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Journal of Fisheries & Marine
Published by Universitas Airlangga
ISSN : 20855842     EISSN : 25280759     DOI : https://doi.org/10.20473/jipk.v16i1.44436
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Ilmiah Perikanan dan Kelautan (JIPK; English: Scientific Journal of Fisheries and Marine) ISSN International Centre | ISSN:2528-0759 (Online) | ISSN: 2085-5842 (Print) JIPK is a peer-reviewed and open access biannually (April and November) that published by the Faculty of Fisheries and Marine, Universitas Airlangga. The aim of JIPK is to publish exciting, empirical research, recent science development, and high-quality science that addresses fundamental questions in fisheries and marine. JIPK only accepts manuscripts written in full English and processes submitted original script related of scope to fisheries and marine science and not being published by other publishers. We publish four categories of papers; 1) Original research papers, 2) Critical review articles, 3) Short communications, and, 4) Special Issue on applied or scientific research relevant to freshwater, brackish and marine environments. This journal gives readers the state of art of the theory and its applications of all aspects of fisheries and marine science. The scope of this journal includes, but is not limited to the research results of: Aquaculture, Fish Diseases, Marine science, Oceanography, Aquatic resources management, Fisheries product technology, Fish capture, technology, Fishery agribusiness, Fishery biotechnology/molecular genetics, Fish health management. JIPK has been indexed in Scopus, DOAJ, Sinta 1, Dimensions, Crossref, and others indexing. This journal has been accredited as a 1st Grade Scientific Journal (Sinta 1) by the Ministry of Research, Technology, and Higher Education of Indonesia since 8 December 2020.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia
Articles 560 Documents
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Effects of Vietnamese Balm (Elsholtzia ciliata (Thunb.) Hyland) Essential Oil on Growth Performance of Striped Catfish (Pangasianodon hypophthalmus) Juvenile stage: Effects of Vietnamese balm (Elsholtzia ciliata (Thunb.) Hyland) Tran Thi Phuong, Dung; Luong Thi Le, Tho; Nguyen Xuan, Tong; Luu Tang Phuc, Khang
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.56482

Abstract

Graphical Abstract Highlight Research Gas chromatography-mass spectrometry identified major components of the essential oil, including (Z)-β-Farnesene (24.02%), neral (15.06%), geranial (15.02%), and β-ocimene (13.61%). Diets enriched with 5% Vietnamese balm leaf essential oils significantly improved the survival rate, feed conversion ratio, and feed efficiency. Diets enriched with 5% Vietnamese balm leaf essential oils significantly improved the weight parameters (average weight; weight gain; percentage weight gain; percentage specific growth rate; daily weight gain). Diets enriched with 5% Vietnamese balm leaf essential oils significantly improved the length parameters (average length; length gain; percentage length gain; percentage specific growth rate; daily length gain). Abstract Vietnamese balm (Elsholtzia ciliata) is a prominent herb in Vietnamese traditional medicine. This study investigated the impact of Vietnamese balm leaf essential oils on the growth of striped catfish (Pangasianodon hypophthalmus) in the juvenile stage. Essential oils were extracted using steam distillation, following European Pharmacopoeia guidelines. Gas chromatography-mass spectrometry identified the essential oil components. The experiment encompassed three treatments: 0% (control), 3%, and 5% of Vietnamese balm leaf essential oils in a completely randomized design. The results demonstrated a time and dose-dependent modulation of striped catfish growth by the essential oils. Diets enriched with 5% Vietnamese balm leaf essential oils significantly enhanced survival rate and growth, indicating potential as a fish feed additive. The essential oil yield was 0.83% dry weight, with major components including (Z)-β-Farnesene (24.02%), neral (15.06%), geranial (15.02%), and β-ocimene (13.61%). Additionally, the study emphasized the growth-promoting effects of herbal extracts on various growth parameters. Further investigations should explore the impact of Vietnamese balm essential oil on catfish immune response and disease resistance, providing a comprehensive understanding of its potential benefits.
Concentration and Distribution of Oligochaeta Worms in the Waters of Kejapanan, Pasuruan, Indonesia Polluted by Mercury Waste using DNA Barcode Irawati Mei Widiastuti; Moh. Awaludin Adam; Ernawati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.56641

