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Contact Name
Nur Indah Septriani
Contact Email
nurindahseptriani@ugm.ac.id
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+628214423902
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bibjurnal.biologi@ugm.ac.id
Editorial Address
Fakultas Biologi, Universitas Gadjah Mada. Jl. Teknika Selatan, Sinduadi, Mlati, Sleman 55281
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Kab. sleman,
Daerah istimewa yogyakarta
INDONESIA
Berkala Ilmiah Biologi
ISSN : 08537240     EISSN : 29644429     DOI : https://doi.org/10.22146/bib.v14i3
Biologi Fungsional, Struktur dan Perkembangan, Bioteknologi dan Biologi Molekuler, serta Biologi Lingkungan dan Biodiversitas
Articles 59 Documents
Characteristics of Water Storage Capacity and Water Storage Efficiency of ‘Biotextile’ Growing Medium for Erosion Resistance Utami, Dyah Nursita; Kusumastuti, Etty; Sudiana, Nana; Rahayu, Budi; Hidayat, Nur; Sulistiawan, Insan Nur; Purnomo, Agus; Atang; Sittadewi, Euthalia Hanggari
Berkala Ilmiah Biologi Vol 14 No 1 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i1.5891

Abstract

Abstract: Biotextile is an innovation in erosion-resistant growing media technology, made from organic cocopeat supplemented with Legume Cover Crop (LCC) seeds and coated with biofertilizer. In its application, the success of biotextiles is determined, among other things, by the capacity and efficiency of water storage in the media The capacity and water storage efficiency of biotextile media will determine the success of growth in field applications. From field tests conducted on the sloping land of the Ciater reservoir cliffs, South Tangerang, it was shown that on the 4th day the LCC seeds had started to grow, on the 10th day they had grown in all biotextile cells, and at the age of 3 months the LCC plant canopy had covered almost all of the soil surface at the test field. Based on the growth test results, biotextile characterization tests have been carried out, which include measuring water content (KA), water storage capacity (KPA), and water storage efficiency (Es). Based on these test results, a biotextile characteristic test has been carried out which includes water content (KA), water storage capacity (KPA), and water storage efficiency (Es) to find out the characteristics of KPA and Es in evaluating its success and to prove the hypothesis that KPA and biotextile Es support the successful growth of land cover plants (LCC). The measurement of KA biotextile was carried out using the gravimetric method, while KPA and Es were measured using the immersion method, which was carried out in the Serpong laboratory. From the results of laboratory analysis, KA reached a range of values: 40–80%, KPA = 74.41–297.59%, and Es = 21%–75.5%. Apart from the results of plant growth and laboratory results, from a physical perspective, the performance of biotextiles can be seen in reducing the energy of rainwater blows, reducing the flow rate of surface water, and reducing soil erosion.
Anatomical Structure and Histochemical Study Male Strobilus of Melinjo (Gnetum gnemon L.) Wenni Eka Damayanti; Siti Susanti
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6321

Abstract

Melinjo (Gnetum gnemon L.) is a plant from the Gymnospermae has many benefit, one of them is the melinjo male strobilus which is used as a vegetable. In melinjo plants there are various kinds of secondary metabolite compounds, especially the organs of leaves, seeds and seed coats, so that apart from being used as food ingredient it can be used as traditional medicine. This study aims to determine the anatomical structure and content of secondary metabolites in melinjo male strobilus. Observations of anatomical structures were made using paraffin methods, and observations of secondary metabolite content used histochemical tests using reagents on slices of fresh preparations then observed with a light microscope and were documented using OptiLab. The results of the observations revealed that anatomical structure of the nodes and internodes at the base, middle, and tip does not revealed that there was structural differences that composed of epidermal tissue, parenchyma tissue, and vascular bundle with xylem and phloem, but there were differences in size that are getting smaller from the base to tip. The structure of the male flowers revealed that there were structural differences with differentiation from the tip to the base of the anther with increasingly size of the cells epidermal tissue, degradation of parenchyma tissue at the middle layer and tapetum, and pollen have been formed. Histochemical test at melinjo male strobilus contained secondary metabolites such as phenolics, terpenoids, flavonoids, alkaloids, and tannins.
The Potency of Olive Oil, Coconut Oil, and Bleached Palm Oil as Clearing Agent in Histological Preparation Ruth Liananda Citra Dolok Saribu; Laili Mufli Zusrina; Murtiadi Erlan Supraitno; Ardaning Nuriliani; Bambang Retnoaji; Hendry T.S.S.G. Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6528

