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Animal Husbandry Department, The Faculty of Agriculture, Universitas Syiah Kuala Jln. Tgk. H. Hasan Krueng Kalee No. 3, Kopelma Darussalam, Banda Aceh, 23111, Aceh, Indonesia
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INDONESIA
Jurnal Agripet
Published by Universitas Syiah Kuala
ISSN : 14114623     EISSN : 24604534     DOI : https://doi.org/10.17969/agripet
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Aims Jurnal Agripet aims to publish original research results on farm tropical animals such as cattle, buffaloes, sheep, goats, horses, poultry, etc. Scope Jurnal Agripet encompasses a broad range of research topics in animal sciences and biotechnology including animal physiology and nutrition, feed processing and technology, animal productions, animal genetics, breeding and reproduction, meat and milk sciences, animal health, behavior and welfare, animal housing and livestock farming system.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi
Articles 462 Documents
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Expression of Lon-like Protease Gene from Lactobacillus plantarum IIA-1A5 in Escherichia coli BL21(DE3) Olfa Mega; Cece Sumantri; Irma Isnafia Arief; Cahyo Budiman
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14904

Abstract

Proteases are one of most important and abundant enzymes produced by the biotechnology industry, for scientific, physiological and industrial application and dominates of the whole enzyme market. Lactobacillus plantarum IIA-1A5 is an Indonesian lactic acid bacteria (LAB) isolated from beef Peranakan Ongole cattle. Preliminary analysis on its whole genome sequence indicated that this strain harbours some genes involved in protein degradation and might be promising to be further applied. This study aims to optimize the gene sequence of a lon-like protease of L. plantarum IIA-1A5 for heterologous expression system. The Lon-like gene expression system is made using genes that have been optimized first in silico. pET-28a(+), E. coli BL21(DE3), Nde1 and BamH1 were used in this study as a expression vector, a host and retriction enzyme, respectively. Molecular weight was validated using SDS-PAGE and expasy.org software. The results showed that optimization increased codon adaptation index value (CAI) and GC content to 0.97 and 56.57%, respectively, which were suitable for the E. coli expression system. The Lon-like IIA gene was successfully expressed in the cell cytoplasm by induction of 1 mM Isopropyl -D-1-thiogalactopyranoside (IPTG) at 37 C. As many as 88% of Lon-like IIA codons were distributed in the 91-100 quality group. Lon-like IIA was successfully expressed in a host cell induced with 1 mM IPTG at 37oC . IPTG induction was performed at the 3rd hour of incubation with OD600 0.59. In addition, Lon-like IIA molecular weight was detected approximately 43 kDa.
Pengaruh Perbedaan Suhu Pemanasan terhadap Aktivitas Antibakteri Susu Kambing Fermentasi Menggunakan Lactobacillus Plantarum Zuraida Hanum; Yurliasni Yurliasni; Sitti Wajizah; Juanda Juanda
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14330