Abstract

Graphical Abstract Higlight Research Based on AAS test, results showed that the St2 sample has the highest concentration of mercury compared to other locations. St2 samples are samples taken right at the pollutant source. The results showed that the samples consist of the Nadidae family with two species, namely Limnodrilus hoffmeisteri and Branchiura sowerbyi. The COI gene that was successfully amplified had a length of approximately 700 bp using a 3000 bp DNA ladder as a comparison. Based on the results of the SEM-EDX test, the worm samples contained several elements. The majority of them are organic except Al, Si, and Ti. Aluminium (Al), Silicon (Si), and Titanium (Ti) are metals that are used by organisms.     Abstract Physiological monitoring of mercury waste contamination can be carried out using the biota around the waters. This study aims to identify concentration of Hg and the types of worms in the waters of Kejapanan, Pasuruan, East Java with a molecular approach. Target gene amplification was carried out using the mitochondrial genome COI barcode primer. Analysis of molecular identification was performed with DNA analysis and phylogenetic, similarity, DNA sequence variation, genetic distance, and the BOLD System. The concentration Hg was analyzed using AAS and the distribution of mercury in the worms was analyzed using SEM Edax Mapping. The results showed that the pollutant source area (St2 sample) has the highest concentration of mercury compared to other locations. The results of molecular identification indicate the formation of two clusters. The amplified samples produced DNA bands according to the target (600-700 bp), and the process was continued with morphological-based-key identification. The results showed that they consist of the family Nadidae with two species, namely Limnodrilus hoffmeisteri and Branchiura sowerbyi. A DNA length of 709 bp as well as nucleotide composition. BLAST results showed that species L. hoffmeisteri and B. sowerbyi had similarity indexes of 99% and 86%, respectively. Based on the research results, it was found that there was an accumulation of mercury exposure in worms in polluted areas. For this reason, the results of this study can provide a novelty that worms can be used as biomonitoring of water pollution using the barcode data.
The Genetic Diversity of Bali Sardinella (Sardinella lemuru) Caught at Bali Strait and Its Possible Relation to The Ocean Circulation Sartimbul, Aida; Hendiari, I Gusti Ayu Diah; Arthana, I Wayan; Kartika, Gde Raka Angga; Winata, Victor Adi; Pranowo, Widodo Setiyo; Susanto, Raden Dwi; Rohadi, Erfan; Muslihah, Nurul; Yona, Defri; Iranawati, Feni
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i2.56670

