cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Yasir Sidiq
Contact Email
ys120@ums.ac.id
Phone
+6282134901660
Journal Mail Official
bioexperimen@ums.ac.id
Editorial Address
Jl. A. Yani, Pabelan, Kartasura, Sukoharjo, Jawa Tengah 57169 Indonesia
Location
Kota surakarta,
Jawa tengah
INDONESIA
Bioeksperimen
Published by Universitas Muhammadiyah Surakarta
ISSN : 24601365     EISSN : 25272799     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Aim Bioeksperimen: Jurnal Penelitian Biologi is a peer-reviewed, open-access scientific journal dedicated to advancing knowledge across a broad spectrum of biological sciences through the publication of high-quality original research articles. The journal promotes rigorous experimental and exploratory research that advances fundamental and applied understanding in biological sciences. Bioeksperimen provides an international platform for researchers, academics, and practitioners to disseminate innovative empirical findings and scientifically robust investigations that contribute to the development of biological sciences at regional and global levels. The journal welcomes manuscripts that: 1. Present original experimental or exploratory research in biological sciences 2. Demonstrate strong methodological design and scientific rigor 3. Contribute novel data, findings, or biological insights 4. Address contemporary biological challenges and emerging scientific issues 5. Integrate laboratory, field, and computational approaches 6. Contribute meaningfully to the advancement of biological sciences All manuscripts must be original, unpublished, written in English, and must clearly demonstrate methodological soundness, data-driven analysis, and a significant contribution to biological sciences. Since March 2026, Bioeksperimen does not publish literature review articles, conceptual papers, or purely theoretical manuscripts. Focus The primary focus of Bioeksperimen: Jurnal Penelitian Biologi is on empirical biological research based on experimental, observational, or exploratory approaches. The journal prioritizes studies that: 1. Apply molecular, physiological, ecological, microbiological, or genetic techniques 2. Utilize omics-based and bioinformatics approaches to generate primary data 3. Conduct laboratory experiments, field investigations, or integrative biological exploration 4. Produce reproducible and evidence-based scientific findings Scope Bioeksperimen: Jurnal Penelitian Biologi publishes original research in the following areas: Main Topics: 1. Ecology and Environmental Sciences 2. Molecular Biology of Plants, Animals, and Microorganisms 3. Bioinformatics and Computational Biology 4. Omics-Based Studies (Genomics, Transcriptomics, Metagenomics, Proteomics, Metabolomics) 5. Basic and Applied Microbiology 6. Plant and Animal Physiology 7. Animal Behavior 8. Plant and Animal Systematics and Taxonomy 9. Genetics of Plants, Animals, and Microorganisms 10. Human Physiology
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 20 Documents
 1   2 
Search results for , issue "March 2026" : 20 Documents clear
Waterbirds as bioindicators of ecosystem health: seasonal dynamics in Tondano Lake, Indonesia Djarang, Rievo; Harindah, Gerry; Suriani, Ni Wayan; Taulu, Marthy Lingkan Stella
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15450

Abstract

Waterbird diversity in Tondano Lake reflects ecological degradation caused by water-hyacinth invasion and sedimentation. This study analyzed the diversity and abundance of waterbirds in Tondano Lake, Indonesia, across wet (February-May) and dry (June-October) seasons in 2025 to assess their role as bioindicators of ecosystem health. Multi-season observations were carried out monthly along a 6.5 km transect at ten strategic points. Eight waterbird species from three families were recorded, with Egretta garzetta being the most abundant (29.1%) and Alcedo atthis the least (4.8%). The Shannon-Wiener diversity index (H' = 1.92) indicates a moderate level of species diversity, which reflects a pattern of ecological simplification when compared to pristine tropical lakes such as Lake Sentarum (H' = 2.5). Waterbird abundance showed significant negative correlations with water hyacinth density (r = -0.68, p = 0.032) and water depth (r = -0.72, p = 0.019), revealing that invasive vegetation and shoaling are primary drivers of habitat loss. These findings demonstrate that waterbirds function as sensitive bioindicators of ecological stress, highlighting the urgent need for integrated watershed management to prevent further community collapse.
Assessment of genetic diversity in EMS-induced porang (Amorphophallus muelleri Blume) revealed by RAPD markers Haritzah, Rendha Maulana; Radita, Novia Fitri; Ramadanti, Isma Salsabila; Azzahro, Yusri Despa; Wahyudi, Didik; Resmisari, Ruri Siti; Suyono
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15768

