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Ahmad Shafwan S. Pulungan
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Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Medan Jl. Willem Iskandar Psr V Medan Estate, Sumatera Utara
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JBIO: jurnal biosains (the journal of biosciences)
Published by Universitas Negeri Medan
ISSN : 24431230     EISSN : 24606804     DOI : https://doi.org/10.24114/jbio.v6i1
Core Subject : Health, Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Jurnal Biosains (JBIO) features works of exceptional significance, originality, and relevance in all areas of biological science, from molecules to ecosystems, (ie genetic, microbiology, ecology, biosystematic, biostatistic) including works at the interface of other disciplines, such as chemistry, medicine,physic and mathematics. We also welcome data-driven meta-research articles that evaluate and aim to improve the standards of research in the life sciences and beyond. Our audience is the international scientific community as well as educators, policy makers, patient advocacy groups, and interested members of the public around the world.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 263 Documents
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Effectiveness of Pineapple and Lemon Peel Extracts as Antimicrobial Paper Soap Irawati, Fitri; Nasution, Nabila Halim; Utami, Sari; Pratiwi, Nanda
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.45380

Abstract

Kesehatan masih menjadi masalah yang memprihatinkan di seluruh dunia. Hal ini juga didukung dengan respon berupa upaya mewujudkan pembangunan skala besar di bidang kesehatan dengan target pencapaiannya pada tahun 2030 yaitu Sustainable Development Goals, menjamin kehidupan yang sehat. Indonesia merupakan negara yang masih mengalami permasalahan lingkungan yang krusial, khususnya sampah. Salah satunya adalah limbah organik kulit buah nanas ( Ananas comosus L.). Penambahan ekstrak lemon ( Citrus limonL.) yang diformulasikan akan memberikan aromaterapi alami dan memberikan efek perasaan tenang. Di sisi lain, pengembangan sabun kertas merupakan inovasi efisiensi dan kemampuan untuk menciptakan kebiasaan mencuci tangan di masyarakat Indonesia. Tujuan dari penelitian ini adalah untuk mengetahui pembuatan sabun kertas dengan bahan alami yaitu limbah lemon dan nanas serta mengetahui bioaktivitas kombinasi tersebut sebagai antiseptik sabun kertas. Metode penelitian ini merupakan metode eksperimen dengan menguji aktivitas antimikroba yang dilakukan dengan metode difusi paper disc technique. Dari penelitian ini dapat disimpulkan bahwa sabun kertas yang terbuat dari bahan alami kulit lemon dan nanas dapat direalisasikan dan memiliki efek antiseptik dengan zona hambat total rata-rata terhadap S. aureus.bakteri sebesar 5,7 mm dan terhadap bakteri E. coli sebesar 20 mm sehingga sabun kertas tergolong efektif dalam menghambat pertumbuhan bakteri patogen
POTENTIAL ANALYSIS OF THE BIOLARVACIDAL PLANT ON Aedes aegypti FROM THE COMMUNITY YARD OF GUNUNGPUYUH SUB-DISTRICT, SUKABUMI CITY Hilda Munadiah; Muhammad Nur Azzura Saraesa; Jujun Ratnasari
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.40339

Abstract

Dengue hemorrhagic fever (DHF) is an infectious disease caused by the dengue virus and transmitted through the bite of the Aedes sp. mosquito. This disease is one of the main problems in Indonesia. Sukabumi is one of the cities in West Java that has the highest case di DHF. Chemical larvicides were used to control the Aedes aegypti mosquito. But its cause resistance in mosquito larva, so the seek for alternatives larvicides from natural and environmentally friendly are sought. Many studies had carried out on alternatives larvicides derived from natural product (biolarvicides). Natural larvicides have one advantage, that they do not cause resistance in mosquitoes. Several studies on biolarvicides are summarized to obtain information on several plants planted by the community in Gunungpuyuh District, Sukabumi City, which have the potential as biolarvicides. Of the 343 species of plants planted by the community, seven species of plants have high effectiveness with an LC (Median Lethal Concentration) LC50 value of <750 ppm. Lemongrass, cucumber, soursop, and kaffir lime were plants that potencial as biolarvicides compared to the others with LC50 values of 1.553 ppm, 189.261 ppm, 279.882 ppm, and 603 ppm. This plant is easy to cultivate and can be used as a biolarvicides by the community.
THE EFFECT OF KEPOK BANANA PEEL EXTRACT (Musa acuminate balbisiana Colla) AND BAP ON THE GROWTH OF RED POTATO PLANLETS (Solanum tuberosum L.) IN VITRO Edi, Syahmi; Parhusip, Tiropa Oktavia; Panggabean, Nurul Huda
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.60290