Abstract

Graphical Abstract Highlight Research A low sequence divergence of lemuru between Bali Strait and the northern latitude. S. lemuru migration route through ITF will maintain its nucleotide variation. The high haplotype diversity of lemuru is a testament to its adaptability. S. lemuru's high haplotype diversity supports their stock recovery in the Bali Strait. Two sardine species are morphologically and genetically highly distinct, even though they prefer overlapping water mass conditions from each other. Abstract Sardinella lemuru is known as the native pelagic fish of the Bali Strait. However, its presence has recently become challenging to find. This study aimed to validate S. lemuru using molecular taxonomy and link it to water circulations for sustainable management. It is the first study of relatively complete sequence data on S. lemuru in the Bali Strait. A total of 47 sequences were amplified using the control region gene of mitochondrial DNA. Other S. lemuru (n = 4) and Amblygaster sirm (n = 12) sequences were retrieved from GenBank. Low sequence divergence is observed in clade-1 of S. lemuru across the Bali Strait, Manado-Sulawesi, and Kudat-Malaysia. This is probably due to its migration using the main Indonesian ThroughFlow (ITF). There is an evolutionary divergence between two groups of S. lemurs, measuring 35.3%±4.1. The first signs of these cryptic groups were found at Canggu and Prigi, with 87 base-pair differences between the two groups. This study also presents strong evidence that A. sirm prefers overlapping water mass conditions with S. lemuru in the Bali Strait but is morphologically and genetically distinct. Notably, three samples of A. sirm in Kedonganan-Bali are clustered with other A. sirm locations passed by the primary and secondary South China Sea throughflow. The high genetic diversity of S. lemuru among Bali Strait sites demonstrates its adaptive ability, with scattered single-frequency haplotypes. The adaptive ability traits of S. lemuru support its stock recovery. The stock recovery recommendation is based on finding S. lemuru’s adaptive traits within their populations in suitable habitats and climatic conditions
Susceptibility and Target Organ of Lymphocystis Disease Virus Infection in Giant Gourami (Osphronemus goramy), Hybrid Tilapia (Oreochromis sp.), Siamese Fighting Fish (Betta splendens), and Hybrid Catfish (Clarias sp.) Nikmah, Nur Lailatul Fitrotun; Isnansetyo, Alim; Istiqomah, Indah; Murwantoko, Murwantoko
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.56685

Abstract

  Highlight Research 1. Lymphocystis disease is reported to infect seawater and freshwater fishes 2. The four important freshwater fish species in Indonesia are evaluated on their susceptibility to Lymphocystis Disease Virus 3. Lymphocystis infection causes behavioural changes and mortality with different onset times after infection on the four fish species 4. LCDV load is varied in quantity among different organs. Abstract Lymphocystis disease has a broad host range and has been reported to enter Indonesia. However, information regarding its susceptibility and predilection organs in fish is lacking. This study examined the susceptibility of four important fish species in Indonesia, namely, giant gourami (Osphronemus goramy), hybrid tilapia (Oreochromis sp.), Siamese fighting fish (Betta splendens), and hybrid catfish (Clarias sp.). The fish were infected with virus filtrate by intraperitoneal injection and immersion. The postinfection observation period was 60 days. Viral load was quantified by qPCR and expressed as major capsid protein (MCP) copy number/mg tissue. Mortality was observed in all fish species, with the highest recorded in hybrid catfish and the lowest in Siamese fighting fish. All the fish species showed changes in their clinical symptoms, such as anorexia and separation from schools. However, only giant gourami showed internal change seven days after injection (dpi), with white lesion detected in the liver. Viral load quantification showed that LCDV had different predilection organs in the four fish species. The highest viral load of giant gourami (1.7 x 104) was observed in the liver at 7 dpi, hybrid tilapia (7.5 x 103) was observed in the fins at 21 dpi, Siamese fighting fish (8.4 x 103) was observed in the fins at 14 dpi, and hybrid catfish (1.2 x 103) were observed in the fins and gills at 7 and 14 dpi. The findings indicated that giant gourami, hybrid tilapia, Siamese fighting fish, and hybrid catfish were susceptible to LCDV infection with different predilection organs.
Effects of the Combination of Honey and Euphorbia hirta on Controlling Aeromonas hydrophila in Catfish (Clarias gariepinus) Salosso, Yuliana; Rahma, Andi Taufika; Djonu, Asriati; Fransira, Immaria
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i2.56814