Abstract

Increasing genetic diversity of porang through mutation is urgently needed since porang has low genetic diveristy. Therefore, this study aims to increase the genetic diversity of porang using ethyl methanesulfonate (EMS) and analyze the genetic diversity of porang using RAPD markers. Porang explants were subjected to in vitro EMS induction at various concentrations (control, 0.1%, 0.2%, 0.3%, 0.4%, and 0.5%) and evaluated after 35 days. DNA amplification was carried out using 20 RAPD primers. Genetic diversity within and between porang populations was analyzed using POPGENE software, while cluster analysis was performed using PAST software to identify genetic diversity patterns. The results showed that EMS mutation induction effectively increased the genetic diversity of porang. Primers OPA-2 and OPA-9 were identified as the most effective primers for detecting genetic diversity resulting from mutation induction. The 0.2% EMS treatment is recommended because it produced the most notable genetic changes compared to the control and shows potential for generating superior mutants to support porang breeding programs.
Integrating field inventory and in silico phylogenetic analysis of pteridophytes at Gandul Mount, Indonesia Candra, Sri Devi Galuh; Kinasih, Anggiresti; Khotimperwati, Lilih
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15947

Abstract

Pteridophytes are vascular plants that are considered the most primitive plants because they only use spores to reproduce. Several major fern families exhibit distinctive morphological and ecological characteristics that support their taxonomic recognition. Pteridophytes represent an important component of tropical biodiversity, yet their diversity and evolutionary relationships in karst ecosystems remain poorly documented. This study aimed to integrate field inventory and in silico phylogenetic analysis to assess pteridophyte diversity in Gandul Mount, Wonogiri, Central Java. Field data were collected using the cruise method, followed by morphological identification and habitat characterization. Phylogenetic relationships were inferred using matK gene sequences retrieved from the NCBI database and analyzed with the Maximum Likelihood method. The inventory recorded 17 species belonging to eight families, with Pteridaceae as the most dominant. Most species were lithophytic, indicating strong adaptation to rocky substrates. Two species were categorized as Least Concern, while the remaining species have not been evaluated by the IUCN. Eleven species showed ethnobotanical potential as medicinal plants, ornamentals, and phytoremediation agents. Phylogenetic analysis revealed clustering patterns consistent with taxonomic classification. These results demonstrate that Gandul Mount harbors considerable pteridophyte diversity and confirm that combining field-based inventory with in silico phylogenetic analysis provides a robust approach for biodiversity assessment and evolutionary interpretation.
Insect diversity at vegetative maize phase (Zea mays L.) in caturharjo village Putri, Dila Savira Mayang; Putra, Ichsan Luqmana Indra
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15963

Abstract

This study aims to analyse the diversity and dominance of insect species in maize fields in Caturharjo Village, Sleman District, Yogyakarta. The research was conducted from March to August 2025 on 1,000 m² of maize fields divided into three plots, each measuring 10 × 15 m. Light traps were installed in the centre of each plot, and insects were captured manually (direct capture) every three days. Species diversity was calculated using the Shannon-Wiener Index, dominance using the Simpson Index, and the data were analysed for correlation with abiotic factors. The results showed that the total number of insect individuals in the vegetative phase of maize plants was 19,874 individuals, consisting of 6 orders, 24 families, and 30 species. The largest proportion of insects were predators (33.33%), followed by herbivores (26.67%), maize pests (16.67%), visitors (10%), detritivores (10%), and pollinators (3.33%). The insect diversity index was (H') = 0.83, which was classified as low diversity, and the dominance index was (D) = 0.18, indicating low dominance. The conclusion of this study is that the insect species diversity at the study site was low, with no dominant species found, and a total of 30 insect species found.
Diversity of plankton species in different ecosystems on Beras Basah Island, Bontang, East Kalimantan Imam Fadila, Muhammad; Anjasmara, Aang Setyawan; Rizal, Daisy Rahma; Rusdi, Rismawaty; Reynalta, Ricko
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15978