Abstract

Clearing is an important process in histological preparations. The aim of the clearing process is to remove alcohol from the tissues upon dehydration. The most commonly used clearing agent is xylol, however it is known that there are negative effects from using xylol. This literature study purpose to study the potential of three vegetable oils, i.e., olive oil, coconut oil, and refined palm oil, as clearing agents to replace xylol. This literature study was conducted using search engines such as Semantic Scholar, NCBI, ICAMS, IJPRSE, JOMFP, PubMed, and ResearchGate with the keywords olive oil, coconut oil, palm oil, xylol, clearing agent, and histological preparations. There is no year limitation of references in this literature study. Based on the literatures, it is known that the use of olive oil as a clearing agent produces similar histological preparation qualities to xylol. This can be seen from several criteria that were measured, including the level of tissue translucency and the ability to maintain the quality of the staining. Similar results were shown by purified coconut oil and palm oil, both of which can produce tissues with optimal levels of transparency. The conclusion from this literature study is that refined olive oil, coconut oil, and palm oil can be used as clearing agents for xylol substitutes that are safer for researchers and the environment.
Fixative Solution for Macromolecules in Histological Preparations Hayu Swari Alimi; Ria Vena Septhay Sari; Tia Apriliyani; Ardaning Nuriliani; Bambang Retnoaji; Hendry Saragih; Zuliyati Rohmah
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.6531

Abstract

The fixation process is subjected to preserve cells, tissues, and their components so that the structure can be maintained as their alive condition and prevent autolysis. This literature review purpose to determine the appropriate use of fixative solutions for several macromolecules, such as proteins, lipids, carbohydrates, and nucleic acids. The literature review data is obtained form the secondary data included books, journals, or articles which discussed about fixative solutions without a limitation on the year of publication. The databases were sourced from Google Scholar, PubMed, NCBI, Science Direct, SpringerLink, Research Gate, and Nature. The keywords for the book search were tissue processing, pathology, and histology. Keywords for searching journals or articles were protein fixation, formaldehyde fixation lipids, fixatives for nucleic acids, Glyo-Fixx fixatives, mercury chloride fixative lipids, formalin-fixed carbohydrates, and ethanol or methanol for fixative lipids. Based on the literature review conducted, it can be concluded that the use of fixative solutions is according to the macromolecules to be observed.
Effect of Fermented Feed on Cattle Weight at Pondok Rawa Village, Deli Serdang Regency, North Sumatera Hesti Wahyuningsih; Hotnida Sinaga; Liana Dwi Sri Hastuti; Fachri Fauzi; Wulan Apridamayanti; Silfy Anisa Nasution; Wira Khairulsyah; Arbi Maulana
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6571

Abstract

Abstract: Cattle farming in Indonesia, especially in North Sumatra, is still a secondary livelihood for breeders. Treatment and processing of beef are generally done in the traditional way. The success of the beef cattle business is very dependent on the fulfillment of nutrients that affect livestock health. Therefore, quality and sustainable feed is an important factor in livestock cultivation technology. The purpose of this study was to determine the effect of fermented feed on cattle weight at a small cattle farm in Pondok Rawa Village, Deli Serdang Regency, Sumatera Utara. Fermented feed is formulated by mixing corn ash and palm oil cake (5:1), coarse salt, molasses, and EM4 into chlorine-free water. The feed was stored in PP plastic and incubated for 7 days. Five cows chosen randomly were given fermented feed every day for 1 month while the other 5 cows were only given forage. All test cattle were about 4 years old and weighed weekly and the data were statistically analyzed using ANOVA in JASP 0.16.4. The results showed that the average weight gain of one cattle fed with fermented feed (1.54 kg/week) was higher than forage treatment (0.69 kg/week). This result was validated and substantiated by ANOVA test result showing that value of p < 0.05.
Manokwari Wasti Lake Mangrove Forest Vegetation Structure, West Papua Semuel Sander Erari; Jan H. Nunaki; Sepus M Fatem
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.6890

