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Anneke Pesik
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Rumphius Pattimura Biological Journal
Published by Universitas Pattimura
ISSN : -     EISSN : 2684804X     DOI : https://doi.org/10.30598/rumphiusv5i1y2023
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Rumphius Pattimura Biological Journal membuka kesempatan untuk mempublikasikan tulisan ilmiah dalam bentuk artikel penelitian terbaru, short communication, maupun review terhadap artikel dari berbagai bidang biologi, dan cabang ilmunya seperti biodiversitas, mikrobiologi, ekologi, zoologi, botani, genetika, molekuler, bioteknologi, biosistematika, parasitologi, perilaku, pertanian, dan kehutanan. RPBJ diterbitkan oleh Jurusan Biologi û Fakultas MIPA, Universitas Pattimura. Dalam 1 tahun RPBJ memiliki 1 Volume dan 2 Issue. Dewan editor kami berasal dari berbagai rumpun ilmu biologi yang aktif dalam publikasi ilmiah skala Nasional dan Internasional, sehingga mampu melakukan proses review dengan adil dan profesional. Artikel yang diterima akan diterbitkan sesuai dengan waktu yang ditentukan yaitu di bulan Maret dan bulan September, dan akan tersedia gratis bagi pembaca
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 88 Documents
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Determination of Kinship among Orange Cultivars (Citrus sp.) Based on Flavonoid Profiles Watuguly, Theophilus Wilhelmus; Thenu, Rettodwikart
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p069-078

Abstract

Orange (Citrus sp.) is a type of plant with a superior flavor and aroma of the fruit that satisfies many tastes in Indonesian society. As one of the exceptional fruit commodities, it has economic advantages and vast marketing. Increase public awareness of the need for a source of nutrition and the benefits generated by the orange as having antioxidant, antibacterial, antiviral, hypo-allergenic, and anticancer activity, as well as industrial raw materials such as jam, juice, syrup, and deodorizer. To measure the kinship of orange species based on flavonoid content. Exploration was carried out in a basic chemical laboratory, the parameters measured were the flavonoids by using the wavelength of maximum absorption of the UV-Vis spectrophotometry of citrus flavonoids. Furthermore, profile flavonoids with UV-Vis spectrophotometry include absorption bands of the type of flavonoid. Data analysis and results of chemical constituents in the form of a binary data matrix. After that, proceed with the analysis SIMQUAL (Similarity of Qualitative Data) to obtain a similarity matrix between phenetic cultivars of orange. The phenetic similarity matrix and the clustering analysis method (Sequential Agglomerative Hierarchical and Nested), the SAHN-UPGMA (Unweighted Pair-Group Method Arithmetic Average) program, and NTSys-PC v.2.0 were used to construct the phenetic family tree (dendrogram). The result showed that by using the UV-Vis spectrophotometry profile of flavonoids, a dendrogram is formed of two main clusters: Cluster I, which consists of sweet orange cultivars, ‘Kisar’ grapefruit, sour orange, lemon, ‘suanggi’ orange, and pomelo; Cluster II consists of limau orange and lime cultivars. Flavonoid profiles using UV-Vis spectrophotometry indicate that ‘Kisar’ grapefruit and lemon have similarity coefficient of 0.88, which means close kinship with limau and lime, which have a coefficient of 0.88, meaning close kinship with cluster II. Reagent NaOH 40%, AlCl₃ 5%, and NH₃ 25% can produce color changes that indicate the presence of flavonoid content, while using distilled water does not. The kinship test consists of 2 clusters that have a difference of 0.52 (52%). This study provides a novelty approach to determining the phylogenetic relationships among Indonesian Citrus cultivars using UV-Vis spectrophotometry of flavonoid profiles, which has not been extensively applied in local Citrus diversity studies.
Ethnopharmacy and Traditional Knowledge Study with a Family Use Value Approach in Sumberbrantas Village, Bumiaji District, Batu City, East Java Rosyid Ridho, Muhammad Ainur; Trisnawati, Indah; Nurhidayati, Tutik; Purwani, Kristanti Indah; Saptarini, Dian; Kuswytasari, Nengah Dwianita
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p088-100

Abstract

Indonesia is recognized as one of the world’s most biodiverse countries, with an estimated 17% of global plant and animal species found within its borders. This biodiversity supports a wealth of medicinal plants that have been traditionally utilized by local communities. However, rapid modernization has led to a decline in the transmission of ethnobotanical knowledge. This study investigates the ethnopharmacological practices in Sumberbrantas Village, Bumiaji District, Batu City, East Java, with a focus on the Family Use Value (FUV) approach to identify the most utilized plant families in traditional medicine. Data were collected through structured interviews with 70 respondents selected using purposive and snowball sampling techniques. The study analyzed the FUV and Fidelity Level (FL) of various plant species to determine their significance in traditional healing practices. Results indicate that the Zingiberaceae and Euphorbiaceae families have the highest FUV (0.45), highlighting their widespread use in treating various ailments. Conversely, the Oxalidaceae family exhibits the lowest FUV (0.01). The FL analysis shows that Allium cepa (shallot) has the highest fidelity level (75%), demonstrating its prominence in treating multiple conditions. This research provides critical insights into the preservation of ethnomedicinal knowledge and the sustainable use of medicinal plant resources. The findings serve as a valuable reference for conservation efforts and the development of community-based herbal medicine initiatives.
International Bibliometric Analysis of Literature on Intrauterine Insemination Septriani, Nur Indah; Paramita, Pradnya; Firdiana, Vykra Aulia; Norfita, Tia Silva; Sayidinar, Aisha
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p079-087

