cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Nurul Hidayah
Contact Email
sccrlaboratory@gmail.com
Phone
+628164251646
Journal Mail Official
dr.agungptr@gmail.com
Editorial Address
Jalan Kol.R.W Sugiarto, Nongkosawit, Gunungpati, Kota Semarang, 50223, Indonesia
Location
Kota semarang,
Jawa tengah
INDONESIA
International Journal of Cell and Biomedical Science
Published by Stem Cell and Cancer Research Indonesia
ISSN : -     EISSN : 28296621     DOI : https://doi.org/10.59278/
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Journal of Cell and Biomedical Science, formerly CBS Int. Journal is an open-access, peer-reviewed journal published by Stem Cell and Cancer Research (SCCR), Indonesia. The journal publishes papers describing original findings and reviews articles in all aspects of cell, molecular biology, and biomedical research. Received manuscripts are accepted for publication only after rigorously being reviewed by independent experts in the respective fields determining the originality, validity, and conclusions.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 60 Documents
 2   3   4   5   6 
Factors Influencing the Barriers to Performing Five Daily Prayers among Muslim Inpatients at RSUP Dr. Kariadi Sofro, Muchlis Achsan Udji; Ardani, Yanuar; Sammy, Alwi
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.45

Abstract

Background: Prayer is a fundamental obligation for Muslims, performed five times daily. For hospitalized Muslim patients, maintaining regular prayer routines can be challenging due to physical limitations, hospital infrastructure barriers, lack of religious knowledge, and insufficient support from healthcare providers. Objective: This study aims to identify the barriers Muslim inpatients face in performing prayers and to propose recommendations for improving spiritual accommodations in hospital settings.Methods: A qualitative study was conducted at RSUP Dr. Kariadi using in-depth interviews and participatory observation. Thirty Muslim inpatients, along with family members and medical personnel, were involved. Data were collected through semi-structured interviews and direct observations, then analyzed using thematic analysis to identify major influencing factors.Results: Physical limitations such as pain (40%), difficulty standing (50%), and movement restrictions from medical devices (33.3%) were key barriers. Facility-related issues, including inaccessible prayer rooms (70%) and lack of prayer aids (55%), significantly impacted patients' ability to pray. Religious knowledge gaps were notable, with 60% of patients unaware of permissible prayer adjustments (rukhsah). Furthermore, 80% reported no assistance from healthcare staff in facilitating prayer. Psychological factors such as stress (30%) and fatigue (25%) also affected patients' motivation, while encouragement from family and staff (50%) positively influenced prayer practices. Conclusion: Muslim inpatients encounter multiple barriers to performing prayers during hospitalization. Structured interventions, including improving hospital facilities, providing religious education, and training healthcare providers in spiritual care, are essential to support the spiritual well-being of Muslim patients.
Effect of Typhonium flagelliforme Extract on the Viability of Colorectal Cancer Cells HCT-116 Sadikin, Nadya Audina N; Nazar, M. Ariq; Ibrahim, Sugeng
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.46

Abstract

Background: Colorectal cancer (CRC) remains a significant global health challenge, with rising incidence rates, particularly among younger individuals [1]. Conventional treatments, including chemotherapy and radiation, often cause severe side effects, necessitating the exploration of alternative therapeutic options. Typhonium flagelliforme, a medicinal plant widely used in traditional medicine, has been investigated for its cytotoxic potential against HCT-116 colorectal cancer cells. Objective: This study assessed the cytotoxic effects of T. flagelliforme extract on HCT-116 colorectal cancer cells using the MTT assay. Methods: The cytotoxicity of Typhonium flagelliforme (T. flagelliforme ) extract on HCT-116 colorectal cancer cells was assessed using the MTT assay after 24 hours of treatment with different concentrations (20–100 µg/mL). Results: The findings revealed a dose-dependent reduction in cell viability, with an IC₅₀ value of 73.47 µg/mL, indicating moderate cytotoxic activity. Higher extract concentrations (100–60 µg/mL) significantly decreased cell viability, while the lowest concentration (20 µg/mL) showed a paradoxical increase, possibly due to a hormesis effect. Conclusion: The findings revealed a dose-dependent reduction in cell viability, with an IC₅₀ value of 73.47 µg/mL, indicating moderate cytotoxic activity.
Hypoxic MSCs Secretome Modulates IL-18-Mediated Inflammatory in Type 2 Diabetes Mellitus via AP-1 Regulation Amansyah, Fajar; Alif, Iffan; Irawan, Risky Candra Satria
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.47