Abstract

This research aims to determine the effect of from kepok banana peel extract (Musa acuminata balbisiana Colla) and BAP and their interaction on the growth of red potato plantlets (Solanum tuberosum L.) in vitro. This research was conducted in September-October 2023 at the G10 Agrotech Medan Plant Tissue Culture Laboratory Jl. Sei Bahorok No. 47 F Medan Baru. This research used a factorial Completely Randomized Design (CRD) with two factors. The first factor is the concentration of Kepok banana peel extract in 3 levels, namely (0, 25 and 50 g/L) and the second factor is BAP which consists of 4 levels, namely (0, 0.5, 1 and 1.5 mg/L). 12 treatment combinations were obtained and each treatment was repeated 3 times to obtain 36 experimental units. The research parameters were plantlet height, number of shoots, number of leaves and number of roots analyzed using Analysis of Variance (ANOVA) and if they were significantly different, further tests would be carried out using the Duncan Multiple Range Test (DMRT) at the 5% level. The results showed that the treatment of kepok banana peel extract and BAP had a significant effect on plantlet height, number of shoots, number of leaves and number of roots. The highest average plantlet height was produced by banana peel extract 50gr/L (7.60 cm), BAP 1mg/L (6.70 cm). The highest number of shoots was produced by banana peel extract 50gr/L (6.33 shoots), BAP 1.5 mg/L (6.33 shoots). The highest number of leaves was produced by sweet corn extract 25 gr/L, namely (10.00 leaves), BAP 0.5 mg/L as much as (8.00 leaves). The highest number of roots was produced by the 50gr/L sweet corn extract treatment, namely (12.00 roots), the highest number of roots was produced by BAP 1 mg/L, namely (7.33 roots). The highest average plantlet height, number of shoots and leaves were produced by the A2B1 treatment interaction, namely 7.40 cm plantlet height, 6.33 shoots and 16.33 leaves. The highest number of roots was produced by the A2B2 treatment interaction, namely 8.67 roots.  This is an open-access article under the CC-BY-SA license                                                        
CHARACTERIZATION OF BACTERIA Salmonella sp. ON BROILER CHICKEN MEAT MARKETED AT SUKARAMAI MARKET, MEDAN CITY Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S; Silaban, Friend
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.49964

Abstract

One effort to meet high protein needs is obtained from chicken meat. Chicken meat is a food source that is composed of various nutritional values in balanced proportions. There is still doubt about Boiler Chicken meat being sold for sale because it is suspected that there is contamination with pathogenic microbes that can cause disease in humans. There is a risk that broiler chicken meat will be contaminated due to poor market sanitation. The aim of this research was to determine the bacterial contamination of Salmonella sp. in broiler chicken meat and the number of Salmonella sp bacteria. Which is found in samples of broiler chicken meat marketed at the Sukaramai Market, Medan City. This research method is a quantitative descriptive approach. This research was carried out at the Regional Health Laboratory of North Sumatra Province in May-June 2023. In this study, sample culture was carried out by streaking on Salmonella Shigella Agar (SSA) media, while the Total Plate Count (TPC) test for Salmonella sp. using pour plate media. The results showed that all 9 samples contained Salmonella sp. with the amount of bacterial contamination, namely A1 3.0 × 10-4, A2 3.9 × 10-4, A3 4.7 × 10-4, B1 4.0 × 10-4, B2 5.3 × 10-4, B3 6.0×10-4, C1 4.5×10-4, C2 6.8×10-4, C3 7.7×10-4. Based on the National Chicken Meat Standardization Agency ICS No. 67-120-20. In 2009, the maximum limit for microbial contamination and the maximum limit for residues in food ingredients of animal origin must not contain Salmonella sp.
IDENTIFICATION AND PHYTOCHEMICAL TESTS OF FUNGI MUSHROOMS AND HONEY SPECIALLY BANGKA BELITUNG Eva Dewi Rosmawati Purba; Rachmawati Felani Djuria; Rahmad Lingga
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.31428