Abstract

ABSTRAK. Penelitian ini bertujuan untuk melihat pengaruh suhu pemanasan yang berbeda terhadap aktivitas antibakteri susu kambing fermentasi menggunakan starter Lactobacillus plantarum. Bakteri uji yang digunakan berasal dari golongan Enterobacteriaceae yaitu Escherichia coli dan Salmonella.sp. Rancangan penelitian yang digunakan adalah RAL (Rancangan Acak Lengkap) Faktorial yang terdiri dari 2 faktor yaitu faktor A adalah suhu pemanasan, yaitu a1 (suhu pemanasan 72C), a2 (suhu pemanasan 121C) dan faktor B adalah level penambahan Lactobacillus plantarum yaitu perlakuan b1 (Lactobacillus plantarum 2,5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7,5%), masing-masing perlakuan diulang sebanyak 3 kali sehingga diperoleh 18 unit percobaan. Apabila terdapat perbedaan yang nyata maka dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan adanya interaksi dari suhu pemanasan yang berbeda dan penambahan L. plantarum pada susu kambing berpengaruh nyata terhadap kadar asam laktat dan daya hambat pada Salmonella sp, akan tetapi tidak berpengaruh nyata terhadap E. coli. Kesimpulan dari penelitian ini diperoleh suhu pasteurisasi dengan penambahan 7.5 % L.plantarum merupakan nilai terbaik dalam menghambat Salmonella dan E..coli.(Effect of different heating temperature on antibacterial activity of goat milk fermented with Lactobacillus Plantarum)ABSTRACT.This study aimed to determine the effect of the different heating temperatures on inhibitory activity of fermented goat's milk with Lactobacillus plantarum. The test bacteria used were Enterobacteriaceae, namely Escherichia coli and Salmonella.sp.The design applied is CFD (Completely Randomized Design) consisting of 2 factors, namely factor A heating temperature with two levels a1 (72C), a2 (121C) and factor B is the level of Lactobacillus plantarum addition by three levels, namely b1 (Lactobacillus plantarum 2.5%), b2 (Lactobacillus plantarum 5%), b3 (Lactobacillus plantarum 7.5%), and the treatments were repeated 3 times. The results showed that different heating temperature and level of L. plantarum added in fermented goat milk significantly affected the levels of lactic acid and inhibitory power on Salmonella sp, but not on E. coli. In conclusion is different heating temperatures and addition of L. plantarum on goat milk at significantly affected on inhibitory power on Salmonella sp, but not on E. coli. The results obtained pasteurisasi with 7.5% L.plantarum could be applied.
Evaluasi Kinerja Pertumbuhan Sapi Silangan Belgian Blue dan Peranakan Ongole Jakaria Jakaria; Edwar Edwar; Mokhamad Fakhrul Ulum; Rudy Priyanto
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.15022

Abstract

ABSTRAK. Tujuan penelitian ini adalah mengevaluasi kinerja pertumbuhan sapi persilangan Belgian Blue (BB) dan sapi Peranakan Ongole (PO) pada turunan pertama (F1). Sapi persilangan (BB dan PO) yang digunakan sebanyak 15 ekor terdiri atas jantan 6 ekor dan betina 9 ekor, selain itu digunakan 8 ekor sapi PO betina yang dipelihara di Balai Embrio Ternak (BET) Cipelang Bogor. Peubah yang diamati adalah bobot lahir (BW), bobot sapih (WW) 205 hari, bobot 1 tahun (YW), pertambahan bobot badan harian dari lahir (GBW) sampai sapih (GWW) dan pertambahan bobot badan harian dari sapih (GWW) sampai umur 1 tahun (GYW). Data dianalisis secara deskriptif dan perbedaan antar sapi silangan (BB-PO) dengan sapi PO dilakukan uji t. Hasil analisis bobot badan dan pertumbuhan menunjukkan bahwa, bobot badan sapi silangan BB dan PO lebih tinggi dibandingkan dengan sapi PO pada WW 205 hari (165,221,3 kg dan 11515,2 kg), YW (365 hari) (240,728,1 kg dan 194,926,1 kg) dan GBW sampai GWW (0,6750,097 kg dan 0,4410,059 kg). BW dan GWW sampai GYW tidak berbeda antara sapi silangan BB-PO dengan PO. Hasil penelitian ini menunjukkan bahwa program cross-breeding sapi BB dengan PO dapat meningkatkan kinerja bobot badan dan pertumbuhan.(Growth performance evaluation of belgian blue and ongole crossbreed)ABSTRACT. The purpose of this study was to evaluate the growth performance of cross-breeding of Belgian Blue (BB) and Ongole Grade (PO) cattle in the first generation (F1). Crossbred of BB and PO cattle used as many as 15 individuals consisting of 6 males and 9 females, also, 8 individual PO females are used which are kept in the Livestock Embryo Center (BET) Cipelang, Bogor. The observed variables were birth weight (BW), weaning weight (WW) 205 days, 1-year weight (YW) 365 days, daily body weight gain from birth (GBW) to weaning (GWW) and daily body weight gain from weaning (GWW) until the age of 1 year (GYW). Data were analyzed descriptively and the difference between cross-breeding (BB-PO) cattle and PO cattle was analyzed by t-test. The results of body weight and growth analysis showed that crossbred BB and PO cattle were higher than PO cattle at WW 205 days (165.2 21.3 kg and 115 15.2 kg), YW (365 days) (240.7 28.1 kg and 194.9 26.1 kg ) and GBW to GWW (0.675 0.097 kg and 0.441 0.059 kg). BW and GWW to GYW do not differ between crossbred BB-PO and PO cattle. The results of this study indicate that the cross-breeding program for BB cattle with PO cattle breeds can improve the performance of body weight and growth.
Penerapan Pendekatan dan Metode Penyuluhan dalam Diseminasi Inovasi Inseminasi Buatan (IB) pada Usaha Peternakan Sapi Potong di Kecamatan Pauh, Kota Padang Ediset Ediset; Amrizal Anas
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14956