Abstract

Graphical Abstract Research Highlights The combination of honey and hirta improved hematological parameters of C. gariepinus catfish infected with A. hydrophila bacteria. The combination of honey and hirta positive affected the histopathology of liver, kidneys, and gills, which led to a restoration of organ function to normal levels. The combination of honey and hirta also improved the morphology of C. gariepinus. The most effective combination of honey and hirta was treatment C (1:2). Abstract Honey and Euphorbia hirta are natural ingredients that may serve as alternative treatments for Clarias gariepinus catfish infected with Aeromonas hydrophila bacteria. Both honey and E. hirta contain bioactive compounds with known antibacterial properties. This study aims to investigate the effects of combining honey and E. hirta on various hematological parameters, including erythrocytes, leukocytes, MCH, MCV, as well as histopathological and morphological changes in C. gariepinus catfish infected with A. hydrophila. This study used an experimental design involving three treatment combinations of honey and E. hirta, which were A (2:1), B (1:1), C (1:2) and a control with three replications. The treatments were administered to the fish through immersion. Honey was diluted at a 50% concentration in distilled water. Meanwhile, E. hirta was prepared as a coarse powder and boiled at a 3% concentration. The results showed that the combination of honey and E. hirta affected the hematological, histopathological, and morphological parameters of C. gariepinus catfish within the normal ranges. The most effective treatment was found to be treatment C (1:2). Therefore, the combination of honey and E. hirta is potential as a therapeutic option for bacterial infections in fish.
Nutritional Composition, Physicochemical, and Sensory Properties of Snack Bars Produced from Catfish Head Powder (Clarias gariepinus) and Purple Sweet Potatoes (Ipomoea batatas var Ayumurasaki) as Emergency Food Canti, Meda; Kasanti, Nafera Arvianda; Hutagalung, Rory Anthony; Lukas; Pandjaitan, M. M. Lanny W.
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i2.56831

Abstract

Graphical Abstract   Highlight Research 1. The development of snack bars from catfish head powder (CHP). 2. The CHP was rich in nutritional composition and could be an additive ingredient in snack bars. 3. Adding CHP increased protein, lipid, and fibre content in the snack bars. 4. Snack bars with the addition of CHP were acceptable on all sensory attributes. 5. Snack bars produced from CHP could be used as an alternative emergency food.     Abstract During natural disasters, emergency food is often needed to meet the victims’ nutritional intake such as protein, fat, carbohydrates, and energy. As an alternative, catfish heads, which are usually rich in nutritional content, can be developed into a snack bar product as emergency food. This research aimed to evaluate the chemical, physical and sensory properties of snack bars produced from catfish head powder (CHP) and purple sweet potato flour (PSPF). This research consisted of several stages, namely making CHP and PSPF, preparing snack bars, and analysing snack bars’ chemical, physical, and sensory properties. The ratios of CHP and PSPF in the preparation of snack bars stage were F0 (0:100), F1 (25:75), F2 (50:50), and F3 (75:25). The CHP contained 43.52% protein, 21.24% lipid, 4.6% fiber, and 384.38 Kcal/100 g total energy. The addition of CHP and PSPF affected the chemical (proximate and antioxidant activity), physical (redness and hardness values), and sensory (aroma and overall acceptance) properties of the produced snack bars. All snack bars had protein content that complied with Indonesian National Standards (INS) for cereal bar biscuits. The protein, lipid, carbohydrate, and total energy content of the snack bar were in accord with the standards of the United States Department of Agriculture (USDA), Food Standards Australia New Zealand (FSANZ), and emergency food standards. It is also revealed that F2 was the best treatment based on its chemical, physical, and sensory properties. Indeed, snack bars from CHP and PSPF can be utilized as an emergency food alternative.
Growth and Survival Rate of Pocillopora spp. Fragments on Coral Tree and Coral Table Media in Semut Kecil Island, Anambas Islands Ariqa Iriani, Fringgar; Arifin , Reyhan; Jerry Winata, Marcellinus; Rifat Muharam , Muhammad; Permata Putri , Novita; Dewi Ruswanti, Corina; Rahman Ritonga, Abdul; Riviani , Riviani; Riyanti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 1 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.vi.56844