Abstract

Pelawan Bitter Honey and Pelawan Mushroom are typical Bangka biodiversity found in Central Bangka Regency. The Pelawan mushroom grows in Bangka Belitung Islands Province and generally used as a food ingredient and has the potential to be developed as a source of natural immunomodulator agent. Phenolic compounds are known to have immunomodulatory activities that can improve the immune system. In addition, based on preliminary study, honey was also used by local people to strengthen the immune system. The purpose of this study was to identify the content of active compounds from Pelawan honey and Pelawan mushrooms. This research was an experimental study with several stages of testing including phytochemical screening, Thin Layer Chromatography and Extract Standardization tests. The results showed that based on phytochemical test of Pelawan honey contained flavonoids and alkaloids, while based on the Thin Layer Chromatography (TLC) test contained flavonoids and alkaloids. Phytochemical test of Pelawan mushroom showed the existence of Phenolic and Alkaloids, while based on TLC test containing flavonoids with an average Rf value of 0.56.  The standardization of Pelawan mushroom extract produced a total ash content of 28%, drying shrinkage of 40.88 g /mL, specific gravity 0.67 g/mL and water soluble extract content of 59.01% and ethanol soluble extract content of 41.12%. From these results, it can be assumed that the pelawan honey and pelawan mushroom have the potential to be developed as immunomodulatory agent.
DIVERSITY OF MAKROZOOBENTHOS IN THE FISH-SHRIMP POND AREAS IN ULEE MATENG VILLAGE Gilang Pradana; Chairunas Adha Putra; Mustaqim Mustaqim; Rizal Mukra; Rini Hafzari
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.60306

Abstract

Makrozoobenthos plays a crucial role in ecosystems as producers, primary consumers, and decomposers or detritus that can degrade organic compounds into inorganic ones. As primary consumers, the role of makrozoobenthos is vital for the survival of wildlife, especially various species of shorebirds commonly found in coastal areas and fish-shrimp pond areas. The aims of this research is determining the abundance of makrozoobenthos (ind/m3) and ecological index at the pond in Ulee Matang Village. The diversity of macrozoobenthos species in the area consists of 12 species of three phylum and six classes. The abundance of macrozoobenthos on two type of pond (fish and shrimp) are ranging from 892 ind/m3 to 1400 ind/m3. The Arthropoda phylum, abundant in the research area, notably featured the Water Flea Daphnia sp. that serving as a primary food source for fish and shrimp prior to the introduction of fish and shrimp seeds. This suggests a significant role of arthropods in the food chain, supporting not only fish and shrimp but also macrozoobenthos and shorebirds.
IDENTIFYING THE POTENTIAL OF Saurauia vulcani Korth. LEAVES ON BLOOD GLUCOSE LEVEL, HISTOPATHOLOGY OF PANCREATIC AND SPLEEN IN RATS (Rattus norvegicus) INDUCED ALLOXAN – IN VIVO AND IN SILICO APPROACH Erlintan Sinaga; Uswatun Hasanah; Feimmy Ruth Pratiwi Sipahutar; Hudson Sidabutar; Melati Nugrahalia Sipahutar
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 2 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i2.53242