Abstract

Beras Basah Island, located in the coastal waters of Bontang City, East Kalimantan, is a marine conservation area characterized by diverse coastal ecosystems, including marine waters, coral reefs, and seagrass beds. These ecosystems support various aquatic organisms, including plankton communities that play a key role in marine food webs. This study aimed to examine the composition, abundance, and diversity of plankton across three different ecosystems around Beras Basah Island. Sampling was conducted in May 2025 using a purposive sampling method at three stations representing marine waters, coral reefs, and seagrass bed. Plankton samples were collected using a 20 µm mesh plankton net and preserved with Lugol’s solution. The data were analyzed to determine plankton abundance, Shannon–Wiener diversity index (H’), and evenness index (E). A total of 13 plankton species were identified, consisting of 10 phytoplankton species (Bacillariophyceae and Dinophyceae) and 3 zooplankton species (Ciliata and Crustacea). The Shannon–Wiener diversity index ranged from 2.17 to 2.30, indicating moderate diversity, while evenness values ranged from 0.94 to 0.98, suggesting a relatively uniform distribution of individuals among species. Phytoplankton dominated the plankton community across all ecosystems, with the highest abundance recorded in the seagrass ecosystem (1,732 individuals L⁻¹). These findings indicate that plankton communities are relatively consistent across different coastal ecosystems in the waters of Beras Basah Island.
Pharmacological prediction of Marchantia polymorpha: GC-MS and molecular docking approaches Farhan, Abdillah Maulana
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.15983

Abstract

Marchantia polymorpha is the liverworts that can be found abundantly in the Mount Pasang Jember area. The metabolite profiling of M. polymorpha has not yet been conducted.  The method used in this study was untargeted metabolite profiling using GC-MS, and the results of the metabolite compound profiling were analyzed in silico using bioinformatics-based, namely PASS Online, SwisADME, and Molecular Docking using PyRx 8.0 with AutodockVina. The aims  of docking with this protein was adjusted to the PASS Online results, namely alkenylglycerophosphocholine hydrolase inhibitor and antiseborrheic, which are closely related to antifungals. The natural metabolite compounds detected from M. polymorpha were fatty acids (20%), terpenoids (16%), and phenolics (10%). Terpenoids-phenolics compounds were analyzed in silico to predict pharmacological potential. Terpenoids showed that the compound with the highest Pa value was 3,7-Cyclodecadiene (Pa=0.920), predicted to be an alkenylglycerophosphocholine hydrolase inhibitor, while the phenolic compound with the highest Pa value was hydroquinone (Pa=0.927), predicted to be an antiseborrheic. Based on SwissADME in silico Druglikeness, five compounds out of a total of six terpenoid and phenolic compounds showed compliance with Lipinski's theory. In addition, the in silico results also showed a bioavailability score of 0.55. Molecular docking was performed on the target protein sterol 14-α-demethylase (CYP51) from Candida albicans (PDB ID: 5TZ1). This protein is representative of antifungal agents. The results of molecular docking showed that the compound 3,7-Cyclodecadiene consistently had the strongest binding affinity value of -10.1 kcal/mol with residues ILE A:55, ALA A:62, PHE A:58, and TRP B:5  These results  imply that further research on M. polymorpha metabolites should be conducted using comprehensive methods to explore their potential in the field of health.
Evidence-based non-residential waste analysis to support 3R strategies and food recovery hierarchy: a case study in Solok Selatan Fauzi, Mhd.; Aziz, Rizki; Chyntia, Nanda; Titani, Fena Retyo; Afrianto, Andika Wahyu
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16030

Abstract

The amount of waste generation that increases without being accompanied by good waste management will cause pollution and decrease the aesthetic value of the environment. Non-residential waste originating from non-residential activities is also one of the contributors to waste entering the landfill. This study aims to analyze non-residential waste generation and composition as an evidence-based reference for each source in implementing the 3R concept and the Food Recovery Hierarchy (FRH). The number of sampling sets was determined at a minimum of 10% of each type of facility following SNI 19-3964-1994. Sampling was carried out on eight consecutive days with 14 sampling points in Area 1, 14 in Area 2, and 11 in Area 3. The total non-residential waste generation was 46.79 m³/day (9.49 tons/day). Organic waste dominated the composition, accounting for 35–37% as food waste, followed by plastic (24–29%) and paper (14–20%). The high proportion of biodegradable and recyclable materials indicates significant potential for composting, recycling, and food recovery strategies at the source level. Implementing source-level 3R and FRH approaches could substantially reduce landfill dependency and extend landfill lifespan. This study addresses a knowledge gap by linking source-level non-residential waste characterization with the practical implementation of the 3R concept and the Food Recovery Hierarchy. This study provides quantitative evidence to guide localized waste minimization strategies in non-residential sectors.
Antagonistic activity of phyllosphere fungi isolated from medicinal plants against Colletotrichum sp. causing anthracnose in chilli (Capsicum annuum L.) Puspita, Ayumi Rizci; Alamsjah, Feskaharny; Mildawati, Mildawati; Agustien , Anthoni
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16072