Abstract

Wasti lake in Manokwari is one of the mangrove forest areas located on the coast of Manokwari and has ecological, economic and socio-cultural potential. Development continues, land conversion for settlement and economic development will have a negative impact on the sustainability of the mangrove ecosystem in Wasti Lake. Thus, this study aims to determine the vegetation structure of the mangrove forest at Telaga Wasti and its ecological potential to prepare teaching materials for ecology courses at the Biology Education Study Program, University of Papua. This research was conducted in May - July 2022. The method used is the line method (transect line) and the quadratic method. Data was collected on plots measuring 20m x 20m for tree vegetation, 10m x 10m for pole vegetation, 5m x 5m for saplings and 2m x 2m for seedlings. The results of data analysis showed that the mangrove vegetation structure consisted of 7 species from 4 families. The highest INP at tree level for both stations was Sonneratia alba, while the highest INP for pole level at station I was Rhizophora stylosa and station II was Sonneratia alba. The highest level of sapling at station I INP was Rhizophora stylosa and at station II the highest INP was R. apiculata. Then the highest INP station I seedling vegetation was R. stylosa and the highest INP Station II was R. apuculata.
Comparison of the Composition and Abundance of Phytoplankton Based on Different Land Use in the Cisadane River, Tangerang Regency Alifa Varmlandia; Suwarno Hadisusanto
Berkala Ilmiah Biologi Vol 14 No 2 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i2.7684

Abstract

Phytoplankton is an autotrophic organism that produce its own food through the process of photosynthesis with the help of sunlight which forms the basis of water productivity to be utilized for trophic organisms above. In freshwater, river plankton are categorized as potamoplankton. Rivers are a dominant freshwater ecosystem and have a more stable morphology than other freshwaters. Changes in land function is triggered by an increase in human population, an increase in the number of residential areas and industrial activities that discharge untreated waste into the river. Because of the importance of phytoplankton for life, it is necessary to conduct research on the Comparison of Phytoplankton Composition and Abundance Based on Different Land Uses in the Cisadane River, Tangerang Regency. In this study, sampling was carried out using a purposive sampling method by measuring parameters such as pH, temperature, and water transparency. Identification of phytoplankton composition and abundance was carried out using the Sedgwick Rafter Counting Cell (SRCC) using the sweep method. The results of this study obtained 23 phytoplankton genera from 6 classes consisting of: Bacillaryophyceae, Cyanophyceae, Chlorophyceae, Conjugatophyceae, Trebouxiophyceae, and Zygnematophyceae. Phytoplankton abundance was 185-490 Ind/L with the highest abundance in both areas are Bacillaryopiceae and Navicula. The diversity index was 1.307-2.124 and the dominance index was 0.201-0.461. Water quality parameters that affect the composition and abundance of phytoplankton are water-transparency, pH, and water temperature. The fertility level of the Cisadane River waters has an oligotrophic fertility level.
Identifikasi dan Seleksi Fungi Endofit Daun Sirih Hijau (Piper betle L.) Penghasil Enzim Selulase Dhia Salsabila Hakim; Rina Sri Kasiamdari
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.8714