Abstract

Graphical Abstract   Highlight Research Pocillopora spp. grew faster on coral tree media (0.55 cm/month) than on coral table media (0.15 cm/month). Survival rates were high, with 97.3% on coral trees and 87.0% on coral tables, proving both methods effective. Coral growth on table media was slower due to sediment accumulation, while tree media provided more stable growth. ANOVA analysis showed no significant effect of transplant media on growth and survival, confirming adaptability. Both methods are viable for large-scale coral reef restoration, especially in the Anambas Islands and similar ecosystems.     Abstract Pulau Semut Kecil, Anambas Islands, are located within the coral reef triangle, as one of the largest Marine Protected Areas (MPAs) in Indonesia. Coral reefs possess significant functional attributes within ecosystems, and their condition is progressively deteriorating due to anthropogenic activities. This situation necessitates restoration endeavors, primarily through coral transplantation methodologies. The methods involved the introduction or relocation of donor corals into substrates such as tree transplantation media and tables, exhibiting high success rates. This study was conducted to determine the growth and survival rate of Pocillopora spp. The direct survey approach was used and the different coral transplanting media were statistically analyzed with the ANOVA test and SPSS software. The results of the growth of coral fragments Pocillopora spp. Reported coral tree media and table with an average value of 0.55 cm/month and 0.15 cm/month. The survival rate of Pocillopora spp. coral fragments in tree media and table was 97.3% and 87.0%. The media for dropping coral trees and table did not affect the growth and survival rate. This coral transplantation method can facilitate for the restoration of degraded ecosystem and ecological succession.
Genetic Diversity and Population Structure of Amphidromous Goby (Stiphodon semoni) in Western Part of Southern Java Waters Romdon, Ahmad; Simanjuntak, Charles P. H.; Sulistiono; Syahailatua, Augy
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.57014

Abstract

Graphical Abstract Research Highlight The genetic diversity of Stiphodon semoni is high. All populations have high haplotype diversity except for Cimaja. No genetic structure is observed within the populations of semoni. The population of semoni has undergone demographic expansion. Cibareno River has the potential to be a protected area. Abstract Fishing activities negatively impact fish populations, potentially causing a decline in fish stocks. Nevertheless, ensuring diversity and connectivity among populations can mitigate these adverse effects. To evaluate the connectivity of river mouths in the western part of Southern Java waters, we sequenced forty Stiphodon semoni individuals from five populations using mitochondrial cytochrome oxidase subunit 1  as molecular markers. The study revealed that S. semoni populations showed high diversity (0.821), with the population in Cimaja displaying the lowest diversity (0.464). Furthermore, the result of the analysis of molecular variance was a Fst value of 0.0630 with a p-value of 0.22. These results along with the result of the haplotype network indicated no significant genetic differences among these populations. This implies that the river mouths in the western part of Southern Java waters are interconnected. The distribution of mismatches showed a single peak, indicating that the populations have undergone demographic expansion. This information could be valuable for the conservation and management of S. semoni in the western part of the Southern Java waters.
Use of Fruit Waste as Natural Dyes in pH-Sensitive Colorimetric Sensors for Tilapia Fillets Quality Decline Audrynachristie, Brigitta Stella; Alamsjah, Mochammad Amin; Kismiyati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v17i2.57245