Abstract

Anthracnose disease caused by the fungus Colletotrichum sp. is one of the main problems in chili cultivation (Capsicum annuum L.) and can cause significant yield losses. The intensive use of chemical pesticides in controlling this disease has a negative impact on the environment and health. This study aimed to evaluate the antagonistic potential of phyllosphere fungi isolated from five medicinal plants, namely Phaleria macrocarpa, Curcuma domestica, Kleinhovia hospita, Hibiscus rosa-sinensis, and Morus macroura, against the pathogen Colletotrichum sp. Isolation was performed from leaf surfaces, and a total of 7 phyllosphere fungal isolates were tested using a double culture method with three replicates. The results showed that all isolates were able to inhibit the growth of Colletotrichum sp. through mechanisms of competition for space and nutrients as well as antibiosis, with inhibition levels ranging from high to very high. Among the tested isolates, Trichoderma sp. exhibited the highest inhibitory activity with an inhibition percentage of 92.78%, categorized as very high. Macroscopic and microscopic characterization supported the identification of several isolates belonging to the genera Trichoderma. This study concluded that phyllosphere ungi from medicinal plants have the potential as environmentally friendly biological control agents in combating anthracnose disease in chili plants.
Design and validation of PIP gene primer for quantitative PCR in Capsicum annuum using in silico and experimental approaches Nurhafitri, Amanda; Suwirmen
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16129

Abstract

Capsicum annuum L. is an economically important horticultural crop whose productivity is strongly affected by drought stress. Plasma Membrane Intrinsic Protein (PIP), a member of the aquaporin gene family involved in water transport and osmotic regulation, represents a key target for drought stress studies, requiring highly specific and efficient primers for accurate gene expression analysis using quantitative PCR (qPCR). This study aimed to design and validate PIP gene primers for qPCR in C. annuum using integrated in silico and experimental approaches. Primer design was performed using NCBI Primer-BLAST based on the CaPIP reference sequence (XM_016711608.2), followed by in silico evaluation of primer specificity and secondary structure using Primer-BLAST and OligoAnalyzer. Nine primer pairs were initially generated and evaluated based on primer length, %GC, Tm, self3’ complementarity and amplicon size. Secondary structure analysis revealed strong self-dimer formation in pair 8, whereas pair 2 showed weak secondary structure within acceptable ΔG threshold (-9 kcal/mol). Experimental validation was conducted throught gradient PCR to optimize annealing temperature, followed by agarose gel 2%. Primer 2_CaPIP produced specific and clear amplification, with an optimal annealing temperature of 57.3oC. This study provides a validated CaPIP primer set suitable for qPCR-based gene expression analysis in C. annuum, supporting future molecular studies on drought stress tolerance.
Preliminary study of acute febrile response to crude LPS extract from Salmonella typhimurium in domestic chicken (Gallus gallus domesticus) AKY strain Wijaya, Rizky Surya; Lelono, Asmoro; Oktarianti, Rike
Bioeksperimen: Jurnal Penelitian Biologi March 2026
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v12i1.16139

Abstract

Salmonella is a genus that commonly attacks the health of poultry, especially chickens. One type of poultry that is widely attacked by S. typhimurium is chickens. S. typhimurium infects the host with Lipopolysaccharides (LPS). This study aims to determine the innate immune response of domestic chickens to LPS-based vaccines S. typhimurium as the first step in vaccine development. The sample was in the form of female domestic chickens of the AKY strain which was divided into 3 groups, the control group was injected using 0.1ml of PBS (Phosphate Buffer Saline) treatment group 1 was injected with LPS with an adjuvant with a concentration of 0.5mg/kg as much as 0.1ml. Treatment group 2 was injected with LPS with an adjuvant with a concentration of 1mg/kg of 0.1ml. Injection treatment group using Incomplete Freund Adjuvant (IFA). The results obtained were that the treatment groups had an increase in temperature at the 1st hour to the highest in the 3rd hour with a temperature range of 42.5°C to 42.6°C, contrast to the control group which has a static temperature with a range of 41°C to 41.8°C. The comparison of chicken body temperature among treatment groups using a Liner Mixed Model revealed a significant difference (p < 0.001) between the treatment groups and the control group. These results indicate that subcutaneous LPS administration induces a controlled acute febrile response, which is consistent with involvement of innate inflammatory pathways.

Page 2 of 2 | Total Record : 20

 1   2 

Filter by Year

2026 2026


Reset
Filter By Issues
All Issue March 2026 March 2025 September 2025