Abstract

Background: Chronic inflammation is central to the pathophysiology of Type 2 Diabetes Mellitus (T2DM), contributing to the progression of metabolic dysfunction characterized by hyperglycaemia and insulin resistance. This study aims to investigate the therapeutic potential of the hypoxic MSCs secretome (SH-MSCs) in reducing inflammation of a T2DM rat model. Methods: T2DM was induced in Wistar rats through a high-fat diet (HFD) followed by streptozotocin (STZ) administration. A total of 24 healthy male Wistar rats were randomly assigned to five groups: healthy control, T2DM, T2DM + metformin, T2DM + SH-MSCs. Results: SH-MSCs significantly reduced IL-18 mRNA expression, a key indicator of proinflammation, and suppressed the expression of AP-1 mRNA, a crucial proinflammatory transcription factor. Conclusion: These findings highlight the therapeutic potential of SH-MSCs as an alternative approach to alleviate inflammation in T2DM.
The Role of Nigella Sativa and Phyllanthus Urinaria L Extracts Enhance Inflammation Cytokine and Growth factor in Mesenchymal Stem Cells Conditioned Medium Ayu, Dian Respati; Irawan, Risky Candra Satria; Prawitasari, Salindri; Shindy, Meirista
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.48

Abstract

Background: Mesenchymal Stem Cells (MSCs) are cells that have the multipotent ability to undergo self-renewal, differentiate and secrete various bioactive substances, such as chemokines, proteins, microRNAs (miRNAs), growth factors, and cytokines. Conditioned medium of MSC is a medium resulting from cell culture enriched with the secretome of the cultured cells. MSC-CM treated with certain factors can increase the production of growth factors such as VEGF and PDGF, which play a role in angiogenesis and tissue repair. Modification of MSC-CM with bioactive compounds can be a promising strategy to increase the effectiveness of MSCs in medical therapy. Therefore, this study aims to examine whether these herbal extracts can modulate the production of pro-inflammatory cytokines (TNF-α, IFN-γ, IL-1β, IL-6) and growth factors (SDF-1, PDGF, VEGF) in MSC conditioned medium. Methods: This study used a pre-post research design with four treatment groups. Medium culture of MSCs treated with Nigella sativa (doses of 10 µg/mL) and Phyllanthus urinaria L (doses of 25 µg/mL), which were cultured for 24 and 48 hours. Measurement of cytokine and growth factor levels was carried out using the Enzyme-Linked Immunosorbent Assay (ELISA) method for quantitative analysis. Results: The data reveal distinct patterns in the modulation of protein levels, particularly for SDF-1, IL-1β, IL-6, TNF-α, and IFN-γ, which are critical players in inflammation and tissue regeneration. Conclusion: This study showed that the Nigella sativa and Phyllanthus urinaria L extracts in modifying conditioned medium of Mesenchymal Stem Cells is significant release of pro-inflammatory cytokines and growth factors.
Temporal Dynamics of TNF-α Expression and Cell Viability in LPS-Stimulated Peripheral Blood Mononuclear Cells Cahyani, Dini; Hidayah, Nurul; Sari, Fikriya Novita
International Journal of Cell and Biomedical Science Vol 3 No 7 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i7.49