Abstract

ABSTRAK. Penelitian dilaksanakan di salah satu daerah sentra peternakan sapi potong, yaitu Kecamatan Pauh, Kota Padang, Sumatera Barat, yang bertujuan untuk mengetahui pendekatan penyuluhan dalam diseminasi inovasi Inseminasi Buatan (IB) pada peternak sapi potong dan metode penyuluhan dalam diseminasi inovasi Inseminasi Buatan (IB) pada peternak sapi potong. Penelitian ini menggunakan pendekatan metode survei melalui observasi, wawancara, dan dokumen data. Populasi adalah peternak sapi potong yang sudah mengadopsi inovasi Inseminasi Buatan (IB), Sampel ditetapkan sebanyak 30 peternak dengan teknik Quota Sampling. Analisis data dilakukan secara deskriptif kuantitatif yang dihitung dengan menggunakan skala Likert. Hasil penelitian adalah penyuluh sudah menerapkan pendekatan persuasif, pendekatan pervasion dan pendekatan compulsion dengan baik, sedangkan pendekatan coersion tidak diterapkan dalam diseminasi inovasi IB. Metode kunjungan rumah / usaha tani dan metode demonstrasi sudah diterapkan dengan baik, sedangkan metode kampanye tidak diterapkan oleh penyuluh dalam diseminasi inovasi IB pada peternak sapi potong di Kota Padang.(The application of extension approaches and methods in disseminating artificial insemination (AI) innovation to beef cattle farmers at Pauh sub-district in Padang)ABSTRACT. A survey was carried out at Pauh sub-district, Padang to understand extension approach and method in disseminating artificial insemination (AI) innovation to beef cattle farmers. A survey method approach was used through observation, interviews and data document. Primary data were collected from 30 farmers using quota sampling technique. Data analysis was performed descriptively quantitative which was calculated using a Likert scale. The results showed that the extension workers have applied a convincing approach in both persuasive and compulsion types. However they did not use coercion in conveying AI innovation. Farm visit as well as demonstration have been implemented well by extension workers, while the campaign method is not applied in disseminating IB innovations to beef cattle breeders in the city of Padang.
Evaluasi Kualitas, Karakteristik Fermentasi dan Kecernaan In Vitro Silase Campuran Sorgum Stay Green-Gliricidia sepium dengan Penambahan Berbagai Level Asam Laktat Widhi Kurniawan; Syamsuddin Syamsuddin; Wa Laili Salid; Purnaning Dhian Isnaini
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14857