Abstract

Abstract: Endophytic fungi were known in bioethanol industries due to their role in secreting secondary metabolites in the form of cellulolytic enzymes, which were known to be superior to bacteria. The secretion of metabolic enzymes depended on the host they invaded. Herbal plants were recognized as habitats for various endophytic fungi. Betel leaf (Piper betle L.) was one such herbal plant known for its high metabolite content. The aim of this study was to identify endophytic fungi living on betel leaf, conduct cellulolytic tests on the identified fungi, and determine which fungi exhibited the highest cellulolytic activity. The study yielded three species of endophytic fungi from betel leaf: Fusarium graminearum, Fusarium oxysporum, and Colletotrichum coccodes. All three samples showed positive results, producing cellulase enzymes characterized by the presence of a clear zone around the colonies. F. oxysporum was identified as the highest producer of cellulase enzymes, with a concentration of 0,033 U/ml.
Distribution and Habitat Characteristics of the Green Tree Frog (Rhacophorus reinwardtii Schlegel, 1840) in East Java. Mochammad Yordan Adi Pratama; Fatchur Rohman; Agus Dharmawan; Vivi Novianti; Sri Rahayu Lestar
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.9349

Abstract

The IUCN Red List classifies the green tree frog (Rhacophorus reinwardtii) as a frog that is Least Concern. One study that has never been carried out in Indonesia concerns the spatial distribution of the green tree frog based on Geographic Information System (GIS) applications and the habitat characteristics in East Java. It is vital to map the spatial distribution of the green tree frog in East Java and identify its habitat characteristics because the amphibian diversity surveys that have been conducted so far are the only ones available. This study is quantitative descriptive in design, with studies of pertinent literature as supporting sources. Cruising and the Visual Encounter Survey (VES) method are the techniques employed. A Geographic Information System (GIS) application is utilized in the computational method to create a spatial distribution analysis by gathering information on the locations of interactions with green tree frogs. Based on findings from studies conducted in the Bromo Tengger Semeru National Park Area (TNBTS), the Tancak Kembar Waterfall Area in Bondowoso Regency, and the Ironggolo Waterfall Area in Kediri Regency, there are 16 green tree frog meeting points recorded on the iNaturalist application for the Our Reptile Amphibian Observation Movement (GO-ARK) project and 3 points from the outcomes of field observations. In East Java, environmental factors such as altitude, slope, rainfall, NDVI, land cover, and proximity to rivers have a significant impact on how green tree frogs are distributed spatially. Although green tree frogs in East Java frequently forsake water bodies and choose to live in high-level plant types, they also frequently occupy a variety of water bodies. The green tree frogs discovered at the research location are categorized as favoring continuous habitats with boundaries for colony development.
Kemampuan Tumbuhan Lakum Air (Ludwigia octovalvis) sebagai Biofiltrator Kandungan Organik di Wilayah Perairan Rony Irawanto; Nadila Wulan Cahyani; Aunurohim; Tutik Nurhidayati; Edwin Setiawan
Berkala Ilmiah Biologi Vol 14 No 3 (2023)
Publisher : Fakultas Biologi Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/bib.v14i3.10136

Abstract

Indonesian waters are often faced with pollution problems caused by excess of organic content such as nitrate (NO3-) and phosphate (PO₄³⁻). For this reason, in managing these pollutants biofiltration can be applied. Biofiltration is a method for treating waste biologically and plants can be applied in reducing the content of pollutants Biofiltration can be done using. Therefore, we introduced “Lakum Air” plant (Ludwigia octovalvis) as biofiltrator for reducing organic content of nitrate (NO3-) and phosphate (PO₄³⁻). L. octovalvis were cultivated in media with addition of NO3- and PO₄³⁻ 10 mg/L respectively. The results displayed L. octovalvis in NO3- possessed an average reduction ith efficiency of 0% for 2 weeks contact time and 0.5% for 4 weeks. At the same time in PO₄³⁻, L. octovalvis possessed an average reduction with efficiency of 35% for 2 weeks contact time and 58% with 4 weeks. Moreover, L. octovalvis can absorb NO3- with an average of 2.99 mg/L in 2 weeks contact time and 5.92 mg/L in 4 weeks. Likewise, L. octovalvis absorb PO₄³⁻ with an average of 4.72 mg/L for 2 weeks contact time and 6.66 mg/L for 4 weeks.