Abstract

Background: Lipopolysaccharide (LPS), a key component of Gram-negative bacterial membranes, activates innate immune responses through Toll-like receptor 4 (TLR4) signaling in peripheral blood mononuclear cells (PBMCs). This study aimed to evaluate the temporal dynamics of TNF-α expression and cell viability in LPS-stimulated PBMCs to understand the inflammatory and cytotoxic effects of prolonged LPS exposure. Methods: Human PBMCs were treated with increasing concentrations of LPS (10, 30, and 50 ng/mL) for 4, 8, 12, and 24 hours. TNF-α mRNA expression was analyzed using quantitative PCR, while cell viability was assessed via CCK-8 assay and microscopic imaging. Results: LPS stimulation induced a robust, dose-dependent upregulation of TNF-α expression, peaking at 4 hours and gradually declining over time. Concurrently, PBMC viability remained stable up to 12 hours post-stimulation but significantly decreased at 24 hours, particularly at higher LPS concentrations (30–50 ng/mL). Microscopic analysis revealed increased cellular aggregation and morphological changes consistent with immune activation and cytotoxic stress. Conclusion:LPS triggers early TNF-α expression in PBMCs through TLR4-mediated activation of the NF-κB pathway. However, prolonged exposure to LPS results in decreased cell viability, likely due to sustained inflammatory signaling and oxidative stress. These findings provide insight into the dual-phase response of PBMCs to LPS and underscore the importance of tightly regulated inflammation in innate immunity.
Cytotoxic and Antiproliferative Effects of Gynura divaricata Ethanolic Extract on HCT-116 Colorectal Cancer Cells In Vitro Putri Rifai, Fauziah Novita; Hidayah, Nurul; Sakinah, Feby Nur; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.50

Abstract

Background: Colorectal cancer is a major cause of cancer-related morbidity and mortality worldwide. The limitations of conventional treatments such as chemotherapy and radiotherapy have driven the search for alternative therapies with fewer side effects. Objective: This study aimed to investigate the cytotoxic effects of Gynura divaricata extract on HCT-116 colorectal cancer cells and evaluate its potential as a complementary anticancer agent. Methods: Gynura divaricata was extracted using ethanol through a maceration process. HCT-116 cells were cultured and treated with various concentrations of the extract. Cell viability was measured using the MTT assay, and the IC50 value was calculated through linear regression analysis. Results: Treatment with Gynura divaricata extract resulted in a dose-dependent decrease in cell viability. The IC50 value was determined to be 62.09 µg/mL, indicating significant cytotoxic activity against HCT-116 cells. Conclusion: Gynura divaricata exhibits potential as an alternative or adjunctive therapy for colorectal cancer due to its ability to reduce cancer cell viability.
Generation of M2c Macrophages Using IL-10 Exposure In Vitro Alif, Iffan; Kurniawan, Dicky Zulfa F; Wilaksono, Bhirau; Susanto, Indra
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.52

Abstract

Background : Macrophages are essential cells of the innate immune system that highly adaptable and play vital roles in tissue homeostasis, immune regulation, and development. Among their various phenotypes, the M2c subtype—induced by IL-10 and TGF-β—is known for its regulatory functions, high phagocytic capacity, and pro-angiogenic potential. This study aimed to investigate whether M2c polarization via IL-10 stimulation occurs in a dose-dependent manner by differentiating human monocyte-derived macrophages using IL-10 at concentrations of 5, 10, and 20 ng/mL. Methods : IL-10 expression levels were assessed by ELISA and qPCR. The results demonstrated a clear dose-dependent increase in IL-10 expression across both methods. Results : ELISA measurements showed IL-10 levels increasing from a mean of 3.2±0.7 pg/mL in untreated controls to 19.34±2.3 pg/mL (5 ng/mL), 35.5±7 pg/mL (10 ng/mL), and 67.2±5.8 pg/mL (20 ng/mL). Similarly, qPCR analysis revealed a corresponding increase in IL-10 gene expression, with relative fold changes from 1±0 (untreated control) to 3.1±0.4, 6±1.3, and 10.1±1.6, respectively. Conclusions : These findings indicate that IL-10 induces M2c macrophage polarization in a dose-dependent manner, providing insight into optimized conditions for generating regulatory macrophage populations for potential therapeutic applications.
A 36-Year-Old Male with Snake Bite Grade I Manus Dextra Case Report Dewi, Ratna; Pranandari, Ayun; Ardani, Yanuar
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.54