Abstract

ABSTRAK. Silase gabungan rumput-leguminosa merupakan inovasi yang dapat diaplikasikan untuk menyediakan pakan dan bisa digunakan sepanjang tahun. Kandungan protein silase campuran tersebut dapat mencapai tingkat optimal sebagai pakan konsentrat hijau (green concentrate). Penelitian ini bertujuan untuk mengevaluasi kualitas, karakter fermentasi dan kecernaan in vitro silase campuran sorgum (Sorghum bicolor(L.) Moench) and gamal (Gliricidia sepium) dengan menambahkan dosis asam laktat yang berbeda. Silase dibuat dengan mencampur bahan penyusun yang terdiri dari sorgum dan daun gamal dengan perbandingan 1:1 dan penambahan asam laktat konsentrasi 20% (v/v). Penelitian terdiri atas 3 perlakuan yaitu; A0: (kontrol) tanpa penambahan asam laktat, A1: penambahan asam laktat sebanyak 2% (b/v), A2: 2,5% (b/v), dan A3: 3% (b/v) dengan 4 ulangan tiap perlakuan. Proses ensilase menggunakan cairan rumen kambing selama 21 hari. Parameter penelitian yang diamati adalah kualitas silase dan kecernaan in vitro. Hasil penelitian menunjukkan bahwa dosis penambahan asam laktat sebanyak 2% menghasilkan silase paling optimal dengan pH (3,94), bahan kering (26,62%), dan nilai Fleigh (100,89), namun tidak berpengaruh terhadap kandungan protein kasar, kecernaan bahan organik, kecernaan bahan kering dan kecernaan protein kasar. Kesimpulan pada penelitian ini adalah penggunaan asam laktat organik dengan dosis 2 hingga 3% (b/v) konsentrasi 20% (v/v) mampu meningkatkan karakteristik, kualitas fermentasi tetapi tidak mempengaruhi nilai KcBK, KcBO dan KcPK silase campuran sorgum stay green (Sorghum bicolor(L.) Moench) and gamal (Gliricidia sepium).(Quality evaluation, fermentation characteristic and in vitro digestibility of stay green sorghum-Gliricidia sepium mix silage on different level of lactic acid utilization)ABSTRACT. The grass-legume combined silage is an innovation that can be applied to provide feed and can be used throughout the year. The content of the mixed silage protein can reach optimal levels as a green concentrate feed. This study aims to evaluate the quality, fermentation characteristics and digestibility of in vitro silage of a mixture of sorghum (Sorghum bicolor(L.) Moench) and gamal (Gliricidia sepium) by adding different doses of lactic acid. Silage is made by mixing a constituent consisting of sorghum and gamal leaves in a ratio of 1: 1 and the addition of lactic acid concentration of 20% (v / v). The study consisted of 3 treatments namely; A0: (control) without the addition of lactic acid, A1: addition of 2% (w / v) lactic acid, A2: 2.5% (w / v), and A3: 3% (w / v) with 4 replications each treatment. The ensilage process used goat's rumen fluid for 21 days. The research parameters observed were silage quality and in vitro digestibility. The results showed that the addition dose of lactic acid as much as 2% produced the most optimal silage with pH (3.94), dry matter (26.62%), and Fleigh value (100.89), but had no effect on crude protein content, digestibility organic matter, digestibility of dry matter and digestibility of crude protein. The conclusion of this study is the use of organic lactic acid with a dose of 2 to 3% (w/v) concentration of 20% (v/v) can improve the characteristics, quality of fermentation but does not affect the value of KcBK, KcBO and KcPK silage stay sorghum mixture green (Sorghum bicolor (L.) Moench) and gamal (Gliricidia sepium).
Respon Fisiologis dan Penyusutan Bobot Badan Domba Lokal Jantan terhadap Transportasi dengan Posisi Berbeda dalam Kendaraan Lendrawati Lendrawati; Rudi Priyanto; Mohamad Yamin; Anuraga Jayanegara; Wasmen Manalu; Desrial Desrial
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14877

