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International Journal of Cell and Biomedical Science
Published by Stem Cell and Cancer Research Indonesia
ISSN : -     EISSN : 28296621     DOI : https://doi.org/10.59278/
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
International Journal of Cell and Biomedical Science, formerly CBS Int. Journal is an open-access, peer-reviewed journal published by Stem Cell and Cancer Research (SCCR), Indonesia. The journal publishes papers describing original findings and reviews articles in all aspects of cell, molecular biology, and biomedical research. Received manuscripts are accepted for publication only after rigorously being reviewed by independent experts in the respective fields determining the originality, validity, and conclusions.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 55 Documents
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The Potential of Moringa Leaf Extract to Prevent Aging Targeted Cellular Senescence Sri Andriyani; Rujitoningtyas, Karimatussholikah; Wigati, Hilmi U.; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.21

Abstract

High exposure to ultraviolet light and chemicals causes cell senescence due to the overproduction of reactive oxygen species (ROS). Cell senescence is known as one of the causes of degenerative diseases. Reducing ROS levels using antioxidant compounds can overcome cell senescence. This study aimed to evaluate the potential of Moringa leaf extract (EDK) as an anti-aging agent and predict the molecular mechanisms underlying its activity. EDK active compounds were identified through qualitative phytochemical screening, a cytotoxic assay was evaluate using MTT assay, ROS level calculate under DCFDA flow cytometry assay, and cell senescence analyze using SA-β-galactosidase assay. The molecular mechanism that plays an important role in senescence was analyzed through bioinformatics studies and molecular docking in silico to investigate the interaction strength of the active compound EDK with the target protein. The results showed that the EDK ethanol extract contained flavonoid, phenolic, alkaloid, saponin, and steroid compounds. This extract did not give any cytotoxic effect on NIH3T3 cells, as evidenced by the IC50 value of 420μg/mL. EDK concentrations of ¼IC50 (105μg/mL) and ½IC50 (210μg/mL) significantly reduced ROS level in dose-dependent manner in the NIH3T3 cell population with high oxidative stress induced by Doxorubicin 10nM (Dox). The percentage of senescence cells also decreased due to EDK 210μg/mL administration up to 47.60% compared to the positive aging control Dox 10nM (78.90%). Bioinformatics studies found that p21 and TP53 proteins were most directly affected by EDK active compounds in the senescence pathway. The quercetin compound in EDK has the most robust interaction strength against p21 and TP53 proteins compared to ligands. In conclusion, it can be concluded that the active compound EDK can suppress intracellular ROS levels and compete with p21 and TP53 proteins in preventing cell senescence.
The Effect of Hypoxic Mesenchymal Cell Secretome Administration on VEGF Levels In Type 1 Diabetes Rats Model Jumena, Finanda; Amalina, Nur Dina; Dewi Antari, Arini
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.22

Abstract

Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterised by the cessation of insulin production due to pancreatic β-cell damage resulting in an increase in blood glucose. This study aims to analyse the effect of hypoxic secretome MSCs on the angiogenesis process through the observation of VEGF levels in T1DM rats model. The twenty animal model were randomly assigned to four groups: control T1DM, T1DM with HS-MSCs 0.5 mL intraperitoneal treatment (T1), and TIDM with hypoxic secretom mesenchymal stem cells (HS-MSCs) 1 mL intraperitoneal treatment (T2). The T1DM rats model was induced by a single intraperitoneal (IP) injection of freshly prepared streptozotocin (STZ) at a dose of 65 mg/kg of body weight. The VEGF levels was analyses under ELISA assay. The results showed that VEGF levels of T1 (68.86±4.78) and T2 (53.83±10.86) groups were significantly upregulated in treatment of HS-MSCs. Taken together, HS-MSCs potentially reduce glucose levels on T1DM through VEGF up-regulation.
Regulatory Effect of Secretome-Hypoxia Preconditioned Mesenchymal Stem Cells on TNF-α Level in Streptozotocin-induced Rats Amellia, Nazzala Luthfin; Antari, Arini Dewi; Nazar, Mohammad Ariq; Husain, Sofian Azalia; Arifin, Naufal Ardjivani
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.23