Abstract

Background: Snakebite remains a major global public health concern, particularly in tropical regions like Indonesia. Snake envenomation may lead to local and systemic manifestations including erythema, swelling, pain, hypotension, respiratory distress, and even death. Case: A 34-year-old male presented to the Emergency Department of Esensia Main Clinic with the chief complaint of a traumatic wound on the right hand resembling a snakebite, accompanied by hand swelling, pain, nausea, and dizziness. The patient had applied a cloth bandage prior to arrival. Results: The patient was diagnosed with a grade I snakebite of the right hand (manus dextra) based on clinical history and physical examination. Management included administration of anti-snake venom serum (ASVS). Conclusion: Venomous snakebites can result in severe outcomes, including death; thus, early and appropriate management is crucial. In this case, medical treatment included administration of ASVS.
Therapeutic Potential of Mesenchymal Stem Cells and Their Secretome in Ameliorating Renal Fibrosis: A Comprehensive Narrative Review Amansyah, Fajar; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.55

Abstract

Chronic kidney disease (CKD) represents a global health challenge with limited therapeutic options, often progressing to end-stage renal disease requiring dialysis or transplantation. Renal fibrosis, characterized by excessive extracellular matrix deposition and loss of functional nephrons, constitutes the final common pathway for most progressive kidney diseases. Conventional therapies primarily target symptoms rather than underlying pathological mechanisms. Mesenchymal stem cells (MSCs) have emerged as promising candidates for regenerative therapy due to their multipotent differentiation capabilities, immunomodulatory properties, and paracrine effects. Growing evidence suggests that the therapeutic benefits of MSCs are predominantly mediated through their secretome—a complex mixture of soluble factors, extracellular vesicles, and exosomes. This narrative review comprehensively examines the current understanding of MSC-based therapies for renal fibrosis, with particular emphasis on their secretome. We explore the mechanisms of action, preclinical evidence, ongoing clinical trials, and challenges in translating MSC secretome-based therapies to clinical applications. Recent advances in secretome characterization, bioengineering approaches to enhance therapeutic efficacy, and targeted delivery strategies are also discussed. Despite promising results, several hurdles remain, including standardization of preparation protocols, identification of key therapeutic components, and optimization of delivery methods. This review highlights the transformative potential of MSC secretome in renal fibrosis treatment while acknowledging the need for further research to realize its full clinical potential.
Strategies for Controlling Diabetes Mellitus in Adolescents through a Family Approach at Puskesmas after the COVID-19 Pandemic Sira, Amaze Grace; Priskila, Loury; Wicaksono, Hendi; Simanjuntak, Mario Hendrian; Nappoe, Angela Aletha Biatrix; Julianto, Dayceline Helena
International Journal of Cell and Biomedical Science Vol 3 No 8 (2024)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v3i8.56

Abstract

Background Non-communicable diseases (NCDs) have become a burden on the health system in Indonesia in recent years. The increasing prevalence of diseases such as heart disease, diabetes mellitus, and cancer has an impact on national health financing, and people's quality of life. This study is a literature review that aims to evaluate the relationship between NCD risk factors, the impact of the COVID-19 pandemic on early detection of chronic diseases, and the role of primary health facilities and family approaches in controlling NCDs. Methods Secondary data were obtained from various scientific journals and official reports through systematic searches in Clinical Key, Google Scholar, and PubMed. Discussion The review showed that risk factors such as body mass index (BMI), unhealthy diet, low physical activity, and family history significantly contributed to the increase in NCD cases. The COVID-19 pandemic has also caused serious disruptions to preventive health services, including decreased access to early detection of Type 2 Diabetes Mellitus Programs such as Posbindu PTM and PIS-PK conducted by Puskesmas have an important role in promotive and preventive efforts, although they are still faced with various obstacles. The family approach in implementing healthy living behaviors and early detection has proven crucial in supporting the success of NCD control. Conclusion: The integration of the family approach in the national NCD control strategy needs to be strengthened to improve the effectiveness of interventions and the quality of life of the community.

Page 4 of 6 | Total Record : 60

 2   3   4   5   6 

Filter by Year

2022 2025


Reset
Filter By Issues
All Issue Vol 4 No 12 (2025) Vol 4 No 11 (2025) Vol 4 No 10 (2025) Vol 3 No 9 (2024) Vol 3 No 8 (2024) Vol 3 No 7 (2024) Vol 2 No 6 (2023) Vol 2 No 5 (2023) Vol 2 No 4 (2023) Vol 1 No 3 (2022) Vol 1 No 2 (2022) Vol 1 No 1 (2022) More Issue