Abstract

Intrauterine insemination (IUI) is an artificial insemination method that employs specialized devices to introduce spermatozoa into the uterus. IUI is utilized to address challenges associated with poor reproductive outcomes and to optimize the productivity of livestock. Consequently, its application and utilization has gained global attention and is being continuously developed. Therefore, this study aimed to analyze the global literature on intrauterine insemination research over the preceding decade. Documents related to intrauterine insemination research were presented in the results of a bibliometric study indexed in the Scopus database for the period 2012–2022. On a global scale, the total number of identified documents amounted to 2,721, with an average annual production of 272 documents. Ten countries worldwide were identified as leading contributors to research publications on intrauterine insemination, including the United States with the most document production (n=643), followed by Turkey (n=175), India (n=173), China (n=161), United Kingdom (n=159), Iran (n=153), Netherlands (n=152), Canada (n=140), France (n=131), and Italy (n=129). This study of novelty comprehensive bibliometric analysis to map a decade of global research trends in intrauterine insemination across both human and animal applications. Unlike previous narrative reviews that focused on clinical or technical aspects, this research highlights global collaboration networks, publication dynamics, and emerging thematic hotspots within the IUI domain. Publications related to intrauterine insemination showed an upward trajectory from 2012 until 2021, followed by a decline in 2022. The findings from this analysis provide valuable guidance for future research in the field of intrauterine insemination.
Botany, Pharmacognosy, and Phytochemical Study of Gymnanthemum amygdalina (Delile) Sch.Bip. Leaves from Bandung, West Java, Indonesia Anindita, Reza; Tahapary, Manuela Esterlita; Nathalia, Dede Dwi; Putri, Intan Kurnia; Beandrade, Maya Uzia
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p138-147

Abstract

Gymnanthemum amygdalina (Delile) Sch.Bip. possesses antioxidant, antibacterial, anticholesterol, anticancer, and antidiabetic properties. This study aimed to determine the morphology of leaves, stems, flowers, and roots, as well as the microscopic powder characteristics, phytochemical profile, and Thin Layer Chromatography (TLC) pattern of G. amygdalina leaves. The samples were collected from Bandung City, West Java, Indonesia. Methods included morphological examination, microscopic powder analysis, phytochemical screening, and TLC. The morphological characterization of G. amygdalina showed that the leaves measured 10–22 cm in length and 2–8 cm in width, with an oblong shape, acuminate tips, acute bases, pinnate venation, serrated margins, rough surfaces, woody stems, and a taproot system. Microscopic examination revealed fragments of trichomes. Phytochemical screening indicated the presence of alkaloids, flavonoids, saponins, tannins, and terpenoids/steroids. TLC analysis showed five red spots with an average Rf value ranging from 0.06 to 0.58. In conclusion, the morphological characteristics of G. amygdalina leaves were identified, microscopic analysis revealed trichome fragments, and phytochemical screening confirmed the presence of secondary metabolites. TLC analysis yielded Rf values between 0.06 and 0.58.
Diversity of Gastropods in the Intertidal Zone of Tanjung Air Panas Beach, Tulehu Village, Central Maluku Regency, Indonesia Pattikawa, Jesaja Ajub; Hulopi, Mahriyana; Uneputty, Prulley Annette; Siahainenia, Laura; Tawainella, Ilfayeni Salsabillah
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p101-107

Abstract

Research on the diversity of gastropods in the intertidal area of ​​Tanjung Air Panas Beach, Tulehu Village, Central Maluku Regency was conducted in November 2024. Gastropod samples were collected in 60 quadrants on 5 belt transects. All individuals obtained were identified to the species level and the number of individuals was counted. The results of the study showed that gastropods in the intertidal area of ​​Tanjung Air Panas Beach, Tulehu Village consisted of 9 species belonging to 1 subclass i.e. Caenogastropoda, 3 orders, namely Caenogastropoda, Littorinimorpha and Neogastropoda, 7 families and 8 genera. Nassarius globosus had the highest occurrence frequency and was found in all transects and all quadrants (100%), followed by Conus ebraeus which was found in 6 quadrants (10%) and then Canarium microurceus which was found in 4 quadrants (6.67%). The highest density is owned by N. globosus with 2,633 ind./m2 (87.78%) then followed by C. ebraeus with 0.117 ind./m2 (3.89%) and C. microurceus with 0.100 ind./m2 (3.33%). The Shannon diversity index (H’), Simpson dominance index (D) and Shannon uniformity index (E) are 0.59, 0.77 and 0.27, respectively. Based on the three indices, it can be concluded that the gastropod community in the intertidal area of ​​Tanjung Air Panas Beach, Tulehu Village has low diversity and uniformity with high dominance.
Phytoremediation of Lead (Pb) Particulate as a Nature-Based Solution for a Healthier Environment Pambudi, Priyaji Agung; Handoko, Roni Setyo
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p108-118