Abstract

ABSTRAK. Penelitian ini bertujuan untuk mengevaluasi respon fisiologis dan penyusutan bobot badan domba lokal jantan terhadap transportasi dengan posisi yang berbeda pada kendaraan. Penelitian ini menggunakan 10 ekor domba lokal jantan yang berumur 8-10 bulan dengan rataan bobot badan 16,11 2,27 kg. Penelitian ini menggunkan Rancangan Acak Lengkap dua faktor dengan 5 ulangan. Faktor pertama adalah: posisi domba di kendaraan terdiri dari: posisi berdiri dan berbaring. Faktor kedua adalah waktu pengambilan sampel yaitu: sebelum dan setelah transportasi. Semua domba perlakuan diangkut dalam satu mobil Pick up selama 8 jam perjalanan dengan tingkat kepadatan yang sama yaitu 0,27 m2/ekor. Data bobot badan, suhu rektal, laju respirasi, denyut nadi, hormon kortisol, glukosa, kreatinin, hemoglobin dan hematokrit dikumpulkan sebelum dan setelah transportasi. Hasil penelitian menunjukkan bahwa tidak terdapat interaksi yang nyata (P0,05) antara posisi domba dikendaraan dengan waktu pengambilan sampel pada semua parameter (kecuali denyut nadi). Penempatan domba dengan posisi berdiri dan berbaring selama 8 jam transportasi memperlihatkan respon yang sama terhadap penyusutan bobot badan, suhu rektal, laju pernapasan, hormon kortisol, konsentrasi glukosa, kreatinin, hemoglobin dan hematokrit. Kesimpulan penelitian ini adalah penempatan dengan posisi berdiri dan berbaring di kendaraan selama 8 jam transportasi memperlihatkan respon fisiologis dan penyusutan bobot badan yang sama pada domba lokal jantan.(Physiological responses and body weight loss of male local sheep during transportation with different position on the vehicle)ABSTRACT. The aim of this study was to evaluate the physiological responses and body weight loss of males local sheep to transportation with different positions on the vehicle. Ten males sheep with 16.112.27 kg of body weight and 8-10 months in aged were used in this study. Completely Randomized Design with two factors was used in this study. The first factor was positions on the vehicle (standing and lying down) and the second factors was sampling times (before and after transportation). All of sheep were transported in one Pick up (Mitsubishi L 300) for 8 hours with similar of density level of 0,27 m2/head. Data on body weight, rectal temperature, respiration rate, pulse, cortisol, glucose, creatinine, hemoglobin and hematocrit were collected before and after transportation. Results showed there was no significant interaction (P 0.05) between the position of the sheep in the vehicle with the time of sampling in all parameters (except pulse rate). Placing of sheep with standing and lying down position for 8 hours of transportation showed the same responses for body weight loss, rectal temperature, respiratory rate, cortisol level, glucose concentration, creatinine, hemoglobin and hematocrit. Based on those findings, it can be concluded that standing and lying position in the vehicle for 8 hours of transportation shows the similar effect on the physiological responses and body weight loss of local male sheeps.
Hubungan Kadar Progesteron pada Fase Awal Luteal dengan Kematian Embrio pada Sapi Aceh Budianto Panjaitan; Citra Chyntia Helwana; Nellita Meutia; Yusmadi Yusmadi; Tongku Nizwan Siregar; Dasrul Dasrul; Teuku Armansyah TR
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14881