Abstract

Graphical Abstract Highlight Research 1. The use of dragon fruit peel, mangosteen peel and onion peel waste as natural dyes in colorimetric sensors was analyzed in this study. 2. Natural dyes are added to bioplastics with kappa carrageenan polymer, a mixture of corn starch and kappa carrageenan, and corn starch to determine the characteristics of the best bioplastic to be used as a colorimetry sensor. 3. The quality of the fish fillet compared to the color changes in colorimetry sensor was analysed in this study. 4. Mixture of kappa carrageenan and corn starch as bioplastic with casting method was analyzed in this study. Abstract Tilapia fillet aimed to prolonging shelf life, may still experience quality deterioration posing food safety risks. Colorimetric pH indicators offer a simple and affordable solution to the food industry to evaluating fish spoilage. Natural dyes reduce potential health risks associated with synthetic dyes. Anthocyanin sources which haven’t been explored like dragon fruit rind, mangosteen rind, and red onion skin serve as real time quality and safety assessment tools for consumers. This study explores the potential of anthocyanin extracted from dragon fruit rind, mangosteen rind, and red onion skin as colorimetric sensors for evaluating the quality and safety of tilapia fillets. The anthocyanin-based sensors developed using kappa carrageenan and corn starch polymers to achieve sustainability fisheries program and were characterized according to the Japanese Industrial Standard for bioplastics. The results show that anthocyanin from dragon fruit skin exhibited the best color change in response to pH changes in the tilapia fillet, indicating its potential as a reliable indicator of spoilage. This research highlights the feasibility of using natural dyes as colorimetric sensors, reducing the risk of health hazards associated with synthetic dyes. This study also shows that different polymers give different characteristic of bioplastic. Carrageenan bioplastic shows best thickness values of 0,118 mm; Carrageenan and corn starch bioplastics show best tensile strength values of 20,62 MPa; Carrageenan bioplastic shows best elongation values of 254%; and all polymers show the same biodegradation rate values of 14,29%. Further studies are needed to explore other natural dyes and optimize the polymers for optimal bioplastic characteristics.
Stability and Efficacy of Live-Attenuated Vibrio harveyi Vaccines Under Different Storage Conditions in Zebrafish (Danio rerio) Models Muhamad-Sofie, Mohd Hafiz Ngoo; Mohamad, Aslah; Azzam-Sayuti, Mohamad; Amal, Mohammad Noor Azmai; Zamri-Saad, Mohd; Monir, Md Shirajum; Md Yasin, Ina Salwany
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.59794

Abstract

Graphical Abstract Highlight Research The LD50 (median lethal dose) of Vibrio parahaemolyticus and harveyi in zebrafish was determined to be 1 x 106 CFU/mL, while for V. alginolyticus it was found to be 1 x 105 CFU/mL. The LAVh vaccine demonstrated cross-protection against various pathogenic strains of Vibrio, leading to an average of 80% survival rate in vaccinated individuals. The analysis of the LAVh vaccine emphasized its versatility, as it can be quickly deployed and stored as a freeze-dried powder. The LAVh vaccine can be easily accessible and user-friendly in various aquaculture environments, such as offshore and remote farms. Abstract Vibriosis poses a significant threat to marine teleosts, causing substantial losses in the global aquaculture industry. Previous work in our lab led to the development of a live-attenuated V. harveyi vaccine (LAVh) candidate that targets the serine endoprotease gene with a three-point knockout and has shown promise in protecting against vibriosis. However, further investigation is necessary to evaluate the stability and efficacy of its various storage conditions for broader applications. This study aims to determine how well the three different LAVh vaccine storage (fresh, stale, and freeze-dried LAVh) worked against vibriosis. A total of 1000 adult zebrafish (Danio rerio) (mean weight: 0.20±0.5 g) were divided into four groups. Groups 1, 2, and 3 were intraperitoneally injected with different LAVh vaccine storage (fresh, stale, and freeze-dried, respectively), while Group 4 received 0.01 M phosphate-buffered saline (PBS) and served as the unvaccinated control. Fish were monitored for 21 days post-vaccination for safety, stability, efficacy, and antibody analysis. The results showed that a modest dosage of 1 x 104 CFU/mL of LAVh vaccine from all storage conditions provided 80% survival upon intraperitoneal challenge with pathogenic strains of pathogenic V. harveyi, V. alginolyticus, and V. parahaemolyticus. This dosage induced significant antibody production and conferred cross-protection against different Vibrio spp., indicating the LAVh vaccine’s potential for commercial application. The LAVh vaccine demonstrated high effectiveness and suitability for storage as a freeze-dried powder. This study might offer significant insights into practical strategies for reducing vibriosis, especially in aquaculture settings with limited infrastructure.

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