Abstract

Insulin resistance related to pancreatic islet and spleen was one of incidence for diabetes mellitus disease. Our study determined the potential antidiabetic of Pirdot leaves (Saurauia vulcani, Korth.) (EEP) through in vivo and in silico approach. The effect of Pirdot on blood glucose level and histopathological pancreatic and spleen was analyzed by in vivo. Thirty male Wistar rats were divided into five groups as follows. Group KN served as negative control and was orally given distilled water; group KP were only 5.04 mg/ kg alloxan administered; group P1 were administered 16.5 mg/kg EEP and 5.04 mg/kg alloxan; group P2 were administered 33 mg/kg EEP and 5.04 mg/kg alloxan; group P3 rats 66 mg/kg EEP and 5.04 mg/kg alloxan. A single dose of alloxan was given in rats diabetic at KP, P1, P2, and P3 group treatment. Furthermore, the protein-protein interaction (PPI) and molecular docking were used to predict the interaction between the crossed genes in diabetes mellitus (DM) with 6 active compounds of Pirdot through in silico approach. The results indicated that Saurauia vulcani significantly decreased blood glucose and improved the histopathological alteration of pancreatic islet and spleen in alloxan induced treated diabetic rats. Moreover, the network pharmacology demonstrated ten hub genes and three genes target such as GANC (α-glucosidase), DPP4, and PTPN1 (tyrosine phosphatase protein) contributed in DM signaling pathway. Finally, the molecular docking study showed that the bioactive compounds Pirdot have a good binding affinity to the active site of PTP1B and α-glucosidase protein when compared to acarbose as a control compound
BACTERIAL OBSERVATION WITH GRAM STAIN ON PURE CULTURE OF BACTERIA IN URINE Rahel Situmorang; Zilan Syahirani; Andreas Simanjuntak
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 1 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i1.40183

Abstract

Bacteria are one of the microscopic organisms contained in the urine, and the presence of bacteria in the urine we call bacteriuria. Gram painting examination of urine with a microscope is one of the good examination methods to detect the presence of bacteriuria. This study aims to identify gram-positive bacteria and gram-negative bacteria in urine using the Gram staining method. The research method used is the experimental method. This research is in the laboratory by giving treatment to the object of study.  Sampling is urine, taken from the urine of one of the students of Medan State University. The results of the culture process are known that the urine sample (U4) has a coccus-shaped cell shape with a red cell color which indicates that the bacterial cells in this urine are included in the gram-negative type of bacteria. In gram-negative bacteria, the cell wall structure consists of three layers (the inner layer is peptidoglycan, one layer is murein base frame and the outer layer is lipopolysaccharide and lipoprotein), there is no teichoic acid, lysozyme softens the cell wall (detergents disorganize that wall by damaging the lipid layer), the cell wall is thin which is about 10-15 nm, less susceptible to penicillin, and not so absorbent of basic dyes but with safranin dyes (red) very absorb.
ACTIVITY TEST OF SIAM LABU (Sechium edule (Jacq.) Swartz) LEAF EXTRACT ON REDUCTION OF DARAGE URATE ACID TARGETS OF WHITE RATS (Rattus novergicus) Tambusai, Sofia Rahmi
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.51002

Abstract

Uric acid is a substance produced from the breakdown of purines or waste products in the body, as a result of purine catabolism aided by the enzymes cguanase and xanthine oxidase. One of the green plants  used as traditional medicine is siam labu (Sechium edule). This plant is a vegetable plant belonging to the Curcubatiacae family that grows in the highlands. The purpose of this study was to test the anti-gout activity of chayote leaves in male  rats. The method used in this study started from preparing simplisia, preparing siam labu leaf extract, inducing mice with Ca-oxanate at a dose of 250 mg/kgBW and administering siam labu leaf extract at a dose of 250 mg/kgBW, 500 mg/kgBW. and 750 mg/kgBW was administered orally with intermittent observation periods of 1 hour for 6 hours. Results obtained in rats given 750 mg/kgBW of extract showed the lowest reduction of 3.88 ± 0.3 mg/dl. One-way ANOVA results  obtained a significant value p=0.00 (p<0.05), which can be concluded that there are differences in each treatment method in experimental animals. Tukey test results can be seen at 6 hours, it can be concluded that EDLS 750 mg/kg body weight with allopurinol is not significantly different.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter

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