Abstract

ABSTRAK. Progesteron merupakan hormon yang berperan penting dalam proses pemeliharaan kebuntingan dan dihasilkan oleh corpus luteum. Penelitian ini bertujuan mengetahui hubungan antara kadar hormon progesteron pada fase awal luteal dengan kematian embrio pada sapi Aceh. Dalam penelitian ini digunakan empat ekor sapi betina dewasa berumur 3-5 tahun, bobot badan 150-250 kg, sehat secara klinis, dan memiliki reproduksi normal. Sapi disinkronisasi menggunakan 5ml prostaglandin F2 alfa (PGF2) dengan pola penyuntikan ganda berinterval 11 hari. Koleksi sampel darah untuk pengukuran konsentrasi progesteron dilakukan pada hari ke-5, 6, dan 7 pasca inseminasi. Pengukuran konsentrasi progesteron dilakukan menggunakan metode enzymelinked-immunoassay (ELISA), pemeriksaan kebuntingan dan kematian embrio menggunakan metode transrektal ultrasonografi pada hari ke-25 pasca inseminasi. Pemeriksaan diulang setiap 10hari sampai hari ke-55 pasca inseminasi. Puncak sekresi progesteron pada sapi bunting dengan embrio yang bertahan hidup terdapat pada hari ke-7 (2,082 ng/ml), pada sapi Late Embryonic Mortality (LEM) di hari ke-5 (8,209 ng/ml) dan pada sapi tidak bunting di hari ke-7 (3,0511,157 ng/ml). Sekresi progesteron sapi LEM pada hari ke-5 sampai dengan ke-7 cenderung menurun sedangkan pada sapi yang bertahan hidup cenderung meningkat.(Correlation between progesterone levels in early luteal phase and embryonic death in Aceh cattle)ABSTRACT. Progesterone is an important hormone that functions to maintain pregnancy and is produced by the corpus luteum. The aim of this study was to see a correlation between progesterone and the incidence of embryonic death in Aceh cattle. This study used four adult female cows, 3-5 years old, 150-250 kg body weight, clinically healthy, and have a normal reproduction. The synchronized with 5 ml prostaglandin F2 alfa hormone, and double injection pattern with 11-day intervals. The blood was collected for progesterone measurements on 5th, 6th, 7th day post artificial insemination. Measurement of progesterone concentration was carried out using an enzymelinked-immunoassay (ELISA), while pregnancy and embryo mortality was performed using the trans-rectal ultrasonography method on the 25th day after insemination. The examination was repeated every 10 days until day 55th after insemination. Progesterone secretion peaks in pregnant cows were on day 7th (2.082 ng/ml), in cattle Late Embryonic Mortality (LEM) on day 5th (8.209 ng/ml) and in cattle not pregnant on day 7th (3.0511.157 ng/ml). The pattern of LEM progesterone secretion on days 5th to 7th tends to decrease while those that survive tend to increase.
Hubungan antara Morfologi Ambing, Produksi Susu dan Komponen Susu pada Sapi Friesian Holstein Dian Wijayanti Solechah; Dian Wahyu Harjanti; Rudy Hartanto
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14713

Abstract

ABSTRAK. Ukuran-ukuran ambing merupakan salah satu indikator yang menentukan produksi susu sapi perah. Morfologi ambing dapat digunakan untuk menilai produktivitas ternak. Penelitian ini bertujuan untuk mengetahui hubungan antara ukuran-ukuran ambing, produksi susu dan komponen susu. Materi yang digunakan yaitu 30 ekor sapi (Friesian Holstein) FH periode laktasi III-V dan bulan laktasi 3-4. Analisis yang digunakan adalah regresi linier sederhana dengan SPSS 16. Hasil penelitian menunjukkan ada hubungan yang nyata (P 0,05) antara kedalaman ambing belakang dan panjang ambing terhadap produksi susu secara berturut-turut dengan persamaan regresi Y = -1,142 + 0,435 X (r = 0,494 dan R2 = 0,244),Y = -9,197 + 0,463 X (r = 0,625 dan R2 = 0,390), lebar ambing belakang terhadap produksi susu dan jarak antar puting depan dengan produksi susu dengan persamaan regresi secara berturut-turut Y = 1,236 + 0,28X (r = 0,397 dan R2 = 0,157) dan Y = 17,203-0,996 X (r = 0,367 dan R2 = 0,134). Kesimpulan yang didapat bahwa ada hubungan antara ukuran-ukuran ambing dengan produksi susu sapi Friesian Holstein, dimana terdapat hubungan yang signifikan antara kedalaman ambing belakang, panjang ambing serta lebar ambing belakang dan jarak antar puting depan dengan produksi susu dengan koefisien korelasi (r) tertinggi sebesar 0,625 dan koefisien determinasi (R2) sebesar 39%.(Relationship between udder morphology, milk production and milk components of friesian holstein cows)ABSTRACT. The size of the udder is one indicator that determines the production of dairy cows. The udder morphology can be used to assess livestock productivity. This study aimed to determine the relationship between udder measurements, components of milk and milk production. The material used was 30 Holstein Friesian (FH) lactation periods III-V and lactation months 3-4. The analysis used was a simple linear regression with SPSS 16. The results showed a significant relationship (P0.05) between the depth of the udder and length of the udder to milk production in a row with the regression equation Y = -1,142 + 0,435 X (r = 0,494 and R2 = 0,244), Y = -9,197 + 0,463 X (r = 0,625 and R2 = 0,390), width of the rear udder to milk production and the distance between the front nipple and milk production with consecutive regression equation Y = 1,236 + 0,28X (r = 0,397 and R2 = 0,157) and Y = 17,203 0,996 X (r = 0,367 and R2 = 0,134). The conclusion that there is a relationship between udder measurements with Holstein Friesian milk production, where there is a significant relationship between the depth of the udder, udder length and width of the udder and the distance between the front nipples with milk production with the highest correlation coefficient (r) of 0,625 and the coefficient of determination (R2) of 39%.
Fermentabilitas dan Kecernaan Ransum Domba yang Mengandung Limbah Roti secara In Vitro Asep Saripudin; Shena Nurpauza; Budi Ayuningsih; Iman Hernaman; Ana Rochana Tarmidi
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14120