Abstract

Lead (Pb) particulates are a serious environmental issue due to their impact on health and ecosystems. These pollutants stem from emissions of vehicles, industries, and mining. This study aims to explore the potential of plants as phytoremediators of Pb used a literature review with data triangulation from scientific journals and institutional reports. Content analysis, tabulation, and exploratory descriptive analysis were conducted. Lead, with an atomic number of 82 and an atomic weight of 207.20, is a hazardous metal that can cause kidney damage, hypertension, anemia, nerve damage, reduced fertility, miscarriages, and lower IQ. Pb particulates can adhere or fall on leaf surfaces and are absorbed through stomata. This process occurs because the size of Pb particulates (0.2-4 µm) allows them to be absorbed through stomatal openings (2-10 µm) when they open to take in CO2, thus allowing Pb to enter and accumulate in plant tissues. Through this physiological mechanism, plants can be used as effective solutions for absorbing Pb. Some plants with high effectiveness include Polyaltia longifolia, Swietenia macrophylla, and Bougainvillea spectabilis. Planting these species in industrial zones and roads can improve air quality and provide ecosystem benefits, such as clean air, habitat space, thermal comfort, and aesthetic value.
In Vitro and In Silico Evaluation of Antioxidant Activity of Muscle and Endoskeleton from Sepia officinalis Daniella, Katherine; Ismail, Rafly Alif; Fahrudin, Fahri; Cing, Jap Mai; Alfarabi, Muhammad
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p128-137

Abstract

Sepia officinalis, commonly known as cuttlefish, is one of the diverse marine organisms consumed for its high protein content. Amino acids, as the building blocks of proteins, can function as antioxidant compounds. The high amino acid content in cuttlefish makes it a potential natural source of antioxidants. However, the use of cuttlefish in medicine, particularly as an antioxidant source, remains limited in Indonesia. At present, cuttlefish is mainly utilized as a dietary protein source. The objective of this study was to evaluate the antioxidant activity of muscle and endoskeleton extracts from S. officinalis using the DPPH (2,2-diphenyl-1-picrylhydrazyl) assay in vitro. In addition, an in silico approach was employed to analyze the interaction of these extracts with myeloperoxidase, an oxidant-producing enzyme. The results demonstrated that both muscle and endoskeleton extracts were able to scavenge free radicals, showing DPPH inhibition ranging from 46.19% to 48.42%. The highest inhibition was observed at a concentration of 200 ppm for both extracts. Furthermore, in silico analysis revealed that tyrosine and phenylalanine were the key amino acids with antioxidant potential, as they inhibited myeloperoxidase activity and could reduce oxidant production by the enzyme. These findings highlight the potential of S. officinalis as a natural antioxidant source and support its future development as a functional food ingredient.
Utilization of Sea Cucumber (Holothuria scabra) for Nutritious and Healthy Food Consumption in the Maluku Community Tuaputty, Hasan; Leiwakabessy, Fredy; Wael, Syahran
RUMPHIUS Vol 7 No 2 (2025): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv7i2p119-127

Abstract

Types of sea cucumbers consumed by communities in the Maluku region are primarily Bohadschia bivittata, Holothuria scabra, and Pentamera calcigera, which are found in sandy substrates, hence referred to as "sandy sea cucumbers." Proximate analysis results show the highest protein content at 6.19% in Pentamera calcigera and the lowest at 5.24% in Holothuria scabra. The highest fat content is 0.71% in Bohadschia bivittata, and the lowest is 0.55% in Pentamera calcigera. The highest ash content is 3.29% in Bohadschia bivittata, and the lowest is 3.10% in Pentamera calcigera. The highest moisture content is 90.65% in Bohadschia bivittata, while Holothuria scabra has nutritional values between the other two species. A significant relationship exists between knowledge and skills in managing nutritious meal preparation, as indicated by an R² value of 0.961. This suggests that 96.1% of the community's skill in preparing nutritious and healthy meals from sandy sea cucumbers is influenced by their knowledge, with only 3.99% attributed to other factors. In other words, the higher the community's knowledge about sea cucumbers as a nutritious and healthy food source, the better their skills in preparing nutritious meals.

Page 9 of 9 | Total Record : 88

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