Abstract

Background: Type 1 Diabetic Mellitus (T1DM) is a well-known autoimmune disease characterized by a specific adaptative immunity against β-cell antigens. Mesenchymal stem cells (MSC) have emerged as potential immunomodulators in a paracrine manner via their bioactive soluble molecules that involve inflammation-related diseases, including T1DM. Objective: This study aims to investigate the effect of SHMSCs on regulating TNF-α concentrations in STZ-induced rats. Materials and Methods: This study uses a post-only control group design and randomized system that was conducted from December 2022 until January 2023. To induce T1DM-like rats, an intraperitoneal injection (65 mg/kg BW) of streptozotocin (STZ) was inducted. 15 male Wistar rats were subdivided into the following groups: STZ, STZ with 0.5 cc SHMSCs (Low-dose), and STZ with 1 cc SHMSCs (High-dose). The animals received an intraperitoneal injection of SHMSCs once a week for up to 4 weeks. On day 28, the animals were terminated and ELISA measured TNF-α concentrations. Results: After SHMSCs administration, the level of TNF-α in the treated group was decreased in either low-dose or high-dose groups compared with the STZ group. Conclusion: Administration of secretome-hypoxia MSCs may regulate TNF-α concentrations in STZ-induced Rats.
The Effect of Hypoxia on the Soluble Molecules of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Widyatmoko, Agus; Alif, Iffan; Irawan, Risky Candra Satria; Handoyo, Frigi Eko; Sidiq, Husni Ahmad
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.24

Abstract

Background: Umbilical cord-derived stem cells (UC-MSCs) are essential cell sources for cell-based therapies in regenerative medicine. Hypoxia is a key element of the stem cell niche and crucial for the preservation of UC-MSCs properties. The normal growth conditions for UC-MSCs are under atmospheric oxygen concentrations of 20–21%. However, the administration of UC-MSCs in inflammatory conditions provides oxygen-deficient environments. Thus, treating UC-MSCs with low oxygen exposure provides them with more survival and recovery potential. Objective: In this study, we assessed the impact of hypoxia incubation for 12 h on the UC-MSCs proteome. Methods: UC-MSCs were isolated from UC patients regarding informed consent. At passage 5, in 80% confluent, UC-MSCs were incubated in 5% O2 for 12 h. The morphology of UC-MSCs was assessed using a microscope. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were analyzed using ELISA. Results: Hypoxic condition could change their morphology and enhance the cellular density compared to normoxic conditions in vitro. The level of FGF-2, FGF-8, TNF-α, and HSP-70 were significantly increased after the hypoxic condition of UC-MSCs compared to normoxia. Conclusion: Our findings suggest that the hypoxic condition was able to induce survival capacity and soluble molecules secreted by UC-MSCs.
Polymeric Chitosan Gel Modulates Inflammatory Responses to Prevent Peritoneal Adhesion in Rats Makarim, Fadhli Rizal; Ekasaputra, Vito Mahendra; Arnandha, Defan Adlill; Sahardin, Renaldy; Khayin, Iqbal Yusuf
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.25