Abstract

ABSTRAK. Penelitian bertujuan untuk mengetahui fermentabilitas dan kecernaan ransum domba yang mengandung limbah roti. Penelitian menggunakan rancangan acak lengkap dengan lima macam ransum perlakuan yang terdiri atas 40% rumput lapangan dan 60% konsentrat yang masing-masing mengandung limbah roti sebanyak 0 (kontrol), 10, 20, 30, dan 40%. Masing-masing perlakuan diulang sebanyak 4 kali dan data yang terkumpul dianalisis dengan analisis sidik ragam dan dilanjutkan dengan uji Duncan. Ransum perlakuan dievaluasi secara in vitro. Peubah yang diukur adalah asam lemak terbang (ALT), N-NH3, kecernaan bahan kering, dan kecernaan bahan organik. Hasil penelitian menunjukkan bahwa penggunaan limbah roti dalam ransum domba sampai 40% mampu meningkatkan konsentrasi ALT (102,63-143,88 mM), kecernaan bahan kering (64,66-78,61%) dan kecernaan bahan organik (53,41-65,82%). Sementara itu, terjadi kenaikan konsentrasi N-NH3 (3,87-4,90 mM) sampai penggunaan limbah roti 30%, namun penggunaan 40% limbah roti sama dengan ransum kontrol. Konsentrasi ALT memiliki hubungan erat dengan kecernaan bahan kering dan bahan organik dengan nilai r = 0,67 dan 0,65, pada persamaan regresi Y = 0,1591X + 50,79 dan Y = 0,1528X + 39,619. Kesimpulan, Penggunaan limbah roti di dalam ransum sebanyak 40% tidak mengganggu fermentabilitas dan kecernaan ransum secara in vitro.(In vitro fermentability and digestibility of sheep rations containing bread waste)ABSTRACT. The study aimed to determine the fermentability and digestibility of sheep rations containing bread waste. The study used a completely randomized design with five types of treatment rations consisting of 40% native grass and 60% concentrate and each treatment containing bread waste as much as 0 (control), 10, 20, 30, and 40%. Each treatment was repeated 4 times and the data collected was analyzed by analysis of variance and continued by Duncan test. The treatment ration was evaluated by in vitro. The variables measured were volatile fatty acids (VFA), N-NH3, dry matter digestibility, and organic matter digestibility. The results showed that the use of bread waste in sheep rations up to 40% was able to increase the concentration of VFA (102.63 to 143.88 mM), dry matter (64.66to78.61%) and organic matter digestibility (53.41to65.82%). Meanwhile, there was an increase in the concentration of N-NH3 (3.87 to 4.90 mM) until 30%, but the use of 40% bread waste was the same as the control ration. The VFA concentration has a close relationship with the dry matter and organic matter digestibility with values of r = 0.67 and 0.65, in the regression equation Y = 0.1591X + 50.79 and Y = 0.1528X + 39.619. The conclusion is using of bread waste as much as 40% did not disturb the fermentability and digestibility of the ration in vitro.
Efektivitas Sukrosa sebagai Proteksi Aktif Membran Ekstraseluler Spermatozoa Sapi Bali pada Zona Pre-Freezing Pajri Anwar; Jiyanto Jiyanto
Jurnal Agripet Vol 19, No 1 (2019): Volume 19, No. 1, April 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i1.14468

