No. 1 (2026): Organisms: Journal of Biosciences
Publisher : Pusat HKI, Paten, dan Publikasi Ilmiah Universitas Islam Negeri Raden Intan Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24042/sz9vpt14

Abstract

Kota Agung Pier 2 in Tanggamus Regency is a coastal area with high port activities that may affect aquatic environmental conditions. Previous studies on macrobenthos diversity in this area are limited, highlighting the need for updated ecological assessments. This study aimed to evaluate macrobenthos diversity as a bioindicator of water quality using a quantitative descriptive method across three observation stations. At each station, five sampling points with three replicate core samples (10 cm diameter, 10 cm depth) were collected during low tide in May 2025, ensuring consistent environmental conditions. The parameters measured included macrobenthos composition, temperature, depth, water clarity, pH, and dissolved oxygen (DO). Macrobenthos identified included the families Terebellidae, Ophiuridae, Conidae, and Mytilidae. Shannon-Wiener diversity (H′) ranged from 0.29–0.35 (low), evenness (E) from 0.04–0.05, and Simpson’s dominance (C) from 0.28–0.87, reflecting environmental stress and uneven species distribution. The Family Biotic Index (FBI) ranged from 3.81–3.86, indicating good water quality with low organic pollution. Physical and chemical parameters were within the standards set by Government Regulation No. 22 of 2021. Overall, the results show that while macrobenthos communities experience environmental pressures, the water at Pier 2 Kota Agung still supports aquatic life. This study provides updated data for ecological monitoring and informs sustainable coastal management

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