Abstract

Peritoneal adhesions are a pathological response to injury that connects adjacent structures that cause by inflammatory response such as IL-1 and IL-6. The current strategies to minimize or prevent peritoneal adhesions are very limited. Chitosan was found have antiinflammatory effect. However, chitosan hydropphobic properties give poor solubility in physiological solvent such as peritoneal fluid. Our study aims to investigate the effect of polymeric chitosan gel in the prevention of peritoneal adhesion through modulating IL-1 and IL-6 after laparotomy. Ten rats were divided into a control group and treated group. Laparotomy model was done with proper procedure. Illeum were scratch using cytobrush to mimic peritoneal adhesions. In the treated group, the defect was covered with Mediclore®. After 14 days, IL-1 and IL-6 were analyzed using ELISA from peritoneal fluid serum and macroscopic observation of adhesion were made. All animals in control group showed high grade of adhesin while treated group showed a filmy peritoneal adhesion. Group treated with Mediclore® showed a lower IL-1 levels compared to control group. Treated group showed a better control at inflammation by scoring lower IL-6 levels compared to control group. In conclusion, polymeric chitosan gel prevents peritoneal adhesions through modulation of inflammatory cytokines after laparotomy in rats. Keywords: chitosan, IL-1, IL-6, peritoneal adhesions, laparotomy
The Effect of X-Ray Radiation to IL-10 Levels in Secretome Mesenchymal Stem Cells Cosmeceutical Product Antari, Arini Dewi; Ghaissani, Shabrina Syifa; Prawitasari, Salindri; Haryono, Erlina; Adityani, Resanti
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i4.27

Abstract

Background: X-ray radiation has been widely used in the pharmaceutical industry because it regenerates and repairs damaged tissues. Objective: In this study, we evaluate the effect of X-ray radiation on the secretome cosmeutical product. Methods: We conducted interleukin 10 (IL-10) analysis by ELISA in each product sample after exposure to X-ray radiation. Results: The levels of IL-10 in each sample were significantly lower than those in the control samples. Moreover, the level of IL-10 in the product samples was significantly higher than that of the control sample. Conclusion: In conclusion, exposure to radiation during shipping or storage of skin care products can potentially damage the proteins in the products by inducing the production of reactive oxygen species (ROS) and decreasing the treatment effectiveness.
Intracavernous Stem Cell Injection for Erectile Dysfunction: Safety and Efficacy Review Lubis, Ahmad Sulaiman; Makarim, Fadhli Rizal; Aldiosta, Pradipta Hadyan
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i4.28

Abstract

Background: Erectile dysfunction is a common condition in men characterized by the inability to maintain an erectile state. Current treatment using phosphodiesterase-5 inhibitors treatment may leave significant side effects and effectivity problems in some patients. The use of stem cell transplantation in erectile dysfunction has been emerging as a replacement therapy for erectile dysfunction. The effectiveness of stem cell therapy for the treatment of erectile dysfunction has been investigated in several animal studies and one clinical trial in humans. The intracavernous injection is one of the methods to deliver stem cell treatment for erectile dysfunction besides intravenous and intraperitoneal. It was believed to improve erectile function by promoting cavernosal tissue regeneration, vascularization, and smooth muscle relaxation. However, the intracavernous injection of stem cells for erectile dysfunction treatment in clinical settings requires further investigation since its long-term efficacy and safety concerns regarding swelling, priapism, and fibrous plaque. Objective: This review aims to summarize the available evidence on the safety and efficacy of intracavernous stem cell injection for erectile dysfunction treatment in clinical studies only from PubMed. Conclusion: In conclusion, while the available evidence suggests that intracavernous stem cell injection is considered safe and effective for the treatment of erectile dysfunction, further large-scale clinical studies are needed to determine its long-term safety, efficacy, and optimal dosing regimens.
Effect of Time Transport on Mesenchymal Stem Cell Surface Markers: Unveiling the Influence of Cellular Translocation on Cellular Phenotype Wicaksono, Hendi; Prasetio, Ardi; Risky Chandra Satria Irawan; Naufal Ardjivani Arifin
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i4.29