Abstract

ABSTRAK. Penelitian ini bertujuan untuk mengetahui efektivitas kinerja sukrosa dalam Tris kuning telur sebagai krioprotektan untuk perlindungan aktif ekstraseluler membran plasma utuh spermatozoa sapi Bali. Bahan yang digunakan dalam penelitian ini adalah sperma sapi Bali terpilih dan bahan pengencer semen. Penelitian menggunakan rancangan acak lengkap (RAL) yang terdiri dari 5 perlakuan dan 4 ulangan. Perlakuan A (Kontrol) = Tris 80%+20% Kuning telur + Gliserol; Perlakuan B = 0,2% Sukrosa + Tris 80%+20% Kuning telur + Gliserol; Perlakuan C = 0,3% Sukrosa + Tris 80%+20% Kuning telur + Gliserol; Perlakuan D = 0,4% Sukrosa + Tris 80%+20% Kuning telur + Gliserol; Perlakuan E = 0,5% Sukrosa + Tris 80%+20% Kuning telur + Griserol. Parameter yang diamati dalam penelitian ini adalah pergerakan progresif aktif spermatozoa dan membran Plasma Utuh (MPU) pada tahap pre-feezing semen sapi Bali. Hasil penelitian menunjukkan bahwa tingkat konsentrasi sukrosa pada tahap pre-freezing tidak berpengaruh nyata (P 0,05%) terhadap persentase progresif aktif spermatozoa dan proteksi MPU semen Sapi Bali dan tingkat aktif progresif spermatozoa dengan penambahan sukrosa dalam kuning telur dikategorikan baik (66.75- 69.00%). Penambahan berbagai level konsentrasi sukrosa sebagai krioprotektan ekstraseluler melapisi dan mengikat membran spermatozoa dari efek perubahan suhu yang drastis pada tahap pre-freezing dalam proses semen beku.The effectiveness of sucrose as the Active Protection of Bali Spermatozoa Extracellular Membranes Pre-FreezingABSTRACT. This study aimed to determine the effectiveness of the performance of sucrose in tris egg yolk as a cryoprotectant for extracellular active protection of extracellular membrane of Bali bulls spermatozoa. The material used in this study is the sperm of selected Bali bulls and spermatozoa thinning agent. This study used an experimental method with a completely randomized design (CRD) consisting of 5 treatments and 4 replications. Treatment A (Control) = Tris 80% + 20% Egg Yolk + Glycerol; Treatment B = 0.2% Sucrose + Tris 80% + 20% Egg yolk + Glycerol; Treatment C = 0.3% Sucrose + Tris 80% + 20% Egg yolk + Glycerol; Treatment D = 0.4% Sucrose + Tris 80% + 20% Egg yolk + Glycerol; and Treatment E = 0.5% Sucrose + Tris 80% + 20% Egg Yolk + Griserol. The parameters observed in this study were the active progressive movement and extracellular membrane of spermatozoa protection in Bali bulls pre-freezing stage. The results showed that the sucrose tris egg yolk concentration level at the pre freezing stage no significant effect (P 0.05%) on the active progressive percentage of spermatozoa and bali bulls extracellular membrane protection conditions active levels of progressive spermatozoa with the addition of sucrose in egg yolk are categorized as good (66.75- 69.00%). Addition of various levels of sucrose concentration as extracellular cryoprotectant coating and binding of spermatozoa membranes from the effects of drastic temperature changes in the pre-freezing stage in the process of frozen sperm.

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