Abstract

Mesenchymal stem cells (MSCs) hold great promise in regenerative medicine due to their ability to differentiate into multiple cell types and promote tissue repair. However, the effect of transportation time on the surface markers of MSCs remains understudied. This study investigated the impact of transportation time on MSC surface markers, specifically CD73, CD90, CD105, and hematopoietic lineage markers. MSCs were isolated from human umbilical cords and cultured. Flow cytometry analysis confirmed the expression of MSC surface markers. The MSCs were then subjected to simulated transportation for different time periods ranging from 0 to 24 hours at 2-8oC. After transportation, flow cytometry was used to analyze the expression of surface markers. The results showed that prolonged transportation time led to a decrease in the expression levels of CD73, CD90, and CD105, which are important markers for maintaining MSC functionality. Additionally, there was an increase in hematopoietic lineage marker expression. These findings suggest that transportation time can compromise the therapeutic potential of MSCs. Further investigation is needed to understand the underlying mechanisms responsible for the observed changes in surface marker expression. Optimization strategies, such as improved temperature control and protective agents, should be considered to mitigate the negative effects of prolonged transportation time. In conclusion, this study highlights the importance of considering transportation time and its impact on MSC surface markers in cellular therapy protocols. Understanding and addressing these effects are crucial for ensuring the quality and effectiveness of MSC-based therapies.
Secretome MSCs restore α-Smooth Muscle Actin Protein Tissue Expression in Croton Oil-Induced Hemorrhoid Rats Kusumo, M. Hidayat Budi; Husain, Sofian Azalia; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Hemorrhoidal disease, a prevalent and distressing condition affecting a significant number of the population, presents a considerable challenge in both clinical management and patient quality of life. Secretome mesenchymal stem cell hypoxia (S-HMSCs) is involved in accelerated remodeling and regeneration of wound tissue, including hemorrhoids, through anti-inflammatory and anti-fibrotic molecules paracrine activities. Objective: This study aims to investigate the effect of Secretome Hypoxia MSCs (S-HMSCs) in restoring α-smooth muscle actin (α-SMA) expression in croton oil-induced hemorrhoid rats. Material and Methods: An experimental study with a post-test-only control group design was used in this study. Croton oil was administrated for inducting hemorrhoidal disease. A total of 24 male Wistar rats were divided into four groups (n=6); Sham (Healthy group); Untreated (Croton oil+NaCl 300 µL) Croton oil+S-HMSCs 150); Secretome 150 µL (Croton oil+S-HM SCs 150 µL) and Secretome 300 µL (Croton oil+S-HMSCs 300 µL). S-HMSCs were injected intraperitoneally every week for up to 4 weeks. All animals were scarified and the rectal tissue was collected for α-SMA immunohistochemical staining analysis. Results: After hemorrhoid induction, α-SMA was expressed 20% higher than Sham group, furthermore, administration of 150 µL and 300 µL of S-HMSCs may decreased by 15% and 20% α-SMA expression compared to the Untreated group, expression in croton oil-induced hemorrhoid rats.
Comparison of Two Tangential Flow Filtration Methods in Isolating CD63+/CD9+ Mesenchymal Stem Cell Exosome Putra, Agung; Alif, Iffan; Prasetio, Ardi; Prawitasari, Salindri
International Journal of Cell and Biomedical Science Vol 2 No 4 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Extracellular vesicles, particularly CD63+/CD9+ Mesenchymal Stem Cell Exosome (MSC-Exo), have emerged as crucial mediators of intercellular communication and potential therapeutic agents, including regenerative medicine and immunomodulation. However, the precise isolation and purification of MSC exosomes pose critical challenges. Tangential Flow Filtration (TFF) has gained recognition as an efficient exosome isolation method, offering scalability and versatility. In this study, we address the pressing need for standardized exosome isolation methods by comparing two distinct TFF-based protocols for isolating CD63+/CD9+ MSC exosomes based on filter size pore order. Methods: MSC-Exo were conducted from the Stem Cell and Cancer Research Laboratory (SCCR Indonesia), which were then processed through TFF using different filter sizes and orders. There are two filtration methods compared, first, MSC-Exo was filtered with 1000-5-500-300-100-50-10-5 filter order. Second procedure, MSC-Exo was filtered using 1000-500-300-100-50-10-5 filter order. Result: Flow cytometry analysis revealed variations in the percentage of CD63+/CD9+ in the MSC-Exo based on filter order. The results indicate that the choice of filter order significantly influences the size range with the highest concentration of CD63+/CD9+ MSC-Exo. Conclusion: This research underscores the importance of optimizing TFF-based isolation methods for CD63+/CD9+ MSC exosomes, especially in the order of